A Database of Drosophila Genes & Genomes

FB2008_06, released July 3, 2008
 

Gene Dmel\kl-5

General Information
SymbolDmel\kl-5SpeciesD. melanogaster
Namemale fertility factor kl5Annotation symbolCG40444
Feature typeprotein_coding_geneFlyBase IDFBgn0001315
Created / Updated2007-03-05/2007-03-05
Genomic Location
Chromosome (arm)YHetRecombination mapY-
Cytogenetic mapSequence locationYHet:311..33,413 [+]
Map ( GBrowse ) detailed view
hide Summary Information
Automatically generated summary

See sections below for more information
The gene male fertility factor kl5 is referred to in FlyBase by the symbol kl-5 (CG40444, FBgn0001315). Its sequence location is YHet:311..33413. Its molecular function is described as: microtubule binding; motor activity; ATPase activity, coupled; ATP binding; ATPase activity; microtubule motor activity; cysteine-type endopeptidase activity. It is involved in the biological processes: microtubule-based movement; proteolysis. 24 alleles are reported. The phenotypes of these alleles are annotated with: axoneme; testis; spermatid. It has one annotated transcript and one annotated polypeptide.

hide Phenotypic Description from the Red Book (Lindsley & Zimm 1992)
Gene/Allele symbols may differ from current usage
kl-5
Males mutant for kl-5 fail to assemble the outer dynein arms associated with the A tubules of the peripheral doublets of the sperm tail axoneme [Hardy, Tokuyasu, and Lindsley, 1981, Chromosoma 83: 593-617 (fig.)]. Such males also fail to produce kl-5-specific 300-325 kilodalton sperm polypeptide considered to be a component of the outer arms (Goldstein, Hardy, and Lindsley, 1982, Proc. Nat. Acad. Sci. USA 79: 7404-09). kl-5 mutants exhibit very low level of fertility at 25 but not at 18 and in young but not older males (Kennison, 1983, Genetics 103: 219-34). Deficiencies for kl-5 completely sterile, and in addition to the mutant phenotype they fail to produce aggregates of tubuli normally seen in thin sections of primary spermatocyte nuclei (Hardy et al.). Within the kl-5 fertility region of the spermatocyte nucleus, a loop-forming site has been demonstrated by its reaction with the monoclonal antibody S5 (Bonaccorsi et al., 1988). These loops are stained with the protein-specific dye CBB, but are not visible in living preparations.
hide Detailed Mapping Data
FlyBase Computed Cytological Location
Cytogenetic map
Evidence for location
h2-h3  
Left limit from complementation mapping against T(Y;3)G42 (FBrf0039037) Right limit from complementation mapping against T(Y;3)J94 (FBrf0039037)  
Experimentally Determined Cytological Location
Cytogenetic map
Notes
References
h2-h3
 
(Drosophila Heterochromatin Genome Project, 2004)
h3-h3
 
(Gepner, 1993)
h3-h3
(determined by in situ hybridisation)  
(Rasmusson et al., 1994)
Experimentally Determined Recombination Data
Location
Y-
(Hackstein and Hochstenbach, 1995)
Left of (cM)
Right of (cM)
Notes
Molecular Map Data
Gene Order (in direction of increasing cytology)
References
In direction of increasing cytology: kl-5? PRY? kl-3?
(Clark, 2001.3.5)
Gene Order (overall orientation not stated)
References
hide Gene Model & Products
Please see the GBrowse view of Dmel\kl-5 for information on other features
detailed view FBtr0113891 FBtr0113892 FBtr0113756 FBpp0112614 FBpp0112615 FBpp0112479
Comments on Gene Model
hide Transcript Data
Annotated Transcripts
Name
FlyBase ID
RefSeq ID
Length (nt)
Associated CDS (aa)
kl-5-RA
FBtr0113891
 NM_001015499
  7122
  2373
Additional Transcript Data & Comments
Reported size (kB)
Comments
External Data
Crossreferences
hide Polypeptide Data
Annotated Polypeptides
Name
FlyBase ID
Predicted MW (kD)
Length (aa)
Theoretical pI
RefSeq ID
GenBank protein
kl-5-PA  
FBpp0112614  
274.7  
2373  
5.55  
  NP_001015499
  EAL24525
Additional Polypeptide Data & Comments
Reported size (kD)
Comments
External Data
Linkouts
PANTHER - Protein classification by function, families, and pathways
•
Crossreferences
InterPro domains - A database of protein families, domains, and functional sites
•
Peptidase, cysteine peptidase active site (IPR000169)
Dynein heavy chain (IPR004273)
ATPase associated with various cellular activities, AAA-5 (IPR011704)
Dynein heavy chain, N-terminal region 1 (IPR013594)
Dynein heavy chain, N-terminal region 2 (IPR013602)
AAA+ ATPase, core (IPR003593)
hide Sequences Consistent with the Gene Model
DDBJ /
EMBL /
GenBank
DNA sequence
Protein sequence
Name
AF136243
AAF21324
 
