A Database of Drosophila Genes & Genomes

FB2008_07, released August 8, 2008
 

Gene Dmel\E(Pc)

General Information
SymbolDmel\E(Pc)SpeciesD. melanogaster
NameEnhancer of PolycombAnnotation symbolCG7776
Feature typeprotein_coding_geneFlyBase IDFBgn0000581
Created / Updated2003-12-02/2003-12-02
Genomic Location
Chromosome (arm)2RRecombination map2-61.9
Cytogenetic map47F13-47F14Sequence location2R:7,339,722..7,351,535 [+]
Map ( GBrowse ) detailed view
hide Summary Information
Automatically generated summary

See sections below for more information
The gene Enhancer of Polycomb is referred to in FlyBase by the symbol E(Pc) (CG7776, FBgn0000581). It has the cytological map location 47F13-47F14. Its sequence location is 2R:7339722..7351535. Its molecular function is unknown. It is involved in the biological process establishment and/or maintenance of chromatin architecture. 11 alleles are reported. No phenotypic data is available. It has 2 annotated transcripts and 2 annotated polypeptides.

External Summaries

hide Phenotypic Description from the Red Book (Lindsley & Zimm 1992)
Gene/Allele symbols may differ from current usage
E(Pc): Enhancer of Polycomb (R. Denell)
Although E(Pc) heterozygotes are cuticularly normal as larvae and adults, the mutation acts zygotically as a dominant enhancer of the adult homoeotic syndrome of flies heterozygous for Polycomb mutations or Df(3L)Pc-MK. The locus is haplo-abnormal, as heterozygous deficiencies of the E(Pc) locus have an equivalent effect. Heterozygosity for E(Pc) or a deficiency also enhances the adult homoeotic syndrome of Polycomblike mutation or deficiency heterozygotes, and renders the normally recessive mutations esc and l(4)29 slightly pseudodominant, but has no effect on phenotypes associated with AntpNs, Antp73b, AntpScx, AntpEfW15, ScrMsc, Cbx, Ubx, bx34e or abx bx3 pbx heterozygotes. E(Pc) is a recessive lethal mutation; homozygotes and hemizygotes die as late embryos or larvae, which appear cuticularly normal. E(Pc) has dominant maternal as well as zygotic effect on the severity of the embryonic homoeotic syndrome of Pc3 or Df(3L)Pc-MK homozygotes. Zygotic homozygosity for E(Pc) also enhances the embryonic effects of PclW4, PclW5, and PclW6, and E(Pc); l(4)29 doubly homozygous embryos from heterozygous mothers show incomplete head involution, presumably due to a cryptic homoeotic effect.
hide Detailed Mapping Data
FlyBase Computed Cytological Location
Cytogenetic map
Evidence for location
47F13-47F14  
Limits computationally determined from genome sequence between P{lacW}Tapδk17005&P{lacW}k05103 and P{lacW}l(2)k13403k13403&P{lacW}Egmk14708  
Experimentally Determined Cytological Location
Cytogenetic map
Notes
References
48A-48A
 
(Stankunas, 1998.7.22)
48A-48A
 
(Kassis, 1994)
48A-48A
 
(Drees et al., 1987)
Experimentally Determined Recombination Data
Location
2-61.9
 
