A Database of Drosophila Genes & Genomes

FB2008_06, released July 3, 2008
 

Gene Dmel\kl-2

General Information
SymbolDmel\kl-2SpeciesD. melanogaster
Namemale fertility factor kl2Annotation symbolCG17866
Feature typeprotein_coding_geneFlyBase IDFBgn0001313
Created / Updated2007-03-05/2007-03-05
Genomic Location
Chromosome (arm)YHetRecombination mapY-
Cytogenetic mapSequence locationYHet:81,411..151,168 [+]
Map ( GBrowse ) detailed view
hide Summary Information
Automatically generated summary

See sections below for more information
The gene male fertility factor kl2 is referred to in FlyBase by the symbol kl-2 (CG17866, FBgn0001313). Its sequence location is YHet:81411..151168. Its molecular function is described as: motor activity; ATPase activity, coupled; ATP binding; hydrolase activity, acting on acid anhydrides, catalyzing transmembrane movement of substances; microtubule motor activity. It is involved in the biological processes: cytoskeleton organization and biogenesis; microtubule-based movement; protein targeting; proton transport. 16 alleles are reported. The phenotypes of these alleles are annotated with: Nebenkern; spermatid; spermatocyte. It has one annotated transcript and one annotated polypeptide.

hide Phenotypic Description from the Red Book (Lindsley & Zimm 1992)
Gene/Allele symbols may differ from current usage
kl-2
Males deficient or mutant for kl-2 are sterile; primary ultrastructural lesion not identified. Such sterile males lack a 300-325 kilodalton sperm polypeptide thought to be a structural component of the axoneme (Goldstein, Hardy, and Lindsley, 1982, Proc. Nat. Acad. Sci. USA 79: 7404-09; Hardy, Lindsley, Livak, Lewis, Sivertsen, Joslyn, Edwards, and Bonaccorsi, 1984, Genetics 107: 591-610).
hide Detailed Mapping Data
FlyBase Computed Cytological Location
Cytogenetic map
Evidence for location
h10-h11  
Left limit from complementation mapping against T(Y;2)R155 (FBrf0039037) Right limit from complementation mapping against In(YL)S16L (FBrf0039037)  
Experimentally Determined Cytological Location
Cytogenetic map
Notes
References
h10-h11
 
(Drosophila Heterochromatin Genome Project, 2004)
Experimentally Determined Recombination Data
Location
Y-
 
Left of (cM)
Right of (cM)
Notes
Molecular Map Data
Gene Order (in direction of increasing cytology)
References
Gene Order (overall orientation not stated)
References
hide Gene Model & Products
Please see the GBrowse view of Dmel\kl-2 for information on other features
detailed view FBtr0113762 FBpp0112485
Comments on Gene Model
hide Transcript Data
Annotated Transcripts
Name
FlyBase ID
RefSeq ID
Length (nt)
Associated CDS (aa)
kl-2-RA
FBtr0113762
 NM_001015505
  11725
  3907
Additional Transcript Data & Comments
Reported size (kB)
Comments
External Data
Crossreferences
hide Polypeptide Data
Annotated Polypeptides
Name
FlyBase ID
Predicted MW (kD)
Length (aa)
Theoretical pI
RefSeq ID
GenBank protein
kl-2-PA  
FBpp0112485  
452.9  
3907  
6.84  
  NP_001015505
  EAL24531
Additional Polypeptide Data & Comments
Reported size (kD)
Comments
External Data
Linkouts
Crossreferences
InterPro domains - A database of protein families, domains, and functional sites
•
ATPase, F0 complex, subunit A (IPR000568)
Protein secE/sec61-gamma protein (IPR001901)
Dynein heavy chain (IPR004273)
ATPase associated with various cellular activities, AAA-5 (IPR011704)
Dynein heavy chain, N-terminal region 1 (IPR013594)
Dynein heavy chain, N-terminal region 2 (IPR013602)
AAA+ ATPase, core (IPR003593)
hide Sequences Consistent with the Gene Model
DDBJ /
EMBL /
GenBank
DNA sequence
Protein sequence
Name
AF313479
AAG29545
 
