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Gene Dmel\bsk

General Information
Symbol	Dmel\bsk	Species	D. melanogaster
Name	basket	Annotation symbol	CG5680
Feature type	protein_coding_gene	FlyBase ID	FBgn0000229
Created / Updated	2003-12-01/2003-12-01
Genomic Location
Chromosome (arm)	2L	Recombination map	2-33
Cytogenetic map	31B1-31B1	Sequence location	2L:10,247,485..10,250,479 [-]
Map ( GBrowse )	Decorated FastA GenBankGFF Gene region Extended Gene region CDSIntronsExonsTranslationsTranscripts3' UTR5' UTR
Summary Information
Automatically generated summary See sections below for more information	The gene basket is referred to in FlyBase by the symbol bsk (CG5680, FBgn0000229). It has the cytological map location 31B1. Its sequence location is 2L:10247485..10250479. Its molecular function is described as: JUN kinase activity; protein serine/threonine kinase activity; protein binding; MAP kinase activity; ATP binding; phosphorylase kinase activity; calmodulin binding. It is involved in the biological processes described with 23 unique terms, many of which group under: anatomical structure development; ovarian follicle cell development; response to stress; biopolymer modification; cellular macromolecule metabolic process; defense response; cell motility; gamete generation; MAPKKK cascade; eggshell chorion formation; protein modification process; wound healing. 35 alleles are reported. The phenotypes of these alleles are annotated with 14 unique terms, many of which group under: anatomical structure; organ system; peripheral nervous system; integumentary system; nervous system; portion of tissue; adult; thoracic segment; dorsal thoracic disc; adult segment. It has one annotated transcript and one annotated polypeptide.
External Summaries
Phenotypic Description from the Red Book (Lindsley & Zimm 1992)
Gene/Allele symbols may differ from current usage	bsk: basket Homozygous lethal; embryos have large dorsal anterior hole.
Detailed Mapping Data
FlyBase Computed Cytological Location
Cytogenetic map Evidence for location 31B1-31B1   Limits computationally determined from genome sequence between P{lacW}me31Bk06607 and P{PZ}nmd08774
Experimentally Determined Cytological Location
Cytogenetic map Notes References 31B1-31B1   (Riesgo-Escovar, 1996.10.3) 31B-31C (determined by in situ hybridisation)   (Sluss et al., 1996) 31B-31B (determined by in situ hybridisation)   (Riesgo-Escovar et al., 1996)
Experimentally Determined Recombination Data
Location	2-33 (Tearle and Nusslein-Volhard, 1987, ) 2- (Riesgo-Escovar, 1996.10.3)
Left of (cM)
Right of (cM)
Notes
Molecular Map Data
Gene Order (in direction of increasing cytology) References In direction of increasing cytology: me31B+ chico- bsk- Ror+ (Riesgo-Escovar et al., 1996) In direction of increasing cytology: me31B+ chico- bsk- (Bohni et al., 1999) In direction of increasing cytology: Rsf1+ Pten+ CG5676+ Ror- bsk+ chico+ (Huang et al., 1999) In direction of increasing cytology: chico- bsk- Ror+ Pten- (Goberdhan et al., 1999) Gene Order (overall orientation not stated) References Overall orientation not stated: me31B+ bsk- (Sluss et al., 1996) Overall orientation not stated: chico? bsk- (Riesgo-Escovar, 1998.3.10) Overall orientation not stated: bsk+ chico+ (Kulansky Poltilove et al., 2000)
