Gene Dmel\fj

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General Information

Symbol

Dmel\fj

Species

D. melanogaster

Name

four-jointed

Annotation symbol

CG10917

Feature type

protein_coding_gene

FlyBase ID

FBgn0000658

Created / Updated

2003-12-01/2003-12-01

Genomic Location

Chromosome (arm)

Recombination map

2-81.5

Cytogenetic map

55C1-55C1

Sequence location

2R:14,120,268..14,123,819 [+]

Map ( GBrowse )

Summary Information

Automatically generated summary

See sections below for more information

The gene four-jointed is referred to in FlyBase by the symbol fj (CG10917, FBgn0000658). It has the cytological map location 55C1. Its sequence location is 2R:14120268..14123819. Its molecular function is unknown. It is involved in the biological processes: cell-cell signaling; imaginal disc growth; imaginal disc-derived wing vein specification; imaginal disc-derived leg joint morphogenesis; establishment of epithelial cell polarity; establishment of ommatidial polarity; establishment of planar polarity; imaginal disc development; proximal/distal pattern formation, imaginal disc. 43 alleles are reported. The phenotypes of these alleles are annotated with 33 unique terms, many of which group under: adult segment; peripheral nervous system; thoracic segment; adult mesothoracic segment; appendage segment; nervous system; adult; organ system; metatarsus; antennal segment. It has one annotated transcript and one annotated polypeptide.

External Summaries

Phenotypic Description from the Red Book (Lindsley & Zimm 1992)

Gene/Allele symbols may differ from current usage

fj: four jointed

fj: four jointed
Edith M. Wallace, unpublished.

Similar to d. Tarsi four instead of five jointed. Legs short and stocky, owing to failure of joint formation between second and third tarsal segments (Tokunaga and Gerhart, 1976, Genetics 83: s76). Femur, tibia, and tarsae foreshortened [Tokunaga and Gerhart, 1976; Mikuta, 1979, Genetika (Moscow) 15: 624-32]. Probability of joint failure proportional to degree of shortening of second tarsal segment (Tokunaga and Gerhart, 1976). Joint failure phenotype can extend into fj/+ tissue adjacent to fj/fj clones (Tokunaga and Gerhart, 1976). Leg chaetae show irregularities in the relative orientation of sockets and bracts (Held, Duarte, and Derakhshanian, 1986, Wilhelm Roux's Arch. Dev. Biol. 195: 145-57). Enhanced by ssa and ssaB [Villee, 1945, Genetics 30: 26-27; Mglinetz and Ivanov, 1976, Genetika (Moscow) 12: 87-94] and by pb [Kaurov, Ivanov, and Mglinetz, 1978, Genetika (Moscow) 14: 306-12]; also influenced by AntpNs (Mikuta and Mglinetz). fj eyD flies have but three tarsal joints (Postlethwait and Schneiderman, 1975, Ann. Rev. Genet. 7: 381-433). Development similar to that of dachs [Waddington, 1943, J. Genet. 45: 29-43 (fig.)]. Wings shorter and broader with crossveins conspicuously closer together; veins diverge at greater angle (Tokunaga, Michinomae, Sizemore, and Gerhart, 1978, Genetics 88: s98). Effect visible in pupal wing (Waddington, 1940, J. Genet. 41: 75-139). Eyes smaller, ellipsoid, coarse textured; head foreshortened. RK2.

Detailed Mapping Data

FlyBase Computed Cytological Location

Cytogenetic map

Evidence for location

55C1-55C1

Limits computationally determined from genome sequence between P{PZ}Hsf⁰³⁰⁹¹&P{EP}Dgp-1^EP731 and P{PZ}sbb⁰⁴⁵²⁵&P{EP}EP1081^EP1081

Experimentally Determined Cytological Location

Cytogenetic map

Notes

References

55C-55C

(Brodsky, 1996.1.9)

55B-55C

(determined by in situ hybridisation)

(Villano and Katz, 1995)

55C-55C

(Brodsky and Steller, 1995)

