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Gene Dmel\arr

General Information
Symbol	Dmel\arr	Species	D. melanogaster
Name	arrow	Annotation symbol	CG5912
Feature type	protein_coding_gene	FlyBase ID	FBgn0000119
Created / Updated	2003-12-02/2003-12-02
Genomic Location
Chromosome (arm)	2R	Recombination map	2-66
Cytogenetic map	50A9-50A11	Sequence location	2R:9,341,138..9,370,723 [-]
Map ( GBrowse )	Decorated FastA GenBankGFF Gene region Extended Gene region CDSIntronsExonsTranslationsTranscripts3' UTR5' UTR
Summary Information
Automatically generated summary See sections below for more information	The gene arrow is referred to in FlyBase by the symbol arr (CG5912, FBgn0000119). It has the cytological map location 50A9-50A11. Its sequence location is 2R:9341138..9370723. Its molecular function is described as: low-density lipoprotein receptor activity; protein binding; Wnt receptor activity. It is involved in the biological processes: Wnt receptor signaling pathway; protein targeting to lysosome; compound eye morphogenesis; regulation of compound eye pigmentation. 37 alleles are reported. The phenotypes of these alleles are annotated with 23 unique terms, many of which group under: organ system; peripheral nervous system; nervous system; cuticle; adult segment; integumentary system; adult mesothoracic segment; embryonic nervous system; imaginal precursor; thoracic segment; dorsal thoracic disc; midgut constriction. It has one annotated transcript and one annotated polypeptide.
External Summaries
Phenotypic Description from the Red Book (Lindsley & Zimm 1992)
Gene/Allele symbols may differ from current usage	arr: arrow Embryonic lethal. Additional denticle bands anterior and posterior to normal denticle bands, especially in ventral midline. Strongest at 18.
Detailed Mapping Data
FlyBase Computed Cytological Location
Cytogenetic map Evidence for location 50A9-50A11   Limits computationally determined from genome sequence between P{EP}DpEP358 and P{lacW}drkk13809&P{EP}mip120EP606
Experimentally Determined Cytological Location
Cytogenetic map Notes References 50A9-50A10   (Avery, 2000.1.11) 50A9-50A10   (BDGP Project Members, 1994-1999)
Experimentally Determined Recombination Data
Location	2-66 (Tearle and Nusslein-Volhard, 1987)
Left of (cM)
Right of (cM)	cnn (Vaizel-Ohayon and Schejter, 1999)
Notes
Molecular Map Data
Gene Order (in direction of increasing cytology) References Gene Order (overall orientation not stated) References Overall orientation not stated: arr? cid? drk? (Ahmad, 2000.3.13, Ahmad, 2000.3.3)
Gene Model & Products
Please see the GBrowse view of Dmel\arr for information on other features
Comments on Gene Model
	DGC clone LD10754 appears problematic: incomplete CDS
Transcript Data
Annotated Transcripts
Name FlyBase ID RefSeq ID Length (nt) Associated CDS (aa) arr-RA FBtr0087702  NM_079998   6020   1678
Additional Transcript Data & Comments
Reported size (kB)
Comments
External Data
Crossreferences
Polypeptide Data
Annotated Polypeptides
Name FlyBase ID Predicted MW (kD) Length (aa) Theoretical pI RefSeq ID GenBank protein arr-PA   FBpp0086821   187.5   1678   7.00     NP_524737   AAF58373
Additional Polypeptide Data & Comments
Reported size (kD)
Comments
External Data
Linkouts	PANTHER - Protein classification by function, families, and pathways • FBgn0000119
Crossreferences	InterPro domains - A database of protein families, domains, and functional sites • Low-density lipoprotein receptor, YWTD repeat (IPR000033) Aspartic acid and asparagine hydroxylation site (IPR000152) Low density lipoprotein-receptor, class A, cysteine-rich (IPR002172) EGF-like (IPR006209) EGF (IPR006210) Six-bladed beta-propeller, TolB-like (IPR011042) Low density lipoprotein receptor-related protein, 5/6 (IPR017049)
Sequences Consistent with the Gene Model
DDBJ / EMBL / GenBank	DNA sequence Protein sequence Name AA140895   AA391829   AE003818 AAF58373   AF223365 AAF28358   AW942154   AY005815 AAF91072   BT015250 AAT94479   CO184554   CO187827   CO189633   CO195373   CO271371   CO280914   CO293318   CO300657   CO300970   CO302732   CO306183   CO316290   CO321555   CO327002   CO339653   CZ488207   EC064974   EC072159   EC078214   EC195195   EC197880   EC203513   EC210756   EC210903   EC211338   EC211801   EC212558   EC220424   EC227168   EC229859   EC246092   EC257893
UniProtKB/Swiss-Prot
UniProtKB/TrEMBL	Q6AWJ8 Q95V09 Q9NHE9 A1Z9D7
Maps to
Does NOT map to
Identified with	CK00539 (Kopczynski et al., 1998) LD10754 LP19005 (BDGP Project Members, 2000-)
Mapped Features & Mutations
Please see GBrowse or insertion reports for information on insertions of transgenic constructs and features not listed here Type Symbol & Location Additional Notes References
External Data
Linkouts	DEDB - Drosophila exon database: splicing graphs • 12628
Crossreferences
Expression Data
FlyBase-Curated Data
Transcript and Protein data	Please see the FlyBase Gene Expression Report for details of gene expression from the literature.
