Gene Dmel\EcR

General Information
Symbol	Dmel\EcR	Species	D. melanogaster
Name	Ecdysone receptor	Annotation symbol	CG1765
Feature type	protein_coding_gene	FlyBase ID	FBgn0000546
Created / Updated	2003-12-01/2003-12-01
Genomic Location
Chromosome (arm)	2R	Recombination map
Cytogenetic map	42A9-42A12	Sequence location	2R:1,978,285..2,056,589 [-]
Map ( GBrowse )
Summary Information
Automatically generated summary See sections below for more information	The gene Ecdysone receptor is referred to in FlyBase by the symbol EcR (CG1765, FBgn0000546). It has the cytological map location 42A9-42A12. Its sequence location is 2R:1978285..2056589. Its molecular function is described as: ecdysteroid hormone receptor activity; ligand-dependent nuclear receptor activity; protein binding; RNA polymerase I transcription factor activity; DNA binding; steroid binding; zinc ion binding; sequence-specific DNA binding; transcription factor activity. It is involved in the biological processes described with 36 unique terms, many of which group under: anatomical structure development; gamete generation; organ development; organ morphogenesis; regulation of metabolic process; spermatid differentiation; neuron differentiation; muscle development; embryonic development via the syncytial blastoderm; central nervous system development; regulation of developmental process. 119 alleles are reported. The phenotypes of these alleles are annotated with 63 unique terms, many of which group under: organ system; adult segment; imaginal precursor; nervous system; peripheral nervous system; anatomical structure; cuticle; adult mesothoracic segment; cephalopharyngeal skeleton; thoracic segment. It has 5 annotated transcripts and 5 annotated polypeptides.
External Summaries

Detailed Mapping Data
FlyBase Computed Cytological Location
Cytogenetic map Evidence for location 42A9-42A12 Limits computationally determined from genome sequence between P{lacW}l(2)k09848^k09848&P{EP}EP407 and P{lacW}geminin^k14019&P{PZ}Adf1⁰¹³⁴⁹
Experimentally Determined Cytological Location
Cytogenetic map Notes References 42A10-42A12 (determined by in situ hybridisation) 42A1--19 (determined by in situ hybridisation) (Spradling et al., 1999) 42A1-42A19 42A1--2 42A10--12 42A10--16 (BDGP Project Members, 1994-1999) 42A-42A (determined by in situ hybridisation) (Koelle et al., 1991)
Experimentally Determined Recombination Data
Location
Left of (cM)
Right of (cM)
Notes
Molecular Map Data
Gene Order (in direction of increasing cytology) References Gene Order (overall orientation not stated) References
Gene Model & Products
Please see the GBrowse view of Dmel\EcR for information on other features
Comments on Gene Model

Transcript Data
Annotated Transcripts
Name FlyBase ID RefSeq ID Length (nt) Associated CDS (aa) EcR-RA FBtr0086008 NM_165461 4744 849 EcR-RB FBtr0086011 NM_165465 5586 878 EcR-RC FBtr0086012 NM_165464 4620 669 EcR-RD FBtr0086009 NM_165462 4826 849 EcR-RE FBtr0086010 NM_165463 4872 849
Additional Transcript Data & Comments
Reported size (kB)	6, 5, 4 (northern blot) (Talbot et al., 1993) 6.0 (northern blot) (Koelle et al., 1991)
Comments
External Data
Crossreferences
Polypeptide Data
Annotated Polypeptides
Name FlyBase ID Predicted MW (kD) Length (aa) Theoretical pI RefSeq ID GenBank protein EcR-PA FBpp0085349 91.2 849 7.71 NP_724456 AAF57278 EcR-PB FBpp0085352 93.9 878 6.88 NP_724460 AAF57280 EcR-PC FBpp0085353 73.3 669 6.76 NP_724459 AAM68347 EcR-PD FBpp0085350 91.2 849 7.71 NP_724457 AAG22334 EcR-PE FBpp0085351 91.2 849 7.71 NP_724458 AAM68346
Additional Polypeptide Data & Comments
Reported size (kD)	878, 849, 669 (aa); 105, 80 (kD) (Talbot et al., 1993) 878 (aa); 105 (kD observed) (Koelle et al., 1991)
Comments	Three EcR isoforms exist that differ in their N-terminal sequences. They have 652 C-terminal amino acids in common that contain the DNA- and hormone-binding domains. All were shown to function as ecdysone receptors in S2 cells. Monoclonal antibodies have been produced that specifically recognize two of the three EcR isoforms (all but EcR-B2 protein). Monoclonal antibodies have also been produced against the region common to all of the EcR proteins. (Talbot et al., 1993) Three EcR isoforms exist that differ in their N-terminal sequences. They have 652 C-terminal amino acids in common that contain the DNA- and hormone-binding domains. All were shown to function as ecdysone receptors in S2 cells. Monoclonal antibodies have been produced that specifically recognize two of the three EcR isoforms (all but EcR-B2). Monoclonal antibodies have also been produced against the region common to all of the EcR proteins. (Talbot et al., 1993, Talbot et al., 1993) alternatively spliced exon (FlyBase, 1992-) Monoclonal antibody was generated against amino acids 67-878 of this protein. Polyclonal antibody was generated against amino acids 335-447 of this protein. EcR protein expressed in Drosophila Schneider 2 cells is able to bind the ecdysone analog iodoponasterone A, and binding activity is lost by treatment with EcR antibody. Embryonic extracts also show ecdysone analog binding which is removable by antibody treatment. EcR protein is able to specifically bind ecdysone response element (EcRE) DNA. (Koelle et al., 1991)
External Data
Linkouts	PANTHER - Protein classification by function, families, and pathways • FBgn0000546
Crossreferences	InterPro domains - A database of protein families, domains, and functional sites • Nuclear hormone receptor, ligand-binding, core (IPR000536) Zinc finger, nuclear hormone receptor-type (IPR001628) Steroid hormone receptor (IPR001723) Ecdysteroid receptor (IPR003069) Nuclear hormone receptor, ligand-binding (IPR008946) Zinc finger, NHR/GATA-type (IPR013088) TRANSFAC - Eukaryotic transcription factors, their genomic binding sites, and DNA-binding profiles • R03988 T00217
Sequences Consistent with the Gene Model
DDBJ / EMBL / GenBank	DNA sequence Protein sequence Name AA201657 AA202510 AA246305 AA263370 AA391360 AA438743 AA538642 AA539503 AA540854 AA567664 AA735619 AA941320 AA941361 AA978434 AA979330 AE003784 AAF57278 AE003784 AAF57280 AE003784 AAG22334 AE003784 AAM68346 AE003784 AAM68347 AI063285 AI258616 AI457009 AI511659 AI513045 AI514995 AI520411 AI520584 AI544194 AQ025786 AQ026415 AQ073646 AX407008 AY058575 AAL13804 AY075461 AAL68274 BE976426 BH011418 BH759480 BI163162 BI166834 BI167732 BI170398 BI215745 BI230982 BI237603 BI238123 BI241640 BI356066 BI373487 BI485877 BI577728 BT012469 BT015234 AAT94463 BZ286306 CA804850 CL525863 CO184318 CO190212 CO267419 CO280751 CO295210 CO301393 CO303536 CO306702 CO308056 CO313402 CO317115 CO318554 CO333058 CZ467670 CZ467758 CZ468804 CZ468960 CZ468983 CZ471893 CZ476085 CZ476400 CZ478924 CZ481520 CZ481521 CZ483792 CZ483793 CZ484679 CZ484779 CZ484780 CZ486449 CZ486853 CZ486854 CZ490259 EC062759 EC064183 EC064186 EC064325 EC064950 EC065396 EC066872 EC068743 EC069530 EC072629 EC080519 EC084092 EC085238 EC085371 EC195365 EC195394 EC196738 EC199382 EC203697 EC208354 EC210512 EC219502 EC219734 EC226068 EC227509 EC228274 EC229960 EC231133 EC231946 EC237962 EC238696 EC247382 EC248267 EC248505 EC249093 EC249920 EC255511 M74078 AAA28498 S63761 AAB27496 S63762

