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Gene Dmel\ck

General Information
Symbol	Dmel\ck	Species	D. melanogaster
Name	crinkled	Annotation symbol	CG7595
Feature type	protein_coding_gene	FlyBase ID	FBgn0000317
Created / Updated	2003-12-01/2003-12-01
Genomic Location
Chromosome (arm)	2L	Recombination map	2-51.0
Cytogenetic map	35B8-35B9	Sequence location	2L:15,044,961..15,057,848 [-]
Map ( GBrowse )	Decorated FastA GenBankGFF Gene region Extended Gene region CDSIntronsExonsTranslationsTranscripts3' UTR5' UTR
Summary Information
Automatically generated summary See sections below for more information	The gene crinkled is referred to in FlyBase by the symbol ck (CG7595, FBgn0000317). It has the cytological map location 35B8-35B9. Its sequence location is 2L:15044961..15057848. Its molecular function is described as: actin binding; motor activity; ATPase activity, coupled; myosin light chain binding; actin-dependent ATPase activity; ATP binding; transporter activity. It is involved in the biological processes: sensory perception of sound; antennal morphogenesis; actin filament-based movement; bristle morphogenesis; imaginal disc-derived wing hair organization and biogenesis. 34 alleles are reported. The phenotypes of these alleles are annotated with: wing hair; arista; macrochaeta; microchaeta; trichogen cell; wing; ocellar bristle. It has 2 annotated transcripts and 2 annotated polypeptides.
Phenotypic Description from the Red Book (Lindsley & Zimm 1992)
Gene/Allele symbols may differ from current usage	ck: crinkled (M. Ashburner) Recessive lethal or semilethal. ck embryos exhibit denticles thickset and forked; hairs basally fused; sensory hairs blunt [Nusslein-Volhard, Wieschaus, and Kluding, (fig.)]. Hemizygous survivors, or survivors among heteroallelic combinations that partially complement for viability, have stubbly chaetae, multiple trichomes, and feathery aristae (Gubb et al.). Variable expression of a wavy, crinkled wing phenotype. Cell viable in clones of adult epidermis; bristle and hair phenotypes autonomous; useful as a cell marker in 2L for clonal analysis (Struhl).
Detailed Mapping Data
FlyBase Computed Cytological Location
Cytogenetic map Evidence for location 35B8-35B9   Limits computationally determined from genome sequence between P{lacW}Su(H)k07904 and P{EP}vigEP812&P{PZ}stc05441
Experimentally Determined Cytological Location
Cytogenetic map Notes References 35B-35B (determined by in situ hybridisation)   (Kiehart et al., 2004) 35C1-35C3 (determined by in situ hybridisation)   (Ashburner et al., 1999) 35C1-35C2 (determined by in situ hybridisation)   (Spradling et al., 1999) 33B-33B (determined by in situ hybridisation)   (Louis et al., 1997) 35B-35B (determined by in situ hybridisation)   (EDGP Project Members, 1994-) 35C1-35C3   (BDGP Project Members, 1994-1999) 35B10-35B10   (Schweisguth and Posakony, 1992) 35B-35C (determined by in situ hybridisation)   (Chen et al., 1991)
Experimentally Determined Recombination Data
Location	2-51.0   2- (Schweisguth and Posakony, 1992)
Left of (cM)
Right of (cM)
Notes	Map position estimated.
