Gene Dmel\fs(1)Yb

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General Information

Symbol

Dmel\fs(1)Yb

Species

D. melanogaster

Name

female sterile (1) Yb

Annotation symbol

CG2706

Feature type

protein_coding_gene

FlyBase ID

FBgn0000928

Created / Updated

2003-12-01/2003-12-01

Genomic Location

Chromosome (arm)

Recombination map

1-1.8

Cytogenetic map

3B3-3B3

Sequence location

X:2,614,501..2,618,192 [+]

Map ( GBrowse )

Summary Information

Automatically generated summary

See sections below for more information

The gene female sterile (1) Yb is referred to in FlyBase by the symbol fs(1)Yb (CG2706, FBgn0000928). It has the cytological map location 3B3. Its sequence location is X:2614501..2618192. Its molecular function is unknown. It is involved in the biological processes: germ-line stem cell division; germ-line stem cell maintenance; germ cell development; somatic stem cell division; ovarian follicle cell stalk formation; oogenesis. 19 alleles are reported. The phenotypes of these alleles are annotated with 14 unique terms, many of which group under: organ system; anatomical structure; organism; germarium; egg; follicle cell; female reproductive system; germ cell; spectrosome; cytoplasmic part. It has one annotated transcript and one annotated polypeptide.

Phenotypic Description from the Red Book (Lindsley & Zimm 1992)

Gene/Allele symbols may differ from current usage

fs(1)Yb

Homozygous females semisterile; lay reduced numbers of eggs, most of which remain vacuolated after deposition; a few develop into normal-appearing adults. Mutation without effect on males.

Detailed Mapping Data

FlyBase Computed Cytological Location

Cytogenetic map

Evidence for location

3B3-3B3

Limits computationally determined from genome sequence between P{EP}EP1362 and P{EP}dnc^EP1395

Experimentally Determined Cytological Location

Cytogenetic map

Notes

References

3B1-3B1

Determined by deficiency mapping (details unspecified).

(Swan et al., 2001)

3C1-3C1

(King, 1999.4.8)

Experimentally Determined Recombination Data

Location

1-1.8

Left of (cM)

Right of (cM)

l(1)3Bd

Notes

Molecular Map Data

Gene Order (in direction of increasing cytology)

References

In direction of increasing cytology: CG2701- fs(1)Yb+ fs(1)Ya+

(King and Lin, 1999)

Gene Order (overall orientation not stated)

References

Overall orientation not stated: crm? dwg? fs(1)Yb?

(Gaszner et al., 1999)

Gene Model & Products

Please see the GBrowse view of Dmel\fs(1)Yb for information on other features

Comments on Gene Model

DGC clone appears problematic (AT22217): incomplete CDS.

Transcript Data

Annotated Transcripts

Name

FlyBase ID

RefSeq ID

Length (nt)

Associated CDS (aa)

fs(1)Yb-RA

FBtr0070484

NM_058146

3503

1042

Additional Transcript Data & Comments

Reported size (kB)

4.1 (northern blot)

(King and Lin, 1999)

Comments

External Data

Crossreferences

Polypeptide Data

Annotated Polypeptides

Name

FlyBase ID

Predicted MW (kD)

Length (aa)

Theoretical pI

RefSeq ID

GenBank protein

fs(1)Yb-PA

FBpp0070462

117.5

1042

7.02

NP_477494

AAF45815

Additional Polypeptide Data & Comments

Reported size (kD)

1042 (aa); 117 (kD predicted)

(King and Lin, 1999)

Comments

External Data

Linkouts

PANTHER - Protein classification by function, families, and pathways

•

FBgn0000928

Crossreferences

Sequences Consistent with the Gene Model

DDBJ /
EMBL /
GenBank

DNA sequence

Protein sequence

Name

Maps to

95B7

(FlyBase, 1992-)

Does NOT map to

Identified with

AT22217

(BDGP Project Members, 2000-)

Mapped Features & Mutations

Please see GBrowse or insertion reports for information on insertions of transgenic constructs and features not listed here

Type

Symbol & Location

Additional Notes

References

point mutation

fs(1)Yb[72]
X:2,615,063..2,615,063

comment=TGG to TAG
evidence=experimental
pr_change=W135@|fs(1)Yb-PA
reported_na_change=G566A
reported_pr_change=?134@

(King et al., 2001)

point mutation

fs(1)Yb[M104-1]
X:2,616,736..2,616,736

evidence=experimental
na_change=G2616736A
pr_change=D693N|fs(1)Yb-PA
reported_na_change=G2239A
reported_pr_change=D?N

