• Home
• Tools
  • BLAST
  • GBrowse
  • QueryBuilder
  • TermLink
  • ImageBrowse
  • Interactions Browser
  • Apollo
  • CytoSearch
  • QuickSearch
  • Batch Download
  • Coordinate Converter
  • Google™ FlyBase
  • Find A Person
  • Update An Address
  • Add A New Person
• Files
  • Downloads:
  • Precomputed files
  • Archived data
  • Releases FTP
  • Genomes FTP
  • Lists:
  • Transposons (Dmel)
  • Transposons (other)
  • Vectors & Constructs
  • Anatomical Terms
  • Map Conversion
• Species
  • Phylogeny
  • Synteny Table
  • Drosophilidae
  • Abbreviations
• Documents
  • About FlyBase
  • Reference Manual:
  • Sections
  • Contents
  • Nomenclature
  • Release Notes
• Resources
  • All resources
  • Interactive Fly
  • Textpresso for Fly
  • AAA site
  • BDGP
  • DHGP
  • DGRC
  • Model Organisms
  • Stock Collections:
  • Bloomington
  • DrosDel
  • Ehime
  • Exelixis (Harvard)
  • GDP (Baylor)
  • Kyoto
  • NIG-Fly
  • Szeged
  • Tucson
  • VDRC
• News
  • News
  • FlyBase Forum
  • FlyBase Positions
  • Meetings
  • Courses
  • Fly Board
  • White papers
  • Funding
  • bionet.dros
• Help
  • Google™ FlyBase
  • Site Map
  • FAQs
  • Report help
  • New to Flies
  • Pers. comm.
  • Author guidelines
  • Known Problems
  • Contact FlyBase
• Archives
  • Prior Home Page
  • R4.3 BLAST
  • R4.3 GBrowse
  • Older Archives

Gene Dmel\eg

General Information
Symbol	Dmel\eg	Species	D. melanogaster
Name	eagle	Annotation symbol	CG7383
Feature type	protein_coding_gene	FlyBase ID	FBgn0000560
Created / Updated	2003-12-01/2003-12-01
Genomic Location
Chromosome (arm)	3L	Recombination map	3-47.3
Cytogenetic map	78F2-78F3	Sequence location	3L:21,801,803..21,811,081 [+]
Map ( GBrowse )	Decorated FastA GenBankGFF Gene region Extended Gene region CDSIntronsExonsTranslationsTranscripts3' UTR5' UTR
Summary Information
Automatically generated summary See sections below for more information	The gene eagle is referred to in FlyBase by the symbol eg (CG7383, FBgn0000560). It has the cytological map location 78F2-78F3. Its sequence location is 3L:21801803..21811081. Its molecular function is described as: ligand-dependent nuclear receptor activity; transcription factor activity; zinc ion binding; sequence-specific DNA binding. It is involved in the biological processes: central nervous system development; regulation of transcription, DNA-dependent. 18 alleles are reported. The phenotypes of these alleles are annotated with: wing; serotonin neuron; EW1 neuron; wing sensillum; embryonic dorsal epidermis; dorsal closure embryo; neuroblast NB7-3. It has 2 annotated transcripts and 2 annotated polypeptides.
External Summaries
Phenotypic Description from the Red Book (Lindsley & Zimm 1992)
Gene/Allele symbols may differ from current usage	eg: eagle Wings extended. Hairs on thorax somewhat disarranged. Dark pattern on thorax. Viability varies among alleles; eg2 females sterile. RK2. Egon: Embryonic gonad Expression detected in embryonic gonad but not in other tissues or at other stages of development.
