Gene Dmel\Hsp67Ba

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General Information

Symbol

Dmel\Hsp67Ba

Species

D. melanogaster

Name

Heat shock gene 67Ba

Annotation symbol

CG4167

Feature type

protein_coding_gene

FlyBase ID

FBgn0001227

Created / Updated

2003-12-01/2008-06-17

Genomic Location

Chromosome (arm)

Recombination map

Cytogenetic map

67B2-67B2

Sequence location

3L:9,370,902..9,372,634 [-]

Map ( GBrowse )

Summary Information

Automatically generated summary

See sections below for more information

The gene Heat shock gene 67Ba is referred to in FlyBase by the symbol Hsp67Ba (CG4167, FBgn0001227). It has the cytological map location 67B2. Its sequence location is 3L:9370902..9372634. Its molecular function is unknown. It is involved in the biological process response to heat. 2 alleles are reported. No phenotypic data is available. It has one annotated transcript and one annotated polypeptide.

Phenotypic Description from the Red Book (Lindsley & Zimm 1992)

Gene/Allele symbols may differ from current usage

Hsp22 - Hsp-G3

There are seven closely related heat-shock genes at 67B (Ayme and Tissieres, 1985; Pauli, Arrigo, Vasquez, Tonka, and Tissieres, 1989, Genome 31: 671-76). In addition to the four small heat-shock genes previously identified (Hsp22, Hsp23, Hsp26, and Hsp27), three more genes (Hsp-G1, Hsp-G2, and Hsp-G3, formerly called Gene1, Gene2, and Gene3) have been found clustered within 15 kb of DNA at the same 67B cytological location. All seven genes are heat-shock inducible in almost all cells at the stages tested (Ayme and Tissieres, 1985). The genes are also transcribed during certain developmental stages in the absence of heat shock (Sirotkin and Davidson, 1982, Dev. Biol. 89: 196-210). Pauli et al (1989) report that the maximum accumulation of developmental rRNA in a majority of these small heat-shock genes occurs in the white pupae stage; in Hsp-G2, however, a small transcipt is found in embryos, first and second instar larvae, and young pupae; and a larger transcript in the pupal and adult stages of males (Pauli and Tonka, 1987, J. Mol. Biol. 198: 235-40; Pauli, Tonka, and Ayme-Southgate, 1988, J. Mol. Biol. 200: 47-53). In absence of stress, the expression of Hsp26 has been observed in spermatocytes, nurse cells, epithelium, imaginal discs, proventriculus, and neurocytes (Glaser, Wolfner, and Lis, 1986, EMBO 5: 747-54). Transcripts of Hsp26 and Hsp27 accumulate in adult ovaries, apparently originating in nurse cells (Zimmerman, Petri, and Meselson, 1983, Cell 32: 1161-70).

Detailed Mapping Data

FlyBase Computed Cytological Location

Cytogenetic map

Evidence for location

67B2-67B2

Limits computationally determined from genome sequence between P{EP}Hsp26^EP3336&P{EP}Hsp26^EP3315 and P{PZ}fry⁰²²⁴⁰

Experimentally Determined Cytological Location

Cytogenetic map

Notes

References

Experimentally Determined Recombination Data

Location

Left of (cM)

Right of (cM)

Notes

Molecular Map Data

Gene Order (in direction of increasing cytology)

References

Gene Order (overall orientation not stated)

References

Overall orientation not stated: Hsp27- Hsp23- Hsp67Ba+ Hsp26+ Hsp22- Hsp67Bb- Hsp67Bc+

(Pauli and Tonka, 1987, Pauli et al., 1988, Pauli et al., 1989)

Gene Model & Products

Please see the GBrowse view of Dmel\Hsp67Ba for information on other features

Comments on Gene Model

Transcript Data

Annotated Transcripts

Name

FlyBase ID

RefSeq ID

Length (nt)

Associated CDS (aa)

Hsp67Ba-RA

FBtr0076495

NM_079274

1733

445

Additional Transcript Data & Comments

Reported size (kB)

Comments

External Data

Crossreferences

Polypeptide Data

Annotated Polypeptides

Name

FlyBase ID

Predicted MW (kD)

Length (aa)

