Gene Dmel\dup

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General Information

Symbol

Dmel\dup

Species

D. melanogaster

Name

double parked

Annotation symbol

CG8171

Feature type

protein_coding_gene

FlyBase ID

FBgn0000996

Created / Updated

2003-12-02/2003-12-02

Genomic Location

Chromosome (arm)

Recombination map

Cytogenetic map

51F11-51F11

Sequence location

2R:11,264,119..11,268,465 [-]

Map ( GBrowse )

Summary Information

Automatically generated summary

See sections below for more information

The gene double parked is referred to in FlyBase by the symbol dup (CG8171, FBgn0000996). It has the cytological map location 51F11. Its sequence location is 2R:11264119..11268465. Its molecular function is described as DNA binding. It is involved in the biological processes: chorion-containing eggshell formation; DNA replication; DNA replication checkpoint; DNA-dependent DNA replication; eggshell chorion gene amplification; antimicrobial humoral response. 18 alleles are reported. The phenotypes of these alleles are annotated with: mitotic cell cycle; imaginal disc; mitotic metaphase; cycle 16 embryo; S phase; kinetochore; follicle cell; eye disc. It has one annotated transcript and one annotated polypeptide.

External Summaries

Detailed Mapping Data

FlyBase Computed Cytological Location

Cytogenetic map

Evidence for location

51F11-51F11

Limits computationally determined from genome sequence between P{PZ}scb⁰¹²⁸⁸ and P{lacW}dup^k03308

Experimentally Determined Cytological Location

Cytogenetic map

Notes

References

51F11-51F12

(determined by in situ hybridisation)

(Spradling et al., 1999)

51F11-51F12

(BDGP Project Members, 1994-1999)

Experimentally Determined Recombination Data

Location

Left of (cM)

Right of (cM)

Notes

Molecular Map Data

Gene Order (in direction of increasing cytology)

References

Gene Order (overall orientation not stated)

References

Gene Model & Products

Please see the GBrowse view of Dmel\dup for information on other features

Comments on Gene Model

Transcript Data

Annotated Transcripts

Name

FlyBase ID

RefSeq ID

Length (nt)

Associated CDS (aa)

dup-RA

FBtr0087387

NM_080139

3115

743

Additional Transcript Data & Comments

Reported size (kB)

Comments

External Data

Crossreferences

Polypeptide Data

Annotated Polypeptides

Name

FlyBase ID

Predicted MW (kD)

Length (aa)

Theoretical pI

RefSeq ID

GenBank protein

dup-PA

FBpp0086519

83.5

743

10.46

NP_524878

AAF58141

Additional Polypeptide Data & Comments

Reported size (kD)

Comments

External Data

Linkouts

Crossreferences

InterPro domains - A database of protein families, domains, and functional sites

•

DNA replication factor CDT1-like (IPR014939)

Sequences Consistent with the Gene Model

DDBJ /
EMBL /
GenBank

DNA sequence

Protein sequence

Name

Q7JVY2

Maps to

Does NOT map to

Identified with

(Spradling et al., 1999)

LD12389

(Whittaker, 2000.6.15)

LD35784

(BDGP Project Members, 2000-)

Mapped Features & Mutations

Please see GBrowse or insertion reports for information on insertions of transgenic constructs and features not listed here

Type

Symbol & Location

Additional Notes

References

External Data

Linkouts

DEDB - Drosophila exon database: splicing graphs

•

12417

Crossreferences

Expression Data

FlyBase-Curated Data

Transcript and
Protein data

Please see the FlyBase Gene Expression Report for details of gene expression from the literature.

Summary of Transcript Expression

Stage

Tissue/Position

Reference

Marker for

Subcellular Localization

CV Term

Summary of Polypeptide Expression

Stage

Tissue/Position

Reference

Marker for

Subcellular Localization

CV Term

External Data & Images

Linkouts

FLIGHT - Cell culture data for RNAi and other high-throughput technologies

•

FBgn0000996

FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array

•

FBgn0000996

GEO (NCBI) - Gene expression data: microarray and other high-throughput technologies

•

FBgn0000996

Alleles & Phenotypes

Summary of Allele Phenotypes

Lethality

Allele

lethal

lethal | recessive

dup^k03308

lethal | embryonic stage | recessive

Other Phenotypes

Allele

cell death increase

mitotic cell cycle defective

mitotic cell cycle defective (with dup^PA77)

dup^a1

mitotic cell cycle defective (with dup^a1)

dup^PA77

mitotic cell cycle defective | maternal effect

dup^unspecified

Sterility

Allele

female sterile

dup^PA77

Phenotype manifest in

Allele

follicle cell

imaginal disc

mitotic cell cycle (with dup^a1)

dup^PA77

mitotic cell cycle

mitotic cell cycle (with dup^PA77)

dup^a1

cycle 16 embryo & S phase

dup^a1

cycle 16 embryo

mitosis & nuclear chromosome

mitotic metaphase

S phase

eye disc

dup^{hsp70.T:Hsap\MYC}

kinetochore | maternal effect

dup^unspecified

Classical Alleles ( 12 )