Detailed Mapping Data
FlyBase Computed Cytological Location
Cytogenetic map Evidence for location 78F2-78F3   Limits computationally determined from genome sequence between P{lacW}l(3)j1B10j1B10 and P{lacW}l(3)j2C4j2C4
Experimentally Determined Cytological Location
Cytogenetic map Notes References 79A3-79A4 (determined by in situ hybridisation)   (Hay et al., 1997) 79B-79B (determined by in situ hybridisation)   (Rothe et al., 1989)
Experimentally Determined Recombination Data
Location	3-47.3 (Duncan and Kaufman, 1975)
Left of (cM)
Right of (cM)	Pc (Puro and Nygren, 1975)
Notes
Molecular Map Data
Gene Order (in direction of increasing cytology) References Gene Order (overall orientation not stated) References Overall orientation not stated: eg+ anon-79Ba+ (Higashijima et al., 1996)
Gene Model & Products
Please see the GBrowse view of Dmel\eg for information on other features
Comments on Gene Model
Transcript Data
Annotated Transcripts
Name FlyBase ID RefSeq ID Length (nt) Associated CDS (aa) eg-RA FBtr0078438  NM_079482   2181   373 eg-RB FBtr0078437  NM_168938   1884   373
Additional Transcript Data & Comments
Reported size (kB)	2.2, 1.9 (compiled cDNA) (Higashijima et al., 1996)
Comments
External Data
Crossreferences
Polypeptide Data
Annotated Polypeptides
Name FlyBase ID Predicted MW (kD) Length (aa) Theoretical pI RefSeq ID GenBank protein eg-PA   FBpp0078092   39.6   373   8.68     NP_524206   AAF51783 eg-PB   FBpp0078091   39.6   373   8.68     NP_730689   AAN12188
Additional Polypeptide Data & Comments
Reported size (kD)	373 (aa) (Higashijima et al., 1996)
Comments
External Data
Linkouts	PANTHER - Protein classification by function, families, and pathways • FBgn0000560
Crossreferences	InterPro domains - A database of protein families, domains, and functional sites • Vitamin D receptor (IPR000324) Zinc finger, nuclear hormone receptor-type (IPR001628) Zinc finger, NHR/GATA-type (IPR013088)
Sequences Consistent with the Gene Model
DDBJ / EMBL / GenBank	DNA sequence Protein sequence Name AE003595 AAF51783   AE003595 AAN12188   AI063830   BF503355   BT028846   D43634 BAA07742   D43635 BAA07743   GH03923   X16631 CAA34626
UniProtKB/Swiss-Prot	P15370
UniProtKB/TrEMBL
Maps to
Does NOT map to
Identified with	D1309 (BDGP Project Members, 1994-1999)
Mapped Features & Mutations
Please see GBrowse or insertion reports for information on insertions of transgenic constructs and features not listed here Type Symbol & Location Additional Notes References
External Data
Linkouts	DEDB - Drosophila exon database: splicing graphs • 6057
Crossreferences
Expression Data
FlyBase-Curated Data
Transcript and Protein data	Please see the FlyBase Gene Expression Report for details of gene expression from the literature.
Summary of Transcript Expression
Stage Tissue/Position Reference  embryonic stage | stage 11-12  neuroblast NB3-3 (Higashijima et al., 1996)  embryonic stage | stage 11-12  neuroblast NB7-3 (Higashijima et al., 1996)  embryonic stage | stage 11-12  neuroblast NB6-4 (Higashijima et al., 1996)  embryonic stage | stage 11-12  neuroblast NB2-4 (Higashijima et al., 1996)  embryonic stage | stage 14  dorsal fold (Higashijima et al., 1996)  embryonic stage | stage 11-12  embryonic central nervous system | presumptive | restricted (Higashijima et al., 1996)  embryonic stage | stage 11-12  neuroblasts of ventral nervous system | subset (Higashijima et al., 1996)
Marker for
Subcellular Localization