Molecular Map Data
Gene Order (in direction of increasing cytology) References In direction of increasing cytology: l(2)35Bg- Su(H)+ ck- (Schweisguth and Posakony, 1992) In direction of increasing cytology: Su(H)+ ck- TfIIS- vas+ vig- vas+ (Tinker et al., 1998) In direction of increasing cytology: Su(H)+ ck- TfIIS- (Ashburner et al., 1999) Gene Order (overall orientation not stated) References
Gene Model & Products
Please see the GBrowse view of Dmel\ck for information on other features
Comments on Gene Model
	DGC clone LD14917 appears problematic: incomplete CDS
Transcript Data
Annotated Transcripts
Name FlyBase ID RefSeq ID Length (nt) Associated CDS (aa) ck-RA FBtr0080723  NM_165099   7229   2167 ck-RB FBtr0080724  NM_078847   7224   2167
Additional Transcript Data & Comments
Reported size (kB)
Comments
External Data
Crossreferences
Polypeptide Data
Annotated Polypeptides
Name FlyBase ID Predicted MW (kD) Length (aa) Theoretical pI RefSeq ID GenBank protein ck-PA   FBpp0080282   250.3   2167   9.24     NP_723895   AAF53435 ck-PB   FBpp0080283   250.3   2167   9.24     NP_523571   AAN10886
Additional Polypeptide Data & Comments
Reported size (kD)
Comments
External Data
Linkouts	PANTHER - Protein classification by function, families, and pathways • FBgn0000317
Crossreferences	InterPro domains - A database of protein families, domains, and functional sites • IQ calmodulin-binding region (IPR000048) Band 4.1, N-terminal (IPR000299) Unconventional myosin/plant kinesin-like protein/non-motor protein conserved region MyTH4 (IPR000857) Src homology-3 (IPR001452) Myosin head, motor region (IPR001609) Bacterial extracellular solute-binding family 1, conserved site (IPR006061) Variant SH3 (IPR011511) Cytokine, IL-1-like (IPR008996) FERM/acyl-CoA-binding protein, 3-helical bundle (IPR014352)
Sequences Consistent with the Gene Model
DDBJ / EMBL / GenBank	DNA sequence Protein sequence Name AA391840   AA440341   AA735306   AE003412 AAF44915   AE003646 AAF53435   AE003646 AAN10886   AQ025664   AQ074059   AW942331   AY069438 AAL39583   BG638078   BI213312   BI356375   BI368328   BI636656   BT011332   CO178816   CO187336   CO279187   CO279854   CO281059   CO289410   CO290983   CO300748   CO307749   CO331986   CO339118   CO339732   CO342790   CO345331   CZ486884   EC061857   EC066142   EC081672   EC081825   EC084159   EC090468   EC195070   EC197561   EC198819   EC200042   EC203032   EC216574   EC218638   EC234434   EC252344   EC252707   EC252955   EC256455   LD14874   Z70908
UniProtKB/Swiss-Prot
UniProtKB/TrEMBL	Q9V3Z6
Maps to	DS00929 (Ashburner et al., 1999.10.12)
Does NOT map to
Identified with	LD14874 (Ashburner et al., 1999.10.12) LD14917 (Ashburner et al., 1999.10.12, BDGP Project Members, 2000-, Tzolovsky et al., 2002) LD10736 (Spradling et al., 1999, Ashburner et al., 1999, Ashburner et al., 1999.10.12) SD18415 (BDGP Project Members, 2000-)
Mapped Features & Mutations
Please see GBrowse or insertion reports for information on insertions of transgenic constructs and features not listed here Type Symbol & Location Additional Notes References point mutation ck[16] 2L:15,051,956..15,051,956 comment=Site of nucleotide substitution in mutant inferred by FlyBase based on reported amino acid change. evidence=experimental na_change=G15047885A pr_change=G156E|ck-PA,G156E|ck-PB reported_pr_change=G156E (Kiehart et al., 2004) point mutation ck[14] 2L:15,050,242..15,050,242 comment=Site of nucleotide substitution in mutant inferred by FlyBase based on reported amino acid change. evidence=experimental na_change=C15046171T pr_change=P684L|ck-PA,P684L|ck-PB reported_pr_change=P684L (Kiehart et al., 2004) point mutation ck[13] 2L:15,049,991..15,049,991 comment=Site of nucleotide substitution in mutant inferred by FlyBase based on reported amino acid change. evidence=experimental na_change=C15045920T pr_change=R768|ck-PA,R768|ck-PB reported_pr_change=R768@ (Kiehart et al., 2004) point mutation ck[7] 2L:15,047,903..15,047,903 comment=Site of nucleotide substitution in mutant inferred by FlyBase based on reported amino acid change. evidence=experimental na_change=T15043832A pr_change=L1445|ck-PA,L1445|ck-PB reported_pr_change=L1445@ (Kiehart et al., 2004)
External Data
Linkouts	DEDB - Drosophila exon database: splicing graphs • 7698
Crossreferences
Expression Data
FlyBase-Curated Data
Transcript and Protein data	Please see the FlyBase Gene Expression Report for details of gene expression from the literature.
