HomeGene Dmel\E(z)
| General Information | |||||||||||||||||||||||||||||||||||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Symbol | Dmel\E(z) | Species | D. melanogaster | ||||||||||||||||||||||||||||||||||||||||||
| Name | Enhancer of zeste | Annotation symbol | CG6502 | ||||||||||||||||||||||||||||||||||||||||||
| Feature type | protein_coding_gene | FlyBase ID | FBgn0000629 | ||||||||||||||||||||||||||||||||||||||||||
| Created / Updated | 2003-12-01/2003-12-01 | ||||||||||||||||||||||||||||||||||||||||||||
| Genomic Location | |||||||||||||||||||||||||||||||||||||||||||||
| Chromosome (arm) | 3L | Recombination map | 3-34.0 | ||||||||||||||||||||||||||||||||||||||||||
| Cytogenetic map | 67E5-67E5 | Sequence location | 3L:10,627,668..10,631,230 [-] | ||||||||||||||||||||||||||||||||||||||||||
| Map ( GBrowse ) | 
| ||||||||||||||||||||||||||||||||||||||||||||
Summary Information
| |||||||||||||||||||||||||||||||||||||||||||||
|
Automatically generated summary
See sections below for more information | The gene Enhancer of zeste is referred to in FlyBase by the symbol E(z) (CG6502, FBgn0000629). It has the cytological map location 67E5. Its sequence location is 3L:10627668..10631230. Its molecular function is described as: histone lysine N-methyltransferase activity (H3-K27 specific); DNA binding; histone lysine N-methyltransferase activity (H3-K9 specific); histone methyltransferase activity; protein binding. It is involved in the biological processes: histone methylation; pheromone metabolic process; chromatin silencing; syncytial blastoderm mitotic cell cycle; axon guidance; muscle development; dendrite morphogenesis; neurogenesis. 99 alleles are reported. The phenotypes of these alleles are annotated with 50 unique terms, many of which group under: adult segment; larval abdominal segment; imaginal precursor; thoracic segment; peripheral nervous system; anatomical structure; adult mesothoracic segment; nervous system; adult; larval thorax. It has 2 annotated transcripts and 2 annotated polypeptides. | ||||||||||||||||||||||||||||||||||||||||||||
| External Summaries | |||||||||||||||||||||||||||||||||||||||||||||
|
Phenotypic Description from the Red Book (Lindsley & Zimm 1992)
| |||||||||||||||||||||||||||||||||||||||||||||
| Gene/Allele symbols may differ from current usage | E(z): Enhancer of zeste
Locus named after original gain-of-function allele
E(z)1 (Kalisch and Rasmuson); subsequently designated polycombeotic (pco) (by Phillips and Shearn) based on phenotype of
lethal homozygotes. Loss of function alleles recovered as (a)
recessive lethal mutations (b) reversions of E(z)1 and (c)
reversions of the antimorphic allele, E(z)59. Reduction of
E(z)+ activity leads to suppression of the z eye color,
whereas gain-of-function alleles are dominant enhancers of
zeste eye color [i.e., z w+/Y; E(z)1/+ males have brownish
eyes as do z w+/z+ w+; E(z)1/+ females]. E(z)59 an
antimorphic allele, is a dominant suppressor of z [i.e. z w+;
E(z)59/+ females have orange eyes]. Hemizygosity for E(z)+
produces a very mild suppression of the z eye color. No
effects on eye color in z+ or za backgrounds, and effects on
eye color not specific to a particular w allele. Reduction of
E(z)+ activity also allows ectopic expression of the segment
identity genes of the Antennapedia and bithorax gene complexes, resulting in homeotic transformations. This latter
effect defines E(z) as a Polycomb-group locus. E(z)61
displays temperature-sensititive suppression of z eye color
and homeotic phenotypes. At 22, z wis/Y; E(z)61/Df(3L)E(z)-males have orange eyes and no homeotic transformations. At
29, such males have wild-type red eyes and die as pharate
adults with strong homeotic transformations of the mesothoracic and metathoracic legs toward the prothoracic state.