Theoretical pI

RefSeq ID

GenBank protein

Hsp67Ba-PA

FBpp0076223

46.9

445

4.35

NP_523998

AAF50287

Additional Polypeptide Data & Comments

Reported size (kD)

Comments

External Data

Linkouts

PANTHER - Protein classification by function, families, and pathways

•

FBgn0001227

Crossreferences

InterPro domains - A database of protein families, domains, and functional sites

•

Alpha crystallin/Heat shock protein (IPR001436)

Heat shock protein Hsp20 (IPR002068)

HSP20-like chaperone (IPR008978)

Sequences Consistent with the Gene Model

DDBJ /
EMBL /
GenBank

DNA sequence

Protein sequence

Name

P05812

UniProtKB/TrEMBL

Q5U130

Maps to

Does NOT map to

Identified with

LP13637

(BDGP Project Members, 2000-)

Mapped Features & Mutations

Please see GBrowse or insertion reports for information on insertions of transgenic constructs and features not listed here

Type

Symbol & Location

Additional Notes

References

External Data

Linkouts

DEDB - Drosophila exon database: splicing graphs

•

4656

Crossreferences

Expression Data

FlyBase-Curated Data

Transcript and
Protein data

Please see the FlyBase Gene Expression Report for details of gene expression from the literature.

Summary of Transcript Expression

Stage

Tissue/Position

Reference

prepupal stage

(Pauli et al., 1989)

Marker for

Subcellular Localization

CV Term

Summary of Polypeptide Expression

Stage

Tissue/Position

Reference

Marker for

Subcellular Localization

CV Term

External Data & Images

Linkouts

FLIGHT - Cell culture data for RNAi and other high-throughput technologies

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FBgn0001227

FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array

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FBgn0001227

GEO (NCBI) - Gene expression data: microarray and other high-throughput technologies

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FBgn0001227

Alleles & Phenotypes

Summary of Allele Phenotypes

Phenotype manifest in

Allele

Classical Alleles ( 1 )

For All Classical Alleles Show

Allele of Hsp67Ba	Class	Mutagen	Stocks	Known lesion
Hsp67Ba^C291			1	--

Alleles Carried on Transgenic Constructs ( 1 )

For All Alleles Carried on Transgenic Constructs Show

Allele of Hsp67Ba	Class	Mutagen	Stocks	Known lesion
Hsp67Ba^GD11237		in vitro construct \| RNAi in vitro construct \| regulatory fusion	2	Yes

Aneuploid Aberrations

Useful deficiency

Df(3L)AC1

Useful duplication

Dp(3;3)M67C⁺2

Transgenic Constructs & Insertions

Transgenic Constructs

Type of construct

Name

Expression data

UAS construct

P{GD11237}

Insertions

Type of insertions

Name

Expression data

miscellaneous insertions

PBac{3HPy⁺}Hsp67Ba^C291

Related Comments

Please look at the allele reports for the complete phenotype data

Gene Ontology: Function, Process & Cellular Component ( 1 )

Molecular Function

CV term

Evidence

References

Biological Process

CV term

Evidence

References

response to heat

inferred from sequence or structural similarity with EMBL:U96099

(FlyBase, 1992-)

non-traceable author statement

(Swiss-Prot Project Members, 1988.11.1)

Cellular Component

CV term

Evidence

References

Sequence Ontology: Class of Gene

protein_coding_gene

nuclear_gene

Interactions & Pathways

Summary of Genetic Interactions

Interacts with

Please look at the allele data for full details of the genetic interactions

Hsp67Ba allele

Gene

References

External Data

Linkouts

BioGRID - Interaction data, including yeast 2-hybrid and genetic interactions

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64471

Orthologs

Genome-wide drosophilid orthologs

Dana\GF23738

Dere\GG14046

Dgri\GH14699

Dmoj\GI12246

Dper\GL22631

Dpse\GA18002

Dsec\GM24880

Dsim\GD12932

Dvir\GJ11478

Dwil\GK16565

Dyak\GE21249

Curated drosophilid orthologs

Dpse\GA18002

Linkouts

InParanoid orthologs - Eukaryotic orthologs

•

WBGene00042140
Caenorhabditis briggsae (Wormbase)

Functional Complementation between Species

Inter-Species Misexpression Data

Produces phenotype in

Produces NO phenotype in

Stocks & Reagents

Stocks Listed in FlyBase ( 3 )

