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Gene Dmel\eag

General Information
Symbol	Dmel\eag	Species	D. melanogaster
Name	ether a go-go	Annotation symbol	CG10952
Feature type	protein_coding_gene	FlyBase ID	FBgn0000535
Created / Updated	2003-12-01/2003-12-01
Genomic Location
Chromosome (arm)	X	Recombination map	1-50.0
Cytogenetic map	13A2-13A5	Sequence location	X:14,865,289..14,899,359 [+]
Map ( GBrowse )	Decorated FastA GenBankGFF Gene region Extended Gene region CDSIntronsExonsTranslationsTranscripts3' UTR5' UTR
Summary Information
Automatically generated summary See sections below for more information	The gene ether a go-go is referred to in FlyBase by the symbol eag (CG10952, FBgn0000535). It has the cytological map location 13A2-13A5. Its sequence location is X:14865289..14899359. Its molecular function is described as: voltage-gated potassium channel activity; two-component sensor activity. It is involved in the biological processes: flight behavior; potassium ion transport; courtship behavior; behavioral response to ether; learning and/or memory; regulation of heart contraction; sensory perception of smell; perineurial glial growth; regulation of transcription, DNA-dependent; two-component signal transduction system (phosphorelay). 20 alleles are reported. The phenotypes of these alleles are annotated with: adult brain; neuromuscular junction; synapse; leg. It has 2 annotated transcripts and 2 annotated polypeptides.
Phenotypic Description from the Red Book (Lindsley & Zimm 1992)
Gene/Allele symbols may differ from current usage	eag: ether-a-go-go (J.C. Hall) Abnormal leg shaking under ether anaesthesia; aberrant, repetitive firing of action potentials in larval nerves; potassium currents abnormal in larval muscles (Wu, Ganetzky, Haugland and Lin, 1983, Science 220: 1076-1078). eag Sh double mutants display greatly increased level of spontaneous neuronal activity and extreme behavioral phenotypes (Burg and Wu, 1989, Dev. Biol. 131: 505-14).
Detailed Mapping Data
FlyBase Computed Cytological Location
Cytogenetic map Evidence for location 13A2-13A5   Limits computationally determined from genome sequence between P{EP}EP334&P{EP}EP977 and P{EP}hiwEP1305
Experimentally Determined Cytological Location
Cytogenetic map Notes References 13A2-13A5 (determined by in situ hybridisation)   (Drysdale et al., 1991)
Experimentally Determined Recombination Data
Location	1-50.0   1-45.3 (Homyk and Grigliatti, 1983)
Left of (cM)	sd (Cardnell et al., 2006)
Right of (cM)	g (Cardnell et al., 2006)
Notes
Molecular Map Data
Gene Order (in direction of increasing cytology) References In direction of increasing cytology: anon-13Aa? eag? (Drysdale et al., 1991) Gene Order (overall orientation not stated) References
Gene Model & Products
Please see the GBrowse view of Dmel\eag for information on other features
Comments on Gene Model
Transcript Data
Annotated Transcripts
Name FlyBase ID RefSeq ID Length (nt) Associated CDS (aa) eag-RA FBtr0073955  NM_078603   4057   1174 eag-RB FBtr0111009  NM_001042810   4345   1270
Additional Transcript Data & Comments
Reported size (kB)	10, 5.5, 4.5, 2.4 (northern blot) (Drysdale et al., 1991)
Comments
External Data
Crossreferences
Polypeptide Data
Annotated Polypeptides
Name FlyBase ID Predicted MW (kD) Length (aa) Theoretical pI RefSeq ID GenBank protein eag-PA   FBpp0073772   126.4   1174   7.60     NP_511158   AAF48410 eag-PB   FBpp0110308   136.0   1270   8.19     NP_001036275   ABI30980
Additional Polypeptide Data & Comments
Reported size (kD)	1174 (aa) (Warmke et al., 1991)
Comments
External Data
Linkouts	PANTHER - Protein classification by function, families, and pathways • FBgn0000535
