Gene Dmel\Fas1

General Information
Symbol	Dmel\Fas1	Species	D. melanogaster
Name	Fasciclin 1	Annotation symbol	CG6588
Feature type	protein_coding_gene	FlyBase ID	FBgn0000634
Created / Updated	2003-12-01/2008-06-17
Genomic Location
Chromosome (arm)	3R	Recombination map
Cytogenetic map	89D5-89D6	Sequence location	3R:12,450,948..12,465,493 [+]
Map ( GBrowse )
Summary Information
Automatically generated summary See sections below for more information	The gene Fasciclin 1 is referred to in FlyBase by the symbol Fas1 (CG6588, FBgn0000634). It has the cytological map location 89D5-89D6. Its sequence location is 3R:12450948..12465493. Its molecular function is described as cell adhesion molecule binding. It is involved in the biological processes: neuron recognition; axon guidance; calcium-independent cell-cell adhesion; homophilic cell adhesion; neuron adhesion. 22 alleles are reported. No phenotypic data is available. It has 6 annotated transcripts and 6 annotated polypeptides.
External Summaries

Phenotypic Description from the Red Book (Lindsley & Zimm 1992)
Gene/Allele symbols may differ from current usage	Fas1: Fasciclin I Expression confined to neurons. In 12-14 h embryos high levels of fasciclin I are detectable in two commissural axon bundles (fascicles) per segment, a thick one in the posterior commissure and a thin one in the anterior commissure. In addition high expression is seen in two clusters of neuronal cell bodies per hemisegment as well as in the intersegmental and segmental nerve axons. Finally, fasciclin I is detected on the surface of all peripheral neurons. Postulated to be involved in neuron recognition during growth cone guidance. Flies deficient in FasI function are fully viable and CNS development apparently is normal.
Detailed Mapping Data
FlyBase Computed Cytological Location
Cytogenetic map Evidence for location 89D5-89D6 Limits computationally determined from genome sequence between P{lacW}CSN5^L4032 and P{EP}MESK4^EP1015
Experimentally Determined Cytological Location
Cytogenetic map Notes References 89E1-89E2 (determined by in situ hybridisation) 89D (determined by in situ hybridisation) (Zinn et al., 1988)
Experimentally Determined Recombination Data
Location
Left of (cM)
Right of (cM)
Notes
Molecular Map Data
Gene Order (in direction of increasing cytology) References In direction of increasing cytology: Fas1+ nonA-l+ Cctγ+ CG14906- CG31217- CG31217- Ubx- bxd- Glut3+ abd-A- iab-4+ CG10349+ Abd-B- Actn3- Ahcy89E- (Martin et al., 1995) In direction of increasing cytology: Fas1? nonA-l? Cctγ? Ubx? (Walkley and Malik, 1996) Gene Order (overall orientation not stated) References
Gene Model & Products
Please see the GBrowse view of Dmel\Fas1 for information on other features
Comments on Gene Model