Gene Model & Products
Please see the GBrowse view of Dmel\bsk for information on other features
Comments on Gene Model
Transcript Data
Annotated Transcripts
Name FlyBase ID RefSeq ID Length (nt) Associated CDS (aa) bsk-RB FBtr0080087  NM_164900   1530   372
Additional Transcript Data & Comments
Reported size (kB)	1.5 (northern blot) (Riesgo-Escovar et al., 1996) 1.85, 1.8 (northern blot) (Sluss et al., 1996)
Comments
External Data
Crossreferences
Polypeptide Data
Annotated Polypeptides
Name FlyBase ID Predicted MW (kD) Length (aa) Theoretical pI RefSeq ID GenBank protein bsk-PB   FBpp0079676   43.0   372   6.39     NP_723541   AAF52883
Additional Polypeptide Data & Comments
Reported size (kD)	372 (aa) (Sluss et al., 1996) 353 (aa); 49 (kD observed); 49 (kD predicted) (Riesgo-Escovar et al., 1996)
Comments
External Data
Linkouts	PANTHER - Protein classification by function, families, and pathways • FBgn0000229
Crossreferences	InterPro domains - A database of protein families, domains, and functional sites • Protein kinase, core (IPR000719) Serine/threonine protein kinase (IPR002290) MAP kinase, conserved site (IPR003527) Serine/threonine protein kinase, active site (IPR008271) JNK MAP kinase (IPR008351) Protein kinase-like (IPR011009) Phosphorylase kinase, gamma catalytic subunit (IPR002291) Protein kinase ATP binding, conserved site (IPR017441) Serine/threonine protein kinase-related (IPR017442)
Sequences Consistent with the Gene Model
DDBJ / EMBL / GenBank	DNA sequence Protein sequence Name AA697511   AE003628 AAF52883   AI109623   AI259734   AY070865 AAL48487   AY122221 AAM52733   BI168174   BI169763   BI171668   BI212564   BI239448   BI243273   BI362187   BI364154   BI365741   BI374322   BI375387   BI634861   BP551355   BP551356   BT001548 AAN71303   CL705896   CO190579   CO193544   CO286658   CO303465   CO310493   CO327167   CO329989   CO344017   CO344385   CZ480636   EC051012   EC051997   EC054018   EC055010   EC063130   EC064687   EC227861   EC229537   EC229945   EC237667   EC241377   EC253851   EC259888   U49180 AAB97094   U49249 AAB48381   U50965 AAB51187   U50966 AAB51188   U73196 AAC47325   Z50635
UniProtKB/Swiss-Prot	P92208
UniProtKB/TrEMBL	Q8IGX1
Maps to
Does NOT map to
Identified with	HL02677 RE11033 RE08746 (BDGP Project Members, 2000-)
Mapped Features & Mutations
Please see GBrowse or insertion reports for information on insertions of transgenic constructs and features not listed here Type Symbol & Location Additional Notes References point mutation bsk[G368] 2L:10,248,626..10,248,626 evidence=experimental na_change=C10248626T pr_change=Q115@|bsk-PB reported_na_change=C?T reported_pr_change=Q115@ (Geuking et al., 2005) point mutation bsk[G179] 2L:10,248,589..10,248,589 evidence=experimental na_change=C10248589T pr_change=S127F|bsk-PB reported_na_change=C?T reported_pr_change=S127F (Geuking et al., 2005) point mutation bsk[G536] 2L:10,248,589..10,248,589 evidence=experimental na_change=C10248589T pr_change=S127F|bsk-PB reported_na_change=C?T reported_pr_change=S127F (Geuking et al., 2005) point mutation bsk[G577] 2L:10,248,541..10,248,541 