Experimentally Determined Recombination Data

Location

2-81.5

Left of (cM)

Right of (cM)

Notes

Molecular Map Data

Gene Order (in direction of increasing cytology)

References

Gene Order (overall orientation not stated)

References

Gene Model & Products

Please see the GBrowse view of Dmel\fj for information on other features

Comments on Gene Model

Transcript Data

Annotated Transcripts

Name

FlyBase ID

RefSeq ID

Length (nt)

Associated CDS (aa)

fj-RA

FBtr0086754

NM_058135

3552

583

Additional Transcript Data & Comments

Reported size (kB)

3.5 (northern blot)

(Brodsky and Steller, 1996)

3.7 (northern blot)

(Villano and Katz, 1995)

Comments

External Data

Crossreferences

Polypeptide Data

Annotated Polypeptides

Name

FlyBase ID

Predicted MW (kD)

Length (aa)

Theoretical pI

RefSeq ID

GenBank protein

fj-PA

FBpp0085933

65.5

583

8.74

NP_477483

AAF57724

Additional Polypeptide Data & Comments

Reported size (kD)

583 (aa); 66 (kD predicted)

(Villano and Katz, 1995)

583 (aa)

(Brodsky and Steller, 1996)

Comments

Rabbit antibody raised against amino acids 485-583. Rat antibody raised against amino acids 111-433.

(Strutt et al., 2004)

External Data

Linkouts

PANTHER - Protein classification by function, families, and pathways

•

FBgn0000658

Crossreferences

Sequences Consistent with the Gene Model

DDBJ /
EMBL /
GenBank

DNA sequence

Protein sequence

Name

P54360

UniProtKB/TrEMBL

Maps to

DS08374

(FlyBase, 1992-)

Does NOT map to

Identified with

RE18087

(BDGP Project Members, 2000-)

Mapped Features & Mutations

Please see GBrowse or insertion reports for information on insertions of transgenic constructs and features not listed here

Type

Symbol & Location

Additional Notes

References

deletion

fj[d1]
2R:14,119,189..14,123,189

comment=FBrf0086339: Deletion of approximately 4kb extending into fj transcribed region and removing at least the first 100 amino acids including a putative signal sequence. FBrf0123257: All but the last 57 amino acids of the coding region are deleted, with no remaining in frame methionines. Position of mutation on reference sequence inferred by FlyBase curator based on author statement.
evidence=experimental

(Brodsky and Steller, 1996, Zeidler et al., 1999)

rescue fragment

fj[+t24]
2R:14,103,333..14,127,698

comment=Position of restriction fragment on reference sequence inferred by FlyBase curator.
evidence=experimental
linked_to=SalI-SpeI_rfrag

(Strutt et al., 2004)

rescue fragment

fj[+t14]
2R:14,112,922..14,127,698

comment=Position of restriction fragment on reference sequence inferred by FlyBase curator.
evidence=experimental
linked_to=KpnI-SpeI_rfrag

(Strutt et al., 2004)

External Data

Linkouts

DEDB - Drosophila exon database: splicing graphs

•

11994

Crossreferences

Expression Data

FlyBase-Curated Data

Transcript and
Protein data

Please see the FlyBase Gene Expression Report for details of gene expression from the literature.

Summary of Transcript Expression

Stage

Tissue/Position

Reference

pupal stage

ventral thoracic disc | restricted

(Villano and Katz, 1995)

larval stage | third instar

dorsal mesothoracic disc | restricted

(Villano and Katz, 1995)

larval stage | third instar

outer optic anlagen

(Villano and Katz, 1995)

larval stage | third instar

lamina

(Brodsky and Steller, 1996)

larval stage | third instar

eye-antennal disc | gradient

(Brodsky and Steller, 1996)

larval stage | third instar

ventral thoracic disc

(Brodsky and Steller, 1996)

pupal stage

tibia

(Villano and Katz, 1995)

larval stage | third instar

dorsal mesothoracic disc

(Brodsky and Steller, 1996)

larval stage | third instar

ventral thoracic disc | restricted

(Villano and Katz, 1995)

larval stage | third instar

eye-antennal disc | restricted

(Villano and Katz, 1995)

