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Gene Dmel\Hsp67Bb

General Information
Symbol	Dmel\Hsp67Bb	Species	D. melanogaster
Name	Heat shock gene 67Bb	Annotation symbol	CG4456
Feature type	protein_coding_gene	FlyBase ID	FBgn0001228
Created / Updated	2005-03-17/2005-03-17
Genomic Location
Chromosome (arm)	3L	Recombination map
Cytogenetic map	67B2-67B2	Sequence location	3L:9,366,031..9,368,070 [+]
Map ( GBrowse )	Decorated FastA GenBankGFF Gene region Extended Gene region CDSIntronsExonsTranslationsTranscripts3' UTR5' UTR
Summary Information
Automatically generated summary See sections below for more information	The gene Heat shock gene 67Bb is referred to in FlyBase by the symbol Hsp67Bb (CG4456, FBgn0001228). It has the cytological map location 67B2. Its sequence location is 3L:9366031..9368070. Its molecular function is unknown. It is involved in the biological process response to heat. 3 alleles are reported. No phenotypic data is available. It has 2 annotated transcripts and 2 annotated polypeptides.
Phenotypic Description from the Red Book (Lindsley & Zimm 1992)
Gene/Allele symbols may differ from current usage	Hsp22 - Hsp-G3 There are seven closely related heat-shock genes at 67B (Ayme and Tissieres, 1985; Pauli, Arrigo, Vasquez, Tonka, and Tissieres, 1989, Genome 31: 671-76). In addition to the four small heat-shock genes previously identified (Hsp22, Hsp23, Hsp26, and Hsp27), three more genes (Hsp-G1, Hsp-G2, and Hsp-G3, formerly called Gene1, Gene2, and Gene3) have been found clustered within 15 kb of DNA at the same 67B cytological location. All seven genes are heat-shock inducible in almost all cells at the stages tested (Ayme and Tissieres, 1985). The genes are also transcribed during certain developmental stages in the absence of heat shock (Sirotkin and Davidson, 1982, Dev. Biol. 89: 196-210). Pauli et al (1989) report that the maximum accumulation of developmental rRNA in a majority of these small heat-shock genes occurs in the white pupae stage; in Hsp-G2, however, a small transcipt is found in embryos, first and second instar larvae, and young pupae; and a larger transcript in the pupal and adult stages of males (Pauli and Tonka, 1987, J. Mol. Biol. 198: 235-40; Pauli, Tonka, and Ayme-Southgate, 1988, J. Mol. Biol. 200: 47-53). In absence of stress, the expression of Hsp26 has been observed in spermatocytes, nurse cells, epithelium, imaginal discs, proventriculus, and neurocytes (Glaser, Wolfner, and Lis, 1986, EMBO 5: 747-54). Transcripts of Hsp26 and Hsp27 accumulate in adult ovaries, apparently originating in nurse cells (Zimmerman, Petri, and Meselson, 1983, Cell 32: 1161-70).
Detailed Mapping Data
FlyBase Computed Cytological Location
Cytogenetic map Evidence for location 67B2-67B2   Limits computationally determined from genome sequence between P{PZ}l(3)0162901629&P{PZ}mRpL1210534 and P{EP}Hsp26EP3336&P{EP}Hsp26EP3315
Experimentally Determined Cytological Location
Cytogenetic map Notes References
Experimentally Determined Recombination Data
Location
Left of (cM)
Right of (cM)
Notes
Molecular Map Data
Gene Order (in direction of increasing cytology) References Gene Order (overall orientation not stated) References Overall orientation not stated: Hsp27- Hsp23- Hsp67Ba+ Hsp26+ Hsp22- Hsp67Bb- Hsp67Bc+ (Pauli and Tonka, 1987, Pauli et al., 1989)
Gene Model & Products
Please see the GBrowse view of Dmel\Hsp67Bb for information on other features
Comments on Gene Model
	gene_with_dicistronic_processed_transcript ; SO:0000722
Transcript Data
Annotated Transcripts
Name FlyBase ID RefSeq ID Length (nt) Associated CDS (aa) Hsp67Bb-RA FBtr0100653  NM_001031946   585   111 Hsp67Bb-RB FBtr0100655  NM_001031945   1906   111
Additional Transcript Data & Comments
Reported size (kB)	2, 0.78, 0.56 (northern blot) (Pauli et al., 1988)