evidence=experimental na_change=C10248541T pr_change=A143V|bsk-PB reported_na_change=C?T reported_pr_change=A143V (Geuking et al., 2005) point mutation bsk[G400] 2L:10,248,364..10,248,364 evidence=experimental na_change=C10248364T pr_change=T181I|bsk-PB reported_na_change=C?T reported_pr_change=T181I (Geuking et al., 2005) point mutation bsk[G760] 2L:10,248,362..10,248,362 evidence=experimental na_change=C10248362T pr_change=P182S|bsk-PB reported_na_change=C?T reported_pr_change=P182S (Geuking et al., 2005) point mutation bsk[G290] 2L:10,248,283..10,248,283 evidence=experimental na_change=C10248283T pr_change=S208F|bsk-PB reported_na_change=C?T reported_pr_change=S208F (Geuking et al., 2005) point mutation bsk[1] 2L:10,248,232..10,248,232 evidence=experimental na_change=G10248232A pr_change=G225E|bsk-PB reported_na_change=G?A reported_pr_change=G225E (Sluss et al., 1996, Davis, 1996.3.8) point mutation bsk[G258] 2L:10,248,210..10,248,210 comment=TGG to TGA evidence=experimental na_change=G10248210A pr_change=W232@|bsk-PB reported_na_change=G?A reported_pr_change=W232@ (Geuking et al., 2005) point mutation bsk[G688] 2L:10,247,922..10,247,922 evidence=experimental na_change=G10247922C pr_change=R308P|bsk-PB reported_na_change=G?C reported_pr_change=R308P (Geuking et al., 2005) point mutation bsk[2] 2L:10,247,899..10,247,899 evidence=experimental na_change=A10247899T pr_change=K316@|bsk-PB reported_na_change=A?T reported_pr_change=K316@ (Sluss et al., 1996) rescue fragment bsk[+t5] 2L:10,245,705..10,250,998 comment=Position of restriction fragment on reference sequence inferred by FlyBase curator. evidence=experimental linked_to=BamHI-SpeI_rfrag (Riesgo-Escovar et al., 1996)
External Data
Linkouts	DEDB - Drosophila exon database: splicing graphs • 7210
Crossreferences
Expression Data
FlyBase-Curated Data
Transcript and Protein data	Please see the FlyBase Gene Expression Report for details of gene expression from the literature.
Summary of Transcript Expression
Stage Tissue/Position Reference  adult stage | male   (Sluss et al., 1996)  embryonic stage | stage 6,7  posterior transverse furrow (Riesgo-Escovar et al., 1996)  adult stage | female   (Sluss et al., 1996)  larval stage | third instar  dorsal mesothoracic disc (Sluss et al., 1996)  embryonic stage  embryonic epidermis (Sluss et al., 1996)  embryonic stage,larval stage,pupal stage   (Sluss et al., 1996)  embryonic stage  embryonic central nervous system (Sluss et al., 1996)  embryonic stage  embryonic peripheral nervous system (Sluss et al., 1996)    fat body (Sluss et al., 1996)  larval stage | third instar  imaginal disc (Sluss et al., 1996)  embryonic stage | early   (Sluss et al., 1996)  embryonic stage | stage >=8  embryonic peripheral nervous system (Riesgo-Escovar et al., 1996)  larval stage | third instar  eye imaginal disc (Sluss et al., 1996)  larval stage | third instar  imaginal disc (Riesgo-Escovar et al., 1996)  embryonic stage | stage 6,7  cephalic furrow (Riesgo-Escovar et al., 1996)  embryonic stage | stage >=8  embryonic central nervous system (Riesgo-Escovar et al., 1996)  embryonic stage | stage 6,7  anterior transverse furrow (Riesgo-Escovar et al., 1996)  embryonic stage | early   (Riesgo-Escovar et al., 1996)    hemocyte (Sluss et al., 1996)  embryonic stage  embryonic brain (Sluss et al., 1996)
