Gene Dmel\iav

Help

Close All Open All

General Information

Symbol

Dmel\iav

Species

D. melanogaster

Name

inactive

Annotation symbol

CG4536

Feature type

protein_coding_gene

FlyBase ID

FBgn0086693

Created / Updated

2007-07-20/2007-07-20

Genomic Location

Chromosome (arm)

Recombination map

1-18.8

Cytogenetic map

6D3-6D3

Sequence location

X:6,708,151..6,712,189 [+]

Map ( GBrowse )

Summary Information

Automatically generated summary

See sections below for more information

The gene inactive is referred to in FlyBase by the symbol iav (CG4536, FBgn0086693). It has the cytological map location 6D3. Its sequence location is X:6708151..6712189. Its molecular function is described as: calcium channel activity; ion channel activity. It is involved in the biological processes: calcium ion transport; sensory perception of smell; sensory perception of sound; flight behavior; response to heat; behavioral response to cocaine; ion transport. 15 alleles are reported. No phenotypic data is available. It has one annotated transcript and one annotated polypeptide.

Phenotypic Description from the Red Book (Lindsley & Zimm 1992)

Gene/Allele symbols may differ from current usage

iav: inactive (J.C. Hall)

iav1 noted (Kaplan, 1977) to be extremely inactive as adult, with normal external morphology; population of mutants remains quiet and spread out evenly in a container; will walk or fly when container disturbed, but settles into inactivity soon afterward. iav2 noted (Homyk and Sheppard, 1977) to be inactive and difficult to arouse for flight (and, when it does, seems to hover and fixate on objects); to jump and fly abnormally short distances; to exhibit slow running or climbing ability and optomotor response; to be debilitated after mechanical shock (especially in adults more than two weeks old). Gynandromorph analysis of iav2 adult behavioral impairments suggests primary focus could be neural (Homyk, 1977). Open-field activity tests of iav1 and iav2 (O'Dell and Burnet, 1988) showed both to exhibit reduced speed as well as amount of locomotion (the former meaning number of squares in the activity chambered visited/unit time, the latter the proportion of time spent moving); male activity levels higher than for females (also found for wild-type); lower than wildtype levels of activity at all ages between time of eclosion and approximately one-month; yet, unlike wild type, speed and amount of activity increase (in tests of iav2) in parallel between days 1 and 2 of adulthood (reaching a much lower than normal plateau). In tests of homozygous iav1 and iav2 females (with normal males), mating propensity reduced and courtship durations extended, though such females showed normal compositions of cuticular hydrocarbons and were highly attractive to courting males (O'Dell et al., 1989); mutant males with normal females exhibited slightly reduced mating success; mutant males crossed with mutant females had very low mating success rate. Lower than normal octopamine levels, especially in extracts from adult heads (O'Dell et al., 1987), with dopamine and norepinephrine levels apparently normal.

Detailed Mapping Data

FlyBase Computed Cytological Location

Cytogenetic map

Evidence for location

6D3-6D3

Limits computationally determined from genome sequence between P{EP}C3G^EP1613 and P{EP}EP1388^EP1388&P{EP}EP1573

Experimentally Determined Cytological Location

Cytogenetic map

Notes

References

6C5-6E4

Determined by deficiency and duplication mapping.

(Gong et al., 2004)

Experimentally Determined Recombination Data

Location

1-18.8

Left of (cM)

Right of (cM)

Notes

Based on mapping of iav¹; iav^hypoB-1 mapped to 1-20.7.

Molecular Map Data

Gene Order (in direction of increasing cytology)

References

Gene Order (overall orientation not stated)

References

Gene Model & Products

Please see the GBrowse view of Dmel\iav for information on other features

Comments on Gene Model

Transcript Data

Annotated Transcripts

Name

FlyBase ID

RefSeq ID

Length (nt)

Associated CDS (aa)

iav-RA

FBtr0070969

NM_132125

3372

1123

Additional Transcript Data & Comments

Reported size (kB)

Comments

External Data

Crossreferences

Polypeptide Data

Annotated Polypeptides

Name

FlyBase ID

Predicted MW (kD)

Length (aa)

Theoretical pI

RefSeq ID

GenBank protein

iav-PA

FBpp0070930

124.7

1123

5.98

NP_572353

AAF46203

Additional Polypeptide Data & Comments

Reported size (kD)

Comments

External Data

Linkouts

Crossreferences

InterPro domains - A database of protein families, domains, and functional sites

•

Ion transport (IPR005821)

Ankyrin (IPR002110)