Marker for

Subcellular Localization

CV Term

Summary of Polypeptide Expression

Stage

Tissue/Position

Reference

Marker for

Subcellular Localization

CV Term

External Data & Images

Linkouts

FLIGHT - Cell culture data for RNAi and other high-throughput technologies

•

FBgn0000658

FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array

•

FBgn0000658

GEO (NCBI) - Gene expression data: microarray and other high-throughput technologies

•

FBgn0000658

BDGP expression data

•

Show all BDGP original images, annotations and embryonic microarray data for fj

FlyExpress - Embryonic expression images (BDGP data)

•

Show all FlyExpress processed images for fj

Alleles & Phenotypes

Summary of Allele Phenotypes

Lethality

Allele

viable

lethal (with fj^d3)

fj^VG1

lethal (with fj^VG1)

fj^d3

lethal | recessive

fj^VG1

Other Phenotypes

Allele

visible

visible | recessive

visible | dominant

fj^VG1

visible | somatic clone, with Scer\GAL4^Act5C.PI

fj^Scer\UAS.cZa

visible, with Scer\GAL4^69B

fj^Scer\UAS.cZa

visible, with Scer\GAL4^dpp.blk1

fj^Scer\UAS.cZa

visible, with Scer\GAL4^ptc-559.1

fj^Scer\UAS.cZa

visible, with Scer\GAL4^en-e16E

fj^Scer\UAS.cZa

cell polarity defective | cell non-autonomous | somatic clone

fj^d1

cell polarity defective | cell non-autonomous | somatic clone, with Scer\GAL4^Ubx.PdC

fj^Scer\UAS.cZa

cell polarity defective | cell non-autonomous | somatic clone, with Scer\GAL4^αTub84B.PL

fj^Scer\UAS.cZa

cell polarity defective | somatic clone

fj^N7

tissue polarity defective

fj^d1

tissue polarity defective (with Df(2R)Pcl11B)

fj^d1

tissue polarity defective | cell autonomous | somatic clone

fj^d1

tissue polarity defective | cell non-autonomous | somatic clone

fj^d1

tissue polarity defective | cell non-autonomous | somatic clone, with fj^d1

fj^{t14.T:Hsap\GALNT3}

tissue polarity defective | cell non-autonomous | somatic clone, with Scer\GAL4^Act5C.PI

tissue polarity defective | cell non-autonomous | somatic clone, with fj^{t14.T:Hsap\GALNT3}