Embryos produced by E(z)61 homozygous females at 29C die with
homeotic transformations of most segments toward the eighth
abdominal segment. Even two copies of paternally contributed
E(z)+ does not rescue viability of these embryos. Complete
lack of zygotically produced E(z)+ results in early pupal
lethality and small imaginal disks. Larval brain squashes from
individuals homozygous for an amorphic allele reveal a very
low mitotic index; metaphase chromosomes irregularly condensed
and fragmented (Gatti and Baker, 1989, Genes Dev. 3: 438-53).
| ||||||||||||||||||||||||||||||||||||||||||||
|
Detailed Mapping Data
| |||||||||||||||||||||||||||||||||||||||||||||
| FlyBase Computed Cytological Location | |||||||||||||||||||||||||||||||||||||||||||||
Cytogenetic map Evidence for location 67E5-67E5
Limits computationally determined from genome sequence between P{lacW}l(3)L0539L0539&P{PZ}Dhh1rL562 and P{PZ}simj01814
| |||||||||||||||||||||||||||||||||||||||||||||
| Experimentally Determined Cytological Location | |||||||||||||||||||||||||||||||||||||||||||||
Cytogenetic map Notes References 67E3-67E4 (determined by in situ hybridisation)
| |||||||||||||||||||||||||||||||||||||||||||||
| Experimentally Determined Recombination Data | |||||||||||||||||||||||||||||||||||||||||||||
| Location | 3-30.9 +/- 0.7 3-30.9 3-34.0 3-34.25 +/- 0.5 | ||||||||||||||||||||||||||||||||||||||||||||
| Left of (cM) | |||||||||||||||||||||||||||||||||||||||||||||
| Right of (cM) | |||||||||||||||||||||||||||||||||||||||||||||
| Notes | Genetic map position contradicts cytological map position. | ||||||||||||||||||||||||||||||||||||||||||||
| Molecular Map Data | |||||||||||||||||||||||||||||||||||||||||||||
Gene Order (in direction of increasing cytology)
References Gene Order (overall orientation not stated) References | |||||||||||||||||||||||||||||||||||||||||||||
|
Gene Model & Products
| |||||||||||||||||||||||||||||||||||||||||||||
Please see the
GBrowse view of
Dmel\E(z)
for information on other features
| |||||||||||||||||||||||||||||||||||||||||||||
| Comments on Gene Model | |||||||||||||||||||||||||||||||||||||||||||||
|
Transcript Data
| |||||||||||||||||||||||||||||||||||||||||||||
| Annotated Transcripts | |||||||||||||||||||||||||||||||||||||||||||||
Name FlyBase ID RefSeq ID Length (nt) Associated CDS (aa) FBtr0273338
2470
760 | |||||||||||||||||||||||||||||||||||||||||||||
| Additional Transcript Data & Comments | |||||||||||||||||||||||||||||||||||||||||||||
| Reported size (kB) | 2.5 (northern blot) 3.3, 2.5 (northern blot) | ||||||||||||||||||||||||||||||||||||||||||||
| Comments | |||||||||||||||||||||||||||||||||||||||||||||
| External Data | |||||||||||||||||||||||||||||||||||||||||||||
| Crossreferences | |||||||||||||||||||||||||||||||||||||||||||||
|
Polypeptide Data
| |||||||||||||||||||||||||||||||||||||||||||||
| Annotated Polypeptides | |||||||||||||||||||||||||||||||||||||||||||||
Name FlyBase ID
Predicted MW (kD)
Length (aa)
Theoretical pI
RefSeq ID
GenBank protein
FBpp0271846
86.9
760
6.29
| |||||||||||||||||||||||||||||||||||||||||||||
| Additional Polypeptide Data & Comments | |||||||||||||||||||||||||||||||||||||||||||||
| Reported size (kD) | 760 (aa); 87 (kD predicted) 89 (kD observed) | ||||||||||||||||||||||||||||||||||||||||||||
| Comments | E(z) protein was found to bind to 44 identified sites along the polytene chromosomes. | ||||||||||||||||||||||||||||||||||||||||||||
| External Data | |||||||||||||||||||||||||||||||||||||||||||||
| Linkouts | PANTHER
- Protein classification by function, families, and pathways