2-
(Drees et al., 1987)
Left of (cM)
Right of (cM)
Notes
Molecular Map Data
Gene Order (in direction of increasing cytology)
References
In direction of increasing cytology: Drip? anon-48Ab? E(Pc)? inv?
(Sinclair et al., 1998)
In direction of increasing cytology: E(Pc)+ inv+
(Stankunas et al., 1998)
Gene Order (overall orientation not stated)
References
Overall orientation not stated: Drip? anon-48Ab? E(Pc)? inv? en? tou? anon-48Ae? anon-48Af? anon-48Ag? anon-48Ah? anon-48Ai? anon-48Aj?
(Drees et al., 1987)
hide Gene Model & Products
Please see the GBrowse view of Dmel\E(Pc) for information on other features
detailed view FBtr0089862 FBtr0114575 FBtr0088139 FBtr0088140 FBpp0087238 FBpp0087239 FBti0035346 FBti0035841 FBti0046766 FBti0034355 FBti0056926 FBti0007641 FBti0026388 FBti0029581 FBti0029563
Comments on Gene Model
DGC clone appears problematic (GH14582): incomplete CDS
hide Transcript Data
Annotated Transcripts
Name
FlyBase ID
RefSeq ID
Length (nt)
Associated CDS (aa)
E(Pc)-RA
FBtr0088139
 NM_078974
  9380
  2023
E(Pc)-RB
FBtr0088140
 NM_165836
  9307
  2023
Additional Transcript Data & Comments
Reported size (kB)
8.5, 5.2 (northern blot)
(Stankunas et al., 1998)
Comments
External Data
Crossreferences
hide Polypeptide Data
Annotated Polypeptides
Name
FlyBase ID
Predicted MW (kD)
Length (aa)
Theoretical pI
RefSeq ID
GenBank protein
E(Pc)-PA  
FBpp0087238  
220.9  
2023  
6.04  
  NP_523698
  AAF58641
E(Pc)-PB  
FBpp0087239  
220.9  
2023  
6.04  
  NP_725054
  AAM68743
Additional Polypeptide Data & Comments
Reported size (kD)
2023 (aa); 220 (kD predicted)
(Stankunas et al., 1998)
Comments
External Data
Linkouts
PANTHER - Protein classification by function, families, and pathways
•
Crossreferences
hide Sequences Consistent with the Gene Model
DDBJ /
EMBL /
GenBank
DNA sequence
Protein sequence
Name
AE003826
AAF58641
 
AE003826
AAM68743
 
AF079764
AAC64271
 
AI107868
 
AI108420
 
AI238526
 
AI293808
 
AI402022
 
AQ026451
 
AW940560
 
AY059438
AAL13344
 
AY059438
AAL13344
 
AY119519
AAM50173
 
BF488365
 
BF489589
 
CO177834
 
CO178255
 
CO272643
 
CO280876
 
CO281439
 
CO288236
 
CO298697
 
CO300321
 
CO303555
 
CO305101
 
CO306323
 
CO309903
 
CO328765
 
CO331651
 
CO343456
 
CZ476892
 
EC063093
 
EC072303
 
EC077439
 
EC082970
 
EC084853
 
EC085389
 
EC088685
 
EC194893
 
EC195800
 
EC199400
 
EC203185
 
EC209254
 
EC216153
 
EC218933
 
EC225013
 
EC239654
 
EC242029
 
EC249593
 
EC255530
 
EC257356
 
EC260578
 
EL876187
 
EL876188
 
EL876256
 
EL876257
 
EL876330
 
EL876331
 
 
UniProtKB/Swiss-Prot
    UniProtKB/TrEMBL
    Maps to
    Does NOT map to
    Identified with
    (Stapleton, 2001.10.8)
    (BDGP Project Members, 2000-)
    hide Mapped Features & Mutations
    Please see GBrowse or insertion reports for information on insertions of transgenic constructs and features not listed here
    Type
    Symbol & Location
    Additional Notes
    References
    hide External Data
    Linkouts
    DEDB - Drosophila exon database: splicing graphs
    •
    Crossreferences
    hide Expression Data
    FlyBase-Curated Data
    Transcript and
    Protein data     		Please see the FlyBase Gene Expression Report for details of gene expression from the literature.
    hide Summary of Transcript Expression
    Stage
    Tissue/Position
    Reference
     embryonic stage-adult stage
     
    (Stankunas et al., 1998)
     embryonic stage-adult stage
     
    (Stankunas et al., 1998)
    Marker for
      Subcellular Localization
      CV Term
      hide Summary of Polypeptide Expression
      Stage
      Tissue/Position
      Reference
       embryonic stage
       