Comments	Upon heat shock, a 2.0 kb message is observed, which includes not only the complete Hsp67Bb transcript, but also the complete downstream Hsp22 ORF. (Pauli et al., 1988)
External Data
Crossreferences
Polypeptide Data
Annotated Polypeptides
Name FlyBase ID Predicted MW (kD) Length (aa) Theoretical pI RefSeq ID GenBank protein Hsp67Bb-PA   FBpp0100114   12.6   111   8.43     NP_001027117   AAN11961 Hsp67Bb-PB   FBpp0100115   12.6   111   8.43     NP_001027116   AAF50291
Additional Polypeptide Data & Comments
Reported size (kD)	111 (aa) (Pauli et al., 1988)
Comments
External Data
Linkouts
Crossreferences	InterPro domains - A database of protein families, domains, and functional sites • Rhodanese-like (IPR001763)
Sequences Consistent with the Gene Model
DDBJ / EMBL / GenBank	DNA sequence Protein sequence Name AA536253   AA536255   AE003552 AAF50291   AE003552 AAN11961   AI456272   AI520460   AQ073854   AW943326   AX093926   AX154789   AY119034   BE978184   BG638118   BP550847   BP551679   BP551680   CL610262   CO156081   CO182502   CO185064   CO300605   EC085636   EC201226   EC264820   LD16746   X07311 CAA30276
UniProtKB/Swiss-Prot	P22978
UniProtKB/TrEMBL
Maps to
Does NOT map to
Identified with	LD36162 (BDGP Project Members, 2000-)
Mapped Features & Mutations
Please see GBrowse or insertion reports for information on insertions of transgenic constructs and features not listed here Type Symbol & Location Additional Notes References
External Data
Linkouts
Crossreferences
Expression Data
FlyBase-Curated Data
Transcript and Protein data	Please see the FlyBase Gene Expression Report for details of gene expression from the literature.
Summary of Transcript Expression
Stage Tissue/Position Reference  larval stage | second instar   (Pauli et al., 1988)  larval stage | third instar   (Pauli et al., 1988)  adult stage | male   (Pauli et al., 1988)  pupal stage | early   (Pauli et al., 1988)  adult stage   (Pauli et al., 1988)  adult stage,spermatogenesis  testis (Pauli et al., 1988)  embryonic stage | >=7 hr   (Pauli et al., 1988)  larval stage | first instar   (Pauli et al., 1988)  pupal stage   (Pauli et al., 1988)
Marker for
Subcellular Localization
CV Term
Summary of Polypeptide Expression
Stage Tissue/Position Reference
Marker for
Subcellular Localization
CV Term
External Data & Images
Linkouts	FLIGHT - Cell culture data for RNAi and other high-throughput technologies • FBgn0001228 GEO (NCBI) - Gene expression data: microarray and other high-throughput technologies • FBgn0001228
Alleles & Phenotypes
Summary of Allele Phenotypes
Lethality Allele viable Hsp67BbEY09909 Sterility Allele fertile Hsp67BbEY09909 Phenotype manifest in Allele
Classical Alleles ( 3 )
For All Classical Alleles Show
Allele of Hsp67Bb Class Mutagen Stocks Known lesion Hsp67BbBG02409 1 Yes Hsp67BbEP3247 P-element activity 1 Yes Hsp67BbEY09909 P-element activity 1 Yes
Alleles Carried on Transgenic Constructs ( 0 )
For All Alleles Carried on Transgenic Constructs Show
Allele of Hsp67Bb Class Mutagen Stocks Known lesion
Aneuploid Aberrations
Useful deficiency	Df(3L)AC1
Useful duplication	Dp(3;3)M67C+2
Transgenic Constructs & Insertions
Transgenic Constructs
	Type of construct Name Expression data UAS construct P{GD17696} NA P{GD17697} NA P{GD8704} NA
Insertions
	Type of insertions Name Expression data miscellaneous insertions P{GT1}Hsp22BG02409 NA insertion of mobile activating element P{EPgy2}Hsp22EY09909 NA P{EP}Hsp67BbEP3247 NA
Related Comments
	Please look at the allele reports for the complete phenotype data
Gene Ontology: Function, Process & Cellular Component ( 1 )
Molecular Function
CV term Evidence References
Biological Process
CV term Evidence References
response to heat non-traceable author statement (FlyBase, 1992-)
Cellular Component
CV term Evidence References
