Gene Dmel\inv

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General Information

Symbol

Dmel\inv

Species

D. melanogaster

Name

invected

Annotation symbol

CG17835

Feature type

protein_coding_gene

FlyBase ID

FBgn0001269

Created / Updated

2005-01-19/2005-01-19

Genomic Location

Chromosome (arm)

Recombination map

Cytogenetic map

47F15-47F17

Sequence location

2R:7,361,970..7,394,688 [+]

Map ( GBrowse )

Summary Information

Automatically generated summary

See sections below for more information

The gene invected is referred to in FlyBase by the symbol inv (CG17835, FBgn0001269). It has the cytological map location 47F15-47F17. Its sequence location is 2R:7361970..7394688. Its molecular function is described as: RNA polymerase II transcription factor activity; sequence-specific DNA binding; transcription factor activity. It is involved in the biological processes: compartment specification; neuroblast fate determination; regulation of transcription from RNA polymerase II promoter; wing disc anterior/posterior pattern formation. 34 alleles are reported. The phenotypes of these alleles are annotated with 11 unique terms, many of which group under: adult segment; organ system; adult mesothoracic segment; anatomical structure; thoracic segment; adult; cuticle; integumentary system; wing blade; embryonic nervous system; embryonic hindgut; acellular anatomical structure. It has 4 annotated transcripts and 4 annotated polypeptides.

External Summaries

Phenotypic Description from the Red Book (Lindsley & Zimm 1992)

Gene/Allele symbols may differ from current usage

inv: invected

Although a developmental role for inv is indicated by hybridization, experiments with embryonic and larval cells using cDNA probes, the function in development of the protein encoded by inv is not known (Coleman et al., 1987). No mutant alleles were reported.

Detailed Mapping Data

FlyBase Computed Cytological Location

Cytogenetic map

Evidence for location

47F15-47F17

Limits computationally determined from genome sequence between P{lacW}Tapδ^k17005&P{lacW}k05103 and P{lacW}l(2)k13403^k13403&P{lacW}Egm^k14708

Experimentally Determined Cytological Location

Cytogenetic map

Notes

References

Experimentally Determined Recombination Data

Location

Left of (cM)

Right of (cM)

Notes

Molecular Map Data

Gene Order (in direction of increasing cytology)

References

In direction of increasing cytology: inv+ en-

(van Dijk and Murre, 1994, Goldsborough and Kornberg, 1994)

In direction of increasing cytology: Drip? anon-48Ab? E(Pc)? inv?

(Sinclair et al., 1998)

In direction of increasing cytology: E(Pc)+ inv+

(Stankunas et al., 1998)

In direction of increasing cytology: inv+ en- tou-

(Fauvarque et al., 2001)

Gene Order (overall orientation not stated)

References

Overall orientation not stated: Drip? anon-48Ab? E(Pc)? inv? en? tou? anon-48Ae? anon-48Af? anon-48Ag? anon-48Ah? anon-48Ai? anon-48Aj?

(Drees et al., 1987)

Gene Model & Products

Please see the GBrowse view of Dmel\inv for information on other features

Comments on Gene Model

Transcript Data

Annotated Transcripts

Name

FlyBase ID

RefSeq ID

Length (nt)

Associated CDS (aa)

inv-RA

FBtr0088067

NM_165837

3354

576

inv-RB

FBtr0088069

NM_078975

2267

576

inv-RC

FBtr0088070

NM_165838

2211

576

inv-RD

FBtr0088068

NM_165839

3407

576

Additional Transcript Data & Comments

Reported size (kB)

2.7 (northern blot)

(Coleman et al., 1987)

Comments

External Data

Crossreferences

Polypeptide Data

Annotated Polypeptides

Name

FlyBase ID

Predicted MW (kD)

Length (aa)

Theoretical pI

RefSeq ID

GenBank protein

inv-PA

FBpp0087173

60.9

576

6.25

NP_725055

AAM68709

inv-PB

FBpp0087175

60.9

576

6.25

NP_523699

AAF58640

inv-PC

FBpp0087176

60.9

576

6.25

NP_725056

AAM68707

inv-PD

FBpp0087174

60.9

576

6.25

NP_725057

AAM68708

Additional Polypeptide Data & Comments

Reported size (kD)

576 (aa); 60 (kD predicted)

(Coleman et al., 1987)

Comments

External Data

Linkouts

Crossreferences

InterPro domains - A database of protein families, domains, and functional sites

•

Helix-turn-helix motif, lambda-like repressor (IPR000047)

'Homeobox' engrailed-type protein (IPR000747)

Homeobox (IPR001356)