CO325719
 
DQ239724
ABB76244
 
DQ239731
ABB76248
 
 
UniProtKB/Swiss-Prot
    UniProtKB/TrEMBL
    Maps to
    Does NOT map to
    Identified with
    hide Mapped Features & Mutations
    Please see GBrowse or insertion reports for information on insertions of transgenic constructs and features not listed here
    Type
    Symbol & Location
    Additional Notes
    References
    hide External Data
    Linkouts
    Crossreferences
    hide Expression Data
    FlyBase-Curated Data
    Transcript and
    Protein data     		Please see the FlyBase Gene Expression Report for details of gene expression from the literature.
    hide Summary of Transcript Expression
    Stage
    Tissue/Position
    Reference
    Marker for
      Subcellular Localization
      CV Term
      hide Summary of Polypeptide Expression
      Stage
      Tissue/Position
      Reference
      Marker for
        Subcellular Localization
        CV Term
        hide External Data & Images
        Linkouts
        FLIGHT - Cell culture data for RNAi and other high-throughput technologies
        •
        hide Alleles & Phenotypes
        hide Summary of Allele Phenotypes
        Sterility
        Allele
        male sterile
        Phenotype manifest in
        Allele
        Nebenkern
        spermatid
        spermatocyte
        hide Classical Alleles ( 16 )
        For All Classical Alleles Show

        Allele of kl-2ClassMutagenStocksKnown lesion
        kl-2A33
          		0 --
          kl-2A49
            		0 --
            kl-2B370 --
            kl-2B380 --
            kl-2E50 --
            kl-2E60 --
            kl-2E70 --
            kl-2E80 --
            kl-2G70 --
            kl-2G80 --
            kl-2S16L0 --
            kl-2S180 --
            kl-2X10 --
            kl-2ts10 --
            kl-2ts20 --
            kl-2ts30 --
            hide Alleles Carried on Transgenic Constructs ( 0 )
            For All Alleles Carried on Transgenic Constructs Show

            Allele of kl-2ClassMutagenStocksKnown lesion
            hide Aneuploid Aberrations
            Disrupted in
            (Hazelrigg et al., 1982)
            (Hazelrigg et al., 1982)
            (Hazelrigg et al., 1982)
            (Hazelrigg et al., 1982)
            (Hardy et al., 1984)
            (Hardy et al., 1984)
            (Hardy et al., 1984)
            (Hardy et al., 1984)
            (Hardy et al., 1984)
            (Hardy et al., 1984)
            (Bonaccorsi and Lohe, 1991)
            (Gatti and Pimpinelli, 1983)
            (Gatti and Pimpinelli, 1983)
            (Gatti and Pimpinelli, 1983)
            (Gatti and Pimpinelli, 1983)
            (Gatti and Pimpinelli, 1983)
            (Hazelrigg et al., 1982)
            (Gatti and Pimpinelli, 1983, Hardy et al., 1984)
             
             
             
            (Gatti and Pimpinelli, 1983, Hardy et al., 1984)
            (Zhang and Stankiewicz, 1998)
             