      (Stankunas et al., 1998)
       embryonic stage
       embryonic central nervous system
      (Stankunas et al., 1998)
      Marker for
        Subcellular Localization
        CV Term
        hide Microarray Data
        Developmental timecourse, Costello et al., 2008 (Original data from Arbeitman et al., 2002)
        Untitled Document detailed view FBtr0089862 FBtr0114575 FBtr0088139 FBtr0088140
        AI238526
        AI107868
        hide External Data & Images
        Linkouts
        FLIGHT - Cell culture data for RNAi and other high-throughput technologies
        •
        FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array
        •
        GEO (NCBI) - Gene expression data: microarray and other high-throughput technologies
        •
        BDGP expression data
        •
        Show all BDGP original images, annotations and embryonic microarray data for E(Pc)
        FlyExpress - Embryonic expression images (BDGP data)
        •
        Show all FlyExpress processed images for E(Pc)
        hide Alleles & Phenotypes
        hide Summary of Allele Phenotypes
        Lethality
        Allele
        lethal | recessive
        Other Phenotypes
        Allele
        suppressor of variegation | dominant, with Dp(1;Y)BSCV
        suppressor of variegation | dominant, with In(1)wm4
        suppressor of variegation | dominant, with In(2R)bwVDe2
        suppressor of variegation | dominant, with T(2;3)SbV
        suppressor of variegation, with In(1)wm4
        suppressor of variegation, with T(2;3)SbV
        radiation resistant | dominant
        Phenotype manifest in
        Allele
        hide Classical Alleles ( 10 )
        For All Classical Alleles Show

        Allele of E(Pc)ClassMutagenStocksKnown lesion
        E(Pc)14 --
        E(Pc)20 Yes
        E(Pc)D4
          		1 --
          E(Pc)EP6082 --
          E(Pc)d097061 --
          E(Pc)rv100 Yes
          E(Pc)rv40 Yes
          E(Pc)rv90 Yes
          E(Pc)unspecifiedamorph, gain of function
            		0 --
            E(Pc)w3
              		1 --
              hide Alleles Carried on Transgenic Constructs ( 1 )
              For All Alleles Carried on Transgenic Constructs Show

              Allele of E(Pc)ClassMutagenStocksKnown lesion
              E(Pc)GD122822 Yes
              hide Aneuploid Aberrations
              Useful deficiency
               
              Useful duplication
               
              Disrupted in
              (Christensen and Cook, 2006.8.29)
              (Sinclair et al., 1998, Sato et al., 1983, Cheng et al., 1994)
              (Sinclair et al., 1998, Sato et al., 1983)
              (Sinclair et al., 1998, Sato et al., 1983, Soto et al., 1995)
              (Sinclair et al., 1998, Sato et al., 1983)
              (Sato et al., 1983)
              (Sinclair et al., 1998)
              (Sinclair et al., 1998)
              Not disrupted in
              (Sinclair et al., 1998)
              (Sinclair et al., 1998)
              (Sinclair et al., 1998)
              hide Transgenic Constructs & Insertions
              Transgenic Constructs
              Type of construct
              Name
              Expression data
              UAS construct
              P{GD12282}
              NA
              Insertions
              Type of insertions
              Name
              Expression data
              miscellaneous insertions
              P{wen.4}E(Pc)2
              NA
              insertion of enhancer trap
              P{lacW}l(2)SH0139SH0139
              No
              insertion of mobile activating element
              P{EP}E(Pc)EP608
              NA
              P{XP}E(Pc)d09706
              NA
              hide Related Comments
              Please look at the allele reports for the complete phenotype data
              Genetic interactions have been demonstrated between esc, Asx, E(Pc), Pcl, E(z) and sxc. Most duplications of Pc-group genes neither exhibit anterior transformations nor suppress the extra sex comb phenotype of Pc-group mutations, suggesting that not all Pc-group genes behave as predicted by the mass action model.
              (Campbell et al., 1995)
              Mutations in E(Pc) suppress position effect variegation.
              (Sinclair et al., 1998)
              hide Gene Ontology: Function, Process & Cellular Component ( 5 )
              hide Molecular Function
              CV term
              Evidence
              References
              hide Biological Process
              CV term
              Evidence
              References
              establishment and/or maintenance of chromatin architecture
              inferred from mutant phenotype
              (Sinclair et al., 1998)
              hide Cellular Component
              CV term
              Evidence
              References
              chromatin
              non-traceable author statement
              (Stankunas, 1998.7.22)
              intercalary heterochromatin
              non-traceable author statement
              (Zhimulev and Belyaeva, 2003)
              polytene chromosome
              inferred from direct assay
              (Stankunas et al., 1998)
              NOT
              polytene chromosome chromocenter
              inferred from direct assay
              (Stankunas et al., 1998)
              hide Sequence Ontology: Class of Gene
              protein_coding_gene
               
