Gene Dmel\g

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General Information

Symbol

Dmel\g

Species

D. melanogaster

Name

garnet

Annotation symbol

CG10986

Feature type

protein_coding_gene

FlyBase ID

FBgn0001087

Created / Updated

2003-12-01/2003-12-01

Genomic Location

Chromosome (arm)

Recombination map

1-44.4

Cytogenetic map

12B4-12B4

Sequence location

X:13,621,236..13,630,311 [-]

Map ( GBrowse )

Summary Information

Automatically generated summary

See sections below for more information

The gene garnet is referred to in FlyBase by the symbol g (CG10986, FBgn0001087). It has the cytological map location 12B4. Its sequence location is X:13621236..13630311. Its molecular function is described as: protein transporter activity; protein binding. It is involved in the biological processes described with 12 unique terms, many of which group under: transport; eye pigmentation; ocellus pigmentation; cellular localization; organelle organization and biogenesis; cellular biosynthetic process; ocellus pigment biosynthetic process; compound eye pigmentation; determination of adult life span; post-Golgi vesicle-mediated transport; cellular pigmentation; vacuolar transport. 70 alleles are reported. The phenotypes of these alleles are annotated with: pigment cell; Malpighian tubule; testis sheath; pigment granule; eye. It has one annotated transcript and one annotated polypeptide.

Phenotypic Description from the Red Book (Lindsley & Zimm 1992)

Gene/Allele symbols may differ from current usage

g: garnet

Eye color brownish, darkening with age; both pteridine and ommochrome pigments variably reduced in different alleles, giving rise to such descriptions as deep purplish ruby, pinkish, brownish, yellowish ruby, orange, etc. Nolte (1959, Heredity 13: 233-41) measured both pigments spectroscopically for g alleles (g1,g2,g3,g4) and detected subtle differences. Hexter (1963, Proc. Nat. Acad. Sci. USA 50: 372-79) claims g1,g2,g3, and g4 indistinguishable phenotypically but g53d to be orange eyed and easily separable. Earlier authors claims g2 lighter than g1. Pigmentation of Malpighian tubules also reduced (Brehme and Demerec, 1942, Growth 6: 351-56). Eye color (g2) autonomous in transplants into wild type host (Beadle and Ephrussi, 1936, Genetics 21: 225-47).

Detailed Mapping Data

FlyBase Computed Cytological Location

Cytogenetic map

Evidence for location

12B4-12B4

Limits computationally determined from genome sequence between P{EP}NFAT^EP1390&P{EP}CG11164^EP1396 and P{EP}g^EP514

Experimentally Determined Cytological Location

Cytogenetic map

Notes

References

12B-12B

Determined by comparing Celera genomic sequence with sequence from BDGP BAC and P1 clones.

(Lloyd et al., 2000)

12B6-12B7

(Lloyd, 1995.7.10)

Experimentally Determined Recombination Data

Location

1-44.4

(Williams, 1956)

Left of (cM)

nonA

(Kulkarni et al., 1988)

Right of (cM)

(Deak et al., 1982)

mus106

(Smith et al., 1980)

Notes

Molecular Map Data

Gene Order (in direction of increasing cytology)

References

Gene Order (overall orientation not stated)

References

Overall orientation not stated: g- l(1)dd4- CG11134+ CG11151+ l(1)dd4-

(Barbosa et al., 2000)

Gene Model & Products

Please see the GBrowse view of Dmel\g for information on other features

Comments on Gene Model

GC splice donor site postulated

Transcript Data

Annotated Transcripts

Name

FlyBase ID

RefSeq ID

Length (nt)

Associated CDS (aa)

g-RB

FBtr0073842

NM_080046

4172

1034

Additional Transcript Data & Comments

Reported size (kB)

4.0, 3.8 (northern blot)

(Ooi et al., 1997)

Comments

External Data

Crossreferences

Polypeptide Data

Annotated Polypeptides

Name

FlyBase ID

Predicted MW (kD)

Length (aa)

Theoretical pI

RefSeq ID

GenBank protein

g-PB

FBpp0073673

114.8

1034

7.31

NP_524785

AAF48307

Additional Polypeptide Data & Comments

Reported size (kD)

1034 (aa); 115 (kD predicted)

(Ooi et al., 1997)

Comments

External Data

Linkouts

PANTHER - Protein classification by function, families, and pathways

•

FBgn0001087

Crossreferences

InterPro domains - A database of protein families, domains, and functional sites

•

Clathrin/coatomer adaptor, adaptin-like, N-terminal (IPR002553)

Armadillo-like helical (IPR011989)

Armadillo-type fold (IPR016024)

Adaptor protein complex AP-3, delta subunit (IPR017105)

Sequences Consistent with the Gene Model

DDBJ /
EMBL /
GenBank

DNA sequence

Protein sequence

Name

P54362

UniProtKB/TrEMBL

Maps to

Does NOT map to

Identified with

RE06749

(BDGP Project Members, 2000-)

Mapped Features & Mutations

Please see GBrowse or insertion reports for information on insertions of transgenic constructs and features not listed here

Type

Symbol & Location

Additional Notes

References

External Data

Linkouts

DEDB - Drosophila exon database: splicing graphs

•

2747

Crossreferences

Expression Data

FlyBase-Curated Data

Transcript and
Protein data

Please see the FlyBase Gene Expression Report for details of gene expression from the literature.