            (Zhang and Stankiewicz, 1998)
            (Bonaccorsi et al., 1990)
            (Bonaccorsi et al., 1990)
            (Bonaccorsi et al., 1990)
            (Bonaccorsi et al., 1990)
            (Hazelrigg et al., 1982)
            Not disrupted in
            (Hazelrigg et al., 1982)
            (Hazelrigg et al., 1982)
            (Hazelrigg et al., 1982)
            (Hazelrigg et al., 1982)
            (Hazelrigg et al., 1982)
            (Gatti and Pimpinelli, 1983)
            (Gatti and Pimpinelli, 1983)
            (Gatti and Pimpinelli, 1983)
            (Gatti and Pimpinelli, 1983)
            (Gatti and Pimpinelli, 1983)
            (Hazelrigg et al., 1982)
            (Hazelrigg et al., 1982)
            (Gatti and Pimpinelli, 1983)
            (Gatti and Pimpinelli, 1983)
            (Gatti and Pimpinelli, 1983)
            (Gatti and Pimpinelli, 1983)
            (Gatti and Pimpinelli, 1983)
            (Hazelrigg et al., 1982)
            (Gatti and Pimpinelli, 1983)
            (Hazelrigg et al., 1982)
            (Gatti and Pimpinelli, 1983)
            (Gatti and Pimpinelli, 1983)
            (Gatti and Pimpinelli, 1983)
            (Gatti and Pimpinelli, 1983)
            (Gatti and Pimpinelli, 1983)
            (Gatti and Pimpinelli, 1983)
            (Hazelrigg et al., 1982)
            (Gatti and Pimpinelli, 1983)
            (Gatti and Pimpinelli, 1983)
            (Gatti and Pimpinelli, 1983)
            (Gatti and Pimpinelli, 1983)
            (Gatti and Pimpinelli, 1983)
            (Gatti and Pimpinelli, 1983)
            (Zhang and Stankiewicz, 1998)
            (Zhang and Stankiewicz, 1998)
            (Zhang and Stankiewicz, 1998)
            (Zhang and Stankiewicz, 1998)
            (Hazelrigg et al., 1982)
            (Hazelrigg et al., 1982)
            Partially disrupted in
            (Gatti and Pimpinelli, 1983)
            hide Transgenic Constructs & Insertions
            Transgenic Constructs
            Type of construct
            Name
            Expression data
            Insertions
            Type of insertions
            Name
            Expression data
            hide Related Comments
            Please look at the allele reports for the complete phenotype data
            Males deficient or mutant for kl-2 are sterile; primary ultrastructural lesion not identified. Such sterile males lack a 300-325 kilodalton sperm polypeptide thought to be a structural component of the axoneme (Goldstein, Hardy and Lindsley, 1982; Hardy, Lindsley, Livak, Lewis, Sivertsen, Joslyn, Edwards and Bonaccorsi, 1984).
             
            hide Gene Ontology: Function, Process & Cellular Component ( 13 )
            hide Molecular Function
            CV term
            Evidence
            References
            ATP binding
            inferred from electronic annotation with InterPro:IPR011704
            (FlyBase Curators et al., 2004-)
            ATPase activity, coupled
            inferred from sequence or structural similarity with EMBL:AJ242523; protein_id:CAB99316 AND traceable author statement
            (de Carvalho et al., 2000)
            hydrolase activity, acting on acid anhydrides, catalyzing transmembrane movement of substances
            inferred from electronic annotation with InterPro:IPR000568
            (FlyBase Curators et al., 2004-)
            microtubule motor activity
            inferred from electronic annotation with InterPro:IPR004273
            (FlyBase Curators et al., 2004-)
            motor activity
            inferred from sequence or structural similarity with EMBL:AL034345; protein_id:CAB41297.1
            (FlyBase, 1992-)
            hide Biological Process
            CV term
            Evidence
            References
            cytoskeleton organization and biogenesis
            inferred from electronic annotation
            (Roth and Foulger, 2002.4.24)
            microtubule-based movement
            inferred from electronic annotation
            (King et al., 2002.9.23)
            inferred from electronic annotation with InterPro:IPR004273
            (FlyBase Curators et al., 2004-)
            protein targeting
            inferred from electronic annotation with InterPro:IPR001901
            (FlyBase Curators et al., 2004-)
            proton transport
            inferred from electronic annotation with InterPro:IPR000568
            (FlyBase Curators et al., 2004-)
            hide Cellular Component
            CV term
            Evidence
            References
            axonemal dynein complex
            inferred from sequence or structural similarity with EMBL:AL034345; protein_id:CAB41297.1
            (FlyBase, 1992-)
            dynein complex
            traceable author statement
            (de Carvalho et al., 2000)
            microtubule associated complex
            inferred from sequence or structural similarity with EMBL:AL034345; protein_id:CAB41297.1
            (FlyBase, 1992-)
            proton-transporting two-sector ATPase complex
            inferred from electronic annotation with InterPro:IPR000568
            (FlyBase Curators et al., 2004-)
            hide Sequence Ontology: Class of Gene
            protein_coding_gene
             
