Gene Dmel\Act87E

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General Information

Symbol

Dmel\Act87E

Species

D. melanogaster

Name

Actin 87E

Annotation symbol

CG18290

Feature type

protein_coding_gene

FlyBase ID

FBgn0000046

Created / Updated

2003-12-01/2003-12-01

Genomic Location

Chromosome (arm)

Recombination map

3-[53]

Cytogenetic map

87E11-87E11

Sequence location

3R:9,251,707..9,253,810 [+]

Map ( GBrowse )

Summary Information

Automatically generated summary

See sections below for more information

The gene Actin 87E is referred to in FlyBase by the symbol Act87E (CG18290, FBgn0000046). It has the cytological map location 87E11. Its sequence location is 3R:9251707..9253810. Its molecular function is described as: structural constituent of cytoskeleton; ATP binding; protein binding. It is involved in the biological process cytoskeleton organization and biogenesis. 2 alleles are reported. No phenotypic data is available. It has 2 annotated transcripts and 2 annotated polypeptides.

Phenotypic Description from the Red Book (Lindsley & Zimm 1992)

Gene/Allele symbols may differ from current usage

Act87E

Encodes actin found in larval muscle and adult cephalic and abdominal muscle (Fyrberg, Mahaffey, Bond, and Davidson, 1983, Cell 33: 115-23).

Detailed Mapping Data

FlyBase Computed Cytological Location

Cytogenetic map

Evidence for location

87E11-87E11

Limits computationally determined from genome sequence between P{PZ}CtBP⁰³⁴⁶³ and P{lacW}B52^s2249&P{lacW}flfl^L4179

Experimentally Determined Cytological Location

Cytogenetic map

Notes

References

87E-87E

(determined by in situ hybridisation)

(Drosopoulou et al., 1997)

87E9-87E12

(determined by in situ hybridisation)

(Ranz et al., 1997)

Experimentally Determined Recombination Data

Location

3-[53]

Left of (cM)

Right of (cM)

Notes

Molecular Map Data

Gene Order (in direction of increasing cytology)

References

Gene Order (overall orientation not stated)

References

Gene Model & Products

Please see the GBrowse view of Dmel\Act87E for information on other features

Comments on Gene Model

Transcript Data

Annotated Transcripts

Name

FlyBase ID

RefSeq ID

Length (nt)

Associated CDS (aa)

Act87E-RA

FBtr0082785

NM_057743

1564

376

Act87E-RB

FBtr0082786

NM_169525

1642

376

Additional Transcript Data & Comments

Reported size (kB)

1.7 (northern blot)

(Manseau et al., 1988)

1.65 (northern blot)

(Tobin et al., 1990)

Comments

External Data

Crossreferences

Polypeptide Data

Annotated Polypeptides

Name

FlyBase ID

Predicted MW (kD)

Length (aa)

Theoretical pI

RefSeq ID

GenBank protein

Act87E-PA

FBpp0082253

41.8

376

5.15

NP_477091

AAF54950

Act87E-PB

FBpp0082254

41.8

376

5.15

NP_731812

AAN13567

Additional Polypeptide Data & Comments

Reported size (kD)

376 (aa)

(Manseau et al., 1988)

Comments

External Data

Linkouts

PANTHER - Protein classification by function, families, and pathways

•

FBgn0000046

Crossreferences

InterPro domains - A database of protein families, domains, and functional sites

•

Actin/actin-like (IPR004000)

Actin, conserved site (IPR004001)

Sequences Consistent with the Gene Model

DDBJ /
EMBL /
GenBank

DNA sequence

Protein sequence

Name

P10981

UniProtKB/TrEMBL

Maps to

(FlyBase, 1992-)

Does NOT map to

84H8

(Deak et al., 1997)

Identified with

(BDGP Project Members, 2000-)

D1202

(BDGP Project Members, 1994-1999)

Mapped Features & Mutations

Please see GBrowse or insertion reports for information on insertions of transgenic constructs and features not listed here

Type

Symbol & Location

Additional Notes

References

External Data

Linkouts

Crossreferences

EPD - Eukarytoic Promoter Database, an annotated collection of POL II promoters

•

23022

Expression Data

FlyBase-Curated Data

Transcript and
Protein data

Please see the FlyBase Gene Expression Report for details of gene expression from the literature.

Summary of Transcript Expression

Stage

Tissue/Position

Reference

larval stage

larval muscle system

(Tobin et al., 1990)

Marker for

Subcellular Localization

CV Term

Summary of Polypeptide Expression

Stage

Tissue/Position

Reference

Marker for

Subcellular Localization

CV Term

External Data & Images

Linkouts

FLIGHT - Cell culture data for RNAi and other high-throughput technologies

•

FBgn0000046

FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array

•

FBgn0000046

GEO (NCBI) - Gene expression data: microarray and other high-throughput technologies

•

FBgn0000046

BDGP expression data

•

Show all BDGP original images, annotations and embryonic microarray data for Act87E

FlyExpress - Embryonic expression images (BDGP data)

•

Show all FlyExpress processed images for Act87E

Alleles & Phenotypes

Summary of Allele Phenotypes

Phenotype manifest in

Allele

Classical Alleles ( 0 )

For All Classical Alleles Show

Allele of Act87E	Class	Mutagen	Stocks	Known lesion

Alleles Carried on Transgenic Constructs ( 2 )

For All Alleles Carried on Transgenic Constructs Show

Allele of Act87E	Class	Mutagen	Stocks	Known lesion
Act87E^GD9553		in vitro construct \| RNAi in vitro construct \| regulatory fusion	0	Yes
Act87E^{Scer\UAS.P\T.T:Avic\GFP}		in vitro construct \| regulatory fusion in vitro construct \| coding region fusion	2	Yes

