A Database of Drosophila Genes & Genomes

FB2008_07, released August 8, 2008
 

Gene Dmel\ecd

General Information
SymbolDmel\ecdSpeciesD. melanogaster
NameecdysonelessAnnotation symbolCG5714
Feature typeprotein_coding_geneFlyBase IDFBgn0000543
Created / Updated2003-12-01/2003-12-01
Genomic Location
Chromosome (arm)3LRecombination map3-1.3
Cytogenetic map62D4-62D4Sequence location3L:2,263,581..2,265,811 [-]
Map ( GBrowse ) detailed view
hide Summary Information
Automatically generated summary

See sections below for more information
The gene ecdysoneless is referred to in FlyBase by the symbol ecd (CG5714, FBgn0000543). It has the cytological map location 62D4. Its sequence location is 3L:2263581..2265811. Its molecular function is unknown. It is involved in the biological processes described with 13 unique terms, many of which group under: anatomical structure development; embryonic development via the syncytial blastoderm; embryonic development ending in birth or egg hatching; pheromone metabolic process; organelle organization and biogenesis; ecdysteroid biosynthetic process; instar larval development; response to symbiont; molting cycle, chitin-based cuticle; gamete generation. 12 alleles are reported. The phenotypes of these alleles are annotated with 27 unique terms, many of which group under: anatomical structure; organ system; egg; imaginal precursor; extended germ band embryo; thoracic segment; hemocyte; follicle cell; larval thorax; embryonic/larval hemocoel; gastrula embryo; blastoderm embryo; dorsal thoracic disc; endocrine system; morphogenetic furrow; synapse; cytoplasmic part; cephalopharyngeal skeleton; peripheral nervous system. It has one annotated transcript and one annotated polypeptide.

External Summaries

hide Phenotypic Description from the Red Book (Lindsley & Zimm 1992)
Gene/Allele symbols may differ from current usage
ecd: ecdysone
Temperature-sensitive recessive lethal. At 29, the nine-fold increase in β-ecdysone content during embryogenesis occurs normally in ecd homozygotes. Accompanied by normal increases in dopa-decarboxylase and dopamine acetyltransferase activity (Marsh and Wright, 1980, Dev. Biol. 80: 379-87). The four-fold increase during the first larval instar is reduced to 40% of normal; the additional twelve-fold increase normally occurring at pupariation eliminated. Embryonic development of ecd at 29 normal, but first larval molt delayed and death usually occurs by end of second instar; shift down to 20 at mid second instar produces full yield of adult progeny. Larvae shifted from 20 to 29 midway through third instar fail to pupate and survive as larvae for up to 3 weeks; ring gland, salivary glands, and brain of nonpupariating larvae are smaller than wild type; such ring glands cultured in vitro secrete ecdysone at lower than normal levels. Effects of 29 on third instar reversible by ecdysone feeding or by shift down to 20 within 3-5 days of shift up; after that imaginal disks cannot differentiate; ecd imaginal disks develop normally at 29 when implanted in a wild-type host. A heat pulse during larval development