Gene Dmel\Act57B

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General Information

Symbol

Dmel\Act57B

Species

D. melanogaster

Name

Actin 57B

Annotation symbol

CG10067

Feature type

protein_coding_gene

FlyBase ID

FBgn0000044

Created / Updated

2005-09-09/2005-09-09

Genomic Location

Chromosome (arm)

Recombination map

Cytogenetic map

57B5-57B5

Sequence location

2R:16,831,533..16,833,945 [+]

Map ( GBrowse )

Summary Information

Automatically generated summary

See sections below for more information

The gene Actin 57B is referred to in FlyBase by the symbol Act57B (CG10067, FBgn0000044). It has the cytological map location 57B5. Its sequence location is 2R:16831533..16833945. Its molecular function is described as: structural constituent of cytoskeleton; glucuronosyltransferase activity; ATP binding; protein binding. It is involved in the biological processes: cytoskeleton organization and biogenesis; cytokinesis; heart development; chondroitin sulfate biosynthetic process; heparan sulfate proteoglycan biosynthetic process. 5 alleles are reported. The phenotype of these alleles is annotated with germline cyst. It has 2 annotated transcripts and 2 annotated polypeptides.

Phenotypic Description from the Red Book (Lindsley & Zimm 1992)

Gene/Allele symbols may differ from current usage

Act57A

According to Fyrberg and Davidson, Act57A encodes the actin I protein isoform, which is the major actin species of larval intersegmental muscle; also encodes adult cephalic and abdominal muscle (Fyrberg, Mahaffy, Bond and Davidson, 1983, Cell 33: 115-33).

Detailed Mapping Data

FlyBase Computed Cytological Location

Cytogenetic map

Evidence for location

57B5-57B5

Limits computationally determined from genome sequence between P{lacW}l(2)k06409^k06409&P{PZ}insc⁰⁵⁴⁷⁵ and P{PZ}l(2)07806⁰⁷⁸⁰⁶

Experimentally Determined Cytological Location

Cytogenetic map

Notes

References

57A-57A

(determined by in situ hybridisation)

(Drosopoulou et al., 1997)

57A-57C

(determined by in situ hybridisation)

(Meisen et al., 1988)

57A-57A

(determined by in situ hybridisation)

(Fyrberg et al., 1981)

57B-57B

(determined by in situ hybridisation)

(Tobin et al., 1980)

Experimentally Determined Recombination Data

Location

Left of (cM)

Right of (cM)

Notes

Molecular Map Data

Gene Order (in direction of increasing cytology)

References

Gene Order (overall orientation not stated)

References

Gene Model & Products

Please see the GBrowse view of Dmel\Act57B for information on other features

Comments on Gene Model

Atypical cDNA: retained intron (LD04994).

DGC clone LD04994 appears problematic: unspliced intron

Transcript Data

Annotated Transcripts

Name

FlyBase ID

RefSeq ID

Length (nt)

Associated CDS (aa)

Act57B-RA

FBtr0071519

NM_079076

1726

376

Act57B-RB

FBtr0100177

NM_166414

2384

Additional Transcript Data & Comments

Reported size (kB)

1.85, 1.8 (northern blot)

(Tobin et al., 1990)

2 (northern blot)

(Casal and Leptin, 1996)

Comments

External Data

Crossreferences

Polypeptide Data

Annotated Polypeptides

Name

FlyBase ID

Predicted MW (kD)

Length (aa)

Theoretical pI

RefSeq ID

GenBank protein

Act57B-PA

FBpp0071448

41.8

376

5.05

NP_523800

AAF46640

Act57B-PB

FBpp0099533

4.0

6.54

NP_726007

AAM68186

Additional Polypeptide Data & Comments

Reported size (kD)

Comments

External Data

Linkouts

PANTHER - Protein classification by function, families, and pathways

•

FBgn0000044

Crossreferences

InterPro domains - A database of protein families, domains, and functional sites

•

Actin/actin-like (IPR004000)

Actin, conserved site (IPR004001)

Sequences Consistent with the Gene Model

DDBJ /
EMBL /
GenBank

DNA sequence

Protein sequence

Name

P53501

UniProtKB/TrEMBL

Q8SXN3

Maps to

Does NOT map to

Identified with

LD04994

(BDGP Project Members, 2000-)

Mapped Features & Mutations

Please see GBrowse or insertion reports for information on insertions of transgenic constructs and features not listed here

Type

Symbol & Location

Additional Notes

References

rescue fragment

Act57B[+t7BBH]
2R:16,823,313..16,838,903

comment=Act57B[+t7BBH] prevents prepupal lethality in Act57B[ex8]/Df(2R)E2 animals and also partially rescues ellipsoid body morphology in the larval brains of Act57B[ex8] homozygotes; position of restriction fragment on reference sequence inferred by FlyBase curator.
evidence=experimental
linked_to=BamHI-HpaI_rfrag

(Davis et al., 2003)

rescue fragment

Act57B[+tBH]
2R:16,830,445..16,838,903

comment=Act57B[+tBH] prevents prepupal lethality in Act57B[ex8]/Df(2R)E2 animals; position of restriction fragment on reference sequence inferred by FlyBase curator.
evidence=experimental
linked_to=BamHI-HpaI_rfrag

(Davis et al., 2003)

External Data

Linkouts

DEDB - Drosophila exon database: splicing graphs

•

1111

Crossreferences

Expression Data

FlyBase-Curated Data

Transcript and
Protein data

Please see the FlyBase Gene Expression Report for details of gene expression from the literature.

