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Gene Dmel\Jra

General Information
Symbol	Dmel\Jra	Species	D. melanogaster
Name	Jun-related antigen	Annotation symbol	CG2275
Feature type	protein_coding_gene	FlyBase ID	FBgn0001291
Created / Updated	2003-12-02/2003-12-02
Genomic Location
Chromosome (arm)	2R	Recombination map	2-60
Cytogenetic map	46E4-46E5	Sequence location	2R:5,983,985..5,986,110 [+]
Map ( GBrowse )	Decorated FastA GenBankGFF Gene region Extended Gene region CDSIntronsExonsTranslationsTranscripts3' UTR5' UTR
Summary Information
Automatically generated summary See sections below for more information	The gene Jun-related antigen is referred to in FlyBase by the symbol Jra (CG2275, FBgn0001291). It has the cytological map location 46E4-46E5. Its sequence location is 2R:5983985..5986110. Its molecular function is described as: RNA polymerase II transcription factor activity; transcription factor binding; protein binding; specific RNA polymerase II transcription factor activity; protein heterodimerization activity; sequence-specific DNA binding; protein dimerization activity; transcription factor activity. It is involved in the biological processes described with 12 unique terms, many of which group under: anatomical structure development; regulation of metabolic process; MAPKKK cascade; sensory organ development; eggshell chorion formation; organ morphogenesis; establishment of planar polarity; transcription, DNA-dependent; protein kinase cascade; ovarian follicle cell development. 26 alleles are reported. The phenotypes of these alleles are annotated with 27 unique terms, many of which group under: peripheral nervous system; organ system; nervous system; adult; portion of tissue; egg; integumentary system; chorion; imaginal precursor; rhabdomere; morphogenetic furrow; commissure. It has 2 annotated transcripts and 2 annotated polypeptides.
External Summaries
Detailed Mapping Data
FlyBase Computed Cytological Location
Cytogenetic map Evidence for location 46E4-46E5   Limits computationally determined from genome sequence between P{lacW}Adamk13906&P{EP}Pka-R2EP2162 and P{PZ}14-3-3ζ07103
Experimentally Determined Cytological Location
Cytogenetic map Notes References 46E-46E (determined by in situ hybridisation)   (Zhang et al., 1990) 46E-46E (determined by in situ hybridisation)   (Perkins et al., 1990) 46E-46E (determined by in situ hybridisation)   (Perkins et al., 1988)
Experimentally Determined Recombination Data
Location	2-60 (Nusslein-Volhard et al., 1984, Tearle and Nusslein-Volhard, 1987)
Left of (cM)
Right of (cM)
Notes
Molecular Map Data
Gene Order (in direction of increasing cytology) References Gene Order (overall orientation not stated) References
Gene Model & Products
Please see the GBrowse view of Dmel\Jra for information on other features
Comments on Gene Model
Transcript Data
Annotated Transcripts
Name FlyBase ID RefSeq ID Length (nt) Associated CDS (aa) Jra-RA FBtr0088411  NM_057238   1565   289 Jra-RB FBtr0088410  NM_165739   1614   289
Additional Transcript Data & Comments
Reported size (kB)	1.7 (northern blot) (Perkins et al., 1990, Zhang et al., 1990)
Comments
External Data
Crossreferences
Polypeptide Data
Annotated Polypeptides
Name FlyBase ID Predicted MW (kD) Length (aa) Theoretical pI RefSeq ID GenBank protein Jra-PA   FBpp0087499   31.0   289   10.06     NP_476586   AAF58845 Jra-PB   FBpp0087498   31.0   289   10.06     NP_724882   AAM71059
Additional Polypeptide Data & Comments
Reported size (kD)	289 (aa); 40 (kD observed) (Perkins et al., 1990) 289 (aa) (Zhang et al., 1990)
Comments
External Data
Linkouts	PANTHER - Protein classification by function, families, and pathways • FBgn0001291