Crossreferences	InterPro domains - A database of protein families, domains, and functional sites • PAS (IPR000014) Cyclic nucleotide-binding (IPR000595) PAS-associated, C-terminal (IPR000700) Potassium channel, voltage-dependent, EAG/ELK/ERG (IPR003938) Potassium channel, voltage-dependent, EAG (IPR003949) Ion transport (IPR005821) PAC motif (IPR001610) RmlC-like jelly roll fold (IPR014710)
Sequences Consistent with the Gene Model
DDBJ / EMBL / GenBank	DNA sequence Protein sequence Name AE003497 AAF48410   BT025897   BZ665518   CO336936   CZ489068   CZ489069   EC058354   EC198967   EC265140   EL871048   EL871289   EL871290   EL871413   EL871414   M61157 AAA28495
UniProtKB/Swiss-Prot	Q02280
UniProtKB/TrEMBL
Maps to
Does NOT map to
Identified with
Mapped Features & Mutations
Please see GBrowse or insertion reports for information on insertions of transgenic constructs and features not listed here Type Symbol & Location Additional Notes References point mutation eag[VV]-1 X:14,887,792..14,887,792 comment=Site of nucleotide substitution in mutant inferred by FlyBase based on reported amino acid change. Position of mutation on reference sequence inferred by FlyBase curator based on author statement. One of two amino acid substitutions in mutant. evidence=experimental na_change=C14887792T pr_change=A251V|eag-PB,A251V|eag-PA reported_pr_change=A249V (Cardnell et al., 2006) point mutation eag[G297E] X:14,890,012..14,890,012 comment=Site of nucleic acid difference inferred by FlyBase based on reported amino acid change. evidence=experimental na_change=G14890012A pr_change=G297E|eag-PB,G297E|eag-PA reported_pr_change=G297E (Cardnell et al., 2006, Cardnell et al., 2006) point mutation eag[VV]-2 X:14,894,845..14,894,845 comment=Site of nucleotide substitution in mutant inferred by FlyBase based on reported amino acid change. One of two amino acid substitutions in mutant. evidence=experimental na_change=A14894845T pr_change=E762V|eag-PB,E762V|eag-PA reported_pr_change=E762V (Cardnell et al., 2006)
External Data
Linkouts	DEDB - Drosophila exon database: splicing graphs • 2840
Crossreferences
Expression Data
FlyBase-Curated Data
Transcript and Protein data	Please see the FlyBase Gene Expression Report for details of gene expression from the literature.
Summary of Transcript Expression
Stage Tissue/Position Reference  adult stage  adult head (Drysdale et al., 1991)
Marker for
Subcellular Localization
CV Term
Summary of Polypeptide Expression
Stage Tissue/Position Reference
Marker for
Subcellular Localization
CV Term
External Data & Images
Linkouts	FLIGHT - Cell culture data for RNAi and other high-throughput technologies • FBgn0000535 FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array • FBgn0000535 GEO (NCBI) - Gene expression data: microarray and other high-throughput technologies • FBgn0000535
Alleles & Phenotypes
Summary of Allele Phenotypes
Lethality Allele viable eag1 Other Phenotypes Allele behavior defective eag1 eag24 eag4PM behavior defective | recessive eag1 eagX6 eaghd14 eaghd15 eagsc29 behavior defective | conditional - heat sensitive eag101 hyperactive eag102 hyperactive | recessive eagX6 eaghd14 eaghd15 eagsc29 hyperactive | conditional - heat sensitive, with Scer\GAL4hs.PB eagΔ932.Scer\UAS hyperactive, with Scer\GAL4D42 eagΔ932.Scer\UAS learning defective eag1 eag4PM memory defective eag4PM courtship defective, with Scer\GAL4MJ63 eagΔ932.Scer\UAS courtship defective, with Scer\GAL4MJ286 eagΔ932.Scer\UAS courtship defective, with Scer\GAL4MJ146 eagΔ932.Scer\UAS song defective, with Scer\GAL47B eagΔ932.Scer\UAS song defective, with Scer\GAL453B eagΔ932.Scer\UAS song defective, with Scer\GAL4c309 eagΔ932.Scer\UAS song defective, with Scer\GAL4OK348 eagΔ932.Scer\UAS song defective, with Scer\GAL440B eagΔ932.Scer\UAS song defective, with Scer\GAL428A eagΔ932.Scer\UAS song defective, with Scer\GAL4c747 eagΔ932.Scer\UAS song defective, with Scer\GAL46J3 