Sequence Ontology: Class of Gene
	protein_coding_gene   nuclear_gene   gene_with_dicistronic_mRNA (FlyBase Genome Annotators, 2002-2003)
Interactions & Pathways
Summary of Genetic Interactions
Interacts with	Please look at the allele data for full details of the genetic interactions Hsp67Bb allele Gene References
External Data
Linkouts
Orthologs
Genome-wide drosophilid orthologs	Dana\GF10688 Dere\GG15366 Dgri\GH14585 Dmoj\GI12243 Dper\GL22440 Dpse\GA24291 Dsec\GM25139 Dsim\GD14173 Dvir\GJ11475 Dwil\GK17634 Dyak\Hsp67Bb
Curated drosophilid orthologs	Dyak\Hsp67Bb
Linkouts
Functional Complementation between Species
Inter-Species Misexpression Data
Produces phenotype in
Produces NO phenotype in
Stocks & Reagents
Stocks Listed in FlyBase ( 3 )
Szeged	- P{EP}Hsp67BbEP3247
Bloomington	12683 w1118; P{GT1}Hsp22BG02409 Hsp67BbBG02409 20055 y1 w67c23; P{EPgy2}Hsp22EY09909 Hsp67BbEY09909
Genomic Clones ( 1 )
	BACR01E20
cDNA Clones ( 10 )
	Please Note This section lists cDNAs and ESTs that fall within the genomic extent of the gene model, which may include cDNAs and ESTs of genes within introns, or of overlapping genes. Please see GBrowse for alignment of the cDNAs and ESTs to the gene model.
cDNA Clones, Fully Sequenced
BDGP DGC clones	LP05734 LD36162
Other clones
cDNA Clones, End Sequenced (ESTs)
BDGP DGC clones	LD40634 LD16753
Other clones	EC22858 bs75b08 EC29258 EN07317 EK178661 LD16746
RNAi & Array Information
Affy Oligo
Linkouts	DRSC - RNAi screening (Harvard): high-throughput cell culture data and design • FBgn0001228
Antibody Information
Other Information
Discoverer
Etymology
Identification
Position Effect Variegation Data
Relationship to Other Genes
Source for database identity of
Source for database merge of
Additional comments	gene_with_dicistronic_mRNA (SO:0000722) which encodes Hsp67Bb (FBgn0001228), Hsp22 (FBgn0001223) One or more of the processed transcripts for this gene contain(s) two non-overlapping open reading frames (ORFs). The non-overlapping ORFs are represented by Hsp67Bb (FBgn0001228), Hsp22 (FBgn0001223). (FlyBase Genome Annotators, 2002-2003) Dicistronic annotation CG32041 split out into separate annotations for each open reading frame, CG4456 and CG4460, in release 4.2 of the genome annotation. CG4456 corresponds to Hsp67Bb and CG4460 corresponds to Hsp22. (FlyBase Genome Annotators, 2005)
Comments About Role
	In polytene cells, during heat shock response, existing puffs regress and a novel group quickly appear at cytological locations 33B, 63C, 64F, 67B, 70A, 87A, 87C, 93D, 95D. (Ashburner, 1970) The heat shock response follows a pulse of 36oC to 40oC; treatments above 40oC inhibit all activity and lead to death; treatments of 30oC-35oC induce heat-shock-protein synthesis without repressing normal protein synthesis. (Tissieres et al., 1974)
Comments About Molecular Function
	Heat shock induces the accumulation of a dicistronic mRNA which contains both the Hsp22 and Hsp67Bb open reading frames. Overall orientation not stated: Hsp27- Hsp23- Hsp67Ba+ Hsp26+ Hsp22- Hsp67Bb- Hsp67Bc+ (Pauli et al., 1988) Mitochondrial and histone-gene activities persist transcription and translation. (Spradling et al., 1977) The effects of heat shock may be abrogated to some degree by pretreatment with a pulse of a slightly lower temperature. (Mitchell et al., 1979, Petersen and Mitchell, 1981) The binding sites for the protein factors required for activation of transcription of Hsp genes are multiple short upstream sequence elements called HSEs or heat shock consensus elements. (Pelham, 1982, Xiao and Lis, 1988) Polymerase II dissociates from most chromosome regions and accumulates at the new heat shock puff sites upon heat shock. (Bonner and Kerby, 1982) Hsp67Bb is transcribed during certain developmental stages in the absence of heat shock. (Sirotkin