• Home
• Tools
  • BLAST
  • GBrowse
  • QueryBuilder
  • TermLink
  • ImageBrowse
  • Interactions Browser
  • Apollo
  • CytoSearch
  • QuickSearch
  • Batch Download
  • Coordinate Converter
  • Google™ FlyBase
  • Find A Person
  • Update An Address
  • Add A New Person
• Files
  • Downloads:
  • Precomputed files
  • Archived data
  • Releases FTP
  • Genomes FTP
  • Lists:
  • Transposons (Dmel)
  • Transposons (other)
  • Vectors & Constructs
  • Anatomical Terms
  • Map Conversion
• Species
  • Phylogeny
  • Synteny Table
  • Drosophilidae
  • Abbreviations
• Documents
  • About FlyBase
  • Reference Manual:
  • Sections
  • Contents
  • Nomenclature
  • Release Notes
• Resources
  • All resources
  • Interactive Fly
  • Textpresso for Fly
  • AAA site
  • BDGP
  • DHGP
  • DGRC
  • Model Organisms
  • Stock Collections:
  • Bloomington
  • DrosDel
  • Ehime
  • Exelixis (Harvard)
  • GDP (Baylor)
  • Kyoto
  • NIG-Fly
  • Szeged
  • Tucson
  • VDRC
• News
  • News
  • FlyBase Forum
  • FlyBase Positions
  • Meetings
  • Courses
  • Fly Board
  • White papers
  • Funding
  • bionet.dros
• Help
  • Google™ FlyBase
  • Site Map
  • FAQs
  • Report help
  • New to Flies
  • Pers. comm.
  • Author guidelines
  • Known Problems
  • Contact FlyBase
• Archives
  • Prior Home Page
  • R4.3 BLAST
  • R4.3 GBrowse
  • Older Archives

Gene Dmel\baz

General Information
Symbol	Dmel\baz	Species	D. melanogaster
Name	bazooka	Annotation symbol	CG5055
Feature type	protein_coding_gene	FlyBase ID	FBgn0000163
Created / Updated	2003-12-01/2006-05-09
Genomic Location
Chromosome (arm)	X	Recombination map	1-56.7
Cytogenetic map	15F1-15F1	Sequence location	X:17,054,062..17,089,350 [+]
Map ( GBrowse )	Decorated FastA GenBankGFF Gene region Extended Gene region CDSIntronsExonsTranslationsTranscripts3' UTR5' UTR
Summary Information
Automatically generated summary See sections below for more information	The gene bazooka is referred to in FlyBase by the symbol baz (CG5055, FBgn0000163). It has the cytological map location 15F1. Its sequence location is X:17054062..17089350. Its molecular function is described as: protein kinase C binding; protein binding. It is involved in the biological processes described with 24 unique terms, many of which group under: anatomical structure development; macromolecule localization; cell morphogenesis; protein localization; cell division; establishment and/or maintenance of cell polarity; primary metabolic process; ovarian follicle cell development; cell fate commitment; gamete generation. 43 alleles are reported. The phenotypes of these alleles are annotated with 19 unique terms, many of which group under: organ system; anatomical structure; plasma membrane part; nervous system; plasma membrane; peripheral nervous system; follicle cell; germ cell; embryonic head; integumentary system; adherens junction; epithelial cell; zonula adherens; embryonic nervous system; developing anatomical structure. It has 2 annotated transcripts and 2 annotated polypeptides.
External Summaries
Phenotypic Description from the Red Book (Lindsley & Zimm 1992)
Gene/Allele symbols may differ from current usage	baz: bazooka Homozygous lethal, large dorsal and ventral hole in embryo. Homozygous baz germ-line clones undergo oogenesis, but the heterozygous progeny produced display major reductions in viability (Wieschaus and Noell).
