Gene Dmel\Act79B

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General Information

Symbol

Dmel\Act79B

Species

D. melanogaster

Name

Actin 79B

Annotation symbol

CG7478

Feature type

protein_coding_gene

FlyBase ID

FBgn0000045

Created / Updated

2003-12-01/2003-12-01

Genomic Location

Chromosome (arm)

Recombination map

Cytogenetic map

79A6-79A6

Sequence location

3L:21,978,331..21,980,290 [+]

Map ( GBrowse )

Summary Information

Automatically generated summary

See sections below for more information

The gene Actin 79B is referred to in FlyBase by the symbol Act79B (CG7478, FBgn0000045). It has the cytological map location 79A6. Its sequence location is 3L:21978331..21980290. Its molecular function is described as: structural constituent of cytoskeleton; ATP binding; protein binding. It is involved in the biological process cytoskeleton organization and biogenesis. 4 alleles are reported. No phenotypic data is available. It has one annotated transcript and one annotated polypeptide.

Phenotypic Description from the Red Book (Lindsley & Zimm 1992)

Gene/Allele symbols may differ from current usage

Act79B

According to Zulauf et al. (1981), Act79B appears to code for actin I, a larval muscle-specific actin. Initial translation product, which migrates as actin II, apparently acetylated to become actin I. Using probe from 3' transcribed-but-not-translated sequences, S'nchez et al. demonstrated two minor peaks of transcription during embryogenesis and major peaks during first and second instar and to a lesser degree in the pupa. Fyrberg, Mahaffy, Bond, and Davidson, (1983, Cell 33: 115-23) on the other hand, find Act79B to be expressed in adult legs and tubular muscles of thorax, including direct flight muscles, pleurosternal muscles, and muscles of various leg segments. In addition, Act79B is expressed in muscles that support the head and abdomen, in the scutellar pulsatile organ, and in two pairs of abdominal muscles that are present only in male flies (Courchesne-Smith, and Tobin, 1989, Dev. Biol. 133: 313-21). One of three actin genes expressed in wing development each with its characteristic developmental profile; peak activity at 80h of pupal age (Petersen, Bond, Mitchell, and Davidson, 1985, Dev. Genet. 5: 219-25.

Detailed Mapping Data

FlyBase Computed Cytological Location

Cytogenetic map

Evidence for location

79A6-79A6

Limits computationally determined from genome sequence between P{lacW}l(3)j1B10^j1B10 and P{lacW}l(3)j2C4^j2C4

Experimentally Determined Cytological Location

Cytogenetic map

Notes

References

79B-79B

(determined by in situ hybridisation)

(Drosopoulou et al., 1997)

79B-79B

(determined by in situ hybridisation)

(Zulauf et al., 1981)

79B-79B

(determined by in situ hybridisation)

(Fyrberg et al., 1981)

Experimentally Determined Recombination Data

Location

Left of (cM)

Right of (cM)

Notes

Molecular Map Data

Gene Order (in direction of increasing cytology)

References

Gene Order (overall orientation not stated)

References

Gene Model & Products

Please see the GBrowse view of Dmel\Act79B for information on other features

Comments on Gene Model

Transcript Data

Annotated Transcripts

Name

FlyBase ID

RefSeq ID

Length (nt)

Associated CDS (aa)

Act79B-RA

FBtr0078478

NM_079486

1522

376

Additional Transcript Data & Comments

Reported size (kB)

Comments

External Data

Crossreferences

Polypeptide Data

Annotated Polypeptides

Name

FlyBase ID

Predicted MW (kD)

Length (aa)

Theoretical pI

RefSeq ID

GenBank protein

Act79B-PA

FBpp0078131

41.8

376

5.16

NP_524210

AAF51800

Additional Polypeptide Data & Comments

Reported size (kD)

Comments

External Data

Linkouts

PANTHER - Protein classification by function, families, and pathways

•

FBgn0000045

Crossreferences

InterPro domains - A database of protein families, domains, and functional sites

•

Actin/actin-like (IPR004000)

Actin, conserved site (IPR004001)

Sequences Consistent with the Gene Model

DDBJ /
EMBL /
GenBank

DNA sequence

Protein sequence

Name

P02574

UniProtKB/TrEMBL

Maps to

Does NOT map to

Identified with

GH04529

(BDGP Project Members, 2000-)

D1373

(BDGP Project Members, 1994-1999)

Mapped Features & Mutations

Please see GBrowse or insertion reports for information on insertions of transgenic constructs and features not listed here

Type

Symbol & Location

Additional Notes

References

External Data

Linkouts

DEDB - Drosophila exon database: splicing graphs

•

6106

Crossreferences

Expression Data

FlyBase-Curated Data

Transcript and
Protein data

Please see the FlyBase Gene Expression Report for details of gene expression from the literature.

