Gene Dmel\cos

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General Information

Symbol

Dmel\cos

Species

D. melanogaster

Name

costa

Annotation symbol

CG1708

Feature type

protein_coding_gene

FlyBase ID

FBgn0000352

Created / Updated

2003-12-02/2003-12-02

Genomic Location

Chromosome (arm)

Recombination map

2-57

Cytogenetic map

43B1-43B2

Sequence location

2R:3,269,669..3,274,617 [-]

Map ( GBrowse )

Summary Information

Automatically generated summary

See sections below for more information

The gene costa is referred to in FlyBase by the symbol cos (CG1708, FBgn0000352). It has the cytological map location 43B1-43B2. Its sequence location is 2R:3269669..3274617. Its molecular function is described as: microtubule binding; motor activity; microtubule motor activity; smoothened binding; protein binding; protein kinase binding; ATP binding. It is involved in the biological processes: ovarian follicle cell development; positive regulation of hh target transcription factor activity; negative regulation of transcription factor import into nucleus; microtubule-based movement; regulation of proteolysis; smoothened signaling pathway; negative regulation of smoothened signaling pathway; positive regulation of smoothened signaling pathway; imaginal disc-derived wing morphogenesis. 83 alleles are reported. The phenotypes of these alleles are annotated with 30 unique terms, many of which group under: adult segment; organ system; adult mesothoracic segment; adult; thoracic segment; follicle cell; embryonic segment; abdominal segment 10; integumentary system; embryonic tagma; imaginal precursor; lateral plate. It has one annotated transcript and one annotated polypeptide.

External Summaries

Phenotypic Description from the Red Book (Lindsley & Zimm 1992)

Gene/Allele symbols may differ from current usage

cos: costa (P. Simpson)

Homozygotes, hemizygotes, and heteroallelic heterozygotes for class I alleles die as embryos with a normal cuticular phenotype. When derived from homozygous ovarian clones, however, abnormal deletion-duplication patterns in each segment are observed; posterior rows of abdominal denticles are replaced by mirror-image duplications of the anterior rows, including the segmental boundary, which is thereby duplicated; thoracic denticle belts missing; cos1 especially severely affected in this regard, including loss of entire segments. Heterozygotes derived from homozygous ovarian clones show slightly abnormal segmental patterning. Flies heterozygous for class I alleles or cos deficiencies and at the same time heterozygous for Cos alleles display pattern duplications of wings and halteres. Class II alleles are hemizygous lethal but homozygous viable. They display wing and haltere duplications indistinguishable from those found for cos1/Cos1 heterozygotes as described by Whittle. Flies heterozygous for class III alleles and Cos show moderately reduced viability and occasional wing duplications; flies homozygous for class III alleles show reduced viability and have wild-type phenotype, but enhance the pattern duplications associated with Cos/+; hemizygotes for class III alleles or heterozygotes with class I alleles are lethal or nearly so in the presence of Cos, the survivors invariably exhibiting pattern duplications of wings and halteres.

Detailed Mapping Data

FlyBase Computed Cytological Location

Cytogenetic map

Evidence for location

43B1-43B2

Limits computationally determined from genome sequence between P{lacW}vimar^k16722 and P{lacW}cos^k16101

Experimentally Determined Cytological Location

Cytogenetic map

Notes

References

43B1-43B2

(determined by in situ hybridisation)

(Spradling et al., 1999)

42E-43E

Placed in 42E--43E by deficiency mapping (details unspecified).

(Liu and Montell, 1999)

43B2-43C2

(Sisson et al., 1997)

43A1-43C2

(determined by in situ hybridisation)

(Scott, 1992)

43B1-43B2

(BDGP Project Members, 1994-1999)

43B-43B

(determined by in situ hybridisation)

(Torok et al., 1993)

Experimentally Determined Recombination Data

Location

2-57

(Grau and Simpson, 1987)

Left of (cM)

(Grau and Simpson, 1987)

Right of (cM)

(Grau and Simpson, 1987)

Notes

Molecular Map Data

Gene Order (in direction of increasing cytology)

References

In direction of increasing cytology: cos- anon-43Ba+

(Sisson et al., 1997)

Gene Order (overall orientation not stated)

References

Gene Model & Products

Please see the GBrowse view of Dmel\cos for information on other features

Comments on Gene Model

Transcript Data

Annotated Transcripts

Name

FlyBase ID

RefSeq ID

Length (nt)

Associated CDS (aa)

cos-RA

FBtr0089015

NM_057744

4772

1201

Additional Transcript Data & Comments

Reported size (kB)

4.9 (northern blot)