Summary of Transcript Expression

Stage

Tissue/Position

Reference

larval stage

larval somatic muscle

(Tobin et al., 1990)

embryonic stage | late

mesoderm

(Casal and Leptin, 1996)

embryonic stage

larval muscle system

(Tobin et al., 1990)

larval stage

pharyngeal muscle

(Tobin et al., 1990)

Marker for

Subcellular Localization

CV Term

Summary of Polypeptide Expression

Stage

Tissue/Position

Reference

Marker for

Subcellular Localization

CV Term

External Data & Images

Linkouts

FLIGHT - Cell culture data for RNAi and other high-throughput technologies

•

FBgn0000044

FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array

•

FBgn0000044

GEO (NCBI) - Gene expression data: microarray and other high-throughput technologies

•

FBgn0000044

BDGP expression data

•

Show all BDGP original images, annotations and embryonic microarray data for Act57B

FlyExpress - Embryonic expression images (BDGP data)

•

Show all FlyExpress processed images for Act57B

Stage(s) 11-12

Stage(s) 13-16

Alleles & Phenotypes

Summary of Allele Phenotypes

Lethality

Allele

lethal | larval stage (with Df(2R)E2)

Act57B^ex8

Other Phenotypes

Allele

mitotic, with Scer\GAL4^{nos.UTR.T:Hsim\VP16}

Act57B^{Scer\UAS.P\T.T:Avic\GFP}

Phenotype manifest in

Allele

germline cyst, with Scer\GAL4^{nos.UTR.T:Hsim\VP16}

Act57B^{Scer\UAS.P\T.T:Avic\GFP}

Classical Alleles ( 1 )

For All Classical Alleles Show

Allele of Act57B	Class	Mutagen	Stocks	Known lesion
Act57B^ex8		Δ2-3	0	--

Alleles Carried on Transgenic Constructs ( 4 )

For All Alleles Carried on Transgenic Constructs Show

Allele of Act57B	Mutagen	Stocks	Known lesion
Act57B^+t7BBH	in vitro construct \| genomic fragment	0	Yes
Act57B^+tBH	in vitro construct \| genomic fragment	0	Yes
Act57B^GD6854	in vitro construct \| RNAi in vitro construct \| regulatory fusion	1	Yes
Act57B^{Scer\UAS.P\T.T:Avic\GFP}	in vitro construct \| regulatory fusion in vitro construct \| coding region fusion	2	Yes

Aneuploid Aberrations

Useful deficiency

Df(2R)E2

Useful duplication

Dp(2;Y)53D;57C

Disrupted in

Df(2R)E2

(Davis et al., 2003)

Df(2R)Exel6071

(Ryder, 2004.4.26)

Df(2R)Exel7166

(Ryder, 2004.4.26)

Partially disrupted in

Df(2R)ex8

(Davis et al., 2003)

Transgenic Constructs & Insertions

Transgenic Constructs

Type of construct

Name

Expression data

characterization construct

P{Act57B.tBH}

P{Act57B::Act88F^cFa}

reporter construct

P{Act57B-lacZ.+100+831}

P{Act57B-lacZ.-+2}

P{Act57B-lacZ.-1484+2}

P{Act57B-lacZ.-1742+2}

P{Act57B-lacZ.-2479+2}

P{Act57B-lacZ.-278+2}

P{Act57B-lacZ.-593+2.mMEF2}

P{Act57B-lacZ.-593+2}

P{Act57B-lacZ.-593-245}

UAS construct

P{GD6854}

P{UASp-GFP.Act57B}

Insertions

Type of insertions

Name

Expression data

miscellaneous insertions

P{SUPor-P}KG08847

insertion of enhancer trap

P{lacW}F5

Related Comments

Please look at the allele reports for the complete phenotype data

Translation of actin RNA in early embryos injected with initiation factors has been studied.

(Hongo and Jacobs-Lorena, 1991)

RNAi screen using dsRNA made from templates generated with primers directed against this gene causes a binucleation phenotype when assayed in Kc₁₆₇ cells.

(Eggert et al., 2004)

Gene Ontology: Function, Process & Cellular Component ( 10 )

Molecular Function

CV term

Evidence

References

ATP binding

inferred from electronic annotation with InterPro:IPR004001

(FlyBase Curators et al., 2004-)

glucuronosyltransferase activity

non-traceable author statement

(Wilson, 2002)