(King and Lin, 1999)

point mutation

fs(1)Yb[M104-3]
X:2,617,070..2,617,070

evidence=experimental
na_change=G2617070A
pr_change=C804Y|fs(1)Yb-PA
reported_na_change=G2573A
reported_pr_change=C?Y

(King and Lin, 1999)

point mutation

fs(1)Yb[1]
X:2,617,414..2,617,414

evidence=experimental
na_change=G2617414A
pr_change=R898Q|fs(1)Yb-PA
reported_na_change=G2916A
reported_pr_change=R?Q

(King and Lin, 1999)

rescue fragment

fs(1)Yb[+t8.5]
X:2,613,320..2,621,440

comment=SalI-SalI fragment contains both the fs(1)Yb and fs(1)Ya transcription units and part of the CG2701 transcription unit, and rescues both fs(1)Ya[2] and fs(1)Yb[1]. fs(1)Yb[+t8.5] is the same construct as fs(1)Ya[+t8.5], fs(1)Ya[+t8.1], fs(1)Yb[t8.1] and CG2701[t8.1]. Position of restriction fragment on reference sequence inferred by FlyBase curator.
evidence=experimental
linked_to=SalI-SalI_rfrag

(Lin and Wolfner, 1989, Lin and Wolfner, 1991, King and Lin, 1999)

External Data

Linkouts

DEDB - Drosophila exon database: splicing graphs

•

Crossreferences

Expression Data

FlyBase-Curated Data

Transcript and
Protein data

Please see the FlyBase Gene Expression Report for details of gene expression from the literature.

Summary of Transcript Expression

Stage

Tissue/Position

Reference

oogenesis stage,adult stage

terminal filament

(King and Lin, 1999)

larval stage,pupal stage,adult stage

(King and Lin, 1999)

oogenesis stage,adult stage

ovariole

(King and Lin, 1999)

oogenesis stage,adult stage

epithelial sheath

(King and Lin, 1999)

Marker for

Subcellular Localization

CV Term

Summary of Polypeptide Expression

Stage

Tissue/Position

Reference

Marker for

Subcellular Localization

CV Term

External Data & Images

Linkouts

FLIGHT - Cell culture data for RNAi and other high-throughput technologies

•

FBgn0000928

FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array

•

FBgn0000928

GEO (NCBI) - Gene expression data: microarray and other high-throughput technologies

•

FBgn0000928

Alleles & Phenotypes

Summary of Allele Phenotypes

Lethality

Allele

viable

fs(1)Yb⁷²

lethal | embryonic stage | maternal effect | recessive

fs(1)Yb¹

Other Phenotypes

Allele

wild-type

Sterility

Allele

female sterile

fs(1)Yb⁷²

female sterile | recessive

female semi-sterile | recessive

fs(1)Yb¹

male fertile

fs(1)Yb⁷²

female fertile

fs(1)Yb^t8.1

Phenotype manifest in

Allele

egg | maternal effect

fs(1)Yb¹

fs(1)Yb⁷²

fs(1)Yb^M104-1

nurse cell spectrosome, with Scer\GAL4^hs.PB

fs(1)Yb^Scer\UAS.cKa

female germline stem cell | supernumerary, with Scer\GAL4^hs.PB

fs(1)Yb^Scer\UAS.cKa

interfollicle cell | supernumerary, with Scer\GAL4^hs.PB

fs(1)Yb^Scer\UAS.cKa

Classical Alleles ( 8 )

For All Classical Alleles Show

Allele of fs(1)Yb	Class	Mutagen	Stocks	Known lesion
fs(1)Yb¹		ethyl methanesulfonate	0	Yes
fs(1)Yb²		ethyl methanesulfonate	0	--
fs(1)Yb³		ethyl methanesulfonate	0	--
fs(1)Yb⁷²		ethyl methanesulfonate	1	--
fs(1)Yb^M104-1	loss of function	ethyl methanesulfonate	0	--
fs(1)Yb^M104-2	loss of function	ethyl methanesulfonate	2	--
fs(1)Yb^M104-3	loss of function	ethyl methanesulfonate	2	--
fs(1)Yb^unspecified			0	--

Alleles Carried on Transgenic Constructs ( 11 )