AF136244
AAF21325
 
AF136245
AAF21326
 
AF136246
AAF21327
 
AF136247
AAF21328
 
AF136248
AAF21329
 
AF136249
AAF21330
 
AF136250
AAF21331
 
AF136251
AAF21332
 
AF136252
AAF21333
 
AF136253
AAF21334
 
AF210453
AAF21041
 
L23199
AAA52099
 
 
UniProtKB/Swiss-Prot
    UniProtKB/TrEMBL
    Maps to
    Does NOT map to
    Identified with
    hide Mapped Features & Mutations
    Please see GBrowse or insertion reports for information on insertions of transgenic constructs and features not listed here
    Type
    Symbol & Location
    Additional Notes
    References
    hide External Data
    Linkouts
    Crossreferences
    hide Expression Data
    FlyBase-Curated Data
    Transcript and
    Protein data     		Please see the FlyBase Gene Expression Report for details of gene expression from the literature.
    hide Summary of Transcript Expression
    Stage
    Tissue/Position
    Reference
     adult stage | male
     
    (Rasmusson et al., 1994)
     adult stage
     testis
    (Rasmusson et al., 1994)
    Marker for
      Subcellular Localization
      CV Term
      hide Summary of Polypeptide Expression
      Stage
      Tissue/Position
      Reference
      Marker for
        Subcellular Localization
        CV Term
        hide External Data & Images
        Linkouts
        FLIGHT - Cell culture data for RNAi and other high-throughput technologies
        •
        GEO (NCBI) - Gene expression data: microarray and other high-throughput technologies
        •
        hide Alleles & Phenotypes
        hide Summary of Allele Phenotypes
        Sterility
        Allele
        male sterile
        male sterile (with Ab(Y)Y146)
        male sterile (with Ab(Y)Y2)
        male fertile | reduced | conditional ts
        Phenotype manifest in
        Allele
        testis
        spermatid
        axoneme
        hide Classical Alleles ( 24 )
        For All Classical Alleles Show

        Allele of kl-5ClassMutagenStocksKnown lesion
        kl-5160 --
        kl-5B280 --
        kl-5B340 --
        kl-5B30 --
        kl-5E120 --
        kl-5E130 --
        kl-5E140 --
        kl-5E150 --
        kl-5E160 --
        kl-5E170 --
        kl-5G120 --
        kl-5G130 --
        kl-5G140 --
        kl-5G150 --
        kl-5G160 --
        kl-5G530 --
        kl-5R501 --
        kl-5S210 --
        kl-5X10 --
        kl-5X20 --
        kl-5X30 --
        kl-5ts10 --
        kl-5ts20 --
        kl-5ts30 --
        hide Alleles Carried on Transgenic Constructs ( 0 )
        For All Alleles Carried on Transgenic Constructs Show

