Gene Dmel\asp

(Shimell et al., 1991)

Left of (cM)

Right of (cM)

Notes

Mapping based on 64 hh^bar3-tx recombinants.

Mapping based on 64 recombinant chromosomes in the asp region.

Molecular Map Data

Gene Order (in direction of increasing cytology)

References

In direction of increasing cytology: tld? asp?

(Shimell et al., 1991)

In direction of increasing cytology: tld+ asp+

Gene Order (overall orientation not stated)

References

Gene Model & Products

Please see the GBrowse view of Dmel\asp for information on other features

Comments on Gene Model

DGC clone LD39479 appears problematic: incomplete CDS

Transcript Data

Annotated Transcripts

Name

FlyBase ID

RefSeq ID

Length (nt)

Associated CDS (aa)

asp-RA

FBtr0084692

NM_079764

6284

1954

Additional Transcript Data & Comments

Reported size (kB)

Comments

External Data

Crossreferences

Polypeptide Data

Annotated Polypeptides

Name

FlyBase ID

Predicted MW (kD)

Length (aa)

Theoretical pI

RefSeq ID

GenBank protein

asp-PA

FBpp0084071

230.2

1954

11.41

NP_524488

AAF56330

Additional Polypeptide Data & Comments

Reported size (kD)

1861 (aa); 220 (kD observed)

(BDGP Project Members, 2000-, Wainman, 2003.11.7)

Comments

External Data

Linkouts

PANTHER - Protein classification by function, families, and pathways

•

FBgn0000140

Crossreferences

InterPro domains - A database of protein families, domains, and functional sites

•

IQ calmodulin-binding region (IPR000048)

Calponin-like actin-binding (IPR001715)

Calponin-homology (IPR016146)

Sequences Consistent with the Gene Model

DDBJ /
EMBL /
GenBank

DNA sequence

Protein sequence

Name

Q9VC45

UniProtKB/TrEMBL

Maps to

Does NOT map to

Identified with

LD39479

Mapped Features & Mutations

Please see GBrowse or insertion reports for information on insertions of transgenic constructs and features not listed here

Type

Symbol & Location

Additional Notes

References

rescue fragment

asp[+t19]
3R:20,569,906..20,589,128

comment=Position of restriction fragment on reference sequence inferred by FlyBase curator.
evidence=experimental
linked_to=SalI-SalI_rfrag

External Data

Linkouts

DEDB - Drosophila exon database: splicing graphs

•

10695

Crossreferences

Expression Data

FlyBase-Curated Data

Transcript and
Protein data

Please see the FlyBase Gene Expression Report for details of gene expression from the literature.

Summary of Transcript Expression

Stage

Tissue/Position

Reference

embryonic stage,larval stage,adult stage

Show all BDGP original images, annotations and embryonic microarray data for asp

Marker for

Subcellular Localization

CV Term

Summary of Polypeptide Expression

Stage

Tissue/Position

Reference

Marker for

Subcellular Localization

CV Term

Microarray Data

Developmental timecourse, Costello et al., 2008 (Original data from Arbeitman et al., 2002)

Untitled Document detailed view

AI519740

External Data & Images

Linkouts

FLIGHT - Cell culture data for RNAi and other high-throughput technologies

•

FBgn0000140

FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array

•

FBgn0000140

GEO (NCBI) - Gene expression data: microarray and other high-throughput technologies

•

FBgn0000140

BDGP expression data

•

FlyExpress - Embryonic expression images (BDGP data)

