HomeGene Dmel\Actn
| General Information | ||||
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| Symbol | Dmel\Actn | Species | D. melanogaster | |
| Name | α actinin | Annotation symbol | CG4376 | |
| Feature type | protein_coding_gene | FlyBase ID | FBgn0000667 | |
| Created / Updated | 2003-12-01/2003-12-01 | |||
| Genomic Location | ||||
| Chromosome (arm) | X | Recombination map | ||
| Cytogenetic map | 2C4-2C7 | Sequence location | X:1,919,127..1,936,344 [-] | |
| Map ( GBrowse ) | 
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Summary Information
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Automatically generated summary
See sections below for more information | The gene α actinin is referred to in FlyBase by the symbol Actn (CG4376, FBgn0000667). It has the cytological map location 2C4-2C7. Its sequence location is X:1919127..1936344. Its molecular function is described as: actin filament binding; calcium ion binding; actin binding. It is involved in the biological processes: actin filament bundle formation; cytoskeletal anchoring; flight behavior; actin cytoskeleton reorganization. 36 alleles are reported. The phenotypes of these alleles are annotated with: indirect flight muscle; sarcomere; somatic muscle; ovariole; muscle cell; myofibril; egg; third instar larva; eye. It has 3 annotated transcripts and 3 annotated polypeptides. | |||
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Phenotypic Description from the Red Book (Lindsley & Zimm 1992)
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| Gene/Allele symbols may differ from current usage | Actn: α Actinin
The structural gene for α Actinin (Fyrberg). Both
lethal and viable alleles recovered; allelism determined by
Homyk and Emerson. Viable alleles unconditionally flightless;
wing position normal, but unable to fly or beat wings; jump
abnormally short distances. Gynandromorph studies of Actn1
indicate a bilateral pair of submissive foci located mid ventrally close to the embryonic midline (Homyk and Emerson).
ERG normal (Homyk and Pye, 1989, J. Neurogenet. 5: 37-48).
Actn4 is a heat sensitive lethal, and when raised at low temperature, causes aberrant wing display of courting males;
Actn1/Actn4 jumps and flies abnormally when raised at 22 but
normally when raised at 29; temperature sensitive period for
this effect in first half of pupal stage (Homyk et al.,1980).
Trans heterozygotes (i.e., Actn3, Actn4, Actn8, and Actn14)
with hdp-a2 also flightless. Lethal alleles die in late larval
or early pupal stages; homozygous maternal germline clones
produce normal ova. Polyphasic lethality of Actn8 attributed
to position effect of the inversion on arm (Perrimon,
Engstrom, and Mahowald, l985, Genetics 111: 23-41).
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Detailed Mapping Data
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| FlyBase Computed Cytological Location | ||||
Cytogenetic map Evidence for location 2C4-2C7
Limits computationally determined from genome sequence between P{EP}EP427 and P{EP}ActnEP1193&P{EP}CG4322EP1631
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| Experimentally Determined Cytological Location | ||||
Cytogenetic map Notes References 2C7-2C7 (determined by in situ hybridisation)
2C1-2C2 (determined by in situ hybridisation)
2C-2C (determined by in situ hybridisation)
2C1-2C2 (determined by in situ hybridisation)
2C-2C (determined by in situ hybridisation)
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| Experimentally Determined Recombination Data | ||||
| Location | ||||
| Left of (cM) | ||||
| Right of (cM) | ||||
| Notes | ||||
| Molecular Map Data | ||||
Gene Order (in direction of increasing cytology)
References Gene Order (overall orientation not stated) References Overall orientation not stated: Actn+ usp+ Actn+ Overall orientation not stated: CG4290+ CG4313- moody+ CG4325+ Actn+ | ||||
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Gene Model & Products
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Please see the
GBrowse view of
Dmel\Actn
for information on other features
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| Comments on Gene Model | ||||
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Transcript Data
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| Annotated Transcripts | ||||
Name FlyBase ID RefSeq ID Length (nt) Associated CDS (aa) | ||||
| Additional Transcript Data & Comments | ||||
| Reported size (kB) | 3.4, 1.9 (northern blot) | |||
| Comments | ||||
| External Data | ||||
| Crossreferences | ||||
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Polypeptide Data
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| Annotated Polypeptides | ||||
Name FlyBase ID
Predicted MW (kD)
Length (aa)
Theoretical pI
RefSeq ID
GenBank protein
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| Additional Polypeptide Data & Comments | ||||
