Gene Dmel\Hsc70-1

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General Information

Symbol

Dmel\Hsc70-1

Species

D. melanogaster

Name

Heat shock protein cognate 1

Annotation symbol

CG8937

Feature type

protein_coding_gene

FlyBase ID

FBgn0001216

Created / Updated

2003-12-01/2003-12-01

Genomic Location

Chromosome (arm)

Recombination map

Cytogenetic map

70C9-70C9

Sequence location

3L:13,967,277..13,970,879 [+]

Map ( GBrowse )

Summary Information

Automatically generated summary

See sections below for more information

The gene Heat shock protein cognate 1 is referred to in FlyBase by the symbol Hsc70-1 (CG8937, FBgn0001216). It has the cytological map location 70C9. Its sequence location is 3L:13967277..13970879. Its molecular function is described as: ATPase activity; unfolded protein binding; ATP binding. It is involved in the biological processes: protein folding; response to heat. One allele is reported. No phenotypic data is available. It has 3 annotated transcripts and 3 annotated polypeptides.

Detailed Mapping Data

FlyBase Computed Cytological Location

Cytogenetic map

Evidence for location

70C9-70C9

Limits computationally determined from genome sequence between P{EP}EP3561^EP3561 and P{PZ}l(3)70Da⁰²⁴⁰²&P{PZ}btl⁰⁰²⁰⁸

Experimentally Determined Cytological Location

Cytogenetic map

Notes

References

Experimentally Determined Recombination Data

Location

Left of (cM)

Right of (cM)

Notes

Molecular Map Data

Gene Order (in direction of increasing cytology)

References

Gene Order (overall orientation not stated)

References

Gene Model & Products

Please see the GBrowse view of Dmel\Hsc70-1 for information on other features

Comments on Gene Model

Transcript Data

Annotated Transcripts

Name

FlyBase ID

RefSeq ID

Length (nt)

Associated CDS (aa)

Hsc70-1-RA

FBtr0075762

NM_079339

2206

641

Hsc70-1-RC

FBtr0075761

NM_168568

2513

555

Hsc70-1-RD

FBtr0075763

NM_168569

1911

555

Additional Transcript Data & Comments

Reported size (kB)

Comments

External Data

Crossreferences

Polypeptide Data

Annotated Polypeptides

Name

FlyBase ID

Predicted MW (kD)

Length (aa)

Theoretical pI

RefSeq ID

GenBank protein

Hsc70-1-PA

FBpp0075504

70.7

641

5.14

NP_524063

AAF49782

Hsc70-1-PC

FBpp0075503

61.3

555

5.10

NP_729940

AAN11819

Hsc70-1-PD

FBpp0075505

61.3

555

5.10

NP_729941

AAN11820

Additional Polypeptide Data & Comments

Reported size (kD)

Comments

External Data

Linkouts

PANTHER - Protein classification by function, families, and pathways

•

FBgn0001216

Crossreferences

InterPro domains - A database of protein families, domains, and functional sites

•

Heat shock protein Hsp70 (IPR001023)

Heat shock protein 70 (IPR013126)

MitoDrome - A database of annotated Dmel nuclear genes encoding mitochondrial proteins

•

MTDROME08937

Sequences Consistent with the Gene Model

DDBJ /
EMBL /
GenBank

DNA sequence

Protein sequence

Name

P29843

UniProtKB/TrEMBL

Maps to

Does NOT map to

Identified with

(BDGP Project Members, 2000-)

(Oliver, 2001.8.16)

Mapped Features & Mutations

Please see GBrowse or insertion reports for information on insertions of transgenic constructs and features not listed here

Type

Symbol & Location

Additional Notes

References

External Data

Linkouts

Crossreferences

Expression Data

FlyBase-Curated Data

Transcript and
Protein data

Please see the FlyBase Gene Expression Report for details of gene expression from the literature.

Summary of Transcript Expression

Stage

Tissue/Position

Reference

Marker for

Subcellular Localization

CV Term

Summary of Polypeptide Expression

Stage

Tissue/Position

Reference

Marker for

Subcellular Localization

CV Term

External Data & Images

Linkouts

FLIGHT - Cell culture data for RNAi and other high-throughput technologies

•

FBgn0001216

GEO (NCBI) - Gene expression data: microarray and other high-throughput technologies

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FBgn0001216

Alleles & Phenotypes

Summary of Allele Phenotypes

Phenotype manifest in

Allele

Classical Alleles ( 0 )

For All Classical Alleles Show

Allele of Hsc70-1	Class	Mutagen	Stocks	Known lesion

Alleles Carried on Transgenic Constructs ( 1 )

For All Alleles Carried on Transgenic Constructs Show

Allele of Hsc70-1	Class	Mutagen	Stocks	Known lesion
Hsc70-1^GD13532		in vitro construct \| RNAi in vitro construct \| regulatory fusion	2	Yes

Aneuploid Aberrations

Useful deficiency

Df(3L)fz-GF3b

Useful duplication

Dp(3;3)M71

Transgenic Constructs & Insertions

Transgenic Constructs

Type of construct

Name

Expression data

UAS construct

P{GD13532}

Insertions

Type of insertions

Name

Expression data

Related Comments

Please look at the allele reports for the complete phenotype data

First recognised by sequence similarity to Hsp70. Transcription is developmentally regulated, but is temperature independent.