Homeodomain-related (IPR012287)

Homeodomain engrailed related (IPR015703)

Homeodomain-like (IPR009057)

Sequences Consistent with the Gene Model

DDBJ /
EMBL /
GenBank

DNA sequence

Protein sequence

Name

P05527

UniProtKB/TrEMBL

Maps to

Does NOT map to

Identified with

(BDGP Project Members, 2000-)

bs29f08

(Oliver, 2001.8.16)

Mapped Features & Mutations

Please see GBrowse or insertion reports for information on insertions of transgenic constructs and features not listed here

Type

Symbol & Location

Additional Notes

References

External Data

Linkouts

DEDB - Drosophila exon database: splicing graphs

•

12911

Crossreferences

Expression Data

FlyBase-Curated Data

Transcript and
Protein data

Please see the FlyBase Gene Expression Report for details of gene expression from the literature.

Summary of Transcript Expression

Stage

Tissue/Position

Reference

embryonic stage | contracted germ band stage

hindgut primordium

(Coleman et al., 1987)

embryonic stage | extended germ band

(Coleman et al., 1987)

embryonic stage | contracted germ band

(Coleman et al., 1987)

larval stage | third instar

dorsal mesothoracic disc | posterior compartment

(Coleman et al., 1987)

Marker for

Subcellular Localization

CV Term

Summary of Polypeptide Expression

Stage

Tissue/Position

Reference

Marker for

Subcellular Localization

CV Term

Microarray Data

Developmental timecourse, Costello et al., 2008 (Original data from Arbeitman et al., 2002)

Untitled Document detailed view

AI114261

External Data & Images

Linkouts

FLIGHT - Cell culture data for RNAi and other high-throughput technologies

•

FBgn0001269

FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array

•

FBgn0001269

GEO (NCBI) - Gene expression data: microarray and other high-throughput technologies

•

FBgn0001269

FlyExpress - Embryonic expression images (BDGP data)

•

Show all FlyExpress processed images for inv

Alleles & Phenotypes

Summary of Allele Phenotypes

Lethality

Allele

viable

inv³⁰

Other Phenotypes

Allele

visible

inv^unspecified

visible | dominant

visible | dominant | homeotic | conditional - heat sensitive

inv^W

Phenotype manifest in

Allele

segment

inv^12A-11

cuticle

inv^12A-11

embryonic hindgut

inv³⁰

anterior crossvein

inv³⁰

wing

wing vein | ectopic

inv^E

wing | anterior/posterior compartment boundary

inv^E

longitudinal connective

inv^E

commissure & embryo

inv^E

embryonic muscle system

inv^E

wing vein L1

inv^W

wing vein L2

inv^W

wing | anterior

inv^W

wing vein

wing | anterior/posterior compartment boundary (with inv^unspecified)

inv^W

denticle belt

inv^hs.PG

wing | anterior/posterior compartment boundary (with inv^W)

inv^unspecified

Classical Alleles ( 29 )

For All Classical Alleles Show

Allele of inv	Class	Mutagen	Stocks	Known lesion
inv^+I.Br	wild-type allele	natural population	0	--
inv^+I.GK	wild-type allele	natural population	0	--
inv¹¹			0	--
inv^12A-11	loss of function	Δ2-3	0	Yes
inv^12A		P-element activity	0	Yes
inv³⁰	amorph, loss of function	Δ2-3	1	Yes
inv^E	amorph		1	Yes
inv^KG04405		P-element activity	1	--
inv^W	gain of function	X ray	2	Yes
inv^WR3		γ ray	0	--
inv^WR4		γ ray	0	Yes
inv^WRK1		γ ray	0	Yes
inv^WRK2		γ ray	0	Yes
inv^WRK3		γ ray	0	--
inv^WRK4		γ ray	0	--
inv^WRK5		γ ray	0	--
inv^WRK6		γ ray	0	--
inv^Xba21		pπ25.7wc	0	Yes
inv^c01071		piggyBac transposase	0	--
inv^c01072		piggyBac transposase	0	--
inv^d01166		P-element activity	1	--
inv^e00137		piggyBac transposase	1	--
inv^e02196		piggyBac transposase	1	--
inv^e04219		piggyBac transposase	1	--
inv^e04243		piggyBac transposase	1	--
inv^f01496		piggyBac transposase	1	--
inv^f06310		piggyBac transposase	1	--
inv^l77		X ray	0	Yes
inv^unspecified			0	--

Alleles Carried on Transgenic Constructs ( 5 )