Summary of Transcript Expression
Stage Tissue/Position Reference
Marker for
Subcellular Localization
CV Term
Summary of Polypeptide Expression
Stage Tissue/Position Reference
Marker for
Subcellular Localization
CV Term
External Data & Images
Linkouts	FLIGHT - Cell culture data for RNAi and other high-throughput technologies • FBgn0000317 FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array • FBgn0000317 GEO (NCBI) - Gene expression data: microarray and other high-throughput technologies • FBgn0000317
Alleles & Phenotypes
Summary of Allele Phenotypes
Lethality Allele viable ckBG00682 ckPL00069 lethal | recessive ck07130 ck10 ck11 ck12 ck13 ck2 ck3 ck4 ck6 ck7 ck8 ck9 ckTE35BC lethal | embryonic stage | recessive ck13 lethal | larval stage | recessive ck5 ck7 semi-lethal | recessive ck07130 ck10 ck12 ck13 ck1 ck2 ck3 ck4 ck5 ck6 ck7 ck8 ck9 Other Phenotypes Allele visible ckTE35BC ckUAM21 visible (with ckUAM21) ckUAH21 visible (with ckUAH21) ckUAM21 visible | recessive ck07130 ckBG00682 auditory system defective ck13 auditory system defective (with ck13) ck07130 auditory system defective (with ck13), with Scer\GAL4elav-C155, cktail.Scer\UAS.T:Avic\GFP ck07130 auditory system defective (with ck13), with Scer\GAL4sqh.PW, cktail.Scer\UAS.T:Avic\GFP ck07130 auditory system defective (with ck07130) ck13 auditory system defective (with ck07130), with Scer\GAL4elav-C155, cktail.Scer\UAS.T:Avic\GFP ck13 auditory system defective (with ck07130), with Scer\GAL4sqh.PW, cktail.Scer\UAS.T:Avic\GFP ck13 auditory system defective | recessive ck07130 auditory system defective, with Scer\GAL4elav-C155, ck13, cktail.Scer\UAS.T:Avic\GFP ck07130 auditory system defective, with Scer\GAL4elav-C155, ck07130, ck13 cktail.Scer\UAS.T:Avic\GFP auditory system defective, with Scer\GAL4elav-C155, ck07130, cktail.Scer\UAS.T:Avic\GFP ck13 auditory system defective, with Scer\GAL4elav-C155, ck07130/ck13 cktail.Scer\UAS.T:Avic\GFP auditory system defective, with Scer\GAL4sqh.PW, ck13, cktail.Scer\UAS.T:Avic\GFP ck07130 auditory system defective, with Scer\GAL4sqh.PW, ck07130, ck13 cktail.Scer\UAS.T:Avic\GFP auditory system defective, with Scer\GAL4sqh.PW, ck07130, cktail.Scer\UAS.T:Avic\GFP ck13 auditory system defective, with Scer\GAL4sqh.PW, ck07130/ck13 cktail.Scer\UAS.T:Avic\GFP Sterility Allele sterile | recessive ck13 ck16 ck7 fertile ckPL00069 Phenotype manifest in Allele Johnston's organ & scolopidium (with ck13) ck07130 Johnston's organ & dendritic cap cell (with ck13) ck07130 wing hair | supernumerary ck07130 Johnston's organ & scolopidium ck07130 ck13 Johnston's organ & dendritic cap cell ck07130 ck13 Johnston's organ & scolopidium (with ck07130) ck13 Johnston's organ & dendritic cap cell (with ck07130) ck13 wing hair | ectopic ck13 arista ck13 ckTE35BC macrochaeta ck13 ckTE35BC microchaeta ck13 trichogen cell ck13 wing hair ck13 microchaeta & wing ckIIB33 wing ckTE35BC arista (with ckUAM21) ckUAH21 ocellar bristle ckUAM21 arista (with ckUAH21) ckUAM21
Classical Alleles ( 31 )
For All Classical Alleles Show
Allele of ck Class Mutagen Stocks Known lesion ck07130 hypomorph P-element activity 1 Yes ck10 ethyl methanesulfonate 0 -- ck11 ethyl methanesulfonate 0 -- ck12 ethyl methanesulfonate 0 -- ck13 ethyl methanesulfonate 8 Yes ck14 ethyl methanesulfonate 0 Yes ck15 ethyl methanesulfonate 0 -- ck16 ethyl methanesulfonate 0 Yes ck17 ethyl methanesulfonate 0 -- ck1 spontaneous 1 -- ck2 ethyl methanesulfonate 0 -- ck3 ethyl methanesulfonate 0 -- ck4 ethyl methanesulfonate 0 -- ck5 ethyl methanesulfonate 0 -- ck6 ethyl methanesulfonate 0 -- ck7 ethyl methanesulfonate 0 Yes ck8 ethyl methanesulfonate 0 -- ck9 ethyl methanesulfonate 0 -- ckA11 ethyl methanesulfonate 0 -- ckBG00682 P-element activity 0 Yes ckEP2051 P-element activity 1 Yes ckGJ2 ethyl methanesulfonate 0 -- ckH67 α ray 0 -- ckIIB33 0 -- ckIK3 spontaneous 0 -- ckPL00069 P-element activity 1 -- ckTE35BC 0 -- ckUAH21 0 -- ckUAM21 0 -- ckf04351 piggyBac transposase 1 -- ckunspecified 0 --