Marker for
Subcellular Localization
CV Term
Summary of Polypeptide Expression
Stage Tissue/Position Reference
Marker for
Subcellular Localization
CV Term
External Data & Images
Linkouts	FLIGHT - Cell culture data for RNAi and other high-throughput technologies • FBgn0000229 FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array • FBgn0000229 GEO (NCBI) - Gene expression data: microarray and other high-throughput technologies • FBgn0000229 FlyExpress - Embryonic expression images (BDGP data) • Show all FlyExpress processed images for bsk Stage(s) 9-10 Stage(s) 11-12 Stage(s) 13-16
Alleles & Phenotypes
Summary of Allele Phenotypes
Lethality Allele viable bskEY01915 lethal | embryonic stage bsk1 bsk2 bskJ27 bskflp147E lethal | embryonic stage | recessive bsk1 bsk2 bskunspecified lethal, with Scer\GAL469B bskDN.Scer\UAS lethal, with Scer\GAL4e22c bskDN.Scer\UAS Other Phenotypes Allele wild-type bsk+t10 bsk+t5 visible, with Scer\GAL4GMR.PF bskDN.Scer\UAS neurophysiology defective, with Scer\GAL4elav-C155 bskK53R.Scer\UAS neuroanatomy defective | somatic clone bsk2 neuroanatomy defective, with Scer\GAL4elav-C155 bskK53R.Scer\UAS neuroanatomy defective, with Scer\GAL4ato.3.6 bskDN.Scer\UAS chemical resistant, with Scer\GAL4arm.PS bskScer\UAS.cBa chemical sensitive | dominant bsk2 tissue polarity, with Scer\GAL4hs.2sev bskK53R.Scer\UAS bskScer\UAS.cBa wound healing defective, with Scer\GAL4en-e16E bskDN.Scer\UAS wound healing defective, with Scer\GAL4A58 bskDN.Scer\UAS Sterility Allele fertile bskEY01915 Phenotype manifest in Allele embryonic/first instar larval cuticle | dorsal bsk1 bsk2 bskJ27 bskflp147E embryonic epidermis | dorsal bsk1 bsk2 bskflp147E dorsal closure embryo bsk1 embryonic/first instar larval cuticle | dorsal | germ-line clone bsk1 dorsal closure embryo | germ-line clone bsk1 embryonic dorsal epidermis bsk1 embryonic dorsal epidermis (with Df(2L)flp147E) bsk1 axon & dorsal cluster neuron | somatic clone bsk2 eye, with Scer\GAL4GMR.PF bskDN.Scer\UAS dorsal mesothoracic disc, with Scer\GAL4en-e16E bskDN.Scer\UAS embryo | dorsal, with Scer\GAL4e22c bskDN.Scer\UAS embryo | dorsal, with Scer\GAL469B bskDN.Scer\UAS thorax, with Scer\GAL4ap-md544 bskDN.Scer\UAS axon & dorsal cluster neuron, with Scer\GAL4ato.3.6 bskDN.Scer\UAS embryonic/first instar larval cuticle | dorsal, with Scer\GAL4arm.PS bskK53R.Scer\UAS embryonic leading edge cell, with Scer\GAL4arm.PS bskK53R.Scer\UAS adult thorax, with Scer\GAL4ap-md544 bskK53R.Scer\UAS mesothoracic cleft | ectopic, with Scer\GAL4ap-md544 bskK53R.Scer\UAS bouton, with Scer\GAL4elav-C155 bskK53R.Scer\UAS neuromuscular junction & larva, with Scer\GAL4elav-C155 bskK53R.Scer\UAS eye, with Scer\GAL4hs.2sev bskK53R.Scer\UAS bskScer\UAS.cBa ommatidium, with Scer\GAL4hs.2sev bskK53R.Scer\UAS bskScer\UAS.cBa eye, with Scer\GAL4αTub84B.PL bskK53R.Scer\UAS eye, with Scer\GAL4ey.PH bskScer\UAS.cBa thorax, with Scer\GAL4pnr-MD237 bskdsRNA.Scer\UAS photoreceptor cell | somatic clone bskflp147E ommatidium | somatic clone bskflp147E embryonic/first instar larval cuticle | anterior | dorsal bskunspecified