        Allele of kl-5ClassMutagenStocksKnown lesion
        hide Aneuploid Aberrations
        Disrupted in
        (Zhang and Stankiewicz, 1998)
        (Hazelrigg et al., 1982)
        (Hazelrigg et al., 1982)
        (Hazelrigg et al., 1982)
        (Hazelrigg et al., 1982)
        (Hazelrigg et al., 1982)
        (Hazelrigg et al., 1982)
        (Hardy et al., 1984)
        (Hardy et al., 1984)
        (Hardy et al., 1984)
        (Hardy et al., 1984)
        (Bonaccorsi and Lohe, 1991)
        (Gatti and Pimpinelli, 1983)
        (Gatti and Pimpinelli, 1983)
        (Gatti and Pimpinelli, 1983)
        (Gatti and Pimpinelli, 1983)
        (Gatti and Pimpinelli, 1983)
        (Gatti and Pimpinelli, 1983, Gepner and Hays, 1993)
        (Gatti and Pimpinelli, 1983)
        (Gatti and Pimpinelli, 1983, Gepner and Hays, 1993)
        (Gatti and Pimpinelli, 1983)
        (Gatti and Pimpinelli, 1983)
         
         
         
         
         
         
         
         
         
         
         
        (Gatti and Pimpinelli, 1983)
        (Gatti and Pimpinelli, 1983)
        (Gatti and Pimpinelli, 1983, Bonaccorsi and Lohe, 1991)
        (Gatti and Pimpinelli, 1983)
        (Gatti and Pimpinelli, 1983, Bonaccorsi and Lohe, 1991)
        (Gatti and Pimpinelli, 1983, Bonaccorsi and Lohe, 1991)
        (Gatti and Pimpinelli, 1983)
        (Gatti and Pimpinelli, 1983)
        (Gatti and Pimpinelli, 1983)
        (Gatti and Pimpinelli, 1983)
        (Gatti and Pimpinelli, 1983)
        (Gatti and Pimpinelli, 1983, Bonaccorsi and Lohe, 1991)
        (Gatti and Pimpinelli, 1983)
        (Gatti and Pimpinelli, 1983)
         
        (Zhang and Stankiewicz, 1998)
        (Zhang and Stankiewicz, 1998)
        (Bonaccorsi et al., 1990)
        (Zhang and Stankiewicz, 1998)
        (Zhang and Stankiewicz, 1998)
        (Bonaccorsi et al., 1990)
        (Bonaccorsi et al., 1990)
        (Hazelrigg et al., 1982)
        Not disrupted in
         