eagΔ932.Scer\UAS song defective, with Scer\GAL4MJ63 eagΔ932.Scer\UAS song defective, with Scer\GAL4MJ146 eagΔ932.Scer\UAS song defective, with Scer\GAL429B eagΔ932.Scer\UAS olfaction defective eag4PM olfaction defective | recessive eag1 eagX6 eaghd15 eagsc29 taste defective eag4PM neurophysiology defective eag1 eag4PM neurophysiology defective | recessive eag1 neurophysiology defective | conditional - heat sensitive, with Scer\GAL4hs.PB eagΔ932.Scer\UAS neurophysiology defective, with Scer\GAL4D42 eagΔ932.Scer\UAS chemical sensitive eag1 chemical sensitive | recessive eag1 eaghd14 eaghd15 eagsc29 Phenotype manifest in Allele adult brain eag1 neuromuscular junction eag1 synapse eag1 leg eag1
Classical Alleles ( 18 )
For All Classical Alleles Show
Allele of eag Class Mutagen Stocks Known lesion eag101 ethyl methanesulfonate 0 -- eag102 ethyl methanesulfonate 0 -- eag1 hypomorph ethyl methanesulfonate 2 Yes eag24 0 -- eag4PM ethyl methanesulfonate 0 -- eagEY00714 P-element activity 1 -- eagG297E gain of function ethyl methanesulfonate 0 Yes eagMB03032 1 -- eagVV 0 Yes eagX6 amorph γ ray 0 -- eagf06369 piggyBac transposase 1 -- eaghd14 PM hybrid dysgenesis 0 Yes eaghd15 PM hybrid dysgenesis 0 Yes eaghd15r1 PM hybrid dysgenesis 0 Yes eaghd15r2 PM hybrid dysgenesis 0 Yes eaghd15r3 PM hybrid dysgenesis 0 Yes eagsc29 amorph, loss of function 2 -- eagunspecified 0 --
Alleles Carried on Transgenic Constructs ( 2 )
For All Alleles Carried on Transgenic Constructs Show
Allele of eag Class Mutagen Stocks Known lesion eagGD3363 in vitro construct | RNAi in vitro construct | regulatory fusion 2 Yes eagΔ932.Scer\UAS in vitro construct | deletion in vitro construct | regulatory fusion 1 Yes
Aneuploid Aberrations
Useful deficiency	Df(1)RK3
Disrupted in	Df(1)RK2 (Drysdale et al., 1991) Df(1)RK3 (Drysdale et al., 1991) Df(1)RK4 (Drysdale et al., 1991) Df(1)RK5 (Drysdale et al., 1991) In(1)sc29 (Hardie, 1991, Leibovitch et al., 1995) Tp(1;2)eagX6 (Drysdale et al., 1991)
Not disrupted in	Df(1)KA9 (Drysdale et al., 1991)
Not duplicated in	Dp(1;f)LJ9 (Drysdale et al., 1991)
Transgenic Constructs & Insertions
Transgenic Constructs
	Type of construct Name Expression data UAS construct P{GD3363} NA P{UAS-eag.Δ932} NA
Insertions
	Type of insertions Name Expression data miscellaneous insertions H{}eaghd14 NA PBac{WH}eagf06369 NA P{}eaghd15 NA insertion of enhancer trap binary system Mi{ET1}eagMB03032 No insertion of mobile activating element P{EPgy2}eagEY00714 NA
Related Comments
	Please look at the allele reports for the complete phenotype data Sequence and voltage clamp analysis demonstrate that the eag locus encodes a subunit common to different K+ channels. Combinatorial assembly of polypeptides from different genes may contribute to potassium channel diversity. (Zhong and Wu, 1991) The eag polypeptide is more closely related to polypeptides of cyclic nucleotide gated cation channels than to those of voltage gated K+ channels. (Guy et al., 1991) Effects of potassium channel blocking drugs on the presynaptic action potential repolarization after electrotonic stimulation was studied. At least four K+ currents contribute to repolarization of the nerve terminal. (Gho and Ganetzky, 1992) In eag,Sh hyperexcitable double mutants nerve/muscle synaptic ultrastructure is dramatically altered. Two types of synaptic vesicle are depleted and a third is altered in appearance, and there are changes in number and appearance of synaptic densities, and multivesicular bodies. (Jia et al., 1993) Ion channel mutants alter synaptic activity at the embryonic neuromuscular junction (NMJ). GluRIIA expression in the postsynaptic membrane is reduced by changes in presynaptic electrical activity. The size of the synaptic domain depends on the level of neural activity during embryonic