•

Show all FlyExpress processed images for asp

Alleles & Phenotypes

Summary of Allele Phenotypes

Lethality

Allele

viable

viable (with Df(3R)Hd^γ1)

viable | male (with asp^E3)

asp^L1

viable | male (with asp^L1)

asp^E3

lethal

lethal | maternal effect | recessive

lethal | recessive

lethal | larval stage | recessive

asp¹

semi-lethal | recessive

Other Phenotypes

Allele

wild-type

asp^+t19

visible

visible | recessive

meiotic cell cycle defective | recessive

asp¹

meiotic cell cycle defective | male

meiotic cell cycle defective | female

asp^DD4

mitotic cell cycle defective

asp^E3

mitotic cell cycle defective | recessive

cytokinesis defective

asp¹

Sterility

Allele

semi-sterile (with asp^E3)

asp^L1

semi-sterile (with asp^L1)

asp^E3

female sterile

female sterile (with Df(3R)Hd^γ1)

female sterile | recessive

asp¹

female semi-sterile

male sterile

male sterile | recessive

asp¹

male sterile | recessive | conditional - cold sensitive

asp¹

Phenotype manifest in

Allele

spermatocyte & spindle (with asp^DD4)

asp¹

spermatocyte & aster (with asp^DD4)

asp¹

ovariole (with asp^DD4)

asp¹

egg chamber (with asp^DD4)

asp¹

oocyte nucleus (with asp^DD4)

asp¹

spermatocyte & spindle (with Df(3R)Hd^γ1)

asp¹

spermatocyte & spindle

centrosome

asp¹

microtubule organizing center

larval brain & neuroblast & centrosome

asp¹

larval brain & neuroblast & mitotic cell cycle

asp¹

larval brain & neuroblast & aster

asp¹

larval brain & neuroblast & spindle

asp¹

Nebenkern

asp¹

onion stage spermatid & nucleus

asp¹

aster | male

asp¹

spermatocyte & centrosome

asp¹

meiotic telophase II & spindle

asp¹

meiotic telophase II & contractile ring

asp¹

asp¹

wing

eye

spermatid

asp¹

spindle

asp¹

germline cyst

larval brain

macrochaeta

spermatocyte & spindle (with asp¹)

asp^DD4

spermatocyte & aster (with asp¹)

asp^DD4

ovariole (with asp¹)

asp^DD4

egg chamber (with asp¹)

asp^DD4

oocyte nucleus (with asp¹)

asp^DD4

germarium

asp^DD4

germarium & pupa

contractile ring | male

asp^DD4

spermatocyte ring canal

asp^DD4

oocyte

asp^DD4

mitotic cell cycle

asp^E3

spindle | maternal effect

spermatocyte (with asp^L1)

asp^E3

spermatocyte & microtubule organizing center (with asp^L1)

asp^E3

spermatocyte & microtubule (with asp^L1)

asp^E3

spindle | ectopic (with asp^L1)

asp^E3

anaphase & condensed nuclear chromosome (with asp^L1)

asp^E3

spermatocyte (with asp^E3)

asp^L1

spermatocyte & microtubule organizing center (with asp^E3)

asp^L1

spermatocyte & microtubule (with asp^E3)

asp^L1

spindle | ectopic (with asp^E3)

asp^L1

anaphase & condensed nuclear chromosome (with asp^E3)

asp^L1

germarium & spindle

asp^unspecified

germarium & microtubule

asp^unspecified

nurse cell ring canal

asp^unspecified

Classical Alleles ( 16 )

For All Classical Alleles Show

Allele of asp	Class	Mutagen	Stocks	Known lesion
asp¹	hypomorph	ethyl methanesulfonate	3	Yes
asp²³⁶		piggyBac transposase	0	Yes
asp^68-62		P-element activity	0	Yes
asp^DD1		ethyl methanesulfonate	0	--
asp^DD2		ethyl methanesulfonate	0	--
asp^DD3		ethyl methanesulfonate	0	--
asp^DD4	hypomorph	ethyl methanesulfonate	0	--
asp^DD5		ethyl methanesulfonate	0	--
asp^DD6		ethyl methanesulfonate	0	--
asp^DD7		ethyl methanesulfonate	0	--
asp^DD8		ethyl methanesulfonate	0	--
asp^DD9		ethyl methanesulfonate	0	--
asp^E3	hypomorph	X ray	0	--
asp^L18		X ray	0	--
asp^L1		X ray	0	--
asp^unspecified			0	--