and Davidson, 1982) Activation of transcription of Hsp genes apparently involves the sequential binding of two or more protein factors in vicinity of TATA box. (Wu, 1984) Hsp67Bb shows weak homology to Hsp22. It is heat inducible. (Ayme-Southgate and Tissieres, 1985) In unshocked cells Hsp83 is moderately transcribed while transcription from the other heat shock genes is undetectable. Engaged but paused RNA molecules are found at the various Hsp70 and Hsp26 genes but not at the other heat shock genes. Increased transcription of the heat shock genes is observed within 1-2 mins of heat shock and maximal rates were reached within 2-5 minutes. Rates of transcription vary over a 20-fold range. (Vazquez et al., 1993) Exposure of cells to pulses of elevated temperature initiates the heat-shock response. A restricted subset of genes, the Hsp genes, is activated and the majority of transcription and translation is shut down. 3H-uridine incorporation ceases at its usual positions and commences at new puff sites. Preexisting polysomes disaggregate and within a few minutes a new population of polysomes appears containing newly transcribed mRNA; this RNA hybridizes to some of the heat-shock puffs. Similar response inducible by other stressful treatments. The response may be elicited at all stages of the life cycle and in cultured cells. (Lindsley and Zimm, 1992)
Other Comments
	In polytene cells, during heat shock response, existing puffs regress and a novel group quickly appear at 33B, 63C, 64F, 67B, 70A, 87A, 87C, 93D, 95D. (Tissieres et al., 1974)
External Crossreferences & Linkouts
Sequence Crossreferences
	RefSeq (Transcripts) • NM_001031946 NM_001031945 RefSeq (Proteins) • NP_001027117 NP_001027116 DDBJ / EMBL / GenBank • AAN11961.1 AAN11962.1 AAF50290.1 AAF50291.1 AE003552 AAF50291 AAN11961 AI456272 AQ073854 AW943326 AX093926 AX154789 AY060412 CL610262 X07311 CAA30276 UniProtKB/Swiss-Prot • P22978
Other Crossreferences
	InterPro domains - A database of protein families, domains, and functional sites • Rhodanese-like (IPR001763)
Linkouts
	DRSC - RNAi screening (Harvard): high-throughput cell culture data and design • FBgn0001228 FLIGHT - Cell culture data for RNAi and other high-throughput technologies • FBgn0001228 FlyMine - Integrated genomics database for Drosophila, Anopheles, and C.elegans • FBgn0001228 GEO (NCBI) - Gene expression data: microarray and other high-throughput technologies • FBgn0001228 University of Chicago Dev. Expression • FBgn0001228
Synonyms & Secondary IDs ( 16 )
Reported As
Symbol Synonym	anon-WO0118547.50   anon-WO0140519.138   CG4456   CG32041 (Giot et al., 2003, ) EP(3)3247 (Pena-Rangel et al., 2002) gene2 (Southgate et al., 1985) gene 2 (Vazquez et al., 1993, Vazquez, 1991, Pauli et al., 1989, Pauli et al., 1988, Ayme-Southgate and Tissieres, 1985) gene-2   gene4 (Sirotkin and Davidson, 1982) hsp67B (Ringrose et al., 2003) Hsp67Bb (Yepiskoposyan et al., 2006) Hspg2 (Maroni, 1994) Hsp-G2
Name Synonym	Gene 2 (Pauli and Tonka, 1987) heat shock gene 2. (Pauli, 1997.11.7) Heat shock gene 67Bb
Secondary FlyBase IDs
	FBgn0044743 FBgn0052041 FBgn0061943 FBtr0076448 FBpp0076176 FBtr0076449 FBpp0076177 FBtr0076450 FBpp0076178 FBtr0076451 FBpp0076179
References ( 35 )
Generate a list of	All references relating to this gene ( 35 )
List References by type	Research paper  ( 22 ) Supplementary material  ( 1 ) FlyBase analysis  ( 4 ) DNA/RNA sequence record  ( 1 ) Book  ( 1 ) Patent  ( 2 ) Computer file  ( 2 ) Curated genome annotation  ( 1 ) Automatic genome annotation  ( 1 )
Recent research papers ( 1 )
	Yepiskoposyan et al., 2006, Nucleic Acids Res. 34(17): 4866--4877 Transcriptome response to heavy metal stress in Drosophila reveals a new zinc transporter that confers resistance to zinc. [FBrf0193346] Show all research papers ...