Gene Dmel\Hsp68

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General Information

Symbol

Dmel\Hsp68

Species

D. melanogaster

Name

Heat shock protein 68

Annotation symbol

CG5436

Feature type

protein_coding_gene

FlyBase ID

FBgn0001230

Created / Updated

2003-12-01/2003-12-01

Genomic Location

Chromosome (arm)

Recombination map

Cytogenetic map

95D11-95D11

Sequence location

3R:19,880,802..19,883,029 [-]

Map ( GBrowse )

Summary Information

Automatically generated summary

See sections below for more information

The gene Heat shock protein 68 is referred to in FlyBase by the symbol Hsp68 (CG5436, FBgn0001230). It has the cytological map location 95D11. Its sequence location is 3R:19880802..19883029. Its molecular function is described as: unfolded protein binding; ATP binding. It is involved in the biological processes: determination of adult life span; response to heat; protein folding. 8 alleles are reported. No phenotypic data is available. It has one annotated transcript and one annotated polypeptide.

Phenotypic Description from the Red Book (Lindsley & Zimm 1992)

Gene/Allele symbols may differ from current usage

Hsp68

The structural gene for the 68,000 dalton heat-shock protein (HSP68).

Detailed Mapping Data

FlyBase Computed Cytological Location

Cytogenetic map

Evidence for location

95D11-95D11

Limits computationally determined from genome sequence between P{PZ}Atg6⁰⁰⁰⁹⁶ and P{PZ}Syx1A⁰⁶⁷³⁷&P{PZ}Syx1A¹⁰⁶⁶⁰

Experimentally Determined Cytological Location

Cytogenetic map

Notes

References

95D5-95D9

(McColl, 1998.10.2)

95D-95D

(determined by in situ hybridisation)

(Holmgren et al., 1979)

Experimentally Determined Recombination Data

Location

Left of (cM)

Right of (cM)

Notes

Molecular Map Data

Gene Order (in direction of increasing cytology)

References

Gene Order (overall orientation not stated)

References

Overall orientation not stated: Hsp68+ Rox8+

(Brand and Bourbon, 1993)

Gene Model & Products

Please see the GBrowse view of Dmel\Hsp68 for information on other features

Comments on Gene Model

Transcript Data

Annotated Transcripts

Name

FlyBase ID

RefSeq ID

Length (nt)

Associated CDS (aa)

Hsp68-RA

FBtr0084589

NM_079750

2228

635

Additional Transcript Data & Comments

Reported size (kB)

2.4 (northern blot)

(Holmgren et al., 1979)

Comments

External Data

Crossreferences

Polypeptide Data

Annotated Polypeptides

Name

FlyBase ID

Predicted MW (kD)

Length (aa)

Theoretical pI

RefSeq ID

GenBank protein

Hsp68-PA

FBpp0083974

69.7

635

5.54

NP_524474

AAF56230

Additional Polypeptide Data & Comments

Reported size (kD)

68 (kD)

(Holmgren et al., 1979)

Comments

External Data

Linkouts

PANTHER - Protein classification by function, families, and pathways

•

FBgn0001230

Crossreferences

InterPro domains - A database of protein families, domains, and functional sites

•

Heat shock protein Hsp70 (IPR001023)

Heat shock protein 70 (IPR013126)

MitoDrome - A database of annotated Dmel nuclear genes encoding mitochondrial proteins

•

MTDROME05436

Sequences Consistent with the Gene Model

DDBJ /
EMBL /
GenBank

DNA sequence

Protein sequence

Name

O97125

UniProtKB/TrEMBL

Maps to

Does NOT map to

Identified with

RE48592

(BDGP Project Members, 2000-)

Mapped Features & Mutations

Please see GBrowse or insertion reports for information on insertions of transgenic constructs and features not listed here

Type

Symbol & Location

Additional Notes

References

External Data

Linkouts

DEDB - Drosophila exon database: splicing graphs

•

10586

Crossreferences

EPD - Eukarytoic Promoter Database, an annotated collection of POL II promoters

•

07031

Expression Data

FlyBase-Curated Data

Transcript and
Protein data

Please see the FlyBase Gene Expression Report for details of gene expression from the literature.

Summary of Transcript Expression

Stage

Tissue/Position

Reference

Marker for

Subcellular Localization

CV Term

Summary of Polypeptide Expression

Stage

Tissue/Position

Reference

Marker for

Subcellular Localization

CV Term

External Data & Images

Linkouts

FLIGHT - Cell culture data for RNAi and other high-throughput technologies

•

FBgn0001230

FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array

•

FBgn0001230

GEO (NCBI) - Gene expression data: microarray and other high-throughput technologies

•

FBgn0001230

Alleles & Phenotypes

Summary of Allele Phenotypes

Other Phenotypes

Allele

long lived, with Scer\GAL4^arm.PS

Hsp68^Scer\UAS.cWa

chemical resistant, with Scer\GAL4^arm.PS

Hsp68^Scer\UAS.cWa

Phenotype manifest in

Allele

Classical Alleles ( 5 )