Detailed Mapping Data
FlyBase Computed Cytological Location
Cytogenetic map Evidence for location 15F1-15F1   Limits computationally determined from genome sequence between P{EP}EP1337 and P{EP}bazEP1446
Experimentally Determined Cytological Location
Cytogenetic map Notes References 15F-15F (determined by in situ hybridisation)   (Peter et al., 2002) 15F-15F (determined by in situ hybridisation)   (Schaefer et al., 1999.12.2) 15E-15F   (Knust, 1998.11.17) 15E-15F   (Kuchinke et al., 1996)
Experimentally Determined Recombination Data
Location	1-56.7 (Tearle and Nusslein-Volhard, 1987)
Left of (cM)	f (Kuchinke et al., 1996)
Right of (cM)
Notes
Molecular Map Data
Gene Order (in direction of increasing cytology) References In direction of increasing cytology: mei-218? RpS5a? xmas-1- xmas-2? xmas-1- xmas-2? baz? (Xu, 2000.1.25) Gene Order (overall orientation not stated) References
Gene Model & Products
Please see the GBrowse view of Dmel\baz for information on other features
Comments on Gene Model
	DGC clone LD15989 appears problematic: incomplete CDS Current 3'UTR overlaps downstream blastopia{}148 transposon
Transcript Data
Annotated Transcripts
Name FlyBase ID RefSeq ID Length (nt) Associated CDS (aa) baz-RA FBtr0074388  NM_078659   6361   1464 baz-RB FBtr0111000  NM_001042818   5486   1520
Additional Transcript Data & Comments
Reported size (kB)	6.7 (northern blot) (Kuchinke et al., 1998)
Comments
External Data
Crossreferences
Polypeptide Data
Annotated Polypeptides
Name FlyBase ID Predicted MW (kD) Length (aa) Theoretical pI RefSeq ID GenBank protein baz-PA   FBpp0074162   157.4   1464   9.18     NP_523383   AAF48703 baz-PB   FBpp0110299   163.6   1520   9.17     NP_001036283   ABI30988
Additional Polypeptide Data & Comments
Reported size (kD)	1464 (aa) (Kuchinke et al., 1998)
Comments	baz protein localizes to the anterior cytoplasm of stage 3 and 5 oocytes and does not overlap with par-1 protein. (Vaccari and Ephrussi, 2002)
External Data
Linkouts	PANTHER - Protein classification by function, families, and pathways • FBgn0000163
Crossreferences	InterPro domains - A database of protein families, domains, and functional sites • PDZ/DHR/GLGF (IPR001478)
Sequences Consistent with the Gene Model
DDBJ / EMBL / GenBank	DNA sequence Protein sequence Name AA201577   AA440897   AA541050   AE003504 AAF48703   AI515401   AJ130871 CAA10224   AJ426969   AQ254775   AW942391   BQ103541   BT003584 AAO39588   CL526156   CO318173   CO319817   CO321845   CO327388   CO327934   CO331824   CO334039   CZ482275   CZ490152   EC052510   EC064432   EC064755   EC078542   EC085849   EC088641   EC090382   EC090568   EC090826   EC201999   EC203390   EC203648   EC209346   EC212265   EC212706   EC214224   EC224130   EC231784   EC244930   EC246278   EC250114   EC250563   EC252937   EC253367   EC254465   EC259877
UniProtKB/Swiss-Prot
UniProtKB/TrEMBL	O96782 Q86NX9 Q9VX75
Maps to
Does NOT map to
Identified with	LD15989 (BDGP Project Members, 2000-)
Mapped Features & Mutations
Please see GBrowse or insertion reports for information on insertions of transgenic constructs and features not listed here Type Symbol & Location Additional Notes References
External Data
Linkouts	DEDB - Drosophila exon database: splicing graphs • 3123
Crossreferences
Expression Data
FlyBase-Curated Data
Transcript and Protein data	Please see the FlyBase Gene Expression Report for details of gene expression from the literature.