Summary of Transcript Expression

Stage

Tissue/Position

Reference

adult stage

(Ooi et al., 1997)

Marker for

Subcellular Localization

CV Term

Summary of Polypeptide Expression

Stage

Tissue/Position

Reference

Marker for

Subcellular Localization

CV Term

External Data & Images

Linkouts

FLIGHT - Cell culture data for RNAi and other high-throughput technologies

•

FBgn0001087

FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array

•

FBgn0001087

GEO (NCBI) - Gene expression data: microarray and other high-throughput technologies

•

FBgn0001087

Alleles & Phenotypes

Summary of Allele Phenotypes

Lethality

Allele

viable | male

g^49h

viable | female | reduced

g^49h

viable | reduced

g^tuh2

lethal | male

lethal | embryonic stage | recessive

g^Mc19-1

Other Phenotypes

Allele

wild-type

visible

eye color defective

eye color defective (with Df(1)HA92)

eye color defective | cell autonomous

eye color defective | recessive

eye color defective | male

g⁶⁰

body color defective | recessive

short lived | male

behavior defective | recessive

courtship defective | male

Sterility

Allele

male sterile

g^17B

fertile | reduced

g^tuh2

female fertile | reduced

Phenotype manifest in

Allele

g^53d

pigment cell (with Df(1)HA92)

Classical Alleles ( 64 )

For All Classical Alleles Show

Allele of g	Class	Mutagen	Stocks	Known lesion
g^17B		X ray	0	--
g¹	hypomorph	spontaneous	14	Yes
g^2.15.43b		spontaneous	0	--
g^26-10		X ray	0	--
g^26-15		sulfur mustard	0	--
g^26-41		sulfur mustard	0	--
g^271-10		X ray	0	--
g^271-2		X ray	0	--
g^271-6		X ray	0	--
g^271-9		X ray	0	--
g^28-10		sulfur mustard	0	--
g^28-40		sulfur mustard	0	--
g^29h		spontaneous	0	--
g²	hypomorph	spontaneous	36	Yes
g^2rv			1	--
g^30d		spontaneous	0	--
g^32d		spontaneous	0	--
g^33j		spontaneous	0	--
g^33l		spontaneous	0	--
g^34e		spontaneous	0	--
g^37c		spontaneous	0	--
g^37f		spontaneous	0	--
g^37k		spontaneous	0	--
g^38b		spontaneous	0	--
g³	hypomorph	spontaneous	0	Yes
g^42a		X ray	0	--
g^49h		<up>32</up>P	0	--
g⁴		X ray	34	Yes
g^50e			1	Yes
g^53d		spontaneous	1	Yes
g^55k		spontaneous	0	--
g⁶⁰		spontaneous	0	--
g⁶¹			0	Yes
g^64b		X ray	0	--
g^68d		ethyl methanesulfonate	0	--
g^70k		spontaneous	0	--
g^76b			0	--
g⁷⁹ⁱ		spontaneous	0	--
g^81d			0	--
g^B166		X ray	1	Yes
g^EMS			0	Yes
g^EP514		P-element activity	2	--
g^F		spontaneous	0	--
g^Mc19-1		ethyl methanesulfonate	0	--
g^P-Rev1		P-element activity	0	Yes
g^P-Rev2		P-element activity	0	Yes
g^P-Rev3		P-element activity	0	Yes
g^P-Rev4		P-element activity	0	Yes
g^P2		<up>32</up>P	0	--
g^P	hypomorph	P-element activity	0	Yes
g^R1			0	--
g^S1		spontaneous	0	--
g^S2		spontaneous	0	Yes
g^S3		spontaneous	0	Yes
g^X		X ray	1	Yes
g^Y1		spontaneous	0	--
g^e		cold-treatment	0	--
g^f00207		piggyBac transposase	1	--
g^im			0	Yes
g^l			0	--
g^tuh1		spontaneous	0	--
g^tuh2		spontaneous	0	--
g^unspecified			0	--
g^w		X ray	1	--

Alleles Carried on Transgenic Constructs ( 6 )