Aneuploid Aberrations

Useful deficiency

Df(3R)ry619

Disrupted in

Df(3R)Exel7320

(Ryder, 2004.4.26)

Df(3R)l26c

(Manseau et al., 1988)

Df(3R)ry619

(Manseau et al., 1988)

Not disrupted in

Df(3R)lC4a

(Manseau et al., 1988)

Df(3R)ry1168

(Manseau et al., 1988)

Transgenic Constructs & Insertions

Transgenic Constructs

Type of construct

Name

Expression data

UAS construct

P{GD9553}

P{UASp-GFP.Act87E}

Insertions

Type of insertions

Name

Expression data

Related Comments

Please look at the allele reports for the complete phenotype data

No lethal mutations in Act87E gene were isolated during a molecular and genetic analysis of region including Act87E gene. Possible explanations include non-lethality of Act87E mutations or substitution of Act87E function by other actin-encoding genes in the genome.

(Manseau et al., 1988)

Translation of actin RNA in early embryos injected with initiation factors has been studied.

(Hongo and Jacobs-Lorena, 1991)

Gene Ontology: Function, Process & Cellular Component ( 6 )

Molecular Function

CV term

Evidence

References

ATP binding

inferred from electronic annotation with InterPro:IPR004001

(FlyBase Curators et al., 2004-)

protein binding

inferred from electronic annotation with InterPro:IPR004000

(FlyBase Curators et al., 2004-)

structural constituent of cytoskeleton

inferred from sequence or structural similarity

(Goldstein and Gunawardena, 2000)

Biological Process

CV term

Evidence

References

cytoskeleton organization and biogenesis

inferred from sequence or structural similarity

(Goldstein and Gunawardena, 2000)

Cellular Component

CV term

Evidence

References

actin filament

inferred from sequence or structural similarity

(Goldstein and Gunawardena, 2000)

non-traceable author statement

(Swiss-Prot Project Members, 1989.7.1)

lipid particle

inferred from direct assay

(Cermelli et al., 2006)

Sequence Ontology: Class of Gene

protein_coding_gene

nuclear_gene

Interactions & Pathways

Summary of Genetic Interactions

Interacts with

Please look at the allele data for full details of the genetic interactions

Act87E allele

Gene

References

External Data

Linkouts

BioGRID - Interaction data, including yeast 2-hybrid and genetic interactions

•

71541

Orthologs

Genome-wide drosophilid orthologs

Dana\GF17602

Dere\GG19688

Dgri\GH19045

Dmoj\GI24339

Dpse\GA14877

Dsec\GM24109

Dvir\ActE1

Dwil\GK13943

Dyak\GE26273

Curated drosophilid orthologs

Dbuz\Act87E

Dpse\GA14877

Drep\Act87E

Dvir\ActE1

Linkouts

InParanoid orthologs - Eukaryotic orthologs

•

ENSANGP00000016398
Anopheles gambiae (Ensembl)
ZDB-CDNA-041010-78
Danio rerio (Zfin)
ZDB-GENE-041010-78
Danio rerio (Zfin)
SPBC32H8.12c
Schizosaccharomyces pombe (Sanger)

Functional Complementation between Species

Inter-Species Misexpression Data

Produces phenotype in

Produces NO phenotype in

Stocks & Reagents

Stocks Listed in FlyBase ( 2 )

Bloomington

9249

w^*; P{UASp-GFP.Act87E}7-6

9250

P{UASp-GFP.Act87E}12-1, w^*

Genomic Clones ( 1 )

BACR06H18

cDNA Clones ( 58 )

Please Note

This section lists cDNAs and ESTs that fall within the genomic extent of the gene model, which may include cDNAs and ESTs of genes within introns, or of overlapping genes. Please see GBrowse for alignment of the cDNAs and ESTs to the gene model.

cDNA Clones, Fully Sequenced

BDGP DGC clones

Other clones

cDNA Clones, End Sequenced (ESTs)

BDGP DGC clones

Other clones

RNAi & Array Information

Affy Oligo

Linkouts

DRSC - RNAi screening (Harvard): high-throughput cell culture data and design

•

FBgn0000046

GenomeRNAi - RNAi phenotypes (Heidelberg): high-throughput cell culture data

•

FBgn0000046

Antibody Information

Other Information

Discoverer

Etymology

Identification

Position Effect Variegation Data

Relationship to Other Genes

Source for database identity of

Source for database merge of

Source for merge of: Act87E CG18290

(Gelbart, 2000.4.13)

Additional comments

Comments About Role

Encodes actin found in larval muscle and adult cephalic and abdominal muscle (FBrf0038990).

Comments About Molecular Function

ChEST reveals this is a target of Mef2.

(Junion et al., 2005)

Other Comments

Sequence analysis demonstrates insect muscle actins form a distinct family of closely related proteins significantly diverged from cytoplasmic actins.

(Mounier et al., 1992)

Age related changes in Act87E expression have been studied by Northern analysis.

(Labuhn and Brack, 1997)

In a sample of 79 genes with multiple introns, 33 showed significant heterogeneity in G+C content among introns of the same gene and significant positive correspondence between the intron and the third codon position G+C content within genes. These results are consistent with selection adding against preferred codons at the start of genes.

(Kliman and Eyre-Walker, 1998)

External Crossreferences & Linkouts

Sequence Crossreferences

RefSeq (Transcripts)

•

RefSeq (Proteins)

•

DDBJ / EMBL / GenBank

•