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Gene Dmel\aub

General Information
Symbol	Dmel\aub	Species	D. melanogaster
Name	aubergine	Annotation symbol	CG6137
Feature type	protein_coding_gene	FlyBase ID	FBgn0000146
Created / Updated	2003-12-01/2003-12-01
Genomic Location
Chromosome (arm)	2L	Recombination map	2-39
Cytogenetic map	32C1-32C1	Sequence location	2L:10,997,816..11,001,489 [-]
Map ( GBrowse )	Decorated FastA GenBankGFF Gene region Extended Gene region CDSIntronsExonsTranslationsTranscripts3' UTR5' UTR
Summary Information
Automatically generated summary See sections below for more information	The gene aubergine is referred to in FlyBase by the symbol aub (CG6137, FBgn0000146). It has the cytological map location 32C1. Its sequence location is 2L:10997816..11001489. Its molecular function is described as RNA binding. It is involved in the biological processes described with 16 unique terms, many of which group under: anterior/posterior axis specification; gamete generation; regulation of metabolic process; oocyte axis determination; gene silencing by RNA; cell division; cellular macromolecule metabolic process; oocyte anterior/posterior axis determination; organelle organization and biogenesis; pole cell development; establishment and/or maintenance of chromatin architecture; positive regulation of cellular biosynthetic process; anatomical structure development; karyosome formation; regulation of cellular component organization and biogenesis. 22 alleles are reported. The phenotypes of these alleles are annotated with: embryo; dorsal appendage; spermatocyte; spermatid; karyosome. It has 2 annotated transcripts and 2 annotated polypeptides.
External Summaries
Phenotypic Description from the Red Book (Lindsley & Zimm 1992)
Gene/Allele symbols may differ from current usage	aub: aubergine (T. Schupbach) Female sterile; homozygous females lay eggs which are of variable shapes; in the most extreme cases, the eggs are longer than the normal, more pointed at the posterior end, and lack dorsal appendages, resembling eggs produced by the dominant female-sterile mutation Fs(2)G.
Detailed Mapping Data
FlyBase Computed Cytological Location
Cytogenetic map Evidence for location 32C1-32C1   Limits computationally determined from genome sequence between P{lacW}l(2)k05812k05812&P{lacW}l(2)k09104k09104 and P{EP}ppoEP2478&P{lacW}l(2)k15817k15817
Experimentally Determined Cytological Location
Cytogenetic map Notes References 32D-32D   (Schaefer, 1995.12.20)
Experimentally Determined Recombination Data
Location	2-39
Left of (cM)
Right of (cM)	P{NM}31E (Harris and Macdonald, 2001)
Notes
Molecular Map Data
Gene Order (in direction of increasing cytology) References Gene Order (overall orientation not stated) References Overall orientation not stated: RpL9+ aub+ (Schmidt et al., 1999, Schaefer, 1995.12.20)
Gene Model & Products
Please see the GBrowse view of Dmel\aub for information on other features
Comments on Gene Model
	EST data support existence of multiple transcripts
Transcript Data
Annotated Transcripts
Name FlyBase ID RefSeq ID Length (nt) Associated CDS (aa) aub-RA FBtr0080165  NM_057386   2841   866 aub-RC FBtr0112793  NM_001103674   2707   795
Additional Transcript Data & Comments
Reported size (kB)	3.0 (northern blot) (Schmidt et al., 1999)
Comments
External Data
Crossreferences
Polypeptide Data
Annotated Polypeptides
Name FlyBase ID Predicted MW (kD) Length (aa) Theoretical pI RefSeq ID GenBank protein aub-PA   FBpp0079754   98.6   866   9.65     NP_476734   AAF53046 aub-PC   FBpp0111705   91.5   795   9.53     NP_001097144   ABV53667
Additional Polypeptide Data & Comments
Reported size (kD)	866 (aa); 99 (kD predicted) (Schmidt et al., 1999)
Comments
External Data
Linkouts	PANTHER - Protein classification by function, families, and pathways • FBgn0000146