fj^d1

tissue polarity defective | somatic clone, with fj^d1

fj^{t14.T:Hsap\GALNT3}

tissue polarity defective | somatic clone, with fj^{t14.T:Hsap\GALNT3}

fj^d1

tissue polarity defective, with Scer\GAL4^ptc-559.1

fj^Scer\UAS.cZa

tissue polarity defective, with Scer\GAL4^salm-459.2

fj^Scer\UAS.cZa

tissue polarity defective, with Scer\GAL4^en-e16E

fj^Scer\UAS.cZa

tissue polarity defective, with Scer\GAL4^bi-omb-Gal4

tissue polarity defective, with Scer\GAL4^ap-md544

fj^Scer\UAS.cZa

tissue polarity defective, with Scer\GAL4^{Scer\FRT.Act5C}

fj^Scer\UAS.cZa

tissue polarity defective, with Scer\GAL4^bi-omb3-GAL4

fj^Scer\UAS.cZa

Phenotype manifest in

Allele

leg

tarsal segment

wing, with Scer\GAL4^bi-omb-Gal4

crossvein, with Scer\GAL4^bi-omb-Gal4

ommatidium, with Scer\GAL4^bi-omb-Gal4

eye | dorsal, with Scer\GAL4^bi-omb-Gal4

eye | ventral, with Scer\GAL4^bi-omb-Gal4

ommatidium | cell non-autonomous | somatic clone, with Scer\GAL4^Act5C.PI

fj^40e

ommatidium | somatic clone

photoreceptor cell R3 | somatic clone

fj^N7

photoreceptor cell R4 | ectopic | somatic clone

fj^N7

wing hair | posterior compartment, with Scer\GAL4^en-e16E

fj^Scer\UAS.cZa

leg, with Scer\GAL4^en-e16E

fj^Scer\UAS.cZa

wing hair, with Scer\GAL4^salm-459.2

fj^Scer\UAS.cZa

wing hair, with Scer\GAL4^ap-md544

fj^Scer\UAS.cZa

wing hair, with Scer\GAL4^ptc-559.1

fj^Scer\UAS.cZa

anterior crossvein, with Scer\GAL4^ptc-559.1

fj^Scer\UAS.cZa

wing hair | cell non-autonomous, with Scer\GAL4^ptc-559.1

fj^Scer\UAS.cZa

tarsal segment, with Scer\GAL4^ptc-559.1

fj^Scer\UAS.cZa

abdominal tergite | cell non-autonomous | somatic clone, with Scer\GAL4^Ubx.PdC

fj^Scer\UAS.cZa

adult cuticle | cell non-autonomous | somatic clone, with Scer\GAL4^Ubx.PdC

fj^Scer\UAS.cZa

microchaeta | cell non-autonomous | somatic clone, with Scer\GAL4^Ubx.PdC

fj^Scer\UAS.cZa

macrochaeta | cell non-autonomous | somatic clone, with Scer\GAL4^Ubx.PdC

fj^Scer\UAS.cZa

abdominal tergite | cell non-autonomous | somatic clone, with Scer\GAL4^αTub84B.PL