Sequences Consistent with the Gene Model

DDBJ /
EMBL /
GenBank

DNA sequence

Protein sequence

Name

Q9W3W0

Maps to

Does NOT map to

Identified with

Mapped Features & Mutations

Please see GBrowse or insertion reports for information on insertions of transgenic constructs and features not listed here

Type

Symbol & Location

Additional Notes

References

deletion

iav[3621]
X:6,709,627..6,709,638

comment=Deletion of 12 base pairs encoding amino acid residues TFAQ from exon 3 of iav.
evidence=experimental

(Gong et al., 2004)

point mutation

iav[1]
X:6,709,828..6,709,828

evidence=experimental
na_change=C6709828T
pr_change=Q455@|iav-PA
reported_na_change=C1363T
reported_pr_change=Q455@

(Gong et al., 2004)

External Data

Linkouts

Crossreferences

Expression Data

FlyBase-Curated Data

Transcript and
Protein data

Please see the FlyBase Gene Expression Report for details of gene expression from the literature.

Summary of Transcript Expression

Stage

Tissue/Position

Reference

Marker for

Subcellular Localization

CV Term

Summary of Polypeptide Expression

Stage

Tissue/Position

Reference

Marker for

Subcellular Localization

CV Term

External Data & Images

Linkouts

Alleles & Phenotypes

Summary of Allele Phenotypes

Other Phenotypes

Allele

short lived

behavior defective

iav¹

hypoactive | adult stage

courtship defective | female

mating defective

auditory system defective | male

auditory system defective | female (with iav¹)

iav³⁶²¹

auditory system defective | female (with iav³⁶²¹)

iav¹

heat stress response defective

iav^hypoB-1

chemical sensitive

Phenotype manifest in

Allele

Classical Alleles ( 10 )

For All Classical Alleles Show

Allele of iav	Class	Mutagen	Stocks	Known lesion
iav¹	hypomorph	spontaneous	2	--
iav³⁶²¹		ethyl methanesulfonate	1	Yes
iav^N+			0	--
iav^N-			0	--
iav^S+			0	--
iav^S-			0	--
iav^hypoB-1		ethyl methanesulfonate	0	--
iav^hypoB-2		ethyl methanesulfonate	0	--
iav^hypoB-3		ethyl methanesulfonate	0	--
iav^unspecified			0	--

Alleles Carried on Transgenic Constructs ( 5 )

For All Alleles Carried on Transgenic Constructs Show

Allele of iav	Mutagen	Stocks	Known lesion
iav^+t9.2	in vitro construct \| genomic fragment	0	Yes
iav^{Cterm.T:Avic\GFP-EGFP}	in vitro construct \| coding region fusion	0	Yes
iav^GD3260	in vitro construct \| RNAi in vitro construct \| regulatory fusion	2	Yes
iav^{Nterm.T:Avic\GFP-EGFP}	in vitro construct \| coding region fusion	0	Yes
iav^dsRNA.cRa	in vitro construct \| RNAi	0	Yes

Aneuploid Aberrations

Useful deficiency

T(1;3)143-3

Useful duplication

Dp(1;1)GH65

Disrupted in

Df(1)pod1^Δ17

(Rothenberg et al., 2004)

Df(1)pod1^Δ291

(Rothenberg et al., 2004)

Df(1)pod1^Δ96

(Rothenberg et al., 2004)

Duplicated in

Tp(1;3)sn^13a1

(Gong et al., 2004)

Not disrupted in

Df(1)Sxl-bt

(Gong et al., 2004)

Transgenic Constructs & Insertions

Transgenic Constructs

Type of construct

Name

Expression data

UAS construct

P{GD3260}

characterization construct

P{iav-GFP.Cterm}

P{iav-GFP.Nterm}

Insertions

Type of insertions

Name

Expression data

Related Comments

Please look at the allele reports for the complete phenotype data

Mutants of iav show reductions in locomotor activity and exhibit poor mating success. Levels of the putative neurotransmitter octapamine are 10% that of wild type. In a courtship paradigm iav mutant males show an abnormal learning phenotype as a consequence of the octapamine deficiency.

(O'Dell, 1993)

Mutant flies fail to become sensitized to cocaine.

(McClung and Hirsh, 1999)

Larvae simultaneously injected with dsRNAs against iav and nan show normal thermotactic behaviour.

