Gene Dmel\Bsg25D

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General Information

Symbol

Dmel\Bsg25D

Species

D. melanogaster

Name

Blastoderm-specific gene 25D

Annotation symbol

CG14025

Feature type

protein_coding_gene

FlyBase ID

FBgn0000228

Created / Updated

2003-12-01/2003-12-01

Genomic Location

Chromosome (arm)

Recombination map

Cytogenetic map

25D2-25D3

Sequence location

2L:5,271,724..5,278,558 [-]

Map ( GBrowse )

Summary Information

Automatically generated summary

See sections below for more information

The gene Blastoderm-specific gene 25D is referred to in FlyBase by the symbol Bsg25D (CG14025, FBgn0000228). It has the cytological map location 25D2-25D3. Its sequence location is 2L:5271724..5278558. Its molecular function is unknown. The biological processes in which it is involved are not known. 2 alleles are reported. No phenotypic data is available. It has 3 annotated transcripts and 3 annotated polypeptides.

Detailed Mapping Data

FlyBase Computed Cytological Location

Cytogenetic map

Evidence for location

25D2-25D3

Limits computationally determined from genome sequence between P{lacW}tkv^k16713&P{lacW}l(2)k01302^k01302 and P{lacW}vri^k05901&P{PZ}cype⁰³⁷⁷¹

Experimentally Determined Cytological Location

Cytogenetic map

Notes

References

25D3-25D3

(Boyer, 1997.11.8)

25D-25D

(determined by in situ hybridisation)

(Boyer et al., 1987)

25D-25D

(determined by in situ hybridisation)

(Knust et al., 1987)

Experimentally Determined Recombination Data

Location

Left of (cM)

Right of (cM)

Notes

Molecular Map Data

Gene Order (in direction of increasing cytology)

References

In direction of increasing cytology: tkv- Bsg25D- anon-25Da? vri+ anon-25Db? anon-25Dc? cype?

(George and Terracol, 1997)

Gene Order (overall orientation not stated)

References

Gene Model & Products

Please see the GBrowse view of Dmel\Bsg25D for information on other features

Comments on Gene Model

The Bsg25D-RA gene structure is based on genomic DNA accession GB:X04896 and FBrf0047100. It is not supported by available EST and cDNA data.

Transcript Data

Annotated Transcripts

Name

FlyBase ID

RefSeq ID

Length (nt)

Associated CDS (aa)

Bsg25D-RA

FBtr0079083

NM_057568

4823

741

Bsg25D-RB

FBtr0079084

NM_164637

4599

1091

Bsg25D-RC

FBtr0079085

NM_164638

4066

1032

Additional Transcript Data & Comments

Reported size (kB)

4.5, 2.7 (northern blot)

(Boyer et al., 1987)

Comments

External Data

Crossreferences

Polypeptide Data

Annotated Polypeptides

Name

FlyBase ID

Predicted MW (kD)

Length (aa)

Theoretical pI

RefSeq ID

GenBank protein

Bsg25D-PA

FBpp0078717

84.4

741

4.44

NP_476916

AAN10537

Bsg25D-PB

FBpp0078718

122.9

1091

4.87

NP_723072

AAF52235

Bsg25D-PC

FBpp0078719

116.6

1032

5.01

NP_723073

AAN10536

Additional Polypeptide Data & Comments

Reported size (kD)

741 (aa)

(Boyer et al., 1987)

Comments

External Data

Linkouts

PANTHER - Protein classification by function, families, and pathways

•

FBgn0000228

Crossreferences

Sequences Consistent with the Gene Model

DDBJ /
EMBL /
GenBank

DNA sequence

Protein sequence

Name

P11929

UniProtKB/TrEMBL

Maps to

(FlyBase, 1992-)

Does NOT map to

Identified with

LD21844

(BDGP Project Members, 2000-)

D1151

(BDGP Project Members, 1994-1999)

Mapped Features & Mutations

Please see GBrowse or insertion reports for information on insertions of transgenic constructs and features not listed here

Type

Symbol & Location

Additional Notes

References

External Data

Linkouts

DEDB - Drosophila exon database: splicing graphs

•

6516

Crossreferences

EPD - Eukarytoic Promoter Database, an annotated collection of POL II promoters

•

23023

Expression Data

FlyBase-Curated Data

Transcript and
Protein data

Please see the FlyBase Gene Expression Report for details of gene expression from the literature.