Alleles Carried on Transgenic Constructs ( 3 )

For All Alleles Carried on Transgenic Constructs Show

Allele of asp	Mutagen	Stocks	Known lesion
asp^+t19	in vitro construct \| genomic fragment	0	Yes
asp^GD1375	in vitro construct \| RNAi in vitro construct \| regulatory fusion	2	Yes
asp^t12	in vitro construct \| genomic fragment	0	Yes

Aneuploid Aberrations

Useful deficiency

Df(3R)XTA1

Useful duplication

Dp(3;3)M95A⁺13

Dp(3;3)M96C⁺9

Disrupted in

Df(3R)Hd^γ1

(Riparbelli et al., 2002, White-Cooper et al., 1996, Saunders et al., 1997)

Df(3R)L16

Df(3R)Su9

Df(3R)XTA1

Df(3R)slo8

Df(3R)tld68-62

(Nguyen et al., 1994)

Ts(YSt;3Lt)A117+Ts(YSt;3Rt)B197

Duplicated in

Dp(3;3)M96C⁺9

(Carmena et al., 1991)

Ts(YSt;3Lt)A117

Not disrupted in

Df(3R)crb87-4

Df(3R)crb87-5

In(3R)Ubx^7LLats^R

(Gonzalez et al., 1989, Cobb, 1996, Saunders et al., 1997)

T(Y;3)A117

Not duplicated in

Ts(YSt;3Rt)B197

(White-Cooper et al., 1996)

Transgenic Constructs & Insertions

Transgenic Constructs

Type of construct

Name

Expression data

UAS construct

P{GD1375}

characterization construct

P{tld⁺12}

P{tld⁺19}

Insertions

Type of insertions

Name

Expression data

miscellaneous insertions

PBac{3xP3-EYFP,p-tTA-K10}asp²³⁶

Related Comments

Please look at the allele reports for the complete phenotype data

RNAi screen using dsRNA made from templates generated with primers directed against this gene results in kinetochore-fiber unfocusing and centrosome detachment phenotypes when assayed in S2 cells.

(Goshima et al., 2007)

Mutants are larval lethal, with a high frequency of aberrant (e.g. polyploid) cells arrested in metaphase in the larval brain. Postmeiotic stages of spermatogenesis are abnormal, with, e.g. multinucleate spermatids. In the absence of maternal product show severe nuclear cycle defects with free centrosomes.

Immunocytological techniques have been used to observe the spindle apparatus in asp mutants and the results indicate that non-disjunction in asp can result from the association of condensed chromosomes with hemi-spindle structures in meiosis and mitosis.

(Gonzalez et al., 1990)

The hypothesis that most mitotic mutants do not lead to lethality in early development due to the effect of maternally provided wild type products has been tested by studying cuticular clones.

(Carmena et al., 1991)

Monopolar spindles in asp mutants are hemispindles in which an asymmetric array of microtubules extend from a single centrosome towards condensed chromosomes.

(Glover, 1995)

Five genes essential for cell cycle progression, polo, mgr, asp, stg and gnu, are recombined onto a single chromosome, EMS mutations are isolated that fail to complement this chromosome. Genes encoding proteins that interact with the products of any of the five genes are isolated.

RNAi screen using dsRNA made from templates generated with primers directed against this gene results in unfocused spindle microtubules and pole detachment when assayed in S2 cells. This phenotype can be observed when the screen is performed with or without Cdc27 dsRNA.

(Goshima et al., 2007)

Mitotic S2 cells treated with dsRNA made from templates generated with primers directed against this gene display a striking detachment of centrosomes from spindles and a loss of spindle pole focusing, resulting in an increase in pole-pole spacing. There is also an increase in spindle length and an elevation of the mitotic index.

(Morales-Mulia and Scholey, 2005)

Gene Ontology: Function, Process & Cellular Component ( 19 )

Molecular Function

CV term

Evidence

References

microtubule binding

inferred from direct assay