Crossreferences	InterPro domains - A database of protein families, domains, and functional sites • cAMP response element binding (CREB) protein (IPR001630) Transcription factor Jun (IPR002112) Basic-leucine zipper (bZIP) transcription factor (IPR004827) Jun-like transcription factor (IPR005643) Eukaryotic transcription factor, Skn-1-like, DNA-binding (IPR008917) bZIP transcription factor, bZIP-1 (IPR011616) TRANSFAC - Eukaryotic transcription factors, their genomic binding sites, and DNA-binding profiles • T01132 T01996
Sequences Consistent with the Gene Model
DDBJ / EMBL / GenBank	DNA sequence Protein sequence Name AA941201   AC005469   AC005974   AE003831 AAF58845   AE003831 AAM71059   AI513713   AI517601   AW942721   AY058562 AAL13791   BF495562   BF496150   BF496387   BI170369   BI170596   BI227477   BI229034   BI229036   BI352126   BI619733   BI632402   BI635556   BI637989   BP558817   CO152887   CO155570   CO156502   CO156703   CO178625   CO266863   CO291527   CO309797   CO310008   CO338774   EC056139   EC087463   EC201988   EC212330   EC218629   EC223955   EC227748   EC244995   EC245117   EC262941   EC264017   EC265394   EC267614   L33044   M36181 AAA28650   X54144 CAA38083   Y12573 CAA73154
UniProtKB/Swiss-Prot	P18289
UniProtKB/TrEMBL
Maps to	DS05181 (FlyBase, 1992-)
Does NOT map to
Identified with	LD25202 (BDGP Project Members, 2000-) D1269 (BDGP Project Members, 1994-1999)
Mapped Features & Mutations
Please see GBrowse or insertion reports for information on insertions of transgenic constructs and features not listed here Type Symbol & Location Additional Notes References enhancer Jra-enhancer-6 2R:5,987,150..5,987,155 comment=Description: Jun-related antigen; putative evidence=experimental standard_name=ecdysone site enhancer Jra-enhancer-1 2R:5,984,049..5,984,054 comment=Description: Jun-related antigen; putative evidence=experimental standard_name=ecdysone site enhancer Jra-enhancer-2 2R:5,984,140..5,984,145 comment=Description: Jun-related antigen; putative evidence=experimental standard_name=ecdysone site enhancer Jra-enhancer-3 2R:5,984,338..5,984,344 evidence=experimental standard_name=AP-1 comment=Description: Jun-related antigen; putative enhancer Jra-enhancer-4 2R:5,984,361..5,984,368 comment=Description: Jun-related antigen; putative evidence=experimental standard_name=AP-1 enhancer Jra-enhancer-5 2R:5,984,414..5,984,421 comment=Description: Jun-related antigen; putative evidence=experimental standard_name=DTF-1 point mutation Jra[IA109] 2R:5,984,985..5,984,985 evidence=experimental pr_change=K72@ na_change=A5984985T (Hou et al., 1997) protein binding site Jra-protein_bind-1 2R:5,984,413..5,984,435 evidence=experimental (Bergman et al., 2004, Wang and Goldstein, 1994) rescue fragment Jra[+t5.2] 2R:5,982,204..5,987,075 linked_to=XhoI-XbaI_rfrag evidence=experimental (Hou et al., 1997) sequence variant Jra[1] 2R:5,985,300..5,985,302 evidence=experimental pr_change=F177@ (Kockel et al., 1997)
External Data
Linkouts	DEDB - Drosophila exon database: splicing graphs • 13131
Crossreferences
Expression Data
FlyBase-Curated Data
Transcript and Protein data	Please see the FlyBase Gene Expression Report for details of gene expression from the literature.
Summary of Transcript Expression
Stage Tissue/Position Reference  embryonic stage   (Zhang et al., 1990)  embryonic stage-adult stage   (Zhang et al., 1990)
Marker for
Subcellular Localization
CV Term
Summary of Polypeptide Expression
Stage Tissue/Position Reference  larval stage | third instar  cone cell (FlyBase, 1992-)  larval stage | third instar  photoreceptor cell (FlyBase, 1992-)  larval stage | third instar  morphogenetic furrow | posterior to (FlyBase, 1992-)  larval stage | third instar  mystery cell (FlyBase, 1992-)  embryonic stage | stage >=12  dorsal ectoderm (Kockel et al., 1997)  embryonic stage  cephalic furrow (Kockel et al., 1997)  larval stage | third instar  ommatidial cluster (FlyBase, 1992-)  embryonic stage  amnioserosa (Kockel et al., 1997)
Marker for
Subcellular Localization
CV Term	nucleus (FlyBase, 1992-)
Microarray Data