Summary of Transcript Expression

Stage

Tissue/Position

Reference

Marker for

Subcellular Localization

CV Term

Summary of Polypeptide Expression

Stage

Tissue/Position

Reference

Marker for

Subcellular Localization

CV Term

External Data & Images

Linkouts

FLIGHT - Cell culture data for RNAi and other high-throughput technologies

•

FBgn0000045

FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array

•

FBgn0000045

GEO (NCBI) - Gene expression data: microarray and other high-throughput technologies

•

FBgn0000045

Alleles & Phenotypes

Summary of Allele Phenotypes

Lethality

Allele

viable

Act79B^f00364

Sterility

Allele

fertile

Act79B^f00364

Phenotype manifest in

Allele

Classical Alleles ( 1 )

For All Classical Alleles Show

Allele of Act79B	Class	Mutagen	Stocks	Known lesion
Act79B^f00364		piggyBac transposase	2	--

Alleles Carried on Transgenic Constructs ( 3 )

For All Alleles Carried on Transgenic Constructs Show

Allele of Act79B	Mutagen	Stocks	Known lesion
Act79B^GD1352	in vitro construct \| RNAi in vitro construct \| regulatory fusion	1	Yes
Act79B^{R291H.Scer\UAS.P\T.T:Avic\GFP}	in vitro construct \| site directed mutagenesis	2	Yes
Act79B^{Scer\UAS.P\T.T:Avic\GFP}	in vitro construct \| regulatory fusion in vitro construct \| coding region fusion	2	Yes

Aneuploid Aberrations

Useful deficiency

Df(3L)78E2-3;79E

Transgenic Constructs & Insertions

Transgenic Constructs

Type of construct

Name

Expression data

characterization construct

P{Act79B::Act88F^cFa}

P{UASp-GFP.Act79B::Act88F.79B.C-T.88F.N-T}

P{UASp-GFP.Act79B::Act88F.79B.N-T.88F.C-T}

reporter construct

P{Act79B-lacZ.CS}

Yes

P{Act79B-lacZ.K}

UAS construct

P{GD1352}

P{UASp-GFP.Act79B.R291H}

P{UASp-GFP.Act79B}

Insertions

Type of insertions

Name

Expression data

miscellaneous insertions

PBac{WH}Act79B^f00364

Related Comments

Please look at the allele reports for the complete phenotype data

Translation of actin RNA in early embryos injected with initiation factors has been studied.

(Hongo and Jacobs-Lorena, 1991)

Gene Ontology: Function, Process & Cellular Component ( 5 )

Molecular Function

CV term

Evidence

References

ATP binding

inferred from electronic annotation with InterPro:IPR004001

(FlyBase Curators et al., 2004-)

protein binding

inferred from electronic annotation with InterPro:IPR004000

(FlyBase Curators et al., 2004-)

structural constituent of cytoskeleton

inferred from sequence or structural similarity

(Goldstein and Gunawardena, 2000)

inferred from sequence or structural similarity

(FlyBase, 1992-)

Biological Process

CV term

Evidence

References

cytoskeleton organization and biogenesis

inferred from sequence or structural similarity

(Goldstein and Gunawardena, 2000)

Cellular Component

CV term

Evidence

References

actin filament

inferred from sequence or structural similarity

(Goldstein and Gunawardena, 2000)

non-traceable author statement

(Swiss-Prot Project Members, 1986.7.21)

Sequence Ontology: Class of Gene

protein_coding_gene

nuclear_gene

Interactions & Pathways

Summary of Genetic Interactions

Interacts with

Please look at the allele data for full details of the genetic interactions

Act79B allele

Gene

References

External Data

Linkouts

BioGRID - Interaction data, including yeast 2-hybrid and genetic interactions

•

65684

Orthologs

Genome-wide drosophilid orthologs

Dana\GF10940

Dere\GG16241

Dgri\GH14437

Dmoj\GI13569

Dpse\GA20380

Dsec\GM22426

Dvir\ActD1

Dwil\GK10683

Curated drosophilid orthologs

Dbuz\Act79B

(Laayouni et al., 2000)

Dpse\GA20380

Drep\Act79B

Dvir\ActD1

Linkouts

Functional Complementation between Species

Inter-Species Misexpression Data

Produces phenotype in

Produces NO phenotype in

Stocks & Reagents

Stocks Listed in FlyBase ( 7 )

Harvard

PBac{WH}Act79B^f00364

VDRC

v9776

w¹¹¹⁸; P{GD1352}v9776/TM3

Bloomington

9247

w^*; P{UASp-GFP.Act79B}3-1

9248

w^*; P{UASp-GFP.Act79B}2-2

9246

w^*; P{UASp-GFP.Act79B.R291H}2-2

9245

P{UASp-GFP.Act79B.R291H}30-1, w^*

18320

w¹¹¹⁸; PBac{WH}Act79B^f00364

Genomic Clones ( 1 )

BACR17D14

cDNA Clones ( 12 )

cDNA Clones, Fully Sequenced

BDGP DGC clones

GH04529

Other clones

cDNA Clones, End Sequenced (ESTs)

BDGP DGC clones

Other clones

RNAi & Array Information

Affy Oligo

Linkouts

DRSC - RNAi screening (Harvard): high-throughput cell culture data and design

•

FBgn0000045

GenomeRNAi - RNAi phenotypes (Heidelberg): high-throughput cell culture data

•

FBgn0000045

Antibody Information

Other Information

Discoverer

Etymology

Identification

Position Effect Variegation Data

Relationship to Other Genes

Source for database identity of

Source for database merge of

Additional comments

The Act42A gene may be derived from the Act79B gene by retroposition.