CV Term
Summary of Polypeptide Expression
Stage Tissue/Position Reference
Marker for
Subcellular Localization
CV Term
Microarray Data
	Developmental timecourse, Costello et al., 2008 (Original data from Arbeitman et al., 2002) AI063830
External Data & Images
Linkouts	FLIGHT - Cell culture data for RNAi and other high-throughput technologies • FBgn0000560 FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array • FBgn0000560 GEO (NCBI) - Gene expression data: microarray and other high-throughput technologies • FBgn0000560
Alleles & Phenotypes
Summary of Allele Phenotypes
Lethality Allele viable eg57c egMz360 egP179 egP289 lethal | recessive eg18B lethal | pupal stage | recessive egP289 egT6 Other Phenotypes Allele visible eg1 eg2 eg55B eg57c eg84B eg89A egP179 visible | recessive egMz360 egP289 egspy visible | dominant egP289 egT6 visible, with Scer\GAL4kni.L2 egScer\UAS.cDa locomotor behavior defective egP289 egT6 flightless egP289 egT6 Sterility Allele fertile eg57c Phenotype manifest in Allele wing eg1 eg2 eg55B eg57c eg84B eg89A egMz360 egP179 egP289 egspy serotonin neuron eg18B eg225A egMz360 egP289 egT6 EW1 neuron eg18B eg225A egMz360 egP289 egT6 serotonin neuron & larva eg18B egP289 serotonin neuron & ventral nerve cord egMz360 ventral nerve cord & serotonin neuron egP289 egT6 neuroblast NB7-3 egP289 wing sensillum | ectopic, with Scer\GAL4kni.L2 egScer\UAS.cDa serotonin neuron | ectopic, with Scer\GAL4sca-537.4 egScer\UAS.cDa embryonic dorsal epidermis, with Scer\GAL4pnr-MD237 egScer\UAS.cDa dorsal closure embryo, with Scer\GAL4pnr-MD237 egScer\UAS.cDa
Classical Alleles ( 15 )
For All Classical Alleles Show
Allele of eg Class Mutagen Stocks Known lesion eg18B Δ2-3 P-element activity 0 Yes eg1 4 -- eg225A P-element activity 0 Yes eg28 P-element activity 0 Yes eg2 5 -- eg55B amorph Δ2-3 0 -- eg57c spontaneous 0 -- eg84B Δ2-3 0 -- eg89A Δ2-3 0 -- egMz360 Δ2-3 P-element activity 1 Yes egP179 P-element activity 0 -- egP289 loss of function, hypomorph P-element activity 0 Yes egT6 hypomorph Δ2-3 P-element activity 0 Yes egspy X ray 0 -- egunspecified 0 --
Alleles Carried on Transgenic Constructs ( 3 )
For All Alleles Carried on Transgenic Constructs Show
Allele of eg Class Mutagen Stocks Known lesion egGD1429 in vitro construct | RNAi in vitro construct | regulatory fusion 3 Yes egGMR.PH in vitro construct | regulatory fusion 1 Yes egScer\UAS.cDa in vitro construct | regulatory fusion 0 Yes
Aneuploid Aberrations
Useful deficiency	Df(3L)78E2-3;79E
Disrupted in	Df(3L)BSC450 (Christensen et al., 2008.4.15) T(2;3)egspy (Puro and Arajarvi, 1969, Puro, 1982)
Transgenic Constructs & Insertions
Transgenic Constructs
	Type of construct Name Expression data reporter construct P{eg-kinesin-lacZ} Yes UAS construct P{GD1429} NA P{UAS-eg.D} NA characterization construct P{GMR-eagle} NA
Insertions
	Type of insertions Name Expression data miscellaneous insertions P{5'GawB}eg18B NA P{if-lacZ.L}egT6 NA insertion of enhancer trap binary system P{GawB}egMz360 Yes insertion of enhancer trap P{lArB}egP289 Yes P{lacZ-un3}egP179 Yes insertion of mobile activating element P{Mae-GMR.E}eg28 NA
Related Comments