Summary of Transcript Expression
Stage Tissue/Position Reference
Marker for
Subcellular Localization
CV Term
Summary of Polypeptide Expression
Stage Tissue/Position Reference
Marker for
Subcellular Localization
CV Term
External Data & Images
Linkouts	FLIGHT - Cell culture data for RNAi and other high-throughput technologies • FBgn0000119 FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array • FBgn0000119 GEO (NCBI) - Gene expression data: microarray and other high-throughput technologies • FBgn0000119 BDGP expression data • Show all BDGP original images, annotations and embryonic microarray data for arr FlyExpress - Embryonic expression images (BDGP data) • Show all FlyExpress processed images for arr Stage(s) 1-3 Stage(s) 4-6 Stage(s) 9-10 Stage(s) 11-12 Stage(s) 13-16
Alleles & Phenotypes
Summary of Allele Phenotypes
Lethality Allele lethal | recessive arrk08131 lethal | embryonic stage arr1 lethal | embryonic stage | recessive arr12-1 arr16-1 arr4 arrL1 arrunspecified lethal, with Scer\GAL4GMR.PF, Scer\GAL4ey.PH arrGD121 arrGD14404 arrGD2617 lethal, with Scer\GAL4Act5C arrGD121 arrGD14404 arrGD2617 cell lethal | partially | recessive | somatic clone arrunspecified Other Phenotypes Allele visible | recessive | somatic clone arr2 visible, with Scer\GAL4Bx-MS1096 arrScer\UAS.cWb cell death increase | somatic clone arr2 cell growth defective | somatic clone arr2 tissue polarity defective | cell non-autonomous | recessive | somatic clone arr2 arrG6 tissue polarity defective | recessive | somatic clone arrG68 Phenotype manifest in Allele denticle belt arr1 arrdsRNA.cTa midgut constriction 2 arr12-1 arr16-1 arr4 arrL1 gastric caecum arr12-1 arr16-1 arr4 arrL1 embryonic central nervous system arr12-1 arr16-1 arr4 arrL1 embryonic peripheral nervous system arr12-1 arr16-1 arr4 arrL1 eye arr12-1 arr16-1 arr4 arrL1 head arr12-1 arr16-1 arr4 arrL1 cuticle arr12-1 arr16-1 arr4 arrL1 wing | somatic clone arr2 wing margin bristle | somatic clone arr2 ommatidium | ectopic | somatic clone arr2 interommatidial bristle | somatic clone arr2 retina | ectopic arr2 ommatidium | somatic clone arr2 arrG6 arrG68 eye | somatic clone arr2 arr63D retina | somatic clone arr2 dorsal mesothoracic disc | somatic clone arr2 secondary pigment cell | somatic clone arr2 tertiary pigment cell | somatic clone arr2 photoreceptor cell | somatic clone arr63D eye disc | somatic clone arr63D macrochaeta arrL50A wing, with Scer\GAL4Bx-MS1096 arrScer\UAS.cWb wing sensillum | ectopic, with Scer\GAL4Bx-MS1096 arrScer\UAS.cWb wing, with Scer\GAL4en-e16E arrdsRNA.Scer\UAS embryonic/first instar larval cuticle arrdsRNA.cTa arrunspecified denticle arrunspecified embryonic epidermis arrunspecified
Classical Alleles ( 15 )
For All Classical Alleles Show
Allele of arr Class Mutagen Stocks Known lesion arr12-1 loss of function ethyl methanesulfonate 0 -- arr134 P-element activity 0 Yes arr16-1 ethyl methanesulfonate 0 Yes arr1 ethyl methanesulfonate 0 -- arr2 ethyl methanesulfonate 2 Yes arr4 loss of function ethyl methanesulfonate 0 -- arr63D ethyl methanesulfonate 0 -- arrEY04453 P-element activity 1 -- arrG68 0 -- arrG6 0 -- arrL1 ethyl methanesulfonate 0 Yes arrL50A P-element activity 0 Yes arrf05606 piggyBac transposase 1 -- arrk08131 P-element activity 2 -- arrunspecified 0 --