UniProtKB/Swiss-Prot	P34021
UniProtKB/TrEMBL	A4UZ51

Maps to
Does NOT map to
Identified with	LD17664 (Spradling et al., 1999) LD40781 LD26915 RE16656 RE33854 RE06878 (BDGP Project Members, 2000-) D1333 (BDGP Project Members, 1994-1999)
Mapped Features & Mutations
Please see GBrowse or insertion reports for information on insertions of transgenic constructs and features not listed here Type Symbol & Location Additional Notes References
External Data
Linkouts	DEDB - Drosophila exon database: splicing graphs • 11582
Crossreferences
Expression Data
FlyBase-Curated Data
Transcript and Protein data	Please see the FlyBase Gene Expression Report for details of gene expression from the literature.
Summary of Transcript Expression
Stage Tissue/Position Reference embryonic stage \| late (Talbot et al., 1993) embryonic stage \| late (Talbot et al., 1993) larval stage \| second instar (Talbot et al., 1993) pupal stage \| early (Talbot et al., 1993) larval stage-adult stage (Koelle et al., 1991) pupal stage \| mid (Talbot et al., 1993) embryonic stage \| mid (Talbot et al., 1993) embryonic stage \| >=3 hr (Koelle et al., 1991) larval stage \| first instar (Talbot et al., 1993) pupal stage (Talbot et al., 1993) larval stage \| third instar \| late (Talbot et al., 1993) embryonic stage \| mid (Talbot et al., 1993) larval stage \| third instar (Talbot et al., 1993) embryonic stage \| 0-3 hr (Talbot et al., 1993)
Marker for

Subcellular Localization
CV Term
Summary of Polypeptide Expression
Stage Tissue/Position Reference prepupal stage ventral thoracic disc (Talbot et al., 1993) adult stage, oogenesis stage follicle cell (Carney and Bender, 2000) prepupal stage epidermis (Talbot et al., 1993) larval stage \| first instar (Talbot et al., 1993) prepupal stage eye-antennal disc (Talbot et al., 1993) prepupal stage embryonic/larval trachea (Talbot et al., 1993) larval stage \| first instar (Koelle et al., 1991) prepupal stage imaginal disc (Talbot et al., 1993) prepupal stage embryonic/larval midgut (Talbot et al., 1993) larval stage \| third instar \| late (Koelle et al., 1991) pupal stage \| >=12 hr neuron \| subset (Robinow et al., 1993) prepupal stage salivary gland imaginal ring (Talbot et al., 1993) prepupal stage histoblast nest (Talbot et al., 1993) adult stage, oogenesis stage follicle cell (Carney and Bender, 2000) pupal stage (Koelle et al., 1991) prepupal stage foregut imaginal ring (Talbot et al., 1993) adult stage, oogenesis stage nurse cell (Carney and Bender, 2000) embryonic stage (Koelle et al., 1991) adult stage ovary (Carney and Bender, 2000) prepupal stage hindgut imaginal ring (Talbot et al., 1993) prepupal stage prothoracic gland (Talbot et al., 1993) prepupal stage Malpighian tubule (Talbot et al., 1993) prepupal stage foregut imaginal ring (Talbot et al., 1993) pupal stage \| >=12 hr ventral nervous system \| restricted