Members of the hsc70 gene family (heat shock cognate genes) that reside within the same intracellular compartment in different organisms share greater amino acid identity than hsc70 proteins from the same organism but different organelles. This pattern of conservation indicates specialisation of hsc70 function.

(Rubin et al., 1993)

Gene Ontology: Function, Process & Cellular Component ( 7 )

Molecular Function

CV term

Evidence

References

ATP binding

inferred from electronic annotation with InterPro:IPR001023

(FlyBase Curators et al., 2004-)

ATPase activity

non-traceable author statement

(Rubin, 1992)

unfolded protein binding

inferred from sequence or structural similarity with MGD:Hspa8; MGI:MGI:105384

(FlyBase, 1992-)

Biological Process

CV term

Evidence

References

protein folding

inferred from sequence or structural similarity with MGD:Hspa8; MGI:MGI:105384

(FlyBase, 1992-)

inferred from sequence or structural similarity with UniProtKB:P38646

(Sardiello et al., 2003.6.11)

response to heat

non-traceable author statement

(Swiss-Prot Project Members, 1993.4.1)

Cellular Component

CV term

Evidence

References

lipid particle

inferred from direct assay

(Beller et al., 2006)

mitochondrion

inferred from sequence or structural similarity with UniProtKB:P38646

(Sardiello et al., 2003.6.11)

Sequence Ontology: Class of Gene

protein_coding_gene

nuclear_gene

Interactions & Pathways

Summary of Genetic Interactions

Interacts with

Please look at the allele data for full details of the genetic interactions

Hsc70-1 allele

Gene

References

External Data

Linkouts

BioGRID - Interaction data, including yeast 2-hybrid and genetic interactions

•

64882

Orthologs

Genome-wide drosophilid orthologs

Dana\GF24052

Dere\GG15660

Dgri\GH14643

Dmoj\GI11627

Dper\GL15719

Dpse\GA23772

Dsec\GM25439

Dsim\GD14466

Dvir\GJ11309

Dwil\GK20316

Dyak\GE21989

Curated drosophilid orthologs

Dbuz\Hsc70-1

(Laayouni et al., 2000)

Dpse\GA21421

Drep\Hsc70-1

Dsim\Hsc70-1

Linkouts

Functional Complementation between Species

Inter-Species Misexpression Data

Produces phenotype in

Produces NO phenotype in

Stocks & Reagents

Stocks Listed in FlyBase ( 2 )

VDRC

v28797

w¹¹¹⁸ P{GD13532}v28797

v45596

w¹¹¹⁸; P{GD13532}v45596

Genomic Clones ( 2 )

cDNA Clones ( 43 )

Please Note

This section lists cDNAs and ESTs that fall within the genomic extent of the gene model, which may include cDNAs and ESTs of genes within introns, or of overlapping genes. Please see GBrowse for alignment of the cDNAs and ESTs to the gene model.

cDNA Clones, Fully Sequenced

BDGP DGC clones

Other clones

cDNA Clones, End Sequenced (ESTs)

BDGP DGC clones

AT29089
AT16637
AT26903
AT09467
AT10102
AT03360
AT19920
AT24079
AT24516
AT03332
AT06214
AT25913
AT08678
AT27702
GM28820
AT16155
AT02718
AT26879
AT26909
AT26980
AT28561
AT25966
AT06287
AT31528
AT04295
AT21737
AT15228
GH14519
AT08838
AT10125
AT10984
AT12112
AT31752

Other clones

RNAi & Array Information

Affy Oligo

Linkouts

DRSC - RNAi screening (Harvard): high-throughput cell culture data and design

•

FBgn0001216

GenomeRNAi - RNAi phenotypes (Heidelberg): high-throughput cell culture data

•

FBgn0001216

Antibody Information

Other Information

Discoverer

Etymology

Identification

Position Effect Variegation Data

Relationship to Other Genes

Source for database identity of

Source for identity of: Hsc70-1 CG8937

(Bayraktaroglu, 1999.11)

Source for database merge of

Source for merge of: Hsc70-1 BcDNA:RH49358 Source for merge of Hsc70-1 BcDNA:RH49358 was a shared cDNA (date:030728).

(FlyBase, 1996-)

Additional comments

Comments About Role

Comments About Molecular Function

Other Comments

transcript abundance embryo: \+ transcript abundance larva: \- transcript abundance adult: ++ the codon specifying amino acid 66 is interrupted by an 1.7 kb insertion

External Crossreferences & Linkouts

Sequence Crossreferences

RefSeq (Transcripts)

•

RefSeq (Proteins)

•

DDBJ / EMBL / GenBank

•