Summary of Transcript Expression
Stage Tissue/Position Reference  embryonic stage  epithelial cell | apical (Kuchinke et al., 1998)  embryonic stage  neuroblast | apical (Kuchinke et al., 1998)  embryonic stage   (Kuchinke et al., 1998)
Marker for
Subcellular Localization
CV Term	cytoplasm | apical (Kuchinke et al., 1998)
Summary of Polypeptide Expression
Stage Tissue/Position Reference  adult stage | female  germ cell (Benton and St. Johnston, 2003)    stage S3 oocyte (Vaccari and Ephrussi, 2002)  embryonic stage  neuroblast | apical (Kuchinke et al., 1998)    stage S5 oocyte (Vaccari and Ephrussi, 2002)  embryonic stage  epithelial cell | apical (Kuchinke et al., 1998)
Marker for
Subcellular Localization
CV Term	cytoplasm | apical mitotic metaphase (Kuchinke et al., 1998)
Microarray Data
	Developmental timecourse, Costello et al., 2008 (Original data from Arbeitman et al., 2002) AI515401
External Data & Images
Linkouts	FLIGHT - Cell culture data for RNAi and other high-throughput technologies • FBgn0000163 FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array • FBgn0000163 GEO (NCBI) - Gene expression data: microarray and other high-throughput technologies • FBgn0000163
Alleles & Phenotypes
Summary of Allele Phenotypes
Lethality Allele viable bazCC01941 bazEY09846 lethal | recessive bazG0484 lethal | embryonic stage baz4 bazG0484 lethal | embryonic stage | recessive baz1 baz2 baz3 baz4 baz692-44 baz692-4 baz692-59 baz815-7 baz815-8 bazEH171 bazEH647b bazEH747 bazR21 bazR7 bazYD lethal | larval stage bazG0484 lethal, with Scer\GAL4Act5C.PI, bazScer\UAS.a.Sym bazScer\UAS.Sym lethal, with Scer\GAL4Act5C.PI, bazScer\UAS.Sym bazScer\UAS.a.Sym lethal, with Scer\GAL4αTub84B.PL, bazScer\UAS.a.Sym bazScer\UAS.Sym lethal, with Scer\GAL4αTub84B.PL, bazScer\UAS.Sym bazScer\UAS.a.Sym lethal, with Scer\GAL4Act5C bazGD1384 semi-lethal | embryonic stage, with Scer\GAL4mat.αTub67C.T:Hsim\VP16 bazScer\UAS.cSa.T:Avic\GFP Other Phenotypes Allele cell polarity defective baz4 cell polarity defective | somatic clone bazEH171 neuroanatomy defective baz4 Sterility Allele fertile bazEY09846 Phenotype manifest in Allele embryonic epidermis baz1 baz4 bazYD bazunspecified border follicle cell, with Scer\GAL4slbo.2.6, bazScer\UAS.Sym, bazScer\UAS.a.Sym baz4 zonula adherens baz4 bazYD adherens junction baz4 embryonic/first instar larval cuticle baz4 follicle cell | somatic clone baz4 bazEH171 border follicle cell | somatic clone baz4 bazEH171 neuroblast & pupa | somatic clone baz4 embryo | dorsal baz4 embryo | ventral baz4 bouton baz4 eye photoreceptor cell & eye disc & pupa | somatic clone baz4 eye photoreceptor cell & eye disc & pupa | somatic clone | cell non-autonomous baz4 ommatidium & adult | somatic clone baz4 eye | somatic clone baz4 photoreceptor cell & eye | somatic clone baz4 embryonic neuroblast & spindle | germ-line clone baz4 embryonic ganglion mother cell | germ-line clone baz4 epithelial cell | germ-line clone baz4 embryonic/first instar larval cuticle | germ-line clone baz4 embryonic/first instar larval cuticle | ventral baz4 embryonic head baz4 spot adherens junction baz4 egg chamber | germ-line clone baz815-8 bazunspecified oocyte & centrosome | germ-line clone baz815-8 oocyte & microtubule | germ-line clone baz815-8 embryonic epidermis & spindle baz815-8 embryonic ganglion mother cell baz815-8 embryonic central nervous system baz815-8 follicle cell | ectopic | somatic clone bazEH171 zonula adherens | somatic clone bazEH171 border follicle cell, with Scer\GAL4slbo.2.6, bazScer\UAS.a.Sym bazScer\UAS.Sym border follicle cell, with Scer\GAL4slbo.2.6, baz4, bazScer\UAS.a.Sym bazScer\UAS.Sym border follicle cell, with Scer\GAL4slbo.2.6, Scer\GAL4upd, bazScer\UAS.a.Sym bazScer\UAS.Sym border follicle cell, with Scer\GAL4slbo.2.6, bazScer\UAS.Sym bazScer\UAS.a.Sym border follicle cell, with Scer\GAL4slbo.2.6, baz4, bazScer\UAS.Sym bazScer\UAS.a.Sym border follicle cell, with Scer\GAL4slbo.2.6, Scer\GAL4upd, bazScer\UAS.Sym bazScer\UAS.a.Sym border follicle cell, with Scer\GAL4slbo.2.6 bazScer\UAS.cKa embryonic/first instar larval cuticle, with Scer\GAL4mat.αTub67C.T:Hsim\VP16 bazScer\UAS.cSa.T:Avic\GFP germ band & gastrula embryo bazYD germ band & early extended germ band embryo bazYD epithelial cell bazdsRNA.1944-3248 bazdsRNA.3623-4664 bazdsRNA.cLa oocyte | germ-line clone bazunspecified nurse cell | germ-line clone bazunspecified follicle cell | germ-line clone bazunspecified nurse cell fusome | germ-line clone bazunspecified macrochaeta, with Scer\GAL4pnr-MD237 bazGD1384
Classical Alleles ( 23 )
For All Classical Alleles Show
Allele of baz Class Mutagen Stocks Known lesion baz1 ethyl methanesulfonate 0 -- baz2 ethyl methanesulfonate 0 -- baz3 ethyl methanesulfonate 0 -- baz4 ethyl methanesulfonate 3 -- baz692-44 ethyl methanesulfonate 0 Yes baz692-4 ethyl methanesulfonate 0 -- baz692-59 ethyl methanesulfonate 0 -- baz815-7 X ray 0 -- baz815-8 X ray 0 -- bazCC01941 P-element activity 0 Yes bazEH171 ethyl methanesulfonate 2 -- bazEH647b ethyl methanesulfonate 0 -- bazEH747 ethyl methanesulfonate 0 -- bazEP1446 P-element activity 1 -- bazEY09846 P-element activity 1 -- bazG0484 P-element activity 1 -- bazR21 ethyl methanesulfonate 0 -- bazR7 ethyl methanesulfonate 0 -- bazXR-11 0 -- bazYD 0 -- baze04525 piggyBac transposase 1 -- bazf07341 piggyBac transposase 1 -- bazunspecified 0 --
Alleles Carried on Transgenic Constructs ( 20 )
For All Alleles Carried on Transgenic Constructs Show