For All Alleles Carried on Transgenic Constructs Show

Allele of inv	Mutagen	Stocks	Known lesion
inv^Act5C.PG	in vitro construct \| regulatory fusion	0	Yes
inv^GD14791	in vitro construct \| RNAi in vitro construct \| regulatory fusion	0	Yes
inv^GD16474	in vitro construct \| RNAi in vitro construct \| regulatory fusion	2	Yes
inv^Scer\UAS.cSa	in vitro construct \| regulatory fusion	0	Yes
inv^hs.PG	in vitro construct \| regulatory fusion	0	Yes

Aneuploid Aberrations

Useful deficiency

Df(2R)en-SFX31

Useful duplication

Dp(2;2)Y3b

Disrupted in

Df(2R)en-11

(Sanicola et al., 1995)

Df(2R)en-SFX31

(Serrano et al., 1995, Whiteley and Kassis, 1997)

Df(2R)en^C

(Saenz-Robles et al., 1995)

Df(2R)en^D

(Saenz-Robles et al., 1995)

Df(2R)en^E

(Lawrence et al., 1999, Cadigan et al., 1994, Hidalgo, 1994, Zecca et al., 1995, Tabata et al., 1995, Gustavson et al., 1996, Martin-Bermudo and Brown, 1996, Matsuzaki and Saigo, 1996, Strutt and Mlodzik, 1996, Azpiazu et al., 1996, Chen and Baker, 1997, Lawrence et al., 1996, Whiteley and Kassis, 1997, Blair and Ralston, 1997)

In(2R)vg^W

(Williams et al., 1990, Simmonds et al., 1997)

In(2R)vg^WR1

(Williams et al., 1990)

In(2R)vg^WR3

(Williams et al., 1990)

In(2R)vg^WR4

(Williams et al., 1990)

In(2R)vg^WRK1

(Simmonds et al., 1997)

In(2R)vg^WRK2

(Simmonds et al., 1997)

In(2R)vg^WRK3

(Simmonds et al., 1997)

In(2R)vg^WRK4

(Simmonds et al., 1997)

In(2R)vg^WRK5

(Simmonds et al., 1997)

In(2R)vg^WRK6

(Simmonds et al., 1997)

T(2;?)inv^l77

(Gustavson et al., 1996)

Not disrupted in

Df(2R)en^F

(Gustavson et al., 1996)

Transgenic Constructs & Insertions

Transgenic Constructs

Type of construct

Name

Expression data

UAS construct

P{GD14791}

P{GD16474}

P{UAS-inv.S}

characterization construct

P{mos-en-Δ12A-11}

heat-shock construct

P{hsp70-inv}

Insertions

Type of insertions

Name

Expression data

miscellaneous insertions

P{SUPor-P}inv^KG04405

P{en-lacZ(wXba)}inv^Xba21

P{mos-en-Δ12A-11}inv^12A-11

P{mos-en}inv^12A

insertion of enhancer trap

P{lacW}65

insertion of mobile activating element

P{XP}inv^d01166

Related Comments

Please look at the allele reports for the complete phenotype data

The varied phenotypes of en alleles can be explained by the differential effects these mutants have on the combination of en and inv activities.

(Gustavson et al., 1996)

Gene Ontology: Function, Process & Cellular Component ( 8 )

Molecular Function

CV term

Evidence

References

RNA polymerase II transcription factor activity

inferred from sequence or structural similarity with EMBL:AF213356

(FlyBase, 1992-)

sequence-specific DNA binding

inferred from electronic annotation with InterPro:IPR001356

(FlyBase Curators et al., 2004-)

transcription factor activity

inferred from electronic annotation with InterPro:IPR000047, InterPro:IPR001356

(FlyBase Curators et al., 2004-)

Biological Process

CV term

Evidence

References

compartment specification

inferred from mutant phenotype AND inferred from sequence or structural similarity with FLYBASE:en; FB:FBgn0000577

(Simmonds and Bell, 1995)

traceable author statement

(Milan and Cohen, 2000)

neuroblast fate determination

inferred from genetic interaction with FLYBASE:en; FB:FBgn0000577, FLYBASE:gsb; FB:FBgn0001148 AND inferred from genetic interaction with FLYBASE:en; FB:FBgn0000577, FLYBASE:gsb; FB:FBgn0001148, FLYBASE:ptc; FB:FBgn0003892

(Bhat and Schedl, 1997)

regulation of transcription from RNA polymerase II promoter

inferred from sequence or structural similarity with EMBL:AF213356

(FlyBase, 1992-)

wing disc anterior/posterior pattern formation

traceable author statement

(Milan and Cohen, 2000)

Cellular Component

CV term

Evidence

References

nucleus