	Developmental timecourse, Costello et al., 2008 (Original data from Arbeitman et al., 2002) AA941201
External Data & Images
Linkouts	FLIGHT - Cell culture data for RNAi and other high-throughput technologies • FBgn0001291 FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array • FBgn0001291 GEO (NCBI) - Gene expression data: microarray and other high-throughput technologies • FBgn0001291
Alleles & Phenotypes
Summary of Allele Phenotypes
Lethality Allele lethal (with Df(2R)X1) Jra76-19 lethal | embryonic stage | recessive Jra1 Jra76-19 JraIA109 JradsRNA.cNa lethal | embryonic stage, with Scer\GAL4pnr-MD237 JraAsp.B.Scer\UAS lethal | prepupal stage, with Scer\GAL4ey.PH JraAsp.B.Scer\UAS Other Phenotypes Allele wild-type Jra+t5.2 visible JraAsp.hs.sev visible | dominant JraAsp.hs.sev visible, with Scer\GAL4pnr-MD237 JradsRNA.Scer\UAS neurophysiology defective, with Scer\GAL4elav-C155 JraJbz.Scer\UAS neuroanatomy defective, with Scer\GAL4elav-C155 JraJbz.Scer\UAS tissue polarity | dominant JraAsp.hs.sev tissue polarity | somatic clone Jra76-19 JraIA109 Phenotype manifest in Allele embryonic dorsal epidermis Jra1 Jra76-19 JraIA109 ommatidium | somatic clone Jra76-19 JraIA109 photoreceptor cell R3 | somatic clone Jra76-19 JraIA109 photoreceptor cell R4 | somatic clone | supernumerary Jra76-19 JraIA109 head Jra76-19 JraIA109 photoreceptor cell JraAD.hs.sev.T:Hsim\VP16 eye JraAla.hs.sev JraAsp.hs.sev JrabZIP.hs.sev.T:Ivir\HA1 eye photoreceptor cell JraAla.hs.sev JrabZIP.hs.sev.T:Ivir\HA1 photoreceptor cell R7 JraAla.hs.sev JraAsp.hs.sev JraS82A.T92A.T107A.hs.sev commissure, with Scer\GAL4sim.PS JraAsp.Scer\UAS cone cell JraAsp.hs.sev ommatidium JraAsp.hs.sev JrabZIP.hs.sev.T:Ivir\HA1 eye photoreceptor cell | ectopic JraAsp.hs.sev rhabdomere JraAsp.hs.sev photoreceptor cell R7 | supernumerary JraAsp.hs.sev photoreceptor cell R4 JraAsp.hs.sev JraS82A.T92A.T107A.hs.sev eye disc JraAsp.hs.sev ommatidial cluster JraAsp.hs.sev embryonic/first instar larval cuticle | conditional ts JraAsp.hs.sev epidermis | dorsal JraIA109 embryo | anterior | dorsal JraIA109 embryonic epidermis | dorsal JraIA109 dorsal appendage | maternal effect | somatic clone JraIA109 micropyle | maternal effect | somatic clone JraIA109 dorsal closure embryo JraIA109 photoreceptor cell R1 JraS82A.T92A.T107A.hs.sev photoreceptor cell R2 JraS82A.T92A.T107A.hs.sev photoreceptor cell R3 JraS82A.T92A.T107A.hs.sev photoreceptor cell R5 JraS82A.T92A.T107A.hs.sev photoreceptor cell R6 JraS82A.T92A.T107A.hs.sev adult thorax, with Scer\GAL4pnr-MD237 JradsRNA.Scer\UAS embryonic/first instar larval cuticle | dorsal JradsRNA.cNa bouton, with Scer\GAL4elav-C155 JraJbz.Scer\UAS neuromuscular junction & larva, with Scer\GAL4elav-C155 JraJbz.Scer\UAS eye, with Scer\GAL4unspecified JraJbz.Scer\UAS
Classical Alleles ( 5 )
For All Classical Alleles Show
Allele of Jra Class Mutagen Stocks Known lesion Jra1 loss of function ethyl methanesulfonate 0 Yes Jra3 0 -- Jra76-19 amorph ethyl methanesulfonate diepoxybutane γ ray 1 Yes JraIA109 amorph, loss of function ethyl methanesulfonate 2 Yes JraRC46 0 --
Alleles Carried on Transgenic Constructs ( 21 )
For All Alleles Carried on Transgenic Constructs Show
Allele of Jra Class Mutagen Stocks Known lesion Jra+t5.2 in vitro construct | genomic fragment 0 Yes JraAD.hs.sev.T:Hsim\VP16 in vitro construct | coding region fusion 0 Yes JraAla.B.Scer\UAS in vitro construct | regulatory fusion 0 Yes JraAla.hs.sev antimorph in vitro construct | regulatory fusion in vitro construct | amino acid replacement 0 Yes JraAsp.B.Scer\UAS in vitro construct | regulatory fusion 0 Yes JraAsp.Scer\UAS in vitro construct | regulatory fusion 0 Yes JraAsp.hs.sev in vitro construct | regulatory fusion in vitro construct | amino acid replacement 0 Yes JraGD4400 in vitro construct | RNAi in vitro construct | regulatory fusion 1 Yes JraJbz.Scer\UAS antimorph in