Alleles Carried on Transgenic Constructs ( 22 )
For All Alleles Carried on Transgenic Constructs Show
Allele of arr Class Mutagen Stocks Known lesion arr1185-1393 in vitro construct | RNAi 0 Yes arrAct5C.T:Ivir\HA1 in vitro construct | regulatory fusion 0 Yes arrC.Scer\UAS.T:Myr1,T:Ivir\HA1 in vitro construct | regulatory fusion in vitro construct | coding region fusion 0 Yes arrGD121 in vitro construct | RNAi in vitro construct | regulatory fusion 2 Yes arrGD14404 in vitro construct | RNAi in vitro construct | regulatory fusion 1 Yes arrGD2617 in vitro construct | RNAi in vitro construct | regulatory fusion 2 Yes arrIntra.T:Myr1,T:Zzzz\FLAG in vitro construct | coding region fusion in vitro construct | regulatory fusion 0 Yes arrScer\FRT.Scer\UAS.T:Ivir\HA1 in vitro construct | regulatory fusion in vitro construct | coding region fusion 0 Yes arrScer\FRT.Scer\UAS in vitro construct | regulatory fusion 0 Yes arrScer\UAS.T:Ivir\HA1 in vitro construct | regulatory fusion 0 -- arrScer\UAS.T:wg,T:Avic\GFP,T:Hsap\MYC in vitro construct | regulatory fusion in vitro construct | coding region fusion 0 Yes arrScer\UAS.cPa.T:Ivir\HA1 in vitro construct | regulatory fusion in vitro construct | coding region fusion 0 Yes arrScer\UAS.cRa.T:Ivir\HA1 FLPase 0 Yes arrScer\UAS.cWa in vitro construct | regulatory fusion 0 Yes arrScer\UAS.cWb FLPase 0 Yes arra.1185-1393 in vitro construct | RNAi 0 Yes arrdsRNA.Scer\FRT.Scer\UAS in vitro construct | regulatory fusion 0 Yes arrdsRNA.Scer\UAS FLPase in vitro construct | RNAi 0 Yes arrdsRNA.cGa in vitro construct | RNAi 0 Yes arrdsRNA.cMa in vitro construct | RNAi 0 -- arrdsRNA.cTa in vitro construct | RNAi 0 Yes arrΔC.Scer\UAS.T:Ivir\HA1 in vitro construct | regulatory fusion in vitro construct | coding region fusion in vitro construct | deletion 0 Yes
Aneuploid Aberrations
Useful deficiency	Df(2R)CX1
Useful duplication	Dp(2;2)SMG45
Disrupted in	Df(2R)8-104 (Vaizel-Ohayon and Schejter, 1999) Df(2R)CX1 (Baker, 2005.1.6, BDGP Project Members, 1994-1999)
Not disrupted in	Df(2R)vg-B (BDGP Project Members, 1994-1999)
Transgenic Constructs & Insertions
Transgenic Constructs
	Type of construct Name Expression data UAS construct P{GD121} NA P{GD14404} NA P{GD2617} NA P{UAS(-FRT)arr.HA} NA P{UAS(FRT.T:Hcri\RFP)Rab7.dsRNA} NA P{UAS(FRT.w+)arr.HA} NA P{UAS-arr.FRT.Wb} NA P{UAS-arr.GFP} NA P{UAS-arr.HA} NA P{UAS-arr.Wa} NA P{UAS-arr.Wb} NA P{UAS-arr.flu} NA P{UAS-arr.ΔC.HA} NA characterization construct P{UAS-arr.intra.myr.FALFG} NA P{UAS-fz2:arr.intra.MYC} NA
Insertions
	Type of insertions Name Expression data miscellaneous insertions PBac{WH}arrf05606 NA P{?unk}arr134 NA insertion of enhancer trap P{lArB}arrL50A No P{lacW}arrk08131 No insertion of mobile activating element P{EPgy2}arrEY04453 NA
Related Comments
	Please look at the allele reports for the complete phenotype data Mutant alleles are embryonic lethal with extra denticle bands, especially on ventral midline.   Deficiency homozygotes for arr fail to complete embryogenesis: the second midgut constriction and the gastric caecae are missing, and axonal extension also shows defects. (Li et al., 1995) Mutations in arr disrupt midgut morphogenesis and nervous system development. Failure of somatic arr- clones to grow to normal size strongly suggests that arr is essential for cell viability or proliferation or both. Germline clonal analysis reveals arr is required for germline proliferation and oocyte development. (Li and Kaufman, 1996)