For All Classical Alleles Show

Allele of Hsp68	Mutagen	Stocks	Known lesion
Hsp68^DG16802	P-element activity	1	--
Hsp68^a	natural population	0	--
Hsp68^b	natural population	0	--
Hsp68^d	natural population	0	--
Hsp68^f	natural population	0	--

Alleles Carried on Transgenic Constructs ( 3 )

For All Alleles Carried on Transgenic Constructs Show

Allele of Hsp68	Mutagen	Stocks	Known lesion
Hsp68^GD11651	in vitro construct \| RNAi in vitro construct \| regulatory fusion	1	Yes
Hsp68^GD16361	in vitro construct \| RNAi in vitro construct \| regulatory fusion	2	Yes
Hsp68^Scer\UAS.cWa	in vitro construct \| regulatory fusion	0	Yes

Aneuploid Aberrations

Useful deficiency

Df(3R)Su9

Useful duplication

Dp(3;3)M95A⁺13

Disrupted in

Df(3R)Exel6197

(Ryder, 2004.4.26)

Transgenic Constructs & Insertions

Transgenic Constructs

Type of construct

Name

Expression data

UAS construct

P{GD11651}

P{GD16361}

P{UAS-Hsp68.W}

Insertions

Type of insertions

Name

Expression data

miscellaneous insertions

P{wHy}Hsp68^DG16802

insertion of mobile activating element

P{EPgy2}EY00050

Related Comments

Please look at the allele reports for the complete phenotype data

The structural gene for the 68,000 dalton heat-shock protein (HSP68)

Probes from D.melanogaster used in chromosome in situ hybridisation to study response to heat shock in D.guanche, D.madeirensis and D.subobscura. Results suggest that the 18C, 94A, 89A and 27A loci of the three obscura group species are homologous to the D.melanogaster loci Hsp83, Hsp70A, Hsp68 and the small Hsp group Hsp22, Hsp23, Hsp26 and Hsp27 respectively.

(Molto et al., 1992)

Members of the hsc70 gene family (heat shock cognate genes) that reside within the same intracellular compartment in different organisms share greater amino acid identity than hsc70 proteins from the same organism but different organelles. This pattern of conservation indicates specialisation of hsc70 function.

(Rubin et al., 1993)

Synthesis of heat shock proteins is inhibited by both short-chain fatty acids and their corresponding alcohols, compounds which have no observable effect on histone acetylation.

(Munks and Turner, 1994)

Gene Ontology: Function, Process & Cellular Component ( 7 )

Molecular Function

CV term

Evidence

References

ATP binding

inferred from electronic annotation with InterPro:IPR001023

(FlyBase Curators et al., 2004-)

unfolded protein binding

inferred from sequence or structural similarity with UniProtKB:P38646

(Sardiello et al., 2003.6.11)

Biological Process

CV term

Evidence

References

determination of adult life span

inferred from mutant phenotype

(Wang et al., 2003)

protein folding

inferred from sequence or structural similarity with UniProtKB:P38646

(Sardiello et al., 2003.6.11)

response to heat

inferred from sequence or structural similarity with MGD:Hspa1b; MGI:MGI:99517

(FlyBase, 1992-)

non-traceable author statement

(Swiss-Prot Project Members, 2000.10.1)

Cellular Component

CV term

Evidence

References

lipid particle

inferred from direct assay

(Beller et al., 2006)

mitochondrion

inferred from sequence or structural similarity with UniProtKB:P38646

(Sardiello et al., 2003.6.11)

Sequence Ontology: Class of Gene

protein_coding_gene

nuclear_gene

Interactions & Pathways

Summary of Genetic Interactions

Interacts with

Please look at the allele data for full details of the genetic interactions

Hsp68 allele

Gene

References

External Data

Linkouts

BioGRID - Interaction data, including yeast 2-hybrid and genetic interactions

•

67786

Orthologs

Genome-wide drosophilid orthologs

Dana\GF17965

Dere\Hsp68

Dgri\GH19098

Dmoj\GI24163

Dper\GL24150

Dpse\GA18881

Dsec\Hsp68

Dsim\Hsp68

Dvir\GJ14196

Dyak\Hsp68

Curated drosophilid orthologs

Damb\Hsp68

Daur\Hsp68

Dbio\Hsp68

Dbir\Hsp68

Dbun\Hsp68

Ddip\Hsp68

Dere\Hsp68

(Kellett, 2000.3.22)

Dgua\Hsp68

Djam\Hsp68

Dkik\Hsp68

Dlum\Hsp68

Dmad\Hsp68

Dmau\Hsp68

(Kellett, 2000.3.22)

Dpse\GA18881

Dqua\Hsp68

Dsec\Hsp68

Dseg\Hsp68

Dser\Hsp68

Dsim\Hsp68

Dsub\Hsp68

Dtei\Hsp68

Dtra\Hsp68

Dvir\Hsp68

(Ranz et al., 1999)

