HomeGene Dmel\dsh
| General Information | ||||
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| Symbol | Dmel\dsh | Species | D. melanogaster | |
| Name | dishevelled | Annotation symbol | CG18361 | |
| Feature type | protein_coding_gene | FlyBase ID | FBgn0000499 | |
| Created / Updated | 2003-12-01/2003-12-01 | |||
| Genomic Location | ||||
| Chromosome (arm) | X | Recombination map | 1-34.5 | |
| Cytogenetic map | 10B4-10B5 | Sequence location | X:11,249,914..11,252,759 [+] | |
| Map ( GBrowse ) | 
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Summary Information
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Automatically generated summary
See sections below for more information | The gene dishevelled is referred to in FlyBase by the symbol dsh (CG18361, FBgn0000499). It has the cytological map location 10B4-10B5. Its sequence location is X:11249914..11252759. Its molecular function is described as: Notch binding; protein binding; signal transducer activity. It is involved in the biological processes described with 29 unique terms, many of which group under: anatomical structure development; sensory organ development; establishment of planar polarity; organ morphogenesis; cell projection organization and biogenesis; cell communication; gamete generation; regulation of developmental process; signal transduction; imaginal disc-derived wing hair organization and biogenesis; sexual reproduction; regulation of cellular component organization and biogenesis. 102 alleles are reported. The phenotypes of these alleles are annotated with 86 unique terms, many of which group under: adult segment; peripheral nervous system; organ system; nervous system; adult mesothoracic segment; abdominal ventral denticle belt; larval abdominal segment; embryonic/larval tracheal system; imaginal precursor; portion of tissue. It has 2 annotated transcripts and 2 annotated polypeptides. | |||
| External Summaries | ||||
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Phenotypic Description from the Red Book (Lindsley & Zimm 1992)
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| Gene/Allele symbols may differ from current usage | dsh: dishevelled
Most alleles lethal, but dsh1, although poorly
viable as homozygous females or hemizygous males,
appears to be fully viable when heterozygous to a
deficiency for the region, suggesting that the reduced viability is unrelated to dsh1. dsh1 flies have deranged
thoracic hairs, divergent and blistered wings, and ellipsoid eyes. Leg bristles, hairs, and bracts display high frequencies of abnormal polarity; extra joints or joint primordia found frequently in the first and second tarsal
joints of the first and second pairs of legs; of 270 ectopic
joints, 268 displayed inverted polarity [Held, Duarte, and
Derakhshanian, 1986, Wilhelm Roux's Arch. Dev. Biol.
195: 145-57 (fig.)]. Males and females fertile, males
weakly so. Homozygotes and hemizygotes for the lethal alleles
die as second- to early-third-instar larvae when derived from
heterozygous mothers; when derived from homozygous germ-line
clones, on the other hand, embryos with segment-polarity
defects result; only ventral cuticle is present, covered with
a lawn of setae; lack dorsal cuticle, posterior spiracles and
filzkorper material. At six to seven hours maxillary and
labial segments appear to be missing and parasegmental boundaries do not form; cell death apparent in vicinity of tracheal pits, which subsequently fuse; segmental boundaries fail
to form; organization of central nervous system seems normal.
Loss of cells of posterior segment compartments leads to discontinued production of en+ product. Viability of dsh1/dsh3,
for example, when derived from homozygous dsh3 oogenic clones,
is normal, indicating that dsh1 is wild type for the early
function.