Gene Ontology: Function, Process & Cellular Component ( 10 )
Molecular Function
CV term Evidence References
Wnt receptor activity inferred from direct assay (Rives et al., 2006)
low-density lipoprotein receptor activity inferred from sequence or structural similarity with MGD:Lrp5; MGI:MGI:1278315 AND inferred from sequence or structural similarity with MGD:Lrp6; MGI:MGI:1298218 (Wehrli et al., 2000) traceable author statement (Wehrli, 2000.10.22)
protein binding inferred from direct assay (Piddini et al., 2005)
Biological Process
CV term Evidence References
Wnt receptor signaling pathway inferred from mutant phenotype (Wehrli et al., 2000) traceable author statement (Wehrli, 2000.10.22)
compound eye morphogenesis inferred from mutant phenotype (Tomlinson, 2003)
protein targeting to lysosome inferred from mutant phenotype (Piddini et al., 2005)
regulation of compound eye pigmentation inferred from mutant phenotype (Lim and Tomlinson, 2006)
Cellular Component
CV term Evidence References
colocalizes_with early endosome inferred from direct assay (Rives et al., 2006)
colocalizes_with late endosome inferred from direct assay (Rives et al., 2006)
plasma membrane inferred from electronic annotation (Roth and Foulger, 2002.4.24) inferred from direct assay (Rives et al., 2006)
Sequence Ontology: Class of Gene
	protein_coding_gene   nuclear_gene
Interactions & Pathways
Summary of Genetic Interactions
Interacts with	Please look at the allele data for full details of the genetic interactions arr allele Gene References arr2 wg (Wehrli and Tomlinson, 1998) arr::fz2 (Tolwinski et al., 2003) arrdsRNA.cTa Axn (Tolwinski et al., 2003) arrunspecified dsh (Wehrli et al., 2000)
External Data
Linkouts	BioGRID - Interaction data, including yeast 2-hybrid and genetic interactions • 68943
Orthologs
Genome-wide drosophilid orthologs	Dana\GF11183 Dere\GG22489 Dgri\GH23152 Dmoj\GI21337 Dper\GL17073 Dpse\GA19223 Dsec\GM20276 Dvir\GJ19682 Dwil\GK15922 Dyak\GE13360
Curated drosophilid orthologs	Dpse\GA19223
Linkouts	InParanoid orthologs - Eukaryotic orthologs • ENSANGP00000020132 Anopheles gambiae (Ensembl) ENSBTAP00000007756 Bos taurus (Ensembl) ENSCAFP00000019674 Canis familiaris (Ensembl) ENSCINP00000017834 Ciona intestinalis (Ensembl) NEWSINFRUP00000180385 Takifugu rubripes (Ensembl) ENSGALP00000011315 Gallus gallus (Ensembl) ENSP00000261349 Homo sapiens (Ensembl) ENSMMUP00000030693 Macaca mulatta (Ensembl) ENSMODP00000022743 Monodelphis domestica (Ensembl) ENSPTRP00000008035 Pan troglodytes (Ensembl) ENSRNOP00000008486 Rattus norvegicus (Ensembl) GSTENP00005065001 Tetraodon nigroviridis (Ensembl) ENSXETP00000022101 Xenopus tropicalis (Ensembl) MGI:1298218 Mus musculus (Informatics)
Functional Complementation between Species
Inter-Species Misexpression Data
Produces phenotype in
Produces NO phenotype in
Stocks & Reagents
Stocks Listed in FlyBase ( 11 )
Harvard	- PBac{WH}arrf05606
VDRC	v6707 w1118; P{GD121}v6707