VDRC

v21806

w¹¹¹⁸; P{GD11237}v21806

v21807

w¹¹¹⁸; P{GD11237}v21807

Bloomington

16329

y¹ w¹¹¹⁸; PBac{3HPy⁺}Hsp67Ba^C291

Genomic Clones ( 1 )

BACR01E20

cDNA Clones ( 4 )

Please Note

This section lists cDNAs and ESTs that fall within the genomic extent of the gene model, which may include cDNAs and ESTs of genes within introns, or of overlapping genes. Please see GBrowse for alignment of the cDNAs and ESTs to the gene model.

cDNA Clones, Fully Sequenced

BDGP DGC clones

LP13637

Other clones

cDNA Clones, End Sequenced (ESTs)

BDGP DGC clones

Other clones

RNAi & Array Information

Affy Oligo

Linkouts

DRSC - RNAi screening (Harvard): high-throughput cell culture data and design

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FBgn0001227

GenomeRNAi - RNAi phenotypes (Heidelberg): high-throughput cell culture data

•

FBgn0001227

Antibody Information

Other Information

Discoverer

Etymology

Identification

Position Effect Variegation Data

Relationship to Other Genes

Source for database identity of

Source for database merge of

Additional comments

Comments About Role

In polytene cells, during heat shock response, existing puffs regress and a novel group quickly appear at cytological locations 33B, 63C, 64F, 67B, 70A, 87A, 87C, 93D, 95D.

(Ashburner, 1970)

The heat shock response follows a pulse of 36^oC to 40^oC; treatments above 40^oC inhibit all activity and lead to death; treatments of 30^oC-35^oC induce heat-shock-protein synthesis without repressing normal protein synthesis.

(Tissieres et al., 1974)

Comments About Molecular Function

Mitochondrial and histone-gene activities persist transcription and translation.

(Spradling et al., 1977)

The effects of heat shock may be abrogated to some degree by pretreatment with a pulse of a slightly lower temperature.

(Mitchell et al., 1979, Petersen and Mitchell, 1981)

The binding sites for the protein factors required for activation of transcription of Hsp genes are multiple short upstream sequence elements called HSEs or heat shock consensus elements.

(Pelham, 1982, Xiao and Lis, 1988)

Polymerase II dissociates from most chromosome regions and accumulates at the new heat shock puff sites upon heat shock.

(Bonner and Kerby, 1982)

Hsp67Ba is transcribed during certain developmental stages in the absence of heat shock.

(Sirotkin and Davidson, 1982)

Activation of transcription of Hsp genes apparently involves the sequential binding of two or more protein factors in vicinity of TATA box.

(Wu, 1984)

Hsp67Ba has been sequenced, and shows homology to the small heat shock genes Hsp22, Hsp23, Hsp26 and Hsp27. It is heat inducible.

(Ayme-Southgate and Tissieres, 1985)

In contrast to other heat shock genes, the response of Hsp67Ba to stress is modulated during development.

(Vazquez, 1991)

In unshocked cells Hsp83 is moderately transcribed while transcription from the other heat shock genes is undetectable. Engaged but paused RNA molecules are found at the various Hsp70 and Hsp26 genes but not at the other heat shock genes. Increased transcription of the heat shock genes is observed within 1-2 mins of heat shock and maximal rates were reached within 2-5 minutes. Rates of transcription vary over a 20-fold range.

(Vazquez et al., 1993)

Exposure of cells to pulses of elevated temperature initiates the heat-shock response. A restricted subset of genes, the Hsp genes, is activated and the majority of transcription and translation is shut down. ³H-uridine incorporation ceases at its usual positions and commences at new puff sites. Preexisting polysomes disaggregate and within a few minutes a new population of polysomes appears containing newly transcribed mRNA; this RNA hybridizes to some of the heat-shock puffs. Similar response inducible by other stressful treatments. The response may be elicited at all stages of the life cycle and in cultured cells.

(Lindsley and Zimm, 1992)

Other Comments

In polytene cells, during heat shock response, existing puffs regress and a novel group quickly appear at 33B, 63C, 64F, 67B, 70A, 87A, 87C, 93D, 95D.

(Tissieres et al., 1974)

External Crossreferences & Linkouts

Sequence Crossreferences

RefSeq (Transcripts)

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