For All Classical Alleles Show

Allele of dup	Mutagen	Stocks	Known lesion
dup^CB04090	P-element activity	0	Yes
dup^EP2060	P-element activity	1	--
dup^PA77	ethyl methanesulfonate	2	Yes
dup^a1		1	Yes
dup^a2		0	--
dup^a3		1	Yes
dup^a4		0	--
dup^c05735	piggyBac transposase	0	--
dup^c05782	piggyBac transposase	0	--
dup^k03308	P-element activity	3	Yes
dup^l5	γ ray	0	--
dup^unspecified		0	--

Alleles Carried on Transgenic Constructs ( 6 )

For All Alleles Carried on Transgenic Constructs Show

Allele of dup	Mutagen	Stocks	Known lesion
dup^{10(A).hsp70.T:Hsap\MYC}	in vitro construct \| site directed mutagenesis	0	Yes
dup^{C.hsp70.T:Hsap\MYC}	in vitro construct \| regulatory fusion in vitro construct \| coding region fusion	0	Yes
dup^GD13203	in vitro construct \| RNAi in vitro construct \| regulatory fusion	2	Yes
dup^{N.hsp70.T:Hsap\MYC}	in vitro construct \| site directed mutagenesis	0	Yes
dup^dsRNA.cRa	in vitro construct \| RNAi	0	Yes
dup^{hsp70.T:Hsap\MYC}	in vitro construct \| regulatory fusion in vitro construct \| coding region fusion	0	Yes

Aneuploid Aberrations

Useful deficiency

Df(2R)l4

Useful duplication

Dp(2;2)SMG45

Disrupted in

Df(2R)BSC346

(Christensen and Cook, 2007.10.29)

Df(2R)Exel9015

(Ryder, 2004.4.26)

Df(2R)Jp1

(Spradling et al., 1999, Sekelsky et al., 2000, Whittaker et al., 2000, BDGP Project Members, 1994-1999)

Df(2R)XTE-11

(Underwood et al., 1990)

Df(2R)XTE-18

(Sekelsky et al., 2000, Underwood et al., 1990, Schupbach and Wieschaus, 1991)

Df(2R)XTED4

(Underwood et al., 1990)

Df(2R)l13

(Underwood et al., 1990, Smith et al., 1993)

Df(2R)l4

(Underwood et al., 1990)

Not disrupted in

Df(2R)Jp4

(Spradling et al., 1999, Sekelsky et al., 2000, BDGP Project Members, 1994-1999)

Df(2R)Jp5

(BDGP Project Members, 1994-1999)

Df(2R)XTE-58

(Underwood et al., 1990)

Df(2R)XTED1

(Underwood et al., 1990)

Df(2R)knSA3

(Sekelsky et al., 2000)

Df(2R)l4

(Underwood et al., 1990)

Transgenic Constructs & Insertions

Transgenic Constructs

Type of construct

Name

Expression data

UAS construct

P{GD13203}

heat-shock construct

P{hsp70-dup.10(A).MYC}

P{hsp70-dup.C.MYC}

P{hsp70-dup.MYC}

P{hsp70-dup.N.MYC}

Insertions

Type of insertions

Name

Expression data

miscellaneous insertions

PBac{PB}dup^c05735

PBac{PB}dup^c05782

P{PTT-GB}dup^CB04090

insertion of enhancer trap

P{lacW}dup^k03308

P{lacW}l(2)SH0686^SH0686

insertion of mobile activating element

P{EP}dup^EP2060

P{Mae-UAS.6.11}B0323^B0323

Related Comments

Please look at the allele reports for the complete phenotype data

Mutations at the fs(2)51Fa locus cause defects in midoogenesis.

(Schupbach and Wieschaus, 1991)

dup gene product is essential for proper DNA replication.

(Smith et al., 1993)

Homozygous embryos show dramatically reduced levels of DNA replication after the 15th S phase.

(Whittaker et al., 1999)

RNAi generated by PCR using primers directed to this gene causes a cell growth and viability phenotype when assayed in Kc167 and S2R⁺ cells.

(Boutros et al., 2004)

RNAi screen using dsRNA made from templates generated with primers directed against this gene results in the formation of compact chromatin when assayed in S2 cells. This phenotype can be observed when the screen is performed with or without Cdc27 dsRNA.

(Goshima et al., 2007)

Gene Ontology: Function, Process & Cellular Component ( 11 )

Molecular Function

CV term

Evidence

References

DNA binding

non-traceable author statement

(Whittaker, 2000.6.15)

Biological Process

CV term

Evidence