Gene Dmel\Hsc70-2

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General Information

Symbol

Dmel\Hsc70-2

Species

D. melanogaster

Name

Heat shock protein cognate 2

Annotation symbol

CG7756

Feature type

protein_coding_gene

FlyBase ID

FBgn0001217

Created / Updated

2003-12-01/2008-06-23

Genomic Location

Chromosome (arm)

Recombination map

Cytogenetic map

87D10-87D10

Sequence location

3R:8,870,481..8,873,112 [+]

Map ( GBrowse )

Summary Information

Automatically generated summary

See sections below for more information

The gene Heat shock protein cognate 2 is referred to in FlyBase by the symbol Hsc70-2 (CG7756, FBgn0001217). It has the cytological map location 87D10. Its sequence location is 3R:8870481..8873112. Its molecular function is described as: unfolded protein binding; ATP binding. It is involved in the biological processes: protein folding; response to heat. 4 alleles are reported. No phenotypic data is available. It has one annotated transcript and one annotated polypeptide.

Detailed Mapping Data

FlyBase Computed Cytological Location

Cytogenetic map

Evidence for location

87D10-87D10

Limits computationally determined from genome sequence between P{PZ}CtBP⁰³⁴⁶³ and P{lacW}B52^s2249&P{lacW}flfl^L4179

Experimentally Determined Cytological Location

Cytogenetic map

Notes

References

Experimentally Determined Recombination Data

Location

Left of (cM)

Right of (cM)

Notes

Molecular Map Data

Gene Order (in direction of increasing cytology)

References

In direction of increasing cytology: ry+ snk- Hsc70-2+

(DeLotto and Spierer, 1986)

Gene Order (overall orientation not stated)

References

Gene Model & Products

Please see the GBrowse view of Dmel\Hsc70-2 for information on other features

Comments on Gene Model

15 ESTs indicate a 17 base pair intron within the first exon. However, the predicted splice site does not conform to typical intron/exon junctions. Possible polymorphic site between sequenced strain and the EST libraries.

Transcript Data

Annotated Transcripts

Name

FlyBase ID

RefSeq ID

Length (nt)

Associated CDS (aa)

Hsc70-2-RA

FBtr0082707

NM_079615

2176

633

Additional Transcript Data & Comments

Reported size (kB)

Comments

External Data

Crossreferences

Polypeptide Data

Annotated Polypeptides

Name

FlyBase ID

Predicted MW (kD)

Length (aa)

Theoretical pI

RefSeq ID

GenBank protein

Hsc70-2-PA

FBpp0082175

69.7

633

7.20

NP_524339

AAF54899

Additional Polypeptide Data & Comments

Reported size (kD)

Comments

External Data

Linkouts

PANTHER - Protein classification by function, families, and pathways

•

FBgn0001217

Crossreferences

InterPro domains - A database of protein families, domains, and functional sites

•

Heat shock protein Hsp70 (IPR001023)

Heat shock protein 70 (IPR013126)

MitoDrome - A database of annotated Dmel nuclear genes encoding mitochondrial proteins

•

MTDROME07756

Sequences Consistent with the Gene Model

DDBJ /
EMBL /
GenBank

DNA sequence

Protein sequence

Name

P11146

UniProtKB/TrEMBL

Q5U0V1

Maps to

Does NOT map to

Identified with

AT28983

(BDGP Project Members, 2000-)

(Oliver, 2001.8.16)

Mapped Features & Mutations

Please see GBrowse or insertion reports for information on insertions of transgenic constructs and features not listed here

Type

Symbol & Location

Additional Notes

References

External Data

Linkouts

DEDB - Drosophila exon database: splicing graphs

•

9245

Crossreferences

Expression Data

FlyBase-Curated Data

Transcript and
Protein data

Please see the FlyBase Gene Expression Report for details of gene expression from the literature.

Summary of Transcript Expression

Stage

Tissue/Position

Reference

Marker for

Subcellular Localization

CV Term

Summary of Polypeptide Expression

Stage

Tissue/Position

Reference

Marker for

Subcellular Localization

CV Term

External Data & Images

Linkouts

FLIGHT - Cell culture data for RNAi and other high-throughput technologies

•

FBgn0001217

GEO (NCBI) - Gene expression data: microarray and other high-throughput technologies

•

FBgn0001217

Alleles & Phenotypes

Summary of Allele Phenotypes

Phenotype manifest in

Allele

Classical Alleles ( 1 )

For All Classical Alleles Show

Allele of Hsc70-2	Class	Mutagen	Stocks	Known lesion
Hsc70-2^MB04273			1	--

Alleles Carried on Transgenic Constructs ( 3 )