For All Alleles Carried on Transgenic Constructs Show

Allele of g	Mutagen	Stocks	Known lesion
g^GD6405	in vitro construct \| RNAi in vitro construct \| regulatory fusion	2	Yes
g^GD7158	in vitro construct \| RNAi in vitro construct \| regulatory fusion	3	Yes
g^t9	in vitro construct \| genomic fragment	0	Yes
g^tB10	in vitro construct \| genomic fragment	0	Yes
g^tBa	in vitro construct \| genomic fragment	0	Yes
g^tX9	in vitro construct \| genomic fragment	0	Yes

Aneuploid Aberrations

Useful deficiency

Df(1)HA92

Disrupted in

Df(1)A124

(Mackensen, 1935)

Df(1)AR10

(Muralidhar and Thomas, 1993)

Df(1)AR2

(Muralidhar and Thomas, 1993)

Df(1)BSC546

(Christensen et al., 2008.6.11)

Df(1)Exel6247

(Ryder, 2004.4.26)

Df(1)HA92

(Lloyd et al., 2002, Craymer and Roy, 1980, Deak et al., 1982, Hardy et al., 1984)

Df(1)O4

(Oliver, 1937)

Df(1)g

(Mason et al., 1981, Deak et al., 1982)

Df(1)w68a

(Sorsa et al., 1973)

Df(1)w70e

(Sorsa et al., 1973)

Df(1)w70j

(Sorsa et al., 1973)

Df(1)w71b

(Sorsa et al., 1973)

In(1)g¹⁷

(Valencia, 1966)

Duplicated in

Dp(1;2)A124

Dp(1;3)ras^v

Dp(1;f)LJ9

(Hardy et al., 1984, Livingstone, 1985)

Not duplicated in

Dp(1;1)100

(Dobzhansky, 1932)

Dp(1;1)105

(Dobzhansky, 1932)

Dp(1;1)112

(Dobzhansky, 1932)

Dp(1;3)v⁺74c

(Schalet, 1986)

Dp(1;3)w^68a

Dp(1;4)r⁺l

(Schalet, 1986)

Dp(1;f)102

(Dobzhansky, 1932)

Dp(1;f)106

(Dobzhansky, 1932)

Dp(1;f)107

(Dobzhansky, 1932)

Dp(1;f)118

(Dobzhansky, 1932)

Dp(1;f)136

(Dobzhansky, 1932)

Dp(1;f)137

(Dobzhansky, 1932)

Dp(1;f)LJ4

(Livingstone, 1985)

Dp(1;f)y⁺

(Zusman et al., 1994)

Not disrupted in

Df(1)172

(Patterson, 1932)

Df(1)231c

(Patterson, 1932)

Df(1)235

(Patterson, 1932)

Df(1)247a

(Patterson, 1932)

Df(1)247g

(Patterson, 1932)

Df(1)262

(Patterson, 1932)

Df(1)267

(Patterson, 1932)

Df(1)271

(Patterson, 1932)

Df(1)274

(Patterson, 1932)

Df(1)303

(Patterson, 1932)

Df(1)308

(Patterson, 1932)

Df(1)314

(Patterson, 1932)

Df(1)354

(Patterson, 1932)

Df(1)p116D

(Yamamoto et al., 2000)

Df(1)p205A

(Yamamoto et al., 2000)

Df(1)p281A

(Yamamoto et al., 2000)

Df(1)p490D

(Yamamoto et al., 2000)

Df(1)w70k

(Sorsa et al., 1973)

In(1)w^-70L26.3

(Sorsa et al., 1973)

T(1;4)231b

(Patterson, 1932)

Transgenic Constructs & Insertions

Transgenic Constructs

Type of construct

Name

Expression data

UAS construct

P{GD6405}

P{GD7158}

Insertions

Type of insertions

Name

Expression data

miscellaneous insertions

?{}g¹

?{}g³

?{}g⁴

?{}g⁶¹

insertion of mobile activating element

P{EP}g^EP514

Related Comments

Please look at the allele reports for the complete phenotype data

Pigmentation of Malpighian tubules also reduced.

(Brehme and Demerec, 1942)

Lesions in g reduce or eliminate pigmentation in the eyes and ocelli and block pigmentation of the fat body and tubules: g is required for normal pigmentation of all four tissues.

(Tearle, 1991)

(Lindsley and Zimm, 1992)

Gene Ontology: Function, Process & Cellular Component ( 19 )

Molecular Function

CV term

Evidence

References

protein binding

inferred from electronic annotation with InterPro:IPR002553, InterPro:IPR017105

(FlyBase Curators et al., 2004-)

protein transporter activity

inferred from electronic annotation with InterPro:IPR017105