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Detailed Mapping Data
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| FlyBase Computed Cytological Location | ||||
Cytogenetic map Evidence for location 10B4-10B5
Limits computationally determined from genome sequence between P{EP}CG11756EP1610 and P{EP}CG32666EP1452
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| Experimentally Determined Cytological Location | ||||
Cytogenetic map Notes References 10B3-10B10 (determined by in situ hybridisation)
10B3-10B10 (determined by in situ hybridisation)
10B5-10B11 (determined by in situ hybridisation)
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| Experimentally Determined Recombination Data | ||||
| Location | 1-34.5 1-33.5 | |||
| Left of (cM) | ||||
| Right of (cM) | ||||
| Notes | ||||
| Molecular Map Data | ||||
Gene Order (in direction of increasing cytology)
References In direction of increasing cytology: dsh+ anon-10Ba- hop- Gene Order (overall orientation not stated) References | ||||
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Gene Model & Products
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Please see the
GBrowse view of
Dmel\dsh
for information on other features
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| Comments on Gene Model | ||||
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Transcript Data
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| Annotated Transcripts | ||||
Name FlyBase ID RefSeq ID Length (nt) Associated CDS (aa) | ||||
| Additional Transcript Data & Comments | ||||
| Reported size (kB) | 2.7 (northern blot) 2.9, 2.6 (northern blot) | |||
| Comments | ||||
| External Data | ||||
| Crossreferences | ||||
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Polypeptide Data
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| Annotated Polypeptides | ||||
Name FlyBase ID
Predicted MW (kD)
Length (aa)
Theoretical pI
RefSeq ID
GenBank protein
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| Additional Polypeptide Data & Comments | ||||
| Reported size (kD) | 623 (aa); 70 (kD observed) 623 (aa); 70 (kD predicted) | |||
| Comments | ||||
| External Data | ||||
| Linkouts | PANTHER
- Protein classification by function, families, and pathways
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| Crossreferences | InterPro
domains - A database of protein families, domains, and functional sites
• Pleckstrin/ G-protein, interacting region (IPR000591)
DIX (IPR001158)
PDZ/DHR/GLGF (IPR001478)
Dishevelled protein (IPR003351)
Dishevelled region (IPR008339)
Winged helix repressor DNA-binding (IPR011991)
Dishevelled related protein (IPR015506)
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Sequences Consistent with the Gene Model
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| DDBJ
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EMBL / GenBank | DNA sequence Protein sequence Name | |||
| UniProtKB/Swiss-Prot | ||||
| UniProtKB/TrEMBL | ||||
| Maps to | ||||
| Does NOT map to | ||||
| Identified with | ||||
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Mapped Features & Mutations
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Please see
GBrowse
or insertion reports for information on insertions of transgenic
constructs and features not listed here
Type Symbol & Location Additional Notes References deletion dsh[3] X:11,250,670..11,251,212 comment=A deletion of 543 bases and insertion of one base that causes a frameshift after amino acid residue 94. The resulting sequence at the junction is ggtaacaatcTacggcgg. evidence=experimental point mutation dsh[A3] X:11,251,624..11,251,624 comment=Site of nucleotide substitution in mutant inferred by FlyBase based on reported amino acid change. evidence=experimental na_change=G11251624A pr_change=R413H|dsh-PA,R391H|dsh-PB reported_pr_change=R413H point mutation dsh[1] X:11,251,636..11,251,636 comment=Site of nucleotide substitution in mutant inferred by FlyBase based on reported amino acid change. evidence=experimental na_change=A11251636T pr_change=K417M|dsh-PA,K395M|dsh-PB reported_pr_change=K417M point mutation dsh[A21] X:11,251,800..11,251,800 comment=Site of nucleotide substitution in mutant inferred by FlyBase based on reported amino acid change. evidence=experimental na_change=T11251800C pr_change=C472R|dsh-PA,C450R|dsh-PB reported_pr_change=C472R rescue fragment dsh[+t7EX] X:11,247,573..11,254,076 comment=Position of rescue fragment on reference sequence inferred by FlyBase curator. evidence=experimental linked_to=SalI-XbaI_rfrag rescue fragment dsh[+t7.5] X:11,247,573..11,255,261 comment=Position of rescue fragment on reference sequence inferred by FlyBase curator. evidence=experimental linked_to=SalI-SalI_rfrag | ||||
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External Data
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| Linkouts | ||||
| Crossreferences | ||||
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Expression Data
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| FlyBase-Curated Data | ||||
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Transcript and Protein data | Please see the FlyBase Gene Expression Report for details of gene expression from the literature. | |||
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Summary of Transcript Expression
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Stage Tissue/Position
Reference
embryonic stage-adult stage
oogenesis stage,adult stage
egg chamber
larval stage
imaginal disc
embryonic stage | stage 11
embryonic stage | early
embryonic stage | ||||
| Marker for | ||||
| Subcellular Localization | ||||
| CV Term | ||||
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Summary of Polypeptide Expression
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Stage Tissue/Position
Reference
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| Marker for | ||||
| Subcellular Localization | ||||
| CV Term | ||||
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External Data & Images
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| Linkouts | FLIGHT
- Cell culture data for RNAi and other high-throughput technologies
FlyAtlas
- Adult expression by tissue, using Affymetrix Dros2 array
GEO (NCBI)
- Gene expression data: microarray and other high-throughput technologies
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Alleles & Phenotypes
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Summary of Allele Phenotypes
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Lethality Allele viable, with Scer\GAL4da.G32 Other Phenotypes Allele visible, with Scer\GAL469B visible, with Scer\GAL4GMR.PF tissue polarity (with dsh1) tissue polarity (with dsh3) tissue polarity (with dshA3) tissue polarity, with Scer\GAL469B
Sterility Allele Phenotype manifest in Allele eye, with Scer\GAL4GMR.PF leg & joint mesothoracic tergum & trichome adult thorax & microchaeta egg chamber (with dsh4) ovarian sheath (with dsh4) wing, with Scer\GAL469B
wing vein, with Scer\GAL469B wing & macrochaeta | ||||