Allele of baz Class Mutagen Stocks Known lesion bazGD1384 in vitro construct | RNAi in vitro construct | regulatory fusion 2 Yes bazMtnA.PW in vitro construct | regulatory fusion 0 Yes bazN.Scer\UAS.P\T.T:Avic\GFP in vitro construct | deletion 0 Yes bazS1085A.Scer\UAS.P\T.T:Avic\GFP in vitro construct | amino acid replacement 0 Yes bazS151A.S1085A.Scer\UAS.P\T.T:Avic\GFP in vitro construct | amino acid replacement 0 Yes bazS151A.Scer\UAS.P\T.T:Avic\GFP in vitro construct | amino acid replacement 0 Yes bazScer\UAS.P\T.T:Avic\GFP in vitro construct | regulatory fusion in vitro construct | coding region fusion 0 Yes bazScer\UAS.Sym in vitro construct | regulatory fusion in vitro construct | RNAi 0 Yes bazScer\UAS.a.Sym in vitro construct | regulatory fusion in vitro construct | RNAi 0 Yes bazScer\UAS.cKa in vitro construct | regulatory fusion 0 Yes bazScer\UAS.cSa.T:Avic\GFP in vitro construct | regulatory fusion in vitro construct | coding region fusion 0 Yes bazdsRNA.10-987 in vitro construct | RNAi 0 Yes bazdsRNA.1944-3248 in vitro construct | RNAi 0 Yes bazdsRNA.3623-4664 in vitro construct | RNAi 0 Yes bazdsRNA.cCa in vitro construct | RNAi 0 Yes bazdsRNA.cFa in vitro construct | RNAi 0 Yes bazdsRNA.cLa in vitro construct | RNAi 0 Yes bazdsRNA.cWa in vitro construct | RNAi 0 Yes bazdsRNA.cYa in vitro construct | RNAi 0 Yes bazΔN.Scer\UAS.P\T.T:Avic\GFP in vitro construct | deletion 0 Yes
Aneuploid Aberrations
Useful deficiency	Df(1)B25   Df(1)BK8
Useful duplication	Dp(1;Y)W73
Disrupted in	Df(1)BK16 (McKim et al., 1996) Df(1)BK17 (McKim et al., 1996) Df(1)BK18 (McKim et al., 1996) Df(1)BK28 (McKim et al., 1996) Df(1)BK8 (McKim et al., 1996) In(1)815-6 (McKim et al., 1996) Ts(1Lt;YLt)B17 (Muller et al., 1999) Ts(1Lt;YSt)B139 (Muller et al., 1999) Ts(1Lt;YSt)W23 (Muller et al., 1999)
Duplicated in	Dp(1;3)f+71b (Eberl and Hilliker, 1988, McKim et al., 1996) Dp(1;4)fK27 (McKim et al., 1996) Dp(1;4)r+l (Wieschaus et al., 1984, Eberl and Hilliker, 1988, McKim et al., 1996) Dp(1;4)rK20 (McKim et al., 1996) Dp(1;Y)W73 (McKim et al., 1996)
Not disrupted in	Df(1)B (McKim et al., 1996) Df(1)BK10 (McKim et al., 1996) Df(1)r-D17 (Tearle and Nusslein-Volhard, 1987) Dp(1;4)exd82b26c (Tearle and Nusslein-Volhard, 1987)
Not duplicated in	Dp(1;4)fK24 (McKim et al., 1996) Dp(1;4)fK4 (McKim et al., 1996) Dp(1;4)fK7 (McKim et al., 1996) Dp(1;Y)BSsog+y+ (Wickline and Lindsley, 1997)
Transgenic Constructs & Insertions
Transgenic Constructs
	Type of construct Name Expression data UAS construct P{GD1384} NA P{Sym-baz} NA P{UAS-baz.GFP.S} NA P{UAS-baz.K} NA P{UASp-baz.GFP} NA P{UASp-baz.N.GFP} NA P{UASp-baz.S1085A} NA P{UASp-baz.S151A.S1085A} NA P{UASp-baz.S151A} NA P{UASp-baz.ΔN.GFP} NA
Insertions
	Type of insertions Name Expression data miscellaneous insertions PBac{RB}baze04525 NA PBac{WH}bazf07341 NA P{PTT-GC}bazCC01941 NA insertion of enhancer trap P{lacW}bazG0484 No P{lacW}l(1)G0313G0313 No P{lacW}l(1)G0497G0497 No insertion of mobile activating element P{EPgy2}bazEY09846 NA P{EP}bazEP1446 NA