(Rosenzweig et al., 2005)

Gene Ontology: Function, Process & Cellular Component ( 10 )

Molecular Function

CV term

Evidence

References

calcium channel activity

inferred from sequence or structural similarity

(Littleton and Ganetzky, 2000)

inferred from sequence or structural similarity with EMBL:AJ133128; protein_id:CAB40138.1

(FlyBase, 1992-)

ion channel activity

non-traceable author statement

(Kim et al., 2003)

Biological Process

CV term

Evidence

References

behavioral response to cocaine

non-traceable author statement

(Rothenfluh and Heberlein, 2002)

calcium ion transport

inferred from sequence or structural similarity

(Littleton and Ganetzky, 2000)

flight behavior

inferred from mutant phenotype

(Homyk and Sheppard, 1977)

ion transport

inferred from electronic annotation with InterPro:IPR005821

(FlyBase Curators et al., 2004-)

response to heat

inferred from mutant phenotype

(Chentsova et al., 2002)

sensory perception of smell

inferred from sequence or structural similarity

(Littleton and Ganetzky, 2000)

sensory perception of sound

inferred from mutant phenotype

(Gong et al., 2004)

inferred from mutant phenotype

(Kim et al., 2003)

Cellular Component

CV term

Evidence

References

membrane

inferred from electronic annotation with InterPro:IPR005821

(FlyBase Curators et al., 2004-)

Sequence Ontology: Class of Gene

protein_coding_gene

nuclear_gene

Interactions & Pathways

Summary of Genetic Interactions

Interacts with

Please look at the allele data for full details of the genetic interactions

iav allele

Gene

References

External Data

Linkouts

Orthologs

Genome-wide drosophilid orthologs

Curated drosophilid orthologs

Dpse\GA18240

Linkouts

Functional Complementation between Species

Inter-Species Misexpression Data

Produces phenotype in

Produces NO phenotype in

Stocks & Reagents

Stocks Listed in FlyBase ( 5 )

101174

6029

24768

iav³⁶²¹

VDRC

v7126

w¹¹¹⁸; P{GD3260}v7126

v7128

w¹¹¹⁸; P{GD3260}v7128

Genomic Clones ( 2 )

cDNA Clones ( 3 )

Please Note

This section lists cDNAs and ESTs that fall within the genomic extent of the gene model, which may include cDNAs and ESTs of genes within introns, or of overlapping genes. Please see GBrowse for alignment of the cDNAs and ESTs to the gene model.

cDNA Clones, Fully Sequenced

BDGP DGC clones

Other clones

cDNA Clones, End Sequenced (ESTs)

BDGP DGC clones

Other clones

RNAi & Array Information

Affy Oligo

Linkouts

Antibody Information

Other Information

Discoverer

Etymology

Identification

Position Effect Variegation Data

Relationship to Other Genes

Source for database identity of

Source for database merge of

Source for merge of: iav CG4536

(Gong et al., 2004)

Additional comments

Comments About Role

Comments About Molecular Function

Lower than normal octopamine levels, especially in extracts from adult heads with dopamine and norepinephrine levels apparently normal.

(O'Dell et al., 1987)

Other Comments

Allelism of iav¹ to iav^hypoB-1 shown by O'Dell and Burnet (1986) in open field tests of heterozygotes; they also note that both mutations are recessive.

External Crossreferences & Linkouts

Sequence Crossreferences

RefSeq (Transcripts)

•

NM_132125

RefSeq (Proteins)

•

NP_572353

DDBJ / EMBL / GenBank

•

UniProtKB/TrEMBL

•

Q9W3W0

Other Crossreferences

InterPro domains - A database of protein families, domains, and functional sites

•

Ion transport (IPR005821)

Ankyrin (IPR002110)

Linkouts

Synonyms & Secondary IDs ( 6 )

Reported As

Symbol Synonym

CG4536

(Mutai and Heller, 2003, King et al., 2002.9.23, McPartland et al., 2006, Roos et al., 2005)

CT14708

(Littleton and Ganetzky, 2000)

hypoB

iav

(Gong et al., 2004, Lumpkin, 2007, McClung and Hirsh, 1999, Gopfert et al., 2006)

TRPV

(Caterina and Montell, 2005)

Name Synonym

inactive

(Lumpkin, 2007)

Secondary FlyBase IDs

FBgn0029904
FBgn0001246

References ( 50 )

Generate a list of

All references relating to this gene ( 50 )

List References by type

Recent research papers ( 2 )

Gopfert et al., 2006, Nature Neurosci. 9(8): 999--1000: Specification of auditory sensitivity by Drosophila TRP channels. [FBrf0194313]
McPartland et al., 2006, Gene 370: 64--74: Evolutionary origins of the endocannabinoid system. [FBrf0190501]

Show all research papers ...

Recent reviews ( 1 )

Lumpkin, 2007, Nature 445(7130): 858--865: [FBrf0200026]

Show all reviews ...