For All Alleles Carried on Transgenic Constructs Show

Allele of fs(1)Yb	Mutagen	Stocks	Known lesion
fs(1)Yb^GD9813	in vitro construct \| RNAi in vitro construct \| regulatory fusion	2	Yes
fs(1)Yb^Scer\UAS.cKa	in vitro construct \| regulatory fusion	0	Yes
fs(1)Yb^dsRNA.cDa	in vitro construct \| RNAi	0	Yes
fs(1)Yb^{hs.T:Zzzz\His6,T:Zzzz\FLAG}	in vitro construct \| regulatory fusion in vitro construct \| coding region fusion	0	Yes
fs(1)Yb^t1.8	in vitro construct \| genomic fragment	0	Yes
fs(1)Yb^t3.9	in vitro construct \| genomic fragment	0	Yes
fs(1)Yb^t4.6	in vitro construct \| genomic fragment	0	Yes
fs(1)Yb^t6.4	in vitro construct \| genomic fragment	0	Yes
fs(1)Yb^t7.2	in vitro construct \| genomic fragment	0	Yes
fs(1)Yb^t7.7	in vitro construct \| genomic fragment	0	Yes
fs(1)Yb^t8.1	in vitro construct \| genomic fragment	0	Yes

Aneuploid Aberrations

Useful deficiency

Df(1)w258-45

Useful duplication

Dp(1;2)w⁺70h

Disrupted in

Df(1)64c4

(Thierry-Mieg, 1982)

Df(1)X12

(Thierry-Mieg, 1982)

Df(1)w-rJ1

(Thierry-Mieg, 1982)

Df(1)w258-11

(Thierry-Mieg, 1982)

Df(1)w258-42

(Thierry-Mieg, 1982)

Df(1)w258-45

(Thierry-Mieg, 1982, Lin and Wolfner, 1989)

Not disrupted in

Df(1)62g18

(Thierry-Mieg, 1982)

Df(1)64f1

(Thierry-Mieg, 1982, Lin and Wolfner, 1989)

Df(1)64j4

(Thierry-Mieg, 1982, Lin and Wolfner, 1989)

Df(1)65j26

(Thierry-Mieg, 1982)

Df(1)K95

(Thierry-Mieg, 1982)

Df(1)N-8

(Lin and Wolfner, 1989)

Df(1)TEM7

(Thierry-Mieg, 1982)

Df(1)w55j

(Lin and Wolfner, 1989)

Transgenic Constructs & Insertions

Transgenic Constructs

Type of construct

Name

Expression data

UAS construct

P{GD9813}

P{UAS-Yb.K}

characterization construct

heat-shock construct

Insertions

Type of insertions

Name

Expression data

Related Comments

Please look at the allele reports for the complete phenotype data

fs(1)Yb is required for oogenesis and embryogenesis. It is tightly linked to fs(1)Ya.

(Lin and Wolfner, 1989)

fs(1)Yb gene product affects the asymmetric division of the female germline stem cell (GSC).

(Lin and Spradling, 1992)

Mitotic germline recombination demonstrates that fs(1)Yb is soma- dependent.

(Johnson et al., 1992)

fs(1)Yb is required in the soma for ovary follicle cell differentiation and to support later stages of egg maturation. Mutations at fs(1)Yb show genetic interactions with the N group of neurogenic genes.

(Johnson et al., 1995)

Gene Ontology: Function, Process & Cellular Component ( 7 )

Molecular Function

CV term

Evidence

References

Biological Process

CV term

Evidence

References

germ cell development

inferred from mutant phenotype

(King et al., 2001)

germ-line stem cell division

inferred from mutant phenotype

(King and Lin, 1999)

traceable author statement

(Lin, 2002)

traceable author statement

(Deng and Lin, 2001)

germ-line stem cell maintenance

inferred from mutant phenotype

(King and Lin, 1999)

oogenesis

inferred from mutant phenotype

(King and Lin, 1999)

ovarian follicle cell stalk formation

traceable author statement

(Dobens and Raftery, 2000)

somatic stem cell division

traceable author statement

(Lin, 2002)

Cellular Component

CV term

Evidence

References

cytoplasm

inferred from sequence or structural similarity

(King and Lin, 1999)

Sequence Ontology: Class of Gene

protein_coding_gene

nuclear_gene

Interactions & Pathways

Summary of Genetic Interactions

Interacts with

Please look at the allele data for full details of the genetic interactions

fs(1)Yb allele

Gene

References

fs(1)Yb⁷²

unspecified

(Johnson et al., 1995)

mam

(Johnson et al., 1995)

External Data

Linkouts

BioGRID - Interaction data, including yeast 2-hybrid and genetic interactions

•

57793

Orthologs

Genome-wide drosophilid orthologs

Dana\GF22451

Dere\GG18624

Dgri\GH12780

Dmoj\GI15873

Dsec\GM19244

Dvir\GJ18700

Dwil\GK24909

Dyak\GE16277

Curated drosophilid orthologs

Linkouts

Functional Complementation between Species

Inter-Species Misexpression Data

Produces phenotype in

Produces NO phenotype in

Stocks & Reagents

Stocks Listed in FlyBase ( 7 )