	Please look at the allele reports for the complete phenotype data Egon first isolated from genomic library using a kni zinc-finger probe. Expression detected in embryonic gonad but not in other tissues or at other stages of development.   eg is isolated and characterised, eg RNA expression is restricted to 4 neuroblasts and a part of their putative progeny. (Higashijima et al., 1996) Loss of function eg mutations produce an unusual differential phenotype with respect to the sister serotonin cells. Hypomorphic alleles can produce viable adults that have a dramatic reduction in the number of serotonin-producing neurons. (Lundell and Hirsh, 1998) Mutant allele fails to complement a QTL affecting male mating behaviour. (Moehring and Mackay, 2004)
Gene Ontology: Function, Process & Cellular Component ( 7 )
Molecular Function
CV term Evidence References
ligand-dependent nuclear receptor activity inferred from sequence or structural similarity with EMBL:U09504 (FlyBase, 1992-) non-traceable author statement (Swiss-Prot Project Members, 1990.4.1) non-traceable author statement (Maglich et al., 2001)
sequence-specific DNA binding inferred from electronic annotation with InterPro:IPR001628 (FlyBase Curators et al., 2004-)
transcription factor activity inferred from sequence or structural similarity with EMBL:U09504 (FlyBase, 1992-)
zinc ion binding inferred from electronic annotation with InterPro:IPR001628, InterPro:IPR013088 (FlyBase Curators et al., 2004-)
Biological Process
CV term Evidence References
central nervous system development inferred from mutant phenotype (Dittrich et al., 1997)
regulation of transcription, DNA-dependent inferred from electronic annotation with InterPro:IPR000324, InterPro:IPR001628, InterPro:IPR013088 (FlyBase Curators et al., 2004-)
Cellular Component
CV term Evidence References
nucleus inferred from sequence or structural similarity with EMBL:U09504 (FlyBase, 1992-) non-traceable author statement (Swiss-Prot Project Members, 1990.4.1)
Sequence Ontology: Class of Gene
	protein_coding_gene   nuclear_gene
Interactions & Pathways
Summary of Genetic Interactions
Interacts with	Please look at the allele data for full details of the genetic interactions eg allele Gene References eg18B kay (Gritzan et al., 2002) rl (Gritzan et al., 2002) eg28 Ras85D (Hay et al., 1997) egGMR.PH Ras85D (Hay et al., 1997) egMz360 lea (Couch et al., 2004) egScer\UAS.cDa lea (Couch et al., 2004) kni (Lunde et al., 2003) kay (Gritzan et al., 2002) puc (Gritzan et al., 2002) unspecified hkb (Dittrich et al., 1997)
External Data
Linkouts	BioGRID - Interaction data, including yeast 2-hybrid and genetic interactions • 65668
Orthologs
Genome-wide drosophilid orthologs	Dana\GF25170 Dere\GG16225 Dgri\GH16373 Dmoj\GI11566 Dper\GL24862 Dpse\GA20312 Dsim\GD15001 Dvir\GJ11245 Dwil\GK17344 Dyak\GE19793
Curated drosophilid orthologs	Dpse\GA20312
Linkouts
Functional Complementation between Species
Inter-Species Misexpression Data
Produces phenotype in
Produces NO phenotype in
Stocks & Reagents
Stocks Listed in FlyBase ( 14 )
Bloomington	501 eg2 502 eg2/In(3LR)Cx 8758 w*; P{GawB}egMz360 500 eg1/TM3, Sb1 Ser1 5784 w*; P{GMR-eagle}l(3)1010/TM3, Sb1 1707 eg1 rn3 pp Ubxbx-1 sr1 es ca1/TM2 1683 st1 in1 kniri-1 eg2 Ki1 pp es/TM3, Sb1 Ser1
Kyoto	105942 eg2 105941 eg1/TM3, Sb1 Ser1
VDRC	v7155 w1118; P{GD1429}v7155 v7157 w1118; P{GD1429}v7157 v21762 w1118; P{GD1429}v21762
	More stocks available...
Genomic Clones ( 2 )
	BACR15K24 BACR22O03
cDNA Clones ( 2 )
	Please Note This section lists cDNAs and ESTs that fall within the genomic extent of the gene model, which may include cDNAs and ESTs of genes within introns, or of overlapping genes. Please see GBrowse for alignment of the cDNAs and ESTs to the gene model.