results in cell death with consequent abnormalities in emerging adults. At 29, third instar larvae exhibit abnormally low dopadecarboxylase activity (Kraminsky, Clark, Estelle, Gietz, Sage, O'Connor, and Hodgetts, 1980, Proc. Nat. Acad. Sci. USA 77: 4175-79); Marsh and Wright) and high urate oxidase activity (Krase and Friedman, 1979, Genetics 91: s62-63); ecdysone feeding effects normal levels. Transfer to restrictive conditions at the beginning of the pupal stage leads to death as pharate adults and to the elimination of mechanosensory chaetae; Non-sensory chaetae and other sensilla not affected. Chaetae loss is autonomous as seen in homozygous clones produced by somatic exchange and in ecd discs passed through metamorphosis in wild type hosts under restrictive conditions (Sliter, 1989, Development, 106: 347-54). Sensitivity to restrictive temperature disappears in 24 hour pupae. ecd fails to block midpupal increase in ecdysone titer (Marsh and Wright). Shifting newly emerged ecd adults to 29 results in drastically reduced ecdysone titers and sterilizes both males and females; reversible by shift back to 20; temperature-sensitive periods and therefore the times that ecdysone is required for embryonic development, chorion formation, and vitellogenesis are 1-2 days before oviposition, 24 hr prior to oviposition, and prior to stage 7, respectively (Audit-Lamour and Bussin, 1981, J. Insect Physiol. 27: 829-37).
hide Detailed Mapping Data
FlyBase Computed Cytological Location
Cytogenetic map
Evidence for location
62D4-62D4  
Limits computationally determined from genome sequence between P{PZ}slsrL182 and P{PZ}Spn06911  
Experimentally Determined Cytological Location
Cytogenetic map
Notes
References
Experimentally Determined Recombination Data
Location
3-1.3
(Sliter, 1987)
Left of (cM)
(Sliter, 1987)
Right of (cM)
(Sliter, 1987)
Notes
msn maps less than 1cM from ecd.
(Sorrentino et al., 2002)
Molecular Map Data
Gene Order (in direction of increasing cytology)
References
Gene Order (overall orientation not stated)
References
hide Gene Model & Products
Please see the GBrowse view of Dmel\ecd for information on other features
detailed view FBtr0072904 FBtr0072878 FBtr0072903 FBtr0072879 FBpp0072782 FBpp0072757 FBpp0072781 FBpp0072758 FBti0049389 FBti0039321 FBti0066205 FBti0066373 FBti0066374
Comments on Gene Model
hide Transcript Data
Annotated Transcripts
Name
FlyBase ID
RefSeq ID
Length (nt)
Associated CDS (aa)
ecd-RA
FBtr0072903
 NM_139450
  2158
  684
Additional Transcript Data & Comments
Reported size (kB)
Comments
External Data
Crossreferences
hide Polypeptide Data
Annotated Polypeptides
Name
FlyBase ID
Predicted MW (kD)
Length (aa)
Theoretical pI
RefSeq ID
GenBank protein
ecd-PA  
FBpp0072781  
77.9  
684  
4.50  
  NP_647707
  AAF47628
Additional Polypeptide Data & Comments
Reported size (kD)
Comments
External Data
Linkouts
Crossreferences
InterPro domains - A database of protein families, domains, and functional sites
•
SGT1 (IPR010770)
hide Sequences Consistent with the Gene Model
DDBJ /
EMBL /
GenBank
DNA sequence
Protein sequence
Name
AA697209
 