            nuclear_gene
             
            hide Interactions & Pathways
            hide Summary of Genetic Interactions
            Interacts with
            Please look at the allele data for full details of the genetic interactions
            kl-2 allele
            Gene
            References
            hide External Data
            Linkouts
            BioGRID - Interaction data, including yeast 2-hybrid and genetic interactions
            •
            hide Orthologs
            Genome-wide drosophilid orthologs
            Curated drosophilid orthologs
            (Kopp et al., 2005.10.14)
            (Kopp et al., 2005.10.14)
            (Kopp et al., 2005.10.14)
            (Kopp et al., 2005.10.14)
            (Kopp et al., 2005.10.14)
            (Carvalho and Clark, 2005)
            (Kopp et al., 2005.10.14)
            (Kopp et al., 2005.10.14)
            (Kopp et al., 2005.10.14)
            Linkouts
            InParanoid orthologs - Eukaryotic orthologs
            •
            hide Functional Complementation between Species
            hide Inter-Species Misexpression Data
            Produces phenotype in
            Produces NO phenotype in
            hide Stocks & Reagents
            hide Stocks Listed in FlyBase ( 0 )
            hide Genomic Clones ( 0 )
            hide cDNA Clones ( 0 )
            cDNA Clones, Fully Sequenced
            BDGP DGC clones
            Other clones
            cDNA Clones, End Sequenced (ESTs)
            BDGP DGC clones
            Other clones
            hide RNAi & Array Information
            Affy Oligo
            Linkouts
            DRSC - RNAi screening (Harvard): high-throughput cell culture data and design
            •
            hide Antibody Information
            hide Other Information
            hide Discoverer
            hide Etymology
            hide Identification
            hide Position Effect Variegation Data
            hide Relationship to Other Genes
            Source for database identity of
            Source for database merge of
            Source for merge of: kl-2 CG17866 Source for merge of kl-2 CG17866 was sequence comparison (date:001108).
            (FlyBase, 1996-)
            Additional comments
            Williamson (1972) reported five EMS induced complementing alleles.
             
            hide Comments About Role
            hide Comments About Molecular Function
            hide Other Comments
            Transiently named CG40438 in release 3 of the genome annotation.
            (FlyBase Genome Annotators, 2002-2003)
            hide External Crossreferences & Linkouts
            Sequence Crossreferences
            RefSeq (Transcripts)
            •
            RefSeq (Proteins)
            •
            DDBJ / EMBL / GenBank
            •
            UniProtKB/TrEMBL
            •
            Other Crossreferences
            InterPro domains - A database of protein families, domains, and functional sites
            •
            ATPase, F0 complex, subunit A (IPR000568)
            Protein secE/sec61-gamma protein (IPR001901)
            Dynein heavy chain (IPR004273)
            ATPase associated with various cellular activities, AAA-5 (IPR011704)
            Dynein heavy chain, N-terminal region 1 (IPR013594)
            Dynein heavy chain, N-terminal region 2 (IPR013602)
            AAA+ ATPase, core (IPR003593)
            Linkouts
            BioGRID - Interaction data, including yeast 2-hybrid and genetic interactions
            •
            DRSC - RNAi screening (Harvard): high-throughput cell culture data and design
            •
            FLIGHT - Cell culture data for RNAi and other high-throughput technologies
            •
            FlyMine - Integrated genomics database for Drosophila, Anopheles, and C.elegans
            •
            InParanoid orthologs - Eukaryotic orthologs
            •
            hide Synonyms & Secondary IDs ( 6 )
            Reported As
            Symbol Synonym
            CG17866
            (Drosophila Heterochromatin Genome Project, 2007)
            CG40438
             
            kl-2
            (Koerich et al., 2007, Carvalho and Clark, 2005, Larracuente and Clark, 2008)
            Lms2
            (Hazelrigg et al., 1982, )
            Name Synonym
            1β-dynein heavy chain
            (Gvozdev et al., 2005)
            male fertility factor kl2
             
            Secondary FlyBase IDs
            • FBgn0039968
            • FBgn0058438
            • FBan0017866
            hide References ( 54 )
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