Dvul\Hsp68

Dyak\Hsp68

(Kellett, 2000.6.14)

Linkouts

Functional Complementation between Species

Inter-Species Misexpression Data

Produces phenotype in

Produces NO phenotype in

Stocks & Reagents

Stocks Listed in FlyBase ( 5 )

VDRC

v47146

w¹¹¹⁸ P{GD16361}v47146

v47145

w¹¹¹⁸; P{GD16361}v47145

v35007

w¹¹¹⁸; P{GD11651}v35007/CyO

Bloomington

14806

y¹ w^67c23; P{EPgy2}EY00050

21554

y¹ w^67c23; P{wHy}Hsp68^DG16802 CG5986^DG16802

Genomic Clones ( 1 )

BACR15J11

cDNA Clones ( 44 )

Please Note

This section lists cDNAs and ESTs that fall within the genomic extent of the gene model, which may include cDNAs and ESTs of genes within introns, or of overlapping genes. Please see GBrowse for alignment of the cDNAs and ESTs to the gene model.

cDNA Clones, Fully Sequenced

BDGP DGC clones

RE48592

Other clones

cDNA Clones, End Sequenced (ESTs)

BDGP DGC clones

Other clones

RNAi & Array Information

Affy Oligo

Linkouts

DRSC - RNAi screening (Harvard): high-throughput cell culture data and design

•

FBgn0001230

GenomeRNAi - RNAi phenotypes (Heidelberg): high-throughput cell culture data

•

FBgn0001230

Antibody Information

Other Information

Discoverer

Etymology

Identification

Position Effect Variegation Data

Relationship to Other Genes

Source for database identity of

Source for identity of: Hsp68 CG5436

(Kodira, 1999.11)

Source for database merge of

Additional comments

Comments About Role

Nascent chain nuclear run-on assays in KC161 cells reveal different responses to heat shock for different genes. Transcription of His1 is severely inhibited under mild heat shocks, of Act5C decreases proportionally with increasing temperature while that of the core histone genes or the heat shock cognates is repressed only under extreme heat shock. In unshocked cells Hsp83 is moderately transcribed while transcription from the other heat shock genes is undetectable. Engaged but paused RNA molecules are found at the various Hsp70 and Hsp26 genes but not at the other heat shock genes. Increased transcription of the heat shock genes is observed within 1-2 mins of heat shock and maximal rates were reached within 2-5 minutes. Rates of transcription vary over a 20-fold range. Hsrω is transcribed at a very high rate under non-heat shock conditions, and its response to elevated temperatures is different from that of the protein coding heat shock genes.

(Vazquez et al., 1993)

Comments About Molecular Function

Other Comments

Exposure of cells to pulses of elevated temperature initiates the heat-shock response. A restricted subset of genes, the Hsp genes, is activated and the majority of transcription and translation is shut down. However, mitochondrial- and histone-gene activities persist (Spradling et al., 1977). This response follows a pulse of 36^oC to 40^oC; treatments above 40^oC inhibit all activity and lead to death; treatments of 30^oC-35^oC induce heat-shock-protein synthesis without repressing normal protein synthesis (Tissieres, Mitchell and Tracy, 1974). Similar response inducible by other stressful treatments. The response may be elicited at all stages of the life cycle and in cultured cells. Stage specific phenocopies result from heat shocking early stages of Drosophila development (Mitchell and Petersen, 1982). In polytene cells existing puffs regress and a novel group quickly appears at 33B, 63C, 64F, 67B, 70A, 87A, 87C, 93D, 95D (Ashburner, 1970; Tissieres, Mitchell and Tracy, 1974). Activation of transcription of Hsp genes apparently involves the sequential binding of two or more protein factors in vicinity of TATA box (Wu, 1984). Binding sites for these proteins are multiple short upstream sequence elements called HSEs or heat shock consensus elements (Pelham, 1982; Xiao and Lis, 1988). Polymerase II dissociates from most chromosome regions and accumulates at the new puff sites (Bonner and Kerby, 1982). 3H-uridine incorporation ceases at its usual positions and commences at new puff sites. Preexisting polysomes disaggregate and within a few minutes a new population of polysomes appears containing newly transcribed mRNA; this RNA hybridizes to some of the heat-shock puffs. The effects of heat shock may be abrogated to some degree by pretreatment with a pulse of a slightly lower temperature (Mitchell, Moller, Petersen and Lipps-Sarmiento, 1979; Peterson and Mitchell, 1981). For reviews of the heat-shock response see Ashburner and Bonner (1978).

Area matching Drosophila HSP68 gene (inverted), Acc. No. J01102.

(Davies, 2001.3.15)

Homologous genetic loci in D.subobscura and D.melanogaster tend to show a similar ultrastructure in the two species.

(Cuenca et al., 1998)

External Crossreferences & Linkouts

Sequence Crossreferences

RefSeq (Transcripts)

•