AE003474
AAF47628
 
AI238341
 
AI293548
 
AI297789
 
AI513301
 
AI517566
 
AI519836
 
AI533333
 
AI543715
 
AY051483
AAK92907
 
BE979083
 
BF491513
 
BG632404
 
BI577420
 
BI636727
 
 
UniProtKB/Swiss-Prot
UniProtKB/TrEMBL
    Maps to
    Does NOT map to
    Identified with
    (BDGP Project Members, 2000-)
    hide Mapped Features & Mutations
    Please see GBrowse or insertion reports for information on insertions of transgenic constructs and features not listed here
    Type
    Symbol & Location
    Additional Notes
    References
    point mutation
    ecd[2]
    3L:2,265,574..2,265,574
    evidence=experimental
    na_change=C2249106T
    pr_change=Q68@|ecd-PA
    reported_na_change=C?T
    reported_pr_change=Q67@
    (Gaziova et al., 2004)
    point mutation
    ecd[l(3)23]
    3L:2,263,755..2,263,755
    evidence=experimental
    na_change=C2247287T
    pr_change=Q650@|ecd-PA
    reported_na_change=C?T
    reported_pr_change=Q650@
    (Gaziova et al., 2004)
    point mutation
    ecd[1]
    3L:2,263,737..2,263,737
    evidence=experimental
    na_change=C2247269T
    pr_change=P656S|ecd-PA
    reported_na_change=C?T
    reported_pr_change=P656S
    (Gaziova et al., 2004)
    sequence variant
    ecd[g24]
    3L:2,264,633..2,264,636
    comment=Reported as a 4-bp deletion leading to a frameshift at codon 356 changing the sequence encoding YLRSL to HGAC. The location of the deletion was not reported exactly and is approximate. The resulting amino acid sequence in the reference genome is CGAC or YGAC@, depending upon the exact location of the 4 base deletion.
    evidence=experimental
    (Gaziova et al., 2004)
    hide External Data
    Linkouts
    DEDB - Drosophila exon database: splicing graphs
    •
    Crossreferences
    hide Expression Data
    FlyBase-Curated Data
    Transcript and
    Protein data     		Please see the FlyBase Gene Expression Report for details of gene expression from the literature.
    hide Summary of Transcript Expression
    Stage
    Tissue/Position
    Reference
    Marker for
      Subcellular Localization
      CV Term
      hide Summary of Polypeptide Expression
      Stage
      Tissue/Position
      Reference
      Marker for
        Subcellular Localization
        CV Term
        hide Microarray Data
        Developmental timecourse, Costello et al., 2008 (Original data from Arbeitman et al., 2002)
        Untitled Document detailed view FBtr0072904 FBtr0072878 FBtr0072903 FBtr0072879
        AI238341
        hide External Data & Images
        Linkouts
        FLIGHT - Cell culture data for RNAi and other high-throughput technologies
        •
        hide Alleles & Phenotypes
        hide Summary of Allele Phenotypes
        Lethality
        Allele
        lethal | recessive | conditional - heat sensitive
        lethal | dominant | partially | pharate adult stage
        lethal | dominant | embryonic stage | maternal effect | partially
        lethal | conditional - heat sensitive
        lethal | embryonic stage | conditional - heat sensitive
        lethal | larval stage
        lethal | larval stage (with Df(3L)R-R2)
        lethal | larval stage (with ecd1)
        lethal | larval stage (with ecd2)
        lethal | larval stage (with ecdl(3)23)
        lethal | larval stage | recessive
        lethal | larval stage | recessive | conditional - heat sensitive
        lethal | pharate adult stage | conditional - heat sensitive
        Other Phenotypes
        Allele
        visible | recessive | conditional - heat sensitive
        short lived
        neuroanatomy defective | larval stage | recessive | conditional - heat sensitive
        immune response defective | recessive | conditional - heat sensitive
        immune response defective | conditional - heat sensitive (with Df(3L)R-G7)
        developmental rate defective
        developmental rate defective (with Df(3L)R-R2)
        developmental rate defective (with ecd2)
        developmental rate defective (with ecdl(3)23)
        Sterility
        Allele
        female sterile | recessive | temperature conditional
        female sterile | recessive | conditional - heat sensitive
        female sterile | germ-line clone
        Phenotype manifest in
        Allele
        lymph gland (with Df(3L)R-G7)
        eye | conditional - heat sensitive
        morphogenetic furrow | conditional - heat sensitive
        egg chamber
        nurse cell
        follicle cell
        yolk
        egg
        blastoderm embryo
        stomodeal invagination
        amnioproctodeal invagination
        extended germ band embryo
        prothoracic gland | conditional - heat sensitive
        lamellocyte
        hemocyte
        eye-antennal disc | conditional - heat sensitive
        dorsal mesothoracic disc | conditional - heat sensitive
        ventral thoracic disc | conditional - heat sensitive
        macrochaeta & adult thorax | conditional ts
        larva
        vitelline membrane
        chorion
        Golgi stack | conditional - heat sensitive
        dorsal closure embryo
        embryonic/first instar larval cuticle
        border follicle cell
        synapse | conditional - heat sensitive
        mouth hooks (with ecd2)
        mouth hooks (with Df(3L)R-R2)
        mouth hooks (with ecdl(3)23)
        macrochaeta & adult thorax
        egg chamber | germ-line clone
        nurse cell | germ-line clone
        mouth hooks (with ecd1)
        hide Classical Alleles ( 8 )
        For All Classical Alleles Show

        Allele of ecdClassMutagenStocksKnown lesion
        ecd1amorph, hypomorph4 Yes
        ecd2hypomorph0 Yes
        ecd3D0 --
        ecdg240 Yes
        ecdg40 --
        ecdg50 --
        ecdg60 --
        ecdl(3)23
          		0 Yes
          hide Alleles Carried on Transgenic Constructs ( 4 )
          For All Alleles Carried on Transgenic Constructs Show

          Allele of ecdClassMutagenStocksKnown lesion
          ecd+tE50 Yes
          ecd+tS40 Yes
          ecdGD128052 Yes
          ecdScer\UAS.cGa0 Yes
          hide Aneuploid Aberrations
          Useful deficiency
           
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