Classical Alleles ( 18 )
For All Classical Alleles Show
Allele of bsk Class Mutagen Stocks Known lesion bsk1 ethyl methanesulfonate 2 Yes bsk2 ethyl methanesulfonate 2 Yes bsk3 ethyl methanesulfonate 0 -- bskEY01915 P-element activity 1 -- bskG179 ethyl methanesulfonate 0 Yes bskG258 ethyl methanesulfonate 0 Yes bskG290 ethyl methanesulfonate 0 Yes bskG368 ethyl methanesulfonate 0 Yes bskG400 ethyl methanesulfonate 0 Yes bskG418 ethyl methanesulfonate 0 -- bskG536 ethyl methanesulfonate 0 Yes bskG577 ethyl methanesulfonate 0 Yes bskG688 ethyl methanesulfonate 0 Yes bskG760 ethyl methanesulfonate 0 Yes bskJ27 hypomorph 2 -- bske02863 piggyBac transposase 1 -- bskflp147E amorph P-element activity 0 Yes bskunspecified 0 --
Alleles Carried on Transgenic Constructs ( 17 )
For All Alleles Carried on Transgenic Constructs Show
Allele of bsk Class Mutagen Stocks Known lesion bsk+t10 in vitro construct | genomic fragment 0 Yes bsk+t5 in vitro construct | genomic fragment 0 Yes bskAct5C.T:Ivir\HA1 in vitro construct | regulatory fusion in vitro construct | coding region fusion 0 -- bskAct5C.T:Zzzz\FLAG in vitro construct | regulatory fusion in vitro construct | coding region fusion 0 Yes bskAct5C.T:Zzzz\His6 in vitro construct | regulatory fusion in vitro construct | coding region fusion 0 Yes bskDN.Scer\UAS in vitro construct | regulatory fusion 1 Yes bskGD10555 in vitro construct | RNAi in vitro construct | regulatory fusion 2 Yes bskK53R.Scer\UAS in vitro construct | regulatory fusion in vitro construct | amino acid replacement 0 Yes bskScer\UAS.cAYa in vitro construct | regulatory fusion 1 Yes bskScer\UAS.cBa in vitro construct | regulatory fusion 0 Yes bskT:Zzzz\FLAG in vitro construct | coding region fusion 0 Yes bskdsRNA.Scer\UAS in vitro construct | regulatory fusion in vitro construct | RNAi 0 Yes bskdsRNA.cCa in vitro construct | RNAi 0 Yes bskdsRNA.cIa in vitro construct | RNAi 0 Yes bskdsRNA.cPa in vitro construct | RNAi 0 Yes bskdsRNA.cSa in vitro construct | RNAi 0 Yes bskhs.PS in vitro construct | regulatory fusion 0 Yes
Aneuploid Aberrations
Useful deficiency	Df(2L)TE31Aa-1
Useful duplication	Dp(2;2)Mdh3
Disrupted in	Df(2L)BSC143 (Christensen and Cook, 2006.3.8) Df(2L)BSC144 (Christensen and Cook, 2006.3.8) Df(2L)BSC206 (Christensen and Cook, 2006.8.30) Df(2L)Exel6026 (Ryder, 2004.4.26) Df(2L)G2 (Clegg et al., 1993) Df(2L)J1 (Clegg et al., 1993) Df(2L)J106 (Clegg et al., 1993) Df(2L)J16 (Clegg et al., 1993) Df(2L)J17 (Clegg et al., 1993) Df(2L)J2 (Clegg et al., 1993) Df(2L)J27 (Tearle and Nusslein-Volhard, 1987, Clegg et al., 1993, Riesgo-Escovar et al., 1996) Df(2L)J3 (Clegg et al., 1993) Df(2L)J39 (Clegg et al., 1993) Df(2L)J77 (Clegg et al., 1993) Df(2L)flp147E (Zecchini et al., 1999, Bohni et al., 1999, Goberdhan et al., 1999, Shen and Dahmann, 2005, Sluss et al., 1996, Riesgo-Escovar and Hafen, 1997) Df(2L)flp170B (Paricio et al., 1999, Sluss et al., 1996) Df(2L)prd2.27
Transgenic Constructs & Insertions
Transgenic Constructs
	Type of construct Name Expression data characterization construct P{WAX} NA P{WX} NA heat-shock construct P{DJNK} NA UAS construct P{GD10555} NA P{UAS-bsk.A-Y} NA P{UAS-bsk.B} NA P{UAS-bsk.DN} NA P{UAS-bsk.IR}