(Betran et al., 2002)

Comments About Role

Encodes a tubular muscle actin expressed in adult legs and tubular muscles of thorax, including direct flight muscles, pleurosternal muscles and muscles of various leg segments (FBrf0038990). In addition, Act79B is expressed in muscles that support the head and abdomen, in the scutellar pulsatile organ and in two pairs of abdominal muscles that are present only in male flies (FBrf0049489). One of three actin genes expressed in wing development each with its characteristic developmental profile; peak activity at 80h of pupal age (FBrf0042391).

Comments About Molecular Function

Other Comments

Sequence analysis demonstrates insect muscle actins form a distinct family of closely related proteins significantly diverged from cytoplasmic actins.

(Mounier et al., 1992)

Age related changes in Act79B expression have been studied by Northern analysis.

(Labuhn and Brack, 1997)

There are differences in the level of Act79B gene expression among the various abdominal muscles of both sexes, abdominal expression is not limited primarily to male sex structures.

(Ohshima et al., 1997)

In a sample of 79 genes with multiple introns, 33 showed significant heterogeneity in G+C content among introns of the same gene and significant positive correspondence between the intron and the third codon position G+C content within genes. These results are consistent with selection adding against preferred codons at the start of genes.

(Kliman and Eyre-Walker, 1998)

External Crossreferences & Linkouts

Sequence Crossreferences

RefSeq (Transcripts)

•

NM_079486

RefSeq (Proteins)

•

NP_524210

DDBJ / EMBL / GenBank

•

UniProtKB/Swiss-Prot

•

P02574

Other Crossreferences

InterPro domains - A database of protein families, domains, and functional sites

•

Actin/actin-like (IPR004000)

Actin, conserved site (IPR004001)

Linkouts

BioGRID - Interaction data, including yeast 2-hybrid and genetic interactions

•

65684

DEDB - Drosophila exon database: splicing graphs

•

6106

DRSC - RNAi screening (Harvard): high-throughput cell culture data and design

•

FBgn0000045

FLIGHT - Cell culture data for RNAi and other high-throughput technologies

•

FBgn0000045

FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array

•

FBgn0000045

FlyMine - Integrated genomics database for Drosophila, Anopheles, and C.elegans

•

FBgn0000045

GEO (NCBI) - Gene expression data: microarray and other high-throughput technologies

•

FBgn0000045

GenomeRNAi - RNAi phenotypes (Heidelberg): high-throughput cell culture data

•

FBgn0000045

PANTHER - Protein classification by function, families, and pathways

•

FBgn0000045

Synonyms & Secondary IDs ( 11 )

Reported As

Symbol Synonym

143060_f_at

(Kristensen et al., 2005)

act 79B

(Labuhn and Brack, 1997)

act79B

(He and Haymer, 1995)

Act79B

(Stuart et al., 2007, Montana and Littleton, 2006)

Actin

(Balasubramanian et al., 2004, Eberharter and Becker, 2004, Jones, 1999, Hongo and Jacobs-Lorena, 1991)

actin

(Xia and Karin, 2004, Kwon and Scholey, 2004, Strickland and Burgess, 2004, Delmas et al., 2004, Miller, 2003, Muller and Littlewood-Evans, 2001, Adams et al., 2000, Jacinto and Baum, 2003, Baum, 2002, Biggin, 2001, Noselli and Agnes, 1999, OOta and Saitou, 1999, Brault et al., 1999, Bearer, 1991, Padash Barmchi et al., 2005)

CG7478

(Goldstein and Gunawardena, 2000, )

(Bondinas et al., 2002, Bondinas et al., 2001)

mus79B

(White and Crother, 1999)

Name Synonym

79B Actin

(Roy and VijayRaghavan, 1999)

actin

(Bosch et al., 2005, Costantino et al., 2007, Lo, 2006, Harris and Peifer, 2004, Zeitler et al., 2004, Cytrynbaum et al., 2005, Sordella and Van, 2006)

Actin

(Mulinari et al., 2007, Griemert et al., 2007)

Actin 79B

Secondary FlyBase IDs

References ( 91 )

Generate a list of

All references relating to this gene ( 91 )

List References by type

Recent research papers ( 2 )

Stuart et al., 2007, Nature 445(7123): 95--101: A systems biology analysis of the Drosophila phagosome. [FBrf0195342]
Montana and Littleton, 2006, J. Biol. Chem. 281(12): 8100--8109: Expression profiling of a hypercontraction-induced myopathy in Drosophila suggests a compensatory cytoskeletal remodeling response. [FBrf0190896]

Show all research papers ...

Recent reviews ( 2 )

Lo, 2006, Dev. Biol. 294(2): 280--291: [FBrf0198609]
Sordella and Van, 2006, Cell 127(1): 37--39: Driving actin dynamics under the influence of alcohol. [FBrf0192697]

Show all reviews ...