cDNA Clones, Fully Sequenced
BDGP DGC clones
Other clones
cDNA Clones, End Sequenced (ESTs)
BDGP DGC clones
Other clones	AT19322 GH03923
RNAi & Array Information
Affy Oligo
Linkouts	DRSC - RNAi screening (Harvard): high-throughput cell culture data and design • FBgn0000560 GenomeRNAi - RNAi phenotypes (Heidelberg): high-throughput cell culture data • FBgn0000560
Antibody Information
	monoclonal antibody (Couch et al., 2004)
Other Information
Discoverer
Etymology
Identification
Position Effect Variegation Data
Relationship to Other Genes
Source for database identity of
Source for database merge of	Source for merge of: eg egon (Higashijima et al., 1996)
Additional comments
Comments About Role
	eg and hkb act in concert to determine serotonergic cell fate in the developing central nervous system. (Dittrich et al., 1997) eg is essential for specifying the fate of serotonin neurons. eg is necessary for the maintenance of en and zfh2 expression in the serotonin neurons. (Lundell and Hirsh, 1998)
Comments About Molecular Function
Other Comments
	Mutant individuals display spread wings. (Duncan and Kaufman, 1975) Evolutionary history for nuclear receptor genes, in which gene duplication events and swapping between domains of different origins took place, is studied. (Laudet et al., 1992)
External Crossreferences & Linkouts
Sequence Crossreferences
	RefSeq (Transcripts) • NM_168938 NM_079482 RefSeq (Proteins) • NP_730689 NP_524206 DDBJ / EMBL / GenBank • AE003595 AAF51783 AAN12188 D43634 BAA07742 D43635 BAA07743 X16631 CAA34626 UniProtKB/Swiss-Prot • P15370
Other Crossreferences
	InterPro domains - A database of protein families, domains, and functional sites • Vitamin D receptor (IPR000324) Zinc finger, nuclear hormone receptor-type (IPR001628) Zinc finger, NHR/GATA-type (IPR013088)
Linkouts
	BioGRID - Interaction data, including yeast 2-hybrid and genetic interactions • 65668 DEDB - Drosophila exon database: splicing graphs • 6057 DRSC - RNAi screening (Harvard): high-throughput cell culture data and design • FBgn0000560 FLIGHT - Cell culture data for RNAi and other high-throughput technologies • FBgn0000560 FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array • FBgn0000560 FlyMine - Integrated genomics database for Drosophila, Anopheles, and C.elegans • FBgn0000560 GEO (NCBI) - Gene expression data: microarray and other high-throughput technologies • FBgn0000560 GenomeRNAi - RNAi phenotypes (Heidelberg): high-throughput cell culture data • FBgn0000560 Interactive Fly - A cyberspace guide to Drosophila development and metazoan evolution • /neural/eagle1.htm PANTHER - Protein classification by function, families, and pathways • FBgn0000560 University of Chicago Dev. Expression • FBgn0000560
Synonyms & Secondary IDs ( 16 )
Reported As
Symbol Synonym	CG7383   eag (Broadus et al., 1995) eg (Buescher et al., 2006, Jung et al., 2007, Christensen et al., 2008.4.15) Eg (Rogulja-Ortmann et al., 2007) egon (Thummel, 1995, Henrich and Brown, 1995, Gerwin et al., 1994, Oro et al., 1992, Segraves, 1991, Henrich et al., 1990, ) EGON (Laudet et al., 1992) Egon   NR0A3 (Nuclear Receptors Nomenclature Committee, 1999) spy (Puro and Arajarvi, 1969, ) T1 (Higashijima et al., 1996)
Name Synonym	eagle (Saigo, 1994.11.26, Saigo, 1994.11.26, Girard et al., 2006) Eagle (Cheesman et al., 2004) embryonic gonad (Oro et al., 1992) Embryonic gonad   spready   T1 transcription unit (Higashijima et al., 1996)
Secondary FlyBase IDs
	FBgn0000563
References ( 79 )
Generate a list of	All references relating to this gene ( 79 )
List References by type	Research paper  ( 50 ) Supplementary material  ( 1 ) Review  ( 6 ) Personal communication to FlyBase  ( 3 ) Abstract  ( 10 ) FlyBase analysis  ( 1 ) DNA/RNA sequence record  ( 2 ) Protein sequence record  ( 1 ) Computer file  ( 1 ) Curated genome annotation  ( 1 ) Automatic genome annotation  ( 1 ) Book  ( 2 )
Recent research papers ( 4 )
	Rogulja-Ortmann et al., 2007, Development 134(1): 105--116 Programmed cell death in the embryonic central nervous system of Drosophila melanogaster. [FBrf0194301] Buescher et al., 2006, Dev. Biol. 292(2): 418--429 Functions of the segment polarity genes midline and H15 in Drosophila melanogaster neurogenesis. [FBrf0190096] Girard et al., 2006, Dev. Biol. 299(2): 530--542 Chromatin immunoprecipitation reveals a novel role for the Drosophila SoxNeuro transcription factor in axonal patterning. [FBrf0194303] Mettler et al., 2006, Development 133(3)