| ||||||||||||||||||||||||||||||||||||||||||||
| Crossreferences | InterPro
domains - A database of protein families, domains, and functional sites
• SANT, DNA-binding (IPR001005)
SET (IPR001214)
| ||||||||||||||||||||||||||||||||||||||||||||
|
Sequences Consistent with the Gene Model
| |||||||||||||||||||||||||||||||||||||||||||||
| DDBJ
/
EMBL / GenBank | DNA sequence Protein sequence Name | ||||||||||||||||||||||||||||||||||||||||||||
| UniProtKB/Swiss-Prot | |||||||||||||||||||||||||||||||||||||||||||||
| UniProtKB/TrEMBL | |||||||||||||||||||||||||||||||||||||||||||||
| Maps to | |||||||||||||||||||||||||||||||||||||||||||||
| Does NOT map to | |||||||||||||||||||||||||||||||||||||||||||||
| Identified with | |||||||||||||||||||||||||||||||||||||||||||||
|
Mapped Features & Mutations
| |||||||||||||||||||||||||||||||||||||||||||||
Please see
GBrowse
or insertion reports for information on insertions of transgenic
constructs and features not listed here
Type Symbol & Location Additional Notes References aberration junction T(2;3)E(z)[65].bk2 3L:10,629,855..10,630,148 comment=Break 2 of T(2;3)E(z)[65] maps to a HindII-BamHI fragment in E(z). Break 1 maps to hay. Position of restriction fragment on reference sequence inferred by FlyBase curator. evidence=experimental linked_to=HindIII-BamHI_rfrag aberration junction T(2;3)E(z)[65].bk1 3L:10,624,701..10,625,172 comment=Aberration breakpoint mapped to a BamHI-EcoRI restriction fragment; position of restriction fragment on reference sequence inferred by FlyBase curator. evidence=experimental linked_to=BamHI-EcoRI_rfrag deletion Df(3L)E(z)[66].bk2 3L:10,625,826..10,629,860 comment=Df(3L)E(z)[66] is a 1.5 kb deletion that falls within a BamHI-EcoRI fragment. evidence=experimental linked_to=BamHI-EcoRI_rfrag insertion site H{}E(z)[60] 3L:10,628,520..10,628,522 comment=The insertion site maps to codon 523; E(z)[60] encodes a C-terminally truncated protein that lacks amino acids 524 to 760. The orientation of the insertion was not reported. evidence=experimental point mutation E(z)[63] 3L:10,630,859..10,630,859 evidence=experimental na_change=G10630859A pr_change=M1I|E(z)-PA reported_na_change=G106A reported_pr_change=M1I point mutation E(z)[28] 3L:10,629,121..10,629,121 evidence=experimental na_change=G10629121A pr_change=C363Y|E(z)-PA reported_na_change=G1191A reported_pr_change=C363Y point mutation E(z)[2434] 3L:10,628,941..10,628,941 evidence=experimental na_change=C10628941T pr_change=Q404@|E(z)-PA reported_na_change=C?T reported_pr_change=Q404@ point mutation E(z)[32] 3L:10,628,455..10,628,455 evidence=experimental na_change=G10628455A pr_change=C545Y|E(z)-PA reported_na_change=G1737A reported_pr_change=C545Y point mutation E(z)[1025] 3L:10,628,432..10,628,432 evidence=experimental na_change=C10628432T pr_change=Q553@|E(z)-PA reported_na_change=C?T reported_pr_change=Q553@ point mutation E(z)[61] 3L:10,628,281..10,628,281 evidence=experimental na_change=G10628281A pr_change=C603Y|E(z)-PA reported_na_change=G1191A reported_pr_change=C603Y point mutation E(z)[E4G.2] 3L:10,628,225..10,628,225 comment=E(z) protein is truncated at the beginning of the SET domain. The site of the nucleotide substitution in the mutant was inferred by FlyBase based on the reported amino acid change. evidence=experimental na_change=C10628225T pr_change=R622@|E(z)-PA reported_pr_change=R622@ point mutation E(z)[731] 3L:10,628,107..10,628,107 comment=TGG to TGA evidence=experimental pr_change=W638@|E(z)-PA reported_na_change=G?A reported_pr_change=W638@ point mutation E(z)[328] 3L:10,628,107..10,628,107 