synaptogenesis. (Broadie and Bate, 1993) The eag locus provides a subunit common to different K+ channels. The role of the eag subunit for modulating channels, as opposed to that of Sh subunits required for gating, selectivity and conductance of the channel, suggests a combinatorial genetic framework for generating diversified K+ channels. (Zhong and Wu, 1993) eag encodes a subunit common to different K+ channels, this supports the idea that combinatorial assembly of different polypeptides contributes to the diversity of K+ channels. (Zhong and Wu, 1991) Sequence analysis of eag genomic and cDNA clones suggest that eag encodes a previously unidentified structural component of voltage sensitive potassium channels. (Warmke et al., 1991) There are parallels in the physiological and behavioural phenotypes caused by eag mutations and Rat\CamKII-I. Interactions of eag and Rat\CamKII-I have been investigated at the behavioural, physiological, genetical and biochemical level. (Griffith et al., 1994) Mutations in Khc enhance the para and mel and suppress the Sh and eag mutant phenotypes. (Hurd et al., 1996) Fas2 is necessary for the synaptic sprouting induced by increased activity (eag Sh double mutants) or increased cAMP (dnc). (Schuster et al., 1996) In hyperexitable eag Sh mutants the pattern of endogenous Appl expression is altered and the satellite bouton-promoting activity elicited by overexpression of Appl is partially attenuated. (Torroja et al., 1999) Mutants are hypersensitive to paraquat. (Wang et al., 2000)
Gene Ontology: Function, Process & Cellular Component ( 14 )
Molecular Function
CV term Evidence References
two-component sensor activity inferred from electronic annotation with InterPro:IPR000700 (FlyBase Curators et al., 2004-)
voltage-gated potassium channel activity traceable author statement (Littleton and Ganetzky, 2000) inferred from electronic annotation with InterPro:IPR003938, InterPro:IPR003949 (FlyBase Curators et al., 2004-)
Biological Process
CV term Evidence References
behavioral response to ether non-traceable author statement (Sokolowski, 2001)
courtship behavior traceable author statement (Greenspan and Ferveur, 2000) non-traceable author statement (Sokolowski, 2001)
flight behavior inferred from mutant phenotype (Homyk and Sheppard, 1977)
learning and/or memory non-traceable author statement (Sokolowski, 2001) inferred from direct assay (Honjo and Furukubo-Tokunaga, 2005)
perineurial glial growth inferred from genetic interaction with FLYBASE:poe; FB:FBgn0011230 (Yager et al., 2001)
potassium ion transport traceable author statement (Littleton and Ganetzky, 2000) inferred from electronic annotation with InterPro:IPR003938, InterPro:IPR003949 (FlyBase Curators et al., 2004-)
regulation of heart contraction non-traceable author statement (Sokolowski, 2001)
regulation of transcription, DNA-dependent inferred from electronic annotation with InterPro:IPR001610 (FlyBase Curators et al., 2004-)
sensory perception of smell non-traceable author statement (Sokolowski, 2001)
two-component signal transduction system (phosphorelay) inferred from electronic annotation with InterPro:IPR000700 (FlyBase Curators et al., 2004-)
Cellular Component
CV term Evidence References
integral to plasma membrane non-traceable author statement (Swiss-Prot Project Members, 1993.7.1)
voltage-gated potassium channel complex inferred from sequence or structural similarity with EMBL:Z34264; protein_id:CAA84018 (FlyBase, 1992-) traceable author statement (Littleton and Ganetzky, 2000)
Sequence Ontology: Class of Gene
	protein_coding_gene   nuclear_gene
Interactions & Pathways
Summary of Genetic Interactions