(Sisson et al., 1997)

Comments

External Data

Crossreferences

Polypeptide Data

Annotated Polypeptides

Name

FlyBase ID

Predicted MW (kD)

Length (aa)

Theoretical pI

RefSeq ID

GenBank protein

cos-PA

FBpp0088087

133.1

1201

5.64

NP_477092

AAF59270

Additional Polypeptide Data & Comments

Reported size (kD)

1201 (aa); 175 (kD observed); 133 (kD predicted)

(Sisson et al., 1997)

Comments

External Data

Linkouts

PANTHER - Protein classification by function, families, and pathways

•

FBgn0000352

Crossreferences

InterPro domains - A database of protein families, domains, and functional sites

•

Kinesin, motor region (IPR001752)

Sequences Consistent with the Gene Model

DDBJ /
EMBL /
GenBank

DNA sequence

Protein sequence

Name

O16844

Maps to

DS07185

(FlyBase, 1992-)

Does NOT map to

Identified with

(Spradling et al., 1999)

(BDGP Project Members, 2000-)

Mapped Features & Mutations

Please see GBrowse or insertion reports for information on insertions of transgenic constructs and features not listed here

Type

Symbol & Location

Additional Notes

References

rescue fragment

cos[+t6.1]
2R:3,268,955..3,275,273

comment=position of restriction fragment on reference sequence inferred by FlyBase curator
evidence=experimental
linked_to=KpnI-KpnI_rfrag

(Sisson et al., 1997)

External Data

Linkouts

DEDB - Drosophila exon database: splicing graphs

•

13544

Crossreferences

Expression Data

FlyBase-Curated Data

Transcript and
Protein data

Please see the FlyBase Gene Expression Report for details of gene expression from the literature.

Summary of Transcript Expression

Stage

Tissue/Position

Reference

embryonic stage,larval stage

(Sisson et al., 1997)

Marker for

Subcellular Localization

CV Term

Summary of Polypeptide Expression

Stage

Tissue/Position

Reference

embryonic stage | stage 9-12

(Sisson et al., 1997)

embryonic stage

segment | anterior

(Sisson et al., 1997)

embryonic stage | early

(Sisson et al., 1997)

larval stage

dorsal mesothoracic disc | anterior

(Sisson et al., 1997)

Marker for

Subcellular Localization

CV Term

cytoplasm

(Sisson et al., 1997)

Microarray Data

Developmental timecourse, Costello et al., 2008 (Original data from Arbeitman et al., 2002)

Untitled Document detailed view

AI456015

External Data & Images

Linkouts

FLIGHT - Cell culture data for RNAi and other high-throughput technologies

•

FBgn0000352

FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array

•

FBgn0000352

GEO (NCBI) - Gene expression data: microarray and other high-throughput technologies

•

FBgn0000352

Alleles & Phenotypes

Summary of Allele Phenotypes

Lethality

Allele

viable

cos¹¹

semi-viable | recessive

cos⁷

lethal

cos^k16128

lethal | recessive

lethal | partially (with cos³)

cos¹¹

lethal | partially (with cos¹¹)

cos³

lethal | embryonic stage

cos³

lethal | embryonic stage | rescuable maternal effect

lethal | embryonic stage | germ-line clone | maternal effect | recessive

cos^2R-183-20

lethal | pupal stage

cos⁷

Other Phenotypes

Allele

wild-type

cos^+t6.1

visible | recessive

cos⁷

visible | dominant

cos^k16101

visible | somatic clone

cos^unspecified

visible, with Scer\GAL4^Bx-MS1096

visible, with Scer\GAL4^ptc-559.1

cos^{CSBD.Scer\UAS.T:Ivir\HA1}

hyperplastic

cos^k16128

Phenotype manifest in

Allele

embryonic epidermis | germ-line clone

embryonic segment | germ-line clone

embryonic/first instar larval cuticle | germ-line clone

denticle belt & embryo

wing

wing vein

cos¹

follicle cell | ectopic | somatic clone

cos¹

oocyte | somatic clone

cos¹

sex comb | ectopic | somatic clone

cos¹

wing (with cos³)

cos¹¹

dorsal mesothoracic disc

cos²

denticle belt & embryo | germ-line clone

embryonic segment | germ-line clone | maternal effect

cos^2R-183-20

embryonic abdominal segment | germ-line clone | maternal effect

cos^2R-183-20

wing (with cos¹¹)