comment=TGG to TGA. evidence=experimental pr_change=W638@|E(z)-PA reported_na_change=G?A reported_pr_change=W638@ point mutation E(z)[Trm] 3L:10,627,799..10,627,799 comment=Differs from E(z)[TrmTG] in having a second silent mutation. evidence=experimental na_change=G10627799A pr_change=R741K|E(z)-PA reported_na_change=G2325A reported_pr_change=R741K point mutation E(z)[TrmTG] 3L:10,627,799..10,627,799 evidence=experimental na_change=G10627799A pr_change=R741K|E(z)-PA reported_na_change=G2325A reported_pr_change=R741K rescue fragment E(z)[+t8.8] 3L:10,625,826..10,635,464 comment=Approximate endpoints of rescue fragment described as extending from coordinates -3.6kb to +5.2kb on the genomic map. The endpoints fall very close to EcoRI restriction fragments on the map in Figure 1 of FBrf0055584. evidence=experimental linked_to=EcoRI-EcoRI_rfrag | |||||||||||||||||||||||||||||||||||||||||||||
|
External Data
| |||||||||||||||||||||||||||||||||||||||||||||
| Linkouts | DEDB
- Drosophila exon database: splicing graphs
• | ||||||||||||||||||||||||||||||||||||||||||||
| Crossreferences | |||||||||||||||||||||||||||||||||||||||||||||
|
Expression Data
| |||||||||||||||||||||||||||||||||||||||||||||
| FlyBase-Curated Data | |||||||||||||||||||||||||||||||||||||||||||||
|
Transcript and Protein data | Please see the FlyBase Gene Expression Report for details of gene expression from the literature. | ||||||||||||||||||||||||||||||||||||||||||||
|
Summary of Transcript Expression
| |||||||||||||||||||||||||||||||||||||||||||||
Stage Tissue/Position
Reference
embryonic stage-adult stage
adult stage | male
larval stage,pupal stage | |||||||||||||||||||||||||||||||||||||||||||||
| Marker for | |||||||||||||||||||||||||||||||||||||||||||||
| Subcellular Localization | |||||||||||||||||||||||||||||||||||||||||||||
| CV Term | |||||||||||||||||||||||||||||||||||||||||||||
|
Summary of Polypeptide Expression
| |||||||||||||||||||||||||||||||||||||||||||||
Stage Tissue/Position
Reference
larval stage | third instar
embryonic stage | |||||||||||||||||||||||||||||||||||||||||||||
| Marker for | |||||||||||||||||||||||||||||||||||||||||||||
| Subcellular Localization | |||||||||||||||||||||||||||||||||||||||||||||
| CV Term | nucleus
polytene chromosome | ||||||||||||||||||||||||||||||||||||||||||||
|
Microarray Data
|
|||||||||||||||||||||||||||||||||||||||||||||
|
Developmental timecourse, Costello et al., 2008 (Original data from Arbeitman et al., 2002) 
| |||||||||||||||||||||||||||||||||||||||||||||
|
External Data & Images
| |||||||||||||||||||||||||||||||||||||||||||||
| Linkouts | FLIGHT
- Cell culture data for RNAi and other high-throughput technologies
FlyAtlas
- Adult expression by tissue, using Affymetrix Dros2 array
GEO (NCBI)
- Gene expression data: microarray and other high-throughput technologies
FlyExpress
- Embryonic expression images (BDGP data)
| ||||||||||||||||||||||||||||||||||||||||||||
|
Alleles & Phenotypes
| |||||||||||||||||||||||||||||||||||||||||||||
|
Summary of Allele Phenotypes
| |||||||||||||||||||||||||||||||||||||||||||||
Lethality Allele
Other Phenotypes Allele Sterility Allele Phenotype manifest in Allele larval brain & mitosis & nuclear chromosome mesothoracic leg & sex comb | ectopic | |||||||||||||||||||||||||||||||||||||||||||||
|
Classical Alleles
( 91 ) | |||||||||||||||||||||||||||||||||||||||||||||
| For All Classical Alleles Show | |||||||||||||||||||||||||||||||||||||||||||||
| |||||||||||||||||||||||||||||||||||||||||||||
Stage(s)
1-3
Stage(s)
4-6
Stage(s)
7-8
Stage(s)
9-10
Stage(s)
11-12