        (Hazelrigg et al., 1982)
        (Hazelrigg et al., 1982)
        (Hazelrigg et al., 1982)
        (Gepner and Hays, 1993)
        (Gatti and Pimpinelli, 1983)
        (Gatti and Pimpinelli, 1983)
        (Hazelrigg et al., 1982)
        (Hazelrigg et al., 1982)
        (Gatti and Pimpinelli, 1983)
        (Gatti and Pimpinelli, 1983)
        (Hazelrigg et al., 1982)
        (Gatti and Pimpinelli, 1983)
        (Gatti and Pimpinelli, 1983)
        (Gatti and Pimpinelli, 1983)
        (Gatti and Pimpinelli, 1983)
        (Hazelrigg et al., 1982)
        (Gatti and Pimpinelli, 1983)
        (Hazelrigg et al., 1982)
        (Gatti and Pimpinelli, 1983)
        (Gatti and Pimpinelli, 1983)
        (Gatti and Pimpinelli, 1983)
        (Gatti and Pimpinelli, 1983)
        (Gatti and Pimpinelli, 1983)
        (Gatti and Pimpinelli, 1983)
        (Hazelrigg et al., 1982)
        (Gatti and Pimpinelli, 1983)
        (Gatti and Pimpinelli, 1983)
        (Gatti and Pimpinelli, 1983)
        (Gatti and Pimpinelli, 1983)
        (Zhang and Stankiewicz, 1998)
        (Zhang and Stankiewicz, 1998)
        (Gepner and Hays, 1993)
        (Gepner and Hays, 1993)
        (Gepner and Hays, 1993)
        (Gepner and Hays, 1993)
        (Bonaccorsi et al., 1990)
        (Bonaccorsi et al., 1990)
        (Hazelrigg et al., 1982)
        (Hazelrigg et al., 1982)
        Duplicated in
        (Gepner and Hays, 1993)
        (Gepner and Hays, 1993)
        (Gepner and Hays, 1993)
        (Gepner and Hays, 1993)
        hide Transgenic Constructs & Insertions
        Transgenic Constructs
        Type of construct
        Name
        Expression data
        Insertions
        Type of insertions
        Name
        Expression data
        insertion of enhancer trap
        P{PZ}kl-516
        No
        hide Related Comments
        Please look at the allele reports for the complete phenotype data
        Males mutant for kl-5 fail to assemble the outer dynein arms associated with the A tubules of the peripheral doublets of the sperm tail axoneme (Hardy, Tokuyasu, and Lindsley, 1981). Such males also fail to produce kl-5-specific 300-325 kilodalton sperm polypeptide considered to be a component of the outer arms (Goldstein, Hardy and Lindsley, 1982). kl-5 mutants exhibit very low level of fertility at 25oC but not at 18oC and in young but not older males (Kennison, 1983). Deficiencies for kl-5 completely sterile and in addition to the mutant phenotype they fail to produce aggregates of tubuli normally seen in thin sections of primary spermatocyte nuclei (Hardy et al., 1981). Within the kl-5 fertility region of the spermatocyte nucleus, a loop-forming site has been demonstrated by its reaction with the monoclonal antibody S5 (Bonaccorsi et al., 1988). These loops are stained with the protein-specific dye CBB, but are not visible in living preparations.
         
        AAGAC satellite DNA repeats which map to the kl-5 and ks-1 loop forming regions are abundantly transcribed in primary spermatocytes. These transcripts are detected at all stages of development of these two loops, do not appear to migrate to the cytoplasm and are degraded when the loops disintegrate during the first meiotic prophase.
        (Bonaccorsi et al., 1990)
        Primary spermatocyte nuclei exhibit three giant lampbrush-like loops formed by the kl-5, kl-3 and ks-1 Y chromosome fertility factors.
        (Pisano et al., 1993)
        kl-5 gene locus encodes a dynein heavy chain, the dynein heavy chain sequences are localised to the kl-5 region of the Y chromosome. These results provide evidence that at least one of the Y chromosome fertility regions encodes a polypeptide required for spermatogenesis.
        (Gepner and Hays, 1993)
        hide Gene Ontology: Function, Process & Cellular Component ( 11 )
        hide Molecular Function
        CV term
        Evidence
        References
        ATP binding
        inferred from electronic annotation with InterPro:IPR011704
        (FlyBase Curators et al., 2004-)
        ATPase activity
        inferred from electronic annotation with InterPro:IPR011704
        (FlyBase Curators et al., 2004-)
        ATPase activity, coupled
        inferred from sequence or structural similarity
        (Goldstein and Gunawardena, 2000)
        cysteine-type endopeptidase activity
        inferred from electronic annotation with InterPro:IPR000169
        (FlyBase Curators et al., 2004-)
        microtubule binding
        inferred from sequence or structural similarity
        (Kurek et al., 1999)
        inferred from sequence or structural similarity with SGD_LOCUS:DYN1; SGD:S0001762
        (FlyBase, 1992-)
        microtubule motor activity
        inferred from electronic annotation with InterPro:IPR004273
        (FlyBase Curators et al., 2004-)
        motor activity
        inferred from sequence or structural similarity
        (Kurek et al., 1999)
        inferred from sequence or structural similarity with SGD_LOCUS:DYN1; SGD:S0001762
        (FlyBase, 1992-)
        hide Biological Process
        CV term
        Evidence
        References
        microtubule-based movement
        inferred from sequence or structural similarity
        (Goldstein and Gunawardena, 2000)
        inferred from electronic annotation with InterPro:IPR004273
        (FlyBase Curators et al., 2004-)
        proteolysis
        inferred from electronic annotation with InterPro:IPR000169
        (FlyBase Curators et al., 2004-)
        hide Cellular Component
        CV term
        Evidence
        References
        axonemal dynein complex
        inferred from sequence or structural similarity
        (Kurek et al., 1999)
        inferred from sequence or structural similarity
        (Goldstein and Gunawardena, 2000)
        inferred from sequence or structural similarity with SGD_LOCUS:DYN1; SGD:S0001762
        (FlyBase, 1992-)
        microtubule associated complex
        non-traceable author statement
        (Gepner, 1993)
        inferred from sequence or structural similarity with SGD_LOCUS:DYN1; SGD:S0001762
        (FlyBase, 1992-)
        hide Sequence Ontology: Class of Gene
        protein_coding_gene
         