Crossreferences	InterPro domains - A database of protein families, domains, and functional sites • Polynucleotidyl transferase, Ribonuclease H fold (IPR012337) Argonaute and Dicer protein, PAZ (IPR003100) Stem cell self-renewal protein Piwi (IPR003165)
Sequences Consistent with the Gene Model
DDBJ / EMBL / GenBank	DNA sequence Protein sequence Name AA440254   AA696564   AA736056   AA817492   AA817640   AA940831   AA952026   AC005116   AC092226   AE003630 AAF53046   AF145680 AAD38655   AF334408 AAK43547   AI455313   AI456065   AI541648   AI542906   AI544388   BF486974   BF488645   BF494972   BF494990   BF502146   BF505137   BG641173   BH809487   BI163041   BI166408   BI168219   BI169070   BI353998   BI356173   BI632822   BI639204   BI639215   BP546255   CZ466890   CZ473849   CZ473850   LD23107   X94613 CAA64320
UniProtKB/Swiss-Prot
UniProtKB/TrEMBL	O76922 Q95PE5
Maps to	DS01831 FBcl0297519 (FlyBase, 1992-)
Does NOT map to
Identified with	LD23107 (Rubin, 1999.4.23, BDGP Project Members, 2000-) LD23087 (Harris and Macdonald, 2001)
Mapped Features & Mutations
Please see GBrowse or insertion reports for information on insertions of transgenic constructs and features not listed here Type Symbol & Location Additional Notes References deletion aub[N11] 2L:10,998,158..10,998,311 comment=Location of a 154bp deletion beginning in codon G741, which leads to a frameshift and early translation termination. The resulting predicted protein has 16 novel amino acids added after residue 740. evidence=experimental (Harris and Macdonald, 2001, Harris, 2005.5.26) insertion site P{lacW}aub[sting-1] 2L:11,001,470..11,001,471 evidence=experimental (Schmidt et al., 1996, Schmidt et al., 1999) point mutation aub[K86] 2L:10,999,588..10,999,588 comment=Site of nucleotide substitution in mutant inferred by FlyBase based on reported amino acid change. evidence=experimental na_change=C10999588T pr_change=Q377|aub-PA,Q306|aub-PC reported_pr_change=Q377@ (Harris and Macdonald, 2001) point mutation aub[HN] 2L:10,998,669..10,998,669 comment=Site of nucleotide substitution in mutant inferred by FlyBase based on reported amino acid change. evidence=experimental na_change=C10998669T pr_change=Q622|aub-PA,Q551|aub-PC reported_pr_change=Q622@ (Harris and Macdonald, 2001) rescue fragment aub[tMR-2] 2L:10,996,675..11,003,662 comment=rescue also demonstrated with fragment 1kb shorter at 5' end (see aub[tMR-3]). 5' site is from plasmid and could not be located on genome sequence. evidence=experimental linked_to=SstII-XbaI_rfrag (Schmidt et al., 1999)
External Data
Linkouts	DEDB - Drosophila exon database: splicing graphs • 7269
Crossreferences
Expression Data
FlyBase-Curated Data
Transcript and Protein data	Please see the FlyBase Gene Expression Report for details of gene expression from the literature.
Summary of Transcript Expression
Stage Tissue/Position Reference  adult stage | female  ovary (Schmidt et al., 1999)  adult stage | male  testic (Schmidt et al., 1999)
Marker for
Subcellular Localization
CV Term
Summary of Polypeptide Expression
Stage Tissue/Position Reference
Marker for
Subcellular Localization
CV Term
External Data & Images
Linkouts	FLIGHT - Cell culture data for RNAi and other high-throughput technologies • FBgn0000146 FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array • FBgn0000146 GEO (NCBI) - Gene expression data: microarray and other high-throughput technologies • FBgn0000146
Alleles & Phenotypes
Summary of Allele Phenotypes
Lethality Allele lethal | maternal effect | recessive aubAHN aubAWE13 aubHM23 aubHN aubK86 aubN11 aubOC aubQC42 lethal | embryonic stage | non-rescuable maternal effect | recessive aubsting-3a Other Phenotypes Allele wild-type aubtMR-1 aubtMR-2 aubtMR-3 suppressor of variegation aubQC42 aubΔP-3a suppressor of variegation, with P{T1}T190-177 aubQC42 suppressor of variegation, with P{T1(-FRT)}T190-177 aubQC42 Sterility Allele female sterile aubAHN aubAWE13 aubHM23 aubHN aubOC aubQC42 female sterile | recessive aubsting-3a male sterile (with aubsting-1) aubsting-3a male sterile (with aubsting-3a) aubsting-1 male sterile | recessive aubsting-1 Phenotype manifest in Allele embryo | maternal effect | posterior aubAHN aubAWE13 aubHM23 aubHN aubK86 aubN11 aubQC42 dorsal appendage (with aubQC42) aubHN dorsal appendage (with aubHN) aubQC42 spermatocyte aubsting-1 spermatid aubsting-1 spermatocyte (with aubsting-3a) aubsting-1 spermatid (with aubsting-3a) aubsting-1 spermatocyte (with aubsting-1) aubsting-3a spermatid (with aubsting-1) aubsting-3a karyosome aubunspecified