For All Alleles Carried on Transgenic Constructs Show

Allele of Hsc70-2	Mutagen	Stocks	Known lesion
Hsc70-2^GD16817	in vitro construct \| RNAi in vitro construct \| regulatory fusion	1	Yes
Hsc70-2^GD8848	in vitro construct \| RNAi in vitro construct \| regulatory fusion	1	Yes
Hsc70-2^tDa	in vitro construct \| other	0	Yes

Aneuploid Aberrations

Useful deficiency

Df(3R)ry75

Disrupted in

Df(3R)Exel8157

(Ryder, 2004.4.26)

Df(3R)l26d

(DeLotto and Spierer, 1986)

Not disrupted in

Df(3R)LG27

(DeLotto and Spierer, 1986)

Df(3R)ry36

(DeLotto and Spierer, 1986)

Transgenic Constructs & Insertions

Transgenic Constructs

Type of construct

Name

Expression data

UAS construct

P{GD16817}

P{GD8848}

characterization construct

P{snk⁺13.5}

Insertions

Type of insertions

Name

Expression data

insertion of enhancer trap binary system

Mi{ET1}Hsc70-2^MB04273

Related Comments

Please look at the allele reports for the complete phenotype data

First recognised by sequence similarity to Hsp70. Transcription is developmentally regulated, but is temperature independent.

Members of the hsc70 gene family (heat shock cognate genes) that reside within the same intracellular compartment in different organisms share greater amino acid identity than hsc70 proteins from the same organism but different organelles. This pattern of conservation indicates specialisation of hsc70 function.

(Rubin et al., 1993)

Gene Ontology: Function, Process & Cellular Component ( 5 )

Molecular Function

CV term

Evidence

References

ATP binding

inferred from electronic annotation with InterPro:IPR001023

(FlyBase Curators et al., 2004-)

unfolded protein binding

inferred from sequence or structural similarity with UniProtKB:P38646

(Sardiello et al., 2003.6.11)

Biological Process

CV term

Evidence

References

protein folding

inferred from sequence or structural similarity with MGD:Hspa8; MGI:MGI:105384

(FlyBase, 1992-)

inferred from sequence or structural similarity with UniProtKB:P38646

(Sardiello et al., 2003.6.11)

response to heat

non-traceable author statement

(Swiss-Prot Project Members, 1989.7.1)

Cellular Component

CV term

Evidence

References

mitochondrion

inferred from sequence or structural similarity with UniProtKB:P38646

(Sardiello et al., 2003.6.11)

Sequence Ontology: Class of Gene

protein_coding_gene

nuclear_gene

Interactions & Pathways

Summary of Genetic Interactions

Interacts with

Please look at the allele data for full details of the genetic interactions

Hsc70-2 allele

Gene

References

External Data

Linkouts

BioGRID - Interaction data, including yeast 2-hybrid and genetic interactions

•

66696

Orthologs

Genome-wide drosophilid orthologs

Dana\GF17572

Dere\GG19412

Dgri\GH18372

Dmoj\GI23362

Dpse\GA20564

Dsec\GM24082

Dsim\GD18878

Dvir\GJ10355

Dwil\GK12230

Dyak\GE26242

Curated drosophilid orthologs

Dpse\GA20564

Linkouts

Functional Complementation between Species

Inter-Species Misexpression Data

Produces phenotype in

Produces NO phenotype in

Stocks & Reagents

Stocks Listed in FlyBase ( 3 )

VDRC

v48059

w¹¹¹⁸ P{GD16817}v48059

v19202

w¹¹¹⁸; P{GD8848}v19202/TM3

Bloomington

24686

w¹¹¹⁸; Mi{ET1}Hsc70-2^MB04273

Genomic Clones ( 1 )

BACR48D15

cDNA Clones ( 29 )

Please Note

This section lists cDNAs and ESTs that fall within the genomic extent of the gene model, which may include cDNAs and ESTs of genes within introns, or of overlapping genes. Please see GBrowse for alignment of the cDNAs and ESTs to the gene model.

cDNA Clones, Fully Sequenced

BDGP DGC clones

AT28983

Other clones

cDNA Clones, End Sequenced (ESTs)

BDGP DGC clones

Other clones

RNAi & Array Information

Affy Oligo

Linkouts

DRSC - RNAi screening (Harvard): high-throughput cell culture data and design

•

FBgn0001217

GenomeRNAi - RNAi phenotypes (Heidelberg): high-throughput cell culture data

•

FBgn0001217

Antibody Information

Other Information

Discoverer

Etymology

Identification

Position Effect Variegation Data

Relationship to Other Genes

Source for database identity of

Source for database merge of

Additional comments

Comments About Role

Comments About Molecular Function

Other Comments

transcript abundance embryo: \- transcript abundance larva: \- transcript abundance adult: \+ the codon specifying amino acid 55 is also interrupted by an insertion (Craig et al., 1983).

External Crossreferences & Linkouts

Sequence Crossreferences

RefSeq (Transcripts)

•