| Reported size (kD) | 895 (aa); 98 (kD) | |||
| Comments | The EF hands of the Drosophila Actn protein are theoretically capable of binding Ca2+ and this portion of the protein is more similar to the nonmuscle isoform of chicken and the alpha-actinin of Dictyostelium than to the vertebrate sarcomeric and smooth muscle isoforms. Drosophila Actn protein is recognized by a monoclonal antibody made against Lethocerus alpha-actinin. partial CDS encoded by alternate exon | |||
| External Data | ||||
| Linkouts | PANTHER
- Protein classification by function, families, and pathways
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| Crossreferences | InterPro
domains - A database of protein families, domains, and functional sites
• Actinin-type, actin-binding, conserved site (IPR001589)
Calponin-like actin-binding (IPR001715)
Spectrin repeat (IPR002017)
Calcium-binding EF-hand (IPR002048)
EF-Hand type (IPR011992)
EF-hand, Ca insensitive (IPR014837)
Calponin-homology (IPR016146)
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Sequences Consistent with the Gene Model
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| DDBJ
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EMBL / GenBank | DNA sequence Protein sequence Name | |||
| UniProtKB/Swiss-Prot | ||||
| UniProtKB/TrEMBL | ||||
| Maps to | ||||
| Does NOT map to | ||||
| Identified with | ||||
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Mapped Features & Mutations
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Please see
GBrowse
or insertion reports for information on insertions of transgenic
constructs and features not listed here
Type Symbol & Location Additional Notes References aberration junction T(1;3)C212.bk2 X:1,922,783..1,923,808 insertion site Actn[9] X:1,923,883..1,923,885 comment=The codon is interrupted by DNA of unknown origin. evidence=experimental point mutation Actn[1] X:1,924,751..1,924,751 comment=Site of nucleic acid difference inferred by FlyBase based on reported amino acid change evidence=experimental na_change=G1924751A pr_change=G86S|Actn-PC,G86S|Actn-PB,G86S|Actn-PA point mutation Actn[2] X:1,923,908..1,923,908 comment=Site of nucleic acid difference inferred by FlyBase based on reported amino acid change evidence=experimental na_change=C1923908T pr_change=S246F|Actn-PC,S246F|Actn-PB point mutation Actn[3] X:1,923,874..1,923,874 comment=Actn[3] is a silent substitution in a Gln codon from GAG to GAA which is reported to change the context of the adjacent splice donor sequence. evidence=experimental na_change=G1923874A pr_change=Q257Q|Actn-PC,Q257Q|Actn-PB point mutation Actn[4] X:1,919,734..1,919,734 comment=An AG to TG mutation in a splice acceptor site. evidence=experimental na_change=A1919734T | ||||
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External Data
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| Linkouts | DEDB
- Drosophila exon database: splicing graphs
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| Crossreferences | ||||
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Expression Data
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| FlyBase-Curated Data | ||||
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Transcript and Protein data | Please see the FlyBase Gene Expression Report for details of gene expression from the literature. | |||
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Summary of Transcript Expression
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Stage Tissue/Position
Reference
pupal stage | mid-late
embryonic stage-adult stage
pupal stage | mid-late
embryonic stage | mid-late
embryonic stage | mid-late | ||||
| Marker for | ||||
| Subcellular Localization | ||||
| CV Term | ||||
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Summary of Polypeptide Expression
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Stage Tissue/Position
Reference
adult stage
myofibril | ||||
| Marker for | ||||
| Subcellular Localization | ||||
| CV Term | ||||
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Microarray Data
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Developmental timecourse, Costello et al., 2008 (Original data from Arbeitman et al., 2002) 
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External Data & Images
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| Linkouts | FLIGHT
- Cell culture data for RNAi and other high-throughput technologies
FlyAtlas
- Adult expression by tissue, using Affymetrix Dros2 array
GEO (NCBI)
- Gene expression data: microarray and other high-throughput technologies
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Alleles & Phenotypes
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Summary of Allele Phenotypes
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Lethality Allele | ||||