VDRC

v25435

w¹¹¹⁸; P{GD9813}v25435

v25437

w¹¹¹⁸; P{GD9813}v25437/TM3

Bloomington

7388

y¹ w¹¹¹⁸ fs(1)Yb⁷² P{neoFRT}19A/FM6

4555

C(1;Y)6, y¹ fs(1)Yb^M104-2 cv¹ v¹/FM7c

4617

y¹ fs(1)Yb^M104-3 cv¹ v¹ f¹/FM0

Kyoto

107794

C(1;Y)6, y¹ fs(1)Yb^M104-2 cv¹ v¹/FM7c

107824

y¹ fs(1)Yb^M104-3 cv¹ v¹ f¹/FM0

Genomic Clones ( 1 )

BACR30B01

cDNA Clones ( 1 )

Please Note

This section lists cDNAs and ESTs that fall within the genomic extent of the gene model, which may include cDNAs and ESTs of genes within introns, or of overlapping genes. Please see GBrowse for alignment of the cDNAs and ESTs to the gene model.

cDNA Clones, Fully Sequenced

BDGP DGC clones

Other clones

cDNA Clones, End Sequenced (ESTs)

BDGP DGC clones

Other clones

SD10010

RNAi & Array Information

Affy Oligo

Linkouts

DRSC - RNAi screening (Harvard): high-throughput cell culture data and design

•

FBgn0000928

GenomeRNAi - RNAi phenotypes (Heidelberg): high-throughput cell culture data

•

FBgn0000928

Antibody Information

Other Information

Discoverer

Young.

Etymology

Identification

Position Effect Variegation Data

Relationship to Other Genes

Source for database identity of

Source for identity of: EG:95B7.8 CG2706

(Deng, 1999.11)

Source for database merge of

Source for merge of: fs(1)Yb EG:95B7.8 Source for merge of fs(1)Yb EG:95B7.8 was sequence comparison (date:991228).

(FlyBase, 1996-)

Additional comments

Comments About Role

fs(1)Yb is required for the maintenance of germline stem cells during oogenesis. The function of fs(1)Yb is soma-dependent.

(King and Lin, 1999)

fs(1)Yb is required for both germline stem cell and somatic stem cell divisions in the ovary.

(King et al., 2001)

Comments About Molecular Function

Other Comments

External Crossreferences & Linkouts

Sequence Crossreferences

RefSeq (Transcripts)

•

NM_058146

RefSeq (Proteins)

•

NP_477494

DDBJ / EMBL / GenBank

•

UniProtKB/TrEMBL

•

Other Crossreferences

Linkouts

BioGRID - Interaction data, including yeast 2-hybrid and genetic interactions

•

57793

DEDB - Drosophila exon database: splicing graphs

•

DRSC - RNAi screening (Harvard): high-throughput cell culture data and design

•

FBgn0000928

FLIGHT - Cell culture data for RNAi and other high-throughput technologies

•

FBgn0000928

FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array

•

FBgn0000928

FlyMine - Integrated genomics database for Drosophila, Anopheles, and C.elegans

•

FBgn0000928

GEO (NCBI) - Gene expression data: microarray and other high-throughput technologies

•

FBgn0000928

GenomeRNAi - RNAi phenotypes (Heidelberg): high-throughput cell culture data

•

FBgn0000928

PANTHER - Protein classification by function, families, and pathways

•

FBgn0000928

Synonyms & Secondary IDs ( 10 )

Reported As

Symbol Synonym

CG2706

(Benos, 2000.12.13, Juhola et al., 2000, )

EG:95B7.8

(Benos et al., 2001, Benos, 2000.12.13, )

fs(1)M104

fs(1)Y^b

(Judd, 1998)

fs(1)Yb

(Gonzalez-Reyes, 2003)

nov

(Szakmary et al., 2005, Gilboa and Lehmann, 2004, Deng and Lin, 2001, Lin, 2002, Szakmary and Lin, 2002, King et al., 2001, Nystul and Spradling, 2006)

Name Synonym

female sterile(1)M104

female sterile (1) Yb

no ovaries

Secondary FlyBase IDs

FBgn0000897
FBgn0024974

References ( 48 )

Generate a list of

All references relating to this gene ( 48 )

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Recent research papers (0)

All research papers listed in FlyBase were published before 2006

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Recent reviews ( 1 )

Nystul and Spradling, 2006, Curr. Opin. Genet. Dev. 16(5): 463--468: Breaking out of the mold: diversity within adult stem cells and their niches. [FBrf0192479]

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