fj^Scer\UAS.cZa

adult cuticle | cell non-autonomous | somatic clone, with Scer\GAL4^αTub84B.PL

fj^Scer\UAS.cZa

microchaeta | cell non-autonomous | somatic clone, with Scer\GAL4^αTub84B.PL

fj^Scer\UAS.cZa

macrochaeta | cell non-autonomous | somatic clone, with Scer\GAL4^αTub84B.PL

fj^Scer\UAS.cZa

eye disc, with Scer\GAL4^bi-omb-Gal4

fj^Scer\UAS.cZa

eye, with Scer\GAL4^bi-omb-Gal4

fj^Scer\UAS.cZa

tarsal segment | somatic clone, with Scer\GAL4^Act5C.PI

fj^Scer\UAS.cZa

ommatidium, with Scer\GAL4^bi-omb3-GAL4

fj^Scer\UAS.cZa

wing hair, with Scer\GAL4^{Scer\FRT.Act5C}

fj^Scer\UAS.cZa

leg, with Scer\GAL4^{Scer\FRT.Act5C}

fj^Scer\UAS.cZa

tarsal segment, with Scer\GAL4^{Scer\FRT.Act5C}

fj^Scer\UAS.cZa

leg | distal, with Scer\GAL4^{Scer\FRT.Act5C}

fj^Scer\UAS.cZa

wing | distal, with Scer\GAL4^{Scer\FRT.Act5C}

fj^Scer\UAS.cZa

wing vein, with Scer\GAL4^{Scer\FRT.Act5C}

fj^Scer\UAS.cZa

posterior crossvein, with Scer\GAL4^{Scer\FRT.Act5C}

fj^Scer\UAS.cZa

anterior crossvein, with Scer\GAL4^{Scer\FRT.Act5C}

fj^Scer\UAS.cZa

wing vein L4, with Scer\GAL4^{Scer\FRT.Act5C}

fj^Scer\UAS.cZa

wing | anterior/posterior compartment boundary, with Scer\GAL4^{Scer\FRT.Act5C}

fj^Scer\UAS.cZa

eye, with Scer\GAL4^{Scer\FRT.Act5C}

fj^Scer\UAS.cZa

ommatidium, with Scer\GAL4^{Scer\FRT.Act5C}

fj^Scer\UAS.cZa

joint, with Scer\GAL4^69B

fj^Scer\UAS.cZa

tarsal segment 2, with Scer\GAL4^69B

fj^Scer\UAS.cZa

tarsal segment 3, with Scer\GAL4^69B

fj^Scer\UAS.cZa

tarsal segment, with Scer\GAL4^69B

fj^Scer\UAS.cZa

joint | ectopic, with Scer\GAL4^69B

fj^Scer\UAS.cZa

leg, with Scer\GAL4^dpp.blk1

fj^Scer\UAS.cZa

tarsal segment, with Scer\GAL4^dpp.blk1

fj^Scer\UAS.cZa

mesothoracic tergum

fj^VG1

tarsal segment (with fj^d3)

fj^VG1

ommatidium | cell non-autonomous | somatic clone, with fj^{t14.T:Hsap\GALNT3}

fj^d1

ommatidium | somatic clone, with fj^{t14.T:Hsap\GALNT3}

fj^d1

eye (with Df(2R)Pcl11B)

fj^d1

ommatidium (with Df(2R)Pcl11B)

fj^d1

eye | somatic clone

fj^d1

abdominal tergite | cell non-autonomous | somatic clone

fj^d1

adult cuticle | cell non-autonomous | somatic clone

fj^d1

microchaeta | cell non-autonomous | somatic clone

fj^d1

macrochaeta | cell non-autonomous | somatic clone

fj^d1

eye

wing hair

fj^d1

wing hair | cell autonomous | somatic clone

fj^d1

wing hair | cell non-autonomous | somatic clone

fj^d1

abdominal segment 2 & microchaeta

fj^d1

abdominal segment 2 & macrochaeta

fj^d1

wing | distal

fj^d1

wing vein | cell non-autonomous | somatic clone

fj^d1

wing vein | cell autonomous | somatic clone

fj^d1

wing vein | cell non-autonomous | ectopic | somatic clone

fj^d1

tarsal segment (with fj^VG1)

fj^d3

tarsal segment 4

fj^hs.PB

joint | ectopic

fj^hs.PB

wing vein | ectopic

fj^p1

abdomen

fj^p1

ommatidium | cell non-autonomous | somatic clone, with fj^d1

fj^{t14.T:Hsap\GALNT3}

ommatidium | somatic clone, with fj^d1

fj^{t14.T:Hsap\GALNT3}

crossvein

fj^{t14.T:Hsap\GALNT3}

coxa

fj^unspecified

arista

fj^unspecified

scutellum

fj^unspecified

antennal segment 3

fj^unspecified

abdominal tergite 2

fj^unspecified

Classical Alleles ( 23 )

For All Classical Alleles Show

Allele of fj	Class	Mutagen	Stocks	Known lesion
fj¹		spontaneous	6	--
fj²	amorph	Δ2-3	0	Yes
fj^333a		P-element activity	0	--
fj³	hypomorph	Δ2-3	0	Yes
fj^40e			0	--
fj⁴	hypomorph	Δ2-3	1	Yes
fj^9-II		P-element activity	1	Yes
fj^CB04634		P-element activity	0	Yes
fj^EP2183		P-element activity	1	--
fj^KG03419		P-element activity	1	--
fj^N7	hypomorph	P-element activity	1	Yes
fj^VG1		P-element activity	0	Yes
fj^d1	loss of function	Δ2-3	1	Yes
fj^d2		Δ2-3	0	Yes
fj^d3		Δ2-3	0	Yes
fj^e00145		piggyBac transposase	1	--
fj^p1		Δ2-3	0	Yes
fj^p2		Δ2-3	0	Yes
fj^p3		Δ2-3	0	Yes
fj^p4		Δ2-3	0	Yes
fj^p5		Δ2-3	0	Yes
fj^p6		Δ2-3	0	Yes
fj^unspecified			0	--

Alleles Carried on Transgenic Constructs ( 20 )

For All Alleles Carried on Transgenic Constructs Show