Classical Alleles ( 16 )
For All Classical Alleles Show
Allele of aub Class Mutagen Stocks Known lesion aubAHN ethyl methanesulfonate 0 -- aubAWE13 ethyl methanesulfonate 0 -- aubHM23 ethyl methanesulfonate 0 -- aubHN56 0 -- aubHN ethyl methanesulfonate 1 Yes aubK86 ethyl methanesulfonate 0 Yes aubKG05389 P-element activity 1 -- aubN11 ethyl methanesulfonate 0 Yes aubOC ethyl methanesulfonate 0 -- aubQC42 ethyl methanesulfonate 1 -- aubc01100 piggyBac transposase 1 -- aubd04301 P-element activity 1 -- aubsting-1 P-element activity 0 Yes aubsting-3a Δ2-3 0 Yes aubunspecified 0 -- aubΔP-3a 0 --
Alleles Carried on Transgenic Constructs ( 6 )
For All Alleles Carried on Transgenic Constructs Show
Allele of aub Class Mutagen Stocks Known lesion aubGD11831 in vitro construct | RNAi in vitro construct | regulatory fusion 2 Yes aubScer\UAS.P\T.T:Avic\GFP-m6 in vitro construct | regulatory fusion in vitro construct | coding region fusion 0 Yes aubdsRNA.cDa in vitro construct | RNAi 0 Yes aubtMR-1 in vitro construct | genomic fragment 0 Yes aubtMR-2 in vitro construct | genomic fragment 0 Yes aubtMR-3 in vitro construct | genomic fragment 0 Yes
Aneuploid Aberrations
Useful duplication	Dp(2;2)Mdh3
Disrupted in	Df(2L)aubsting-3a (Schmidt et al., 1999)
Transgenic Constructs & Insertions
Transgenic Constructs
	Type of construct Name Expression data characterization construct P{aubtMR-1} NA P{aubtMR-2} NA P{aubtMR-3} NA reporter construct P{aub-lacZ.1} Yes P{aub-lacZ.2} No UAS construct P{GD11831} NA P{UASp-GFP-aub.H} NA
Insertions
	Type of insertions Name Expression data insertion of mobile activating element P{XP}aubd04301 NA miscellaneous insertions PBac{PB}aubc01100 NA P{SUPor-P}aubKG05389 NA insertion of enhancer trap P{lacW}aubsting-1 No
Related Comments
	Please look at the allele reports for the complete phenotype data Mutant alleles are female sterile, laying spindle shaped eggs with defective dorsal appendages; if fertilised the embryos show a posterior group phenotype.   Mutations at the aub locus cause defects in midoogenesis. (Schupbach and Wieschaus, 1991) aub mutants block RNAi activation which normally occurs during egg maturation. (Kennerdell et al., 2002) Mutations in piwi or aub result in partial loss of the methyl-K9 form of His3, most evident at minor sites within the euchromatic arms. (Pal-Bhadra et al., 2004)
Gene Ontology: Function, Process & Cellular Component ( 19 )
Molecular Function
CV term Evidence References
RNA binding inferred from direct assay (Brennecke et al., 2007)
Biological Process
CV term Evidence References
RNA interference traceable author statement (Cerutti, 2003) inferred from mutant phenotype (Tomari et al., 2004) traceable author statement (Lavorgna et al., 2004)
RNA interference, targeting of mRNA for destruction inferred from mutant phenotype (Kennerdell et al., 2002)
chromatin silencing inferred from mutant phenotype (Pal-Bhadra et al., 2004) inferred from mutant phenotype (Grimaud et al., 2006)
cystoblast division inferred from sequence or structural similarity with EMBL:AF104260 (FlyBase, 1992-)
dorsal appendage formation inferred from mutant phenotype (Schupbach and Wieschaus, 1991) inferred from mutant phenotype (Klattenhoff et al., 2007)
karyosome formation traceable author statement (Johnstone and Lasko, 2001)
maintenance of pole plasm mRNA location traceable author statement (Johnstone and Lasko, 2001)