Transcript Data
Annotated Transcripts
Name FlyBase ID RefSeq ID Length (nt) Associated CDS (aa) Fas1-RA FBtr0083332 NM_169726 3067 649 Fas1-RB FBtr0083333 NM_169725 3076 652 Fas1-RC FBtr0083334 NM_169724 3085 655 Fas1-RD FBtr0112803 NM_001104344 3106 662 Fas1-RE FBtr0300036 3279 624 Fas1-RF FBtr0300037 3315 636
Additional Transcript Data & Comments
Reported size (kB)	5.2, 5.0, 4.2, 4.0, 3.2, 3.0 (northern blot) (McAllister et al., 1992) 3.0 (longest cDNA) (Zinn et al., 1988)
Comments	This minor transcript is expressed at high levels in Schneider 1 cells. (McAllister et al., 1992, McAllister et al., 1992, McAllister et al., 1992, McAllister et al., 1992) This major transcript is expressed at high levels in Schneider 1 cells. PCR analysis shows that Fas1 transcripts can vary by 3 or 6 amino acids through the inclusion or exclusion of two 9 bp microexons (exons 8 and 9). Type I transcripts contain neither microexon, type II transcripts contain exon 8, and type III transcripts contain both exone expressed in isolated 10-13 hr ventral nerve cords. Its expression pattern suggests that the 3.0 kb Fas1 transcript can exist in type I and type II forms. e expressed in isolated 10-13 hr ventral nerve cords. Its expression pattern suggests that the 3.0 kb Fas1 transcript can exist in type I and type II forms. (McAllister et al., 1992, McAllister et al., 1992) This major transcript is expressed at high levels in Schneider 1 cells. PCR analysis shows that Fas1 transcripts can vary by 3 or 6 amino acids through the inclusion or exclusion of two 9 bp microexons (exons 8 and 9). Type I transcripts contain neither microexon, type II transcripts contain exon 8, and type III transcripts contain both exons 8 and 9. Type I is dominant in embryogenesis, type II is expressed at 9-15 hours of embryogenesis, and type III is expressed at low levels in embryogenesis with a peak at 9-15 hours. All 3 types re expressed in isolated 10-13 hr ventral nerve cords. Its expression pattern suggests that the 5.0 kb Fas1 transcript can exist in type I form. (McAllister et al., 1992) The 3.0 and 5.0 kb major Fas1 transcripts, and the 3.2, 4.0, 4.2, and 5.2 kb minor Fas1 transcripts encode the same polypeptide, and differ in the length of the 3\\' untranslated region. A low level of heterogeneity within each transcript size class is seen, however, as a result of the use of two alternate micro-exons (exons 8 and 9), which give rise to polypeptides differing from the shortest Fas1 isoform by 3 or 6 amino acids. (McAllister et al., 1992)
External Data
Crossreferences
Polypeptide Data
Annotated Polypeptides
Name FlyBase ID Predicted MW (kD) Length (aa) Theoretical pI RefSeq ID GenBank protein Fas1-PA FBpp0082782 72.2 649 6.91 NP_732166 AAF55346 Fas1-PB FBpp0082783 72.6 652 7.03 NP_732165 AAN13715 Fas1-PC FBpp0082784 73.0 655 7.03 NP_732164 AAN13714 Fas1-PD FBpp0111715 73.7 662 7.53 NP_001097814 ABW08688 Fas1-PE FBpp0289313 69.5 624 6.91 Fas1-PF FBpp0289314 70.9 636 6.63
Additional Polypeptide Data & Comments
Reported size (kD)	72 (kD observed) (Hortsch and Goodman, 1990) 652 (aa); 70 (kD predicted) (Zinn et al., 1988)
Comments	Fas1 is an extrinsic membrane protein, composed of 4 homologous domains of 150 aa each. (Zinn et al., 1988) In Schneider 1 cells, Fas1 was found to be tightly associated with the lipid bilayer by a phosphotidylinositol lipid moiety. The proportion of membrane bound to soluble Fas1 was assayed during embryogenesis. The amount of membrane-bound, phosphatidylinositol-linked Fas1 was higher from blastoderm to early gastrulation stages. During germ band extension and retraction, the amount of soluble Fas1 was 3 times higher than membrane-bound Fas1. Later, as Fas1 expression became restricted to the nervous system and a few other tissues, the phosphatidylinositol linked form was once again the predominant form. (Hortsch and Goodman, 1990)
External Data
Linkouts	PANTHER - Protein classification by function, families, and pathways • FBgn0000634
Crossreferences	InterPro domains - A database of protein families, domains, and functional sites • Beta-Ig-H3/fasciclin (IPR000782) PDB - Protein Data Bank. An information portal to biological macromolecular structures • 1O70
Sequences Consistent with the Gene Model
DDBJ / EMBL / GenBank	DNA sequence Protein sequence Name AA202408 AA438670 AA540394 AA540643 AA698495 AA735574 AA941423 AA950971 AE003714 AAF55346 AE003714 AAN13714 AE003714 AAN13715 AI062634 AI062941 AI114005 AI114025 AI134041 AI134747 AI292848 AI293265 AI387389 AI404748 AI405633 AI513459 AI516067 AW942737 AX093939 AY118531 AAM49900 BI164307 BI215550 BI216383 BI227761 BI228668 BI235780 BI236938 BI242784 BI243757 BI362000 BI366197 BI373674 BI376047 BI484218 BI568918 BI569932 BI575534 BI576878 BI579013 BI579941 BI585262 BI589261 BI589339 BI590065 BI590278 BI626344 BI629262 BP541553 BP553092 BP553093 BP555143 BP556645 BP556646 BP558634 BT015956 CK086521 CK086522 CK657881 CL528259 CL705807 CO177119 CO177723 CO178085 CO178357 CO179288 CO179390 CO186453 CO186770 CO187673 CO187983 CO188915 CO189623 CO190145 CO190623 CO190736 CO196520 CO262144 CO262618 CO262993 CO264322 CO267128 CO267215 CO269052 CO269550 CO271221 CO271532 CO272548 CO273270 CO273656 CO274947 CO275334 CO277953 CO280041 CO280118 CO280452 CO280541 CO284481 CO286140 CO286430 CO286726 CO286828 CO287899 CO288138 CO288407 CO289853 CO289923 CO291466 CO291571 CO291707 CO295008 CO296248 CO297064 CO298328 CO299223 CO300881 CO301787 CO304442 CO304643 CO305737 CO306795 CO307921 CO308435 CO310397 CO310535 CO312042 CO312635 CO313516 CO314348 CO315071 CO315099 CO315305 CO318402 CO318444 CO319554 CO320764 CO320824 CO321966 CO323247 CO323340 CO329391 CO332689 CO332874 CO333601 CO342302 CO343715 CO344078 CO344314 CO345409 CZ468154 CZ478776 CZ479771 CZ479772 EC057907 EC058173 EC058996 EC061466 EC063199 EC063511 EC065535 EC066792 EC069005 EC069148 EC069341 EC069385 EC069497 EC070336 EC070571 EC072132 EC073375 EC075379 EC075804 EC076738 EC078271 EC078760 EC080147 EC080887 EC082071 EC082146 EC083365 EC083409 EC083565 EC084720 EC084769