              nuclear_gene
               
              hide Interactions & Pathways
              hide Summary of Genetic Interactions
              Interacts with
              Please look at the allele data for full details of the genetic interactions
              E(Pc) allele
              Gene
              References
              E(Pc)1
              w
              (Netter et al., 1998)
              ash1
              (Gildea et al., 2000)
              brm
              (Gildea et al., 2000)
              E(z)
              (Bajusz et al., 2001)
              Pc
              (Sinclair et al., 1998)
              esc
              (Lindsley and Zimm, 1992)
              pho
              (Lindsley and Zimm, 1992)
              CycE
              (Brumby et al., 2002)
              trx
              (Gildea et al., 2000)
              E(Pc)2
              ash1
              (Gildea et al., 2000)
              Pc
              (Sinclair et al., 1998, Stankunas et al., 1998)
              E(Pc)rv10
              Pc
              (Sinclair et al., 1998)
              E(Pc)rv4
              Pc
              (Sinclair et al., 1998)
              E(Pc)rv9
              Pc
              (Sinclair et al., 1998)
              E(Pc)unspecified
              w
              (Bloyer et al., 2003)
              unspecified
              ph-p
              (Fauvarque et al., 1995)
              ara
              (Netter et al., 1998)
              Pc
              (Lindsley and Zimm, 1992, Campbell et al., 1995)
              esc
               
              Scm
              (Campbell et al., 1995)
              Pcl
              (Lindsley and Zimm, 1992, Campbell et al., 1995)
              pho
               
              Sce
              (Campbell et al., 1995)
              ph-PRE
              (Bloyer et al., 2003)
              E(Pc)a
               
              hide External Data
              Linkouts
              BioGRID - Interaction data, including yeast 2-hybrid and genetic interactions
              •
              Drosophila PIMRider - The Drosophila Protein Interaction map
              •
              hide Orthologs
              Genome-wide drosophilid orthologs
              Curated drosophilid orthologs
               
              Linkouts
              InParanoid orthologs - Eukaryotic orthologs
              •
              hide Functional Complementation between Species
              hide Inter-Species Misexpression Data
              Produces phenotype in
              Produces NO phenotype in
              hide Stocks & Reagents
              hide Stocks Listed in FlyBase ( 12 )
              Szeged
              -
              P{EP}E(Pc)EP608
              -
              P{lacW}l(2)SH0139SH0139
              Harvard
              -
              P{XP}E(Pc)d09706
              VDRC
              v35268
              w1118; P{GD12282}v35268
              v35271
              w1118; P{GD12282}v35271/CyO
              Kyoto
              106875
              esc5 E(Pc)1/SM5
              106808
              cn1 E(Pc)1 bw1/SM5
              Bloomington
              3142
              esc5 E(Pc)1/SM5
              17286
              w1118; P{EP}E(Pc)EP608
              3056
              cn1 E(Pc)1 bw1/SM5
              9396
              y1 w67c23; E(Pc)w3/CyO
              9398
              y1 w67c23; E(Pc)D4/CyO
              hide Genomic Clones ( 1 )
              hide cDNA Clones ( 23 )
              Please Note
              This section lists cDNAs and ESTs that fall within the genomic extent of the gene model, which may include cDNAs and ESTs of genes within introns, or of overlapping genes. Please see GBrowse for alignment of the cDNAs and ESTs to the gene model.
              cDNA Clones, Fully Sequenced
              BDGP DGC clones
              Other clones
              cDNA Clones, End Sequenced (ESTs)
              BDGP DGC clones
              Other clones