Alleles Carried on Transgenic Constructs ( 3 )
For All Alleles Carried on Transgenic Constructs Show
Allele of ck Class Mutagen Stocks Known lesion ckGD1408 in vitro construct | RNAi in vitro construct | regulatory fusion 1 Yes ckScer\UAS.T:Avic\GFP in vitro construct | regulatory fusion in vitro construct | coding region fusion 0 Yes cktail.Scer\UAS.T:Avic\GFP antimorph in vitro construct | regulatory fusion in vitro construct | coding region fusion 0 Yes
Aneuploid Aberrations
Useful deficiency	Df(2L)A220
Useful duplication	Dp(2;2)GYL
Disrupted in	Df(2L)64j (O'Donnell et al., 1977, Woodruff and Ashburner, 1979, Ashburner, 1982, Gubb et al., 1984, Ashburner et al., 1990, Furukawa et al., 1992) Df(2L)A215 (Ashburner et al., 1982, Fossett et al., 1994) Df(2L)A220 (Ashburner et al., 1982, Fossett et al., 1994) Df(2L)A246 (Ashburner et al., 1982, Fossett et al., 1994) Df(2L)A263 (Ashburner et al., 1982, Furukawa et al., 1992) Df(2L)A264 (Ashburner et al., 1982, Fossett et al., 1994) Df(2L)A267 (Cai et al., 2001, Ashburner et al., 1982, Ashburner et al., 1982, Ashburner et al., 1990, Fossett et al., 1994) Df(2L)A337 (Fossett et al., 1994) Df(2L)A376 (Ashburner et al., 1982, Ashburner, 1982, Fossett et al., 1994) Df(2L)A377 (Ashburner et al., 1982, Ashburner, 1982) Df(2L)A400 (Ashburner et al., 1982, Ashburner et al., 1982, Ashburner, 1982, Fossett et al., 1994) Df(2L)A48 (Spradling et al., 1999, Cai et al., 2001, Ashburner et al., 1982, Ashburner et al., 1982, Grau et al., 1984, BDGP Project Members, 1994-1999, Fossett et al., 1994) Df(2L)A72 (Ashburner et al., 1982, Ashburner et al., 1982, Ashburner et al., 1990, Fossett et al., 1994) Df(2L)Adh-nBR101 (Fossett et al., 1994) Df(2L)Adh-nBR102 (Fossett et al., 1994) Df(2L)Adh-nBR103 (Fossett et al., 1994) Df(2L)Adh-nBR105 (Fossett et al., 1994) Df(2L)Adh-nBR106 (Fossett et al., 1994, Roote, 1996.1.11, Roote et al., 1996, Roote, 1996.8) Df(2L)Adh-nBR107 (Fossett et al., 1994, Roote, 1996.1.11, Roote et al., 1996, Roote, 1996.8) Df(2L)Adh-nBR118 (Fossett et al., 1994) Df(2L)Adh-nBR120 (Fossett et al., 1994) Df(2L)Adh-nBR122 (Fossett et al., 1994) Df(2L)Adh-nBR125 (Fossett et al., 1994) Df(2L)Adh-nBR126 (Fossett et al., 1994, Roote et al., 1996) Df(2L)Adh-nBR128 (Fossett et al., 1994) Df(2L)Adh-nBR129 (Fossett et al., 1994) Df(2L)Adh-nBR130 (Fossett et al., 1994) Df(2L)Adh-nBR131 (Fossett et al., 1994) Df(2L)BSC254 (Christensen and Cook, 2007.3.22) Df(2L)BSC299 (Christensen and Cook, 2007.5.8) Df(2L)Exel7061 (Ryder, 2004.4.26) Df(2L)PZ07130-mr1 (Roote, 2000.6.13) Df(2L)PZ07130-mr2 (Roote, 2000.6.13) Df(2L)PZ07130-mr3 (Roote, 2000.6.13) Df(2L)PZ07130-mr5 (Roote, 2000.6.13) Df(2L)PZ07130-mr7 (Roote, 2000.6.13) Df(2L)PZ07130-mr8 (Roote, 2000.6.13) Df(2L)PZ07130-mr9 (Roote, 2000.6.13) Df(2L)Sco7 (Ashburner et al., 1990) Df(2L)Scorv23 (Ashburner, 1982, Grau et al., 1984) Df(2L)Scorv4 (Woodruff and Ashburner, 1979) Df(2L)TE35B-1 (Gubb et al., 1985) Df(2L)TE35B-2 (Gubb et al., 1985) Df(2L)TE35B-5 (Gubb et al., 1985) Df(2L)TE35B-6 (Gubb et al., 1985) Df(2L)TE35BC-1 (Ashburner et al., 1981, Ashburner, 1982, Gubb et al., 1984) Df(2L)TE35BC-23 (Gubb et al., 1984)