Summary of Transcript Expression

Stage

Tissue/Position

Reference

embryonic stage | <=8 hr

(Boyer et al., 1987)

embryonic stage | blastoderm

(Boyer et al., 1987)

Marker for

Subcellular Localization

CV Term

Summary of Polypeptide Expression

Stage

Tissue/Position

Reference

Marker for

Subcellular Localization

CV Term

Microarray Data

Developmental timecourse, Costello et al., 2008 (Original data from Arbeitman et al., 2002)

Untitled Document detailed view

AA940602

External Data & Images

Linkouts

FLIGHT - Cell culture data for RNAi and other high-throughput technologies

•

FBgn0000228

FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array

•

FBgn0000228

GEO (NCBI) - Gene expression data: microarray and other high-throughput technologies

•

FBgn0000228

Alleles & Phenotypes

Summary of Allele Phenotypes

Phenotype manifest in

Allele

Classical Alleles ( 0 )

For All Classical Alleles Show

Allele of Bsg25D	Class	Mutagen	Stocks	Known lesion

Alleles Carried on Transgenic Constructs ( 2 )

For All Alleles Carried on Transgenic Constructs Show

Allele of Bsg25D	Class	Mutagen	Stocks	Known lesion
Bsg25D^GD4717		in vitro construct \| RNAi in vitro construct \| regulatory fusion	1	Yes
Bsg25D^dsRNA.cGa		in vitro construct \| RNAi	0	Yes

Aneuploid Aberrations

Disrupted in

Df(2L)Exel6011

(Ryder, 2004.4.26)

Transgenic Constructs & Insertions

Transgenic Constructs

Type of construct

Name

Expression data

UAS construct

P{GD4717}

Insertions

Type of insertions

Name

Expression data

Related Comments

Please look at the allele reports for the complete phenotype data

Gene Ontology: Function, Process & Cellular Component ( 3 )

Molecular Function

CV term

Evidence

References

molecular_function

no biological data available

(FlyBase Curators, 2002-)

Biological Process

CV term

Evidence

References

biological_process

no biological data available

(FlyBase Curators, 2002-)

Cellular Component

CV term

Evidence

References

cellular_component

no biological data available

(FlyBase Curators, 2002-)

Sequence Ontology: Class of Gene

protein_coding_gene

nuclear_gene

Interactions & Pathways

Summary of Genetic Interactions

Interacts with

Please look at the allele data for full details of the genetic interactions

Bsg25D allele

Gene

References

External Data

Linkouts

BioGRID - Interaction data, including yeast 2-hybrid and genetic interactions

•

59928

Drosophila PIMRider - The Drosophila Protein Interaction map

•

521774

Orthologs

Genome-wide drosophilid orthologs

Dana\GF15518

Dere\GG24312

Dgri\GH13539

Dmoj\GI10587

Dper\GL18560

Dpse\GA12708

Dsec\GM18030

Dsim\GD22658

Dvir\GJ21801

Dwil\GK24848

Dyak\GE19006

Curated drosophilid orthologs

Dpse\GA12708

Linkouts

InParanoid orthologs - Eukaryotic orthologs

•

ENSANGP00000009934
Anopheles gambiae (Ensembl)
ENSCAFP00000006562
Canis familiaris (Ensembl)
ENSGALP00000020163
Gallus gallus (Ensembl)
ENSP00000278886
Homo sapiens (Ensembl)
ENSMODP00000008056
Monodelphis domestica (Ensembl)
ENSRNOP00000035133
Rattus norvegicus (Ensembl)