vitro construct | regulatory fusion 0 Yes JraS82A.T92A.T107A.hs.sev in vitro construct | amino acid replacement in vitro construct | regulatory fusion 0 Yes JraS82D.T92D.T107D.hs.sev in vitro construct | amino acid replacement in vitro construct | regulatory fusion 0 Yes JraScer\UAS.T:Zzzz\TAP in vitro construct | regulatory fusion in vitro construct | coding region fusion 0 Yes JraScer\UAS.cEa in vitro construct | regulatory fusion 1 Yes JrabZIP.hs.sev.T:Ivir\HA1 antimorph in vitro construct | regulatory fusion in vitro construct | coding region fusion in vitro construct | deletion 0 Yes JracRa in vitro construct | other 0 Yes JradsRNA.Scer\UAS in vitro construct | regulatory fusion in vitro construct | RNAi 0 Yes JradsRNA.cBa.Scer\UAS in vitro construct | regulatory fusion in vitro construct | RNAi 0 Yes JradsRNA.cNa in vitro construct | RNAi 0 Yes Jrafl.hs.sev.T:Ivir\HA1 antimorph in vitro construct | regulatory fusion in vitro construct | coding region fusion 0 Yes Jrahs.T:Ecol\dam,T:Hsap\MYC in vitro construct | regulatory fusion in vitro construct | coding region fusion 0 Yes Jrahs.sev in vitro construct | regulatory fusion 0 Yes
Aneuploid Aberrations
Useful deficiency	Df(2R)stan1
Useful duplication	Dp(2;2)Y3b
Disrupted in	Df(2R)BSC303 (Christensen and Cook, 2007.5.8) Df(2R)E73 (Kockel et al., 1997) Df(2R)X1 (Goldstein et al., 1998, Goldstein et al., 2001, Riesgo-Escovar and Hafen, 1997, Carney et al., 1997)
Not disrupted in	Ab(2R)55-20 (Goldstein et al., 2001) Ab(2R)89-22 (Goldstein et al., 2001) Df(2R)12 (Carney et al., 1997) Df(2R)520 (Goldstein et al., 2001) Df(2R)61-25 (Goldstein et al., 2001) Df(2R)64-10 (Goldstein et al., 2001) Df(2R)71-81 (Goldstein et al., 2001) Df(2R)X3 (Goldstein et al., 2001, Carney et al., 1997) Df(2R)en-A (Chase and Baker, 1995) Df(2R)en-B (Chase and Baker, 1995) Df(2R)eve (Goldstein et al., 2001) Df(2R)stan2 (Goldstein et al., 2001)
Transgenic Constructs & Insertions
Transgenic Constructs
	Type of construct Name Expression data characterization construct P{Jra+t5.2} NA P{hs.sev-Jra::kay.FJF} NA P{hs.sev-Jra::kay.JFJ} NA heat-shock construct P{VP16-D-jun} NA P{sE-D-junS82A,T92A,T107A} NA P{sE-D-junS82D,T92D,T107D} NA P{sE-dm-jun} NA P{sE-junAla} NA P{sE-junAsp} NA P{sE-junbZip} NA P{sE-junwt} NA UAS construct P{GD4400} NA P{Jbz} NA P{UAS-Jra.RNAi} NA P{UAS-Jra.TAP} NA P{UAS-Jra} NA P{UAS-act-jun} NA P{UAS-jun.B.RNAi} NA P{UAS-junAla.B} NA P{UAS-junAsp.B} NA
Insertions
	Type of insertions Name Expression data
Related Comments
	Please look at the allele reports for the complete phenotype data S2 cells treated with dsRNA generated against this gene show reduced phagocytosis of Candida albicans compared to untreated cells. (Stroschein-Stevenson et al., 2006) The potential interplay of Jra with kay may play an important role in cell-type specific transcription during embryonic development. (Perkins et al., 1990) Jra has been cloned, sequenced, and its expression pattern analysed. (Zhang et al., 1990) The AP-1 complex encodes two proteins that have functional and structural properties in common with mammalian Fos and Jun proto-oncogene products; kay and Jra, respectively. The biochemical properties of the Jra gene product have been examined in vitro and the expression pattern in developing embryos studied. (Perkins et al., 1990) Jra is required downstream of the sev signalling pathway for development in the eye. (Bohmann et al., 1994) The DebA upstream region contains a binding site for the Jra/kay (AP-1) protein complex, which negatively regulates its transcription. (Goldstein, 1995) Jra cooperates with the ETS domain pnt gene product to act on common target genes which induce photoreceptor R7 fate in the developing eye. phyl might be one of the target genes. (Treier et al., 1995) Jra is a substrate for rl, part of the receptor tyrosine kinase signal transduction pathway which triggers