oocyte maturation inferred from mutant phenotype (Kennerdell et al., 2002)
oogenesis inferred from mutant phenotype (Schupbach and Wieschaus, 1991) traceable author statement (van Eeden and St. Johnston, 1999)
pole cell formation inferred from genetic interaction with FLYBASE:osk; FB:FBgn0003015 (Harris and Macdonald, 2001)
pole plasm protein localization inferred from mutant phenotype (Klattenhoff et al., 2007)
positive regulation of oskar mRNA translation traceable author statement (Mahowald, 2001) non-traceable author statement (Leatherman and Jongens, 2003)
regulation of oskar mRNA translation traceable author statement (van Eeden and St. Johnston, 1999) traceable author statement (Johnstone and Lasko, 2001)
regulation of pole plasm oskar mRNA localization inferred from mutant phenotype (Cook et al., 2004)
regulation of translational initiation inferred from sequence or structural similarity with EMBL:AF005355; protein_id:AAC24323.1 (Harris and Macdonald, 2001)
stem cell division inferred from sequence or structural similarity with EMBL:AF104260 (FlyBase, 1992-)
Cellular Component
CV term Evidence References
cytoplasm inferred from direct assay (Brennecke et al., 2007) inferred from direct assay (Harris and Macdonald, 2001)
polar granule inferred from direct assay (Harris and Macdonald, 2001)
Sequence Ontology: Class of Gene
	protein_coding_gene   nuclear_gene
Interactions & Pathways
Summary of Genetic Interactions
Interacts with	Please look at the allele data for full details of the genetic interactions aub allele Gene References aubAHN osk (Wilson et al., 1996) aubAWE13 osk (Wilson et al., 1996, Wilson et al., 1996) aubHM23 osk (Wilson et al., 1996, Wilson et al., 1996) aubHN56 osk (Wilson et al., 1996) aubHN osk (Wilson et al., 1996, Wilson et al., 1996, Harris and Macdonald, 2001) aubN11 osk (Harris and Macdonald, 2001) aubsting-1 Ste (Schmidt et al., 1999, Tritto et al., 2003) unspecified mei-41 (Klattenhoff et al., 2007) lok (Klattenhoff et al., 2007) osk (Wilson et al., 1996, Wilson et al., 1996)
External Data
Linkouts	BioGRID - Interaction data, including yeast 2-hybrid and genetic interactions • 60589
Orthologs
Genome-wide drosophilid orthologs	Dere\GG10336 Dgri\GH10973 Dmoj\GI18157 Dpse\GA19382 Dsec\GM11225 Dsim\GD22205 Dvir\GJ14585 Dwil\GK15249 Dyak\GE13099
Curated drosophilid orthologs	Dpse\GA19382
Linkouts	InParanoid orthologs - Eukaryotic orthologs • ENSANGP00000011087 Anopheles gambiae (Ensembl) ENSBTAP00000039920 Bos taurus (Ensembl) ENSCAFP00000010128 Canis familiaris (Ensembl) ENSCINP00000019672 Ciona intestinalis (Ensembl) ENSGALP00000004162 Gallus gallus (Ensembl) ENSP00000245255 Homo sapiens (Ensembl) ENSMMUP00000007441 Macaca mulatta (Ensembl) ENSMMUP00000011747 Macaca mulatta (Ensembl) ENSMODP00000019498 Monodelphis domestica (Ensembl) ENSPTRP00000009580 Pan troglodytes (Ensembl) ENSRNOP00000001238 Rattus norvegicus (Ensembl) ENSXETP00000027524 Xenopus tropicalis (Ensembl) WBGene00032975 Caenorhabditis briggsae (Wormbase) WBGene00008416 Caenorhabditis elegans (Wormbase) ZDB-GENE-030813-2 Danio rerio (Zfin) DDB0220438 Dictyostelium discoideum (Dictybase) MGI:1928897 Mus musculus (Informatics)
Functional Complementation between Species
Inter-Species Misexpression Data
Produces phenotype in
Produces NO phenotype in
Stocks & Reagents
Stocks Listed in FlyBase ( 7 )
Harvard	- P{XP}aubd04301 - PBac{PB}aubc01100
VDRC	v30124 w1118; P{GD11831}v30124 v30125 w1118; P{GD11831}v30125
Bloomington	8517 aubHN cn1 bw1/CyO 14001 y1 w67c23; P{SUPor-P}aubKG05389 4968 w1118; aubQC42 cn1 bw1/CyO, P{sevRas1.V12}FK1
Genomic Clones ( 2 )
	BACR20O15 BACR03D06
cDNA Clones ( 26 )
cDNA Clones, Fully Sequenced
BDGP DGC clones
Other clones
cDNA Clones, End Sequenced (ESTs)
BDGP DGC clones	LD29041 AT24134 AT03764 GM07892 RE06526 GM25891 SD26696 SD21722 RE10044 SD10930 AT17786 RE02337 SD05903 LD35892 AT21591 SD21739 LD14893 RE08810 LD24150 AT07309 RE42255 AT03787 SD12449 SD09421