        nuclear_gene
         
        hide Interactions & Pathways
        hide Summary of Genetic Interactions
        Interacts with
        Please look at the allele data for full details of the genetic interactions
        kl-5 allele
        Gene
        References
        hide External Data
        Linkouts
        BioGRID - Interaction data, including yeast 2-hybrid and genetic interactions
        •
        hide Orthologs
        Genome-wide drosophilid orthologs
        Curated drosophilid orthologs
         
         
         
         
         
        (Kurek et al., 2000)
        (Kurek et al., 1998)
        (Zurovcova and Eanes, 1999)
         
        (Carvalho et al., 2006, Carvalho and Clark, 2005)
        (Carvalho et al., 2006)
        (Llopart et al., 2005)
        (Zurovcova and Eanes, 1999)
         
         
        (Zurovcova and Eanes, 1999)
        (Carvalho et al., 2006)
        (Zurovcova and Eanes, 1999)
        Linkouts
        hide Functional Complementation between Species
        hide Inter-Species Misexpression Data
        Produces phenotype in
        Produces NO phenotype in
        hide Stocks & Reagents
        hide Stocks Listed in FlyBase ( 1 )
        Bloomington
        2763
        T(Y;2)R50, kl-5R50 BS, y+/SM1; C(1)RM, y1/C(1;Y)1, y1
        hide Genomic Clones ( 0 )
        hide cDNA Clones ( 0 )
        cDNA Clones, Fully Sequenced
        BDGP DGC clones
        Other clones
        cDNA Clones, End Sequenced (ESTs)
        BDGP DGC clones
        Other clones
        hide RNAi & Array Information
        Affy Oligo
        Linkouts
        DRSC - RNAi screening (Harvard): high-throughput cell culture data and design
        •
        hide Antibody Information
        hide Other Information
        hide Discoverer
        hide Etymology
        hide Identification
        hide Position Effect Variegation Data
        hide Relationship to Other Genes
        Source for database identity of
        Source for database merge of
        Source for merge of: kl-5 Dhc-Yh3
        (Reugels et al., 1999)
        Source for merge of: CG12573 CG17616 Annotations CG12573 and CG17616 merged as CG40444 in release 3 of the genome annotation.
        (FlyBase Genome Annotators, 2002-2003)
        Additional comments
        Williamson (1972) reported 26 non-complementing and nine complementing alleles induced by EMS.
         
        hide Comments About Role
        hide Comments About Molecular Function
        hide Other Comments
        kl-5 has been cloned and characterised.
        (Rasmusson et al., 1994)
        Identification: PCR screen for Dynein heavy chain genes.
        (Kurek et al., 1998)
        dsRNA made from templates generated with primers directed against this gene tested in RNAi screen for effects on Kc167 and S2R+ cell morphology.
        (Kiger et al., 2003)
        hide External Crossreferences & Linkouts
        Sequence Crossreferences
        RefSeq (Transcripts)
        •
        RefSeq (Proteins)
        •
        DDBJ / EMBL / GenBank
        •