Interacts with	Please look at the allele data for full details of the genetic interactions eag allele Gene References eag1 poe (Yager et al., 2001) Khc (Hurd et al., 1996) Sh (Zhong and Wu, 1993, Torroja et al., 1999, Wang et al., 2000) Hk (Wang et al., 2000) Nf1 (Yager et al., 2001) qvr (Wang et al., 2000) eag24 Sh (Zhong and Wu, 1993) eag4PM Sh (Zhong and Wu, 1993) qvr (Wang et al., 2000) unspecified dnc (Zhong et al., 1992) Sh (Cardnell et al., 2006, Engel and Wu, 1992, Jia et al., 1993, Schuster et al., 1996, Fergestad et al., 2006) mle (Budnik et al., 1990, Wang et al., 2000)
External Data
Linkouts	BioGRID - Interaction data, including yeast 2-hybrid and genetic interactions • 58786
Orthologs
Genome-wide drosophilid orthologs	Dana\GF22073 Dere\GG17855 Dmoj\GI15206 Dper\GL26721 Dpse\GA23028 Dsec\GM12050 Dvir\GJ16616 Dwil\GK20120 Dyak\GE17158
Curated drosophilid orthologs	Dpse\GA10663
Linkouts	InParanoid orthologs - Eukaryotic orthologs • ENSANGP00000002689 Anopheles gambiae (Ensembl) ENSBTAP00000011477 Bos taurus (Ensembl) ENSCAFP00000023130 Canis familiaris (Ensembl) ENSCINP00000001460 Ciona intestinalis (Ensembl) NEWSINFRUP00000172890 Takifugu rubripes (Ensembl) ENSGALP00000016040 Gallus gallus (Ensembl) ENSP00000321427 Homo sapiens (Ensembl) ENSMMUP00000021184 Macaca mulatta (Ensembl) ENSMODP00000011135 Monodelphis domestica (Ensembl) ENSPTRP00000010892 Pan troglodytes (Ensembl) ENSRNOP00000013275 Rattus norvegicus (Ensembl) GSTENP00014307001 Tetraodon nigroviridis (Ensembl) ENSXETP00000031579 Xenopus tropicalis (Ensembl) Q5JM04 Oryza sativa (Gramene) Q6K3T2 Oryza sativa (Gramene) AT4G22200.1 Arabidopsis thaliana (Arabidopsis) WBGene00028880 Caenorhabditis briggsae (Wormbase) WBGene00001171 Caenorhabditis elegans (Wormbase) MGI:3584508 Mus musculus (Informatics)
Functional Complementation between Species
Inter-Species Misexpression Data
Produces phenotype in
Produces NO phenotype in
Stocks & Reagents
Stocks Listed in FlyBase ( 10 )
Kyoto	107098 eag1 106314 In(1)sc29, sc29 wa eagsc29
Bloomington	3561 eag1 8187 wz; P{UAS-eag.Delta932}2/CyO 24184 w1118 Mi{ET1}eagMB03032 15038 y1 w67c23 P{EPgy2}eagEY00714 1442 In(1)sc29, sc29 wa eagsc29
Harvard	- PBac{WH}eagf06369
VDRC	v9127 w1118; P{GD3363}v9127 v9126 w1118; P{GD3363}v9126/TM3
Genomic Clones ( 1 )
	BACR10J19
cDNA Clones ( 5 )
	Please Note This section lists cDNAs and ESTs that fall within the genomic extent of the gene model, which may include cDNAs and ESTs of genes within introns, or of overlapping genes. Please see GBrowse for alignment of the cDNAs and ESTs to the gene model.
cDNA Clones, Fully Sequenced
BDGP DGC clones	IP03313
Other clones
cDNA Clones, End Sequenced (ESTs)
BDGP DGC clones
Other clones	EN13520 IP03513 IP03213 IP03413
RNAi & Array Information
Affy Oligo
Linkouts	DRSC - RNAi screening (Harvard): high-throughput cell culture data and design • FBgn0000535 GenomeRNAi - RNAi phenotypes (Heidelberg): high-throughput cell culture data • FBgn0000535
Antibody Information
Other Information
Discoverer
Etymology
Identification
Position Effect Variegation Data
Relationship to Other Genes
Source for database identity of	Source for identity of: eag CG10952 (Wang, 1999.11)
Source for database merge of
Additional comments
Comments About Role
	The product of the eag locus is involved in the function of one or more type of voltage-gated potassium channel. (Drysdale et al., 1991) eag expression in Xenopus oocytes generates channels that have properties of both voltage- and ligand-gated channels. The ability to mediate potassium outward and calcium inward currents is dependent on voltage and cAMP, these properties are likely to be important in the modulation of synaptic efficiency in both the central and peripheral nervous system. (Brueggemann et al., 1993) eag channels are voltage-dependent, outwardly rectifying and highly selective for K+ over Na+ ions. In contrast to results published in FBrf0064383, the eag channel electrophysiologi