Functional Complementation between Species

Inter-Species Misexpression Data

Produces phenotype in

Produces NO phenotype in

Stocks & Reagents

Stocks Listed in FlyBase ( 1 )

VDRC

v13998

w¹¹¹⁸; P{GD4717}v13998

Genomic Clones ( 2 )

cDNA Clones ( 46 )

cDNA Clones, Fully Sequenced

BDGP DGC clones

LD21844

Other clones

cDNA Clones, End Sequenced (ESTs)

BDGP DGC clones

Other clones

RNAi & Array Information

Affy Oligo

Linkouts

DRSC - RNAi screening (Harvard): high-throughput cell culture data and design

•

FBgn0000228

GenomeRNAi - RNAi phenotypes (Heidelberg): high-throughput cell culture data

•

FBgn0000228

Antibody Information

Other Information

Discoverer

Etymology

Identification

Position Effect Variegation Data

Relationship to Other Genes

Source for database identity of

Source for database merge of

Additional comments

Comments About Role

Comments About Molecular Function

Other Comments

In a sample of 79 genes with multiple introns, 33 showed significant heterogeneity in G+C content among introns of the same gene and significant positive correspondence between the intron and the third codon position G+C content within genes. These results are consistent with selection adding against preferred codons at the start of genes.

(Kliman and Eyre-Walker, 1998)

External Crossreferences & Linkouts

Sequence Crossreferences

RefSeq (Transcripts)

•

RefSeq (Proteins)

•

DDBJ / EMBL / GenBank

•

UniProtKB/Swiss-Prot

•

P11929

UniProtKB/TrEMBL

•

Other Crossreferences

EPD - Eukarytoic Promoter Database, an annotated collection of POL II promoters

•

23023

Linkouts

BioGRID - Interaction data, including yeast 2-hybrid and genetic interactions

•

59928

DEDB - Drosophila exon database: splicing graphs

•

6516

DRSC - RNAi screening (Harvard): high-throughput cell culture data and design

•

FBgn0000228

Drosophila PIMRider - The Drosophila Protein Interaction map

•

521774

FLIGHT - Cell culture data for RNAi and other high-throughput technologies

•

FBgn0000228

FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array

•

FBgn0000228

FlyMine - Integrated genomics database for Drosophila, Anopheles, and C.elegans

•

FBgn0000228

GEO (NCBI) - Gene expression data: microarray and other high-throughput technologies

•

FBgn0000228

GenomeRNAi - RNAi phenotypes (Heidelberg): high-throughput cell culture data

•

FBgn0000228

InParanoid orthologs - Eukaryotic orthologs

•

ENSANGP00000009934
Anopheles gambiae (Ensembl)
ENSCAFP00000006562
Canis familiaris (Ensembl)
ENSGALP00000020163
Gallus gallus (Ensembl)
ENSP00000278886
Homo sapiens (Ensembl)
ENSMODP00000008056
Monodelphis domestica (Ensembl)
ENSRNOP00000035133
Rattus norvegicus (Ensembl)

PANTHER - Protein classification by function, families, and pathways

•

FBgn0000228

University of Chicago Dev. Expression

•

FBgn0000228

Synonyms & Secondary IDs ( 6 )

Reported As

Symbol Synonym

brg25D

(Boyer et al., 1996)

bsg25D

(Szuplewski et al., 2003, Boyer, 1997.11.8, George and Terracol, 1997, Boyer et al., 1997, )

Bsg25D

(Lecuyer et al., 2007)

bsq25D

(Maroni, 1994)

CG14025

Name Synonym

Blastoderm-specific gene 25D

Secondary FlyBase IDs

References ( 21 )

Generate a list of

All references relating to this gene ( 21 )

List References by type

Recent research papers ( 1 )

Lecuyer et al., 2007, Cell 131(1): 174--187: Global analysis of mRNA localization reveals a prominent role in organizing cellular architecture and function. [FBrf0200939]

Show all research papers ...