cos³

pupal epidermis

cos⁵

embryonic epidermis

cos⁵

border follicle cell | somatic clone | supernumerary

cos^52B7

border follicle cell | somatic clone

cos^52B7

border follicle cell | ectopic | somatic clone

cos^52B7

polar follicle cell | ectopic | somatic clone

border follicle cell | ectopic

cos^52B7

haltere

cos⁷

dorsal mesothoracic disc | anterior | somatic clone, with Scer\GAL4^Act5C.PP

wing | anterior, with Scer\GAL4^Bx-MS1096

wing | ectopic, with Scer\GAL4^Bx-MS1096

wing blade, with Scer\GAL4^Bx-MS1096

wing vein L3, with Scer\GAL4^Bx-MS1096

cos^{S182T.Scer\UAS.T:Avic\GFP}

wing vein L4, with Scer\GAL4^Bx-MS1096

cos^{S182T.Scer\UAS.T:Avic\GFP}

egg chamber | somatic clone, with Scer\GAL4^Act5C.PP

cos^Scer\UAS.cHa

germarium | somatic clone, with Scer\GAL4^Act5C.PP

cos^Scer\UAS.cHa

interfollicle cell | somatic clone, with Scer\GAL4^Act5C.PP

cos^Scer\UAS.cHa

female gonopod | dorsal

female gonopod | ventral

wing | posterior compartment

cos^V1

wing | anterior compartment

cos^V1

imaginal disc

wing vein L3 | ectopic | somatic clone

cos^unspecified

wing | somatic clone

cos^unspecified

wing vein L3 | proximal, with Scer\GAL4^ptc-559.1

cos^{CSBD.Scer\UAS.T:Ivir\HA1}

wing vein L4 | proximal, with Scer\GAL4^ptc-559.1

cos^{CSBD.Scer\UAS.T:Ivir\HA1}

1st posterior cell, with Scer\GAL4^ptc-559.1

cos^{CSBD.Scer\UAS.T:Ivir\HA1}

Classical Alleles ( 28 )

For All Classical Alleles Show

Allele of cos	Class	Mutagen	Stocks	Known lesion
cos¹⁰		ethyl methanesulfonate	0	--
cos¹¹	hypomorph	ethyl methanesulfonate	0	--
cos¹²		ethyl methanesulfonate	0	--
cos¹⁴	hypomorph	ethyl methanesulfonate	0	Yes
cos¹⁵		ethyl methanesulfonate	0	--
cos¹⁶		ethyl methanesulfonate	0	--
cos¹	loss of function	ethyl methanesulfonate	0	--
cos²	amorph, loss of function	X ray	0	Yes
cos^2R-183-20		ethyl methanesulfonate	0	--
cos³¹			0	--
cos³	loss of function	X ray	0	--
cos⁴	loss of function	ethyl methanesulfonate	0	--
cos^52B7		ethyl methanesulfonate	0	--
cos⁵	loss of function	ethyl methanesulfonate	3	--
cos⁶	loss of function	ethyl methanesulfonate	0	--
cos⁷	hypomorph	ethyl methanesulfonate	0	--
cos⁸		ethyl methanesulfonate	0	--
cos⁹		ethyl methanesulfonate	0	--
cos^EY08735		P-element activity	1	--
cos^V1	wild-type allele, loss of function	spontaneous	0	Yes
cos^V2	loss of function	spontaneous	0	--
cos^V3	loss of function	spontaneous	0	--
cos^V4	loss of function	spontaneous	0	--
cos^V5	loss of function	ethyl methanesulfonate	0	--
cos^k16101		P-element activity	2	Yes
cos^k16122		P-element activity	0	--
cos^k16128		P-element activity	0	--
cos^unspecified			0	--

Alleles Carried on Transgenic Constructs ( 55 )

For All Alleles Carried on Transgenic Constructs Show

Allele of cos	Mutagen	Stocks	Known lesion
cos^+t6.1	in vitro construct \| genomic fragment	0	Yes
cos^+t6.5	in vitro construct \| genomic fragment	0	Yes
cos^{1-1201.Act5C.T:Hsap\MYC}	in vitro construct \| coding region fusion in vitro construct \| regulatory fusion	0	Yes
cos^{1-605.Act5C.T:Hsap\MYC}	in vitro construct \| coding region fusion in vitro construct \| regulatory fusion	0	Yes
cos^{1-751.Act5C.T:Hsap\MYC}	in vitro construct \| coding region fusion in vitro construct \| regulatory fusion	0	Yes
cos^{1-900.Act5C.T:Hsap\MYC}	in vitro construct \| coding region fusion in vitro construct \| regulatory fusion	0	Yes
cos^{115-1201.Act5C.T:Hsap\MYC}	in vitro construct \| coding region fusion in vitro