Gene Dmel\Act42A

Comments

External Data

Crossreferences

Polypeptide Data

Annotated Polypeptides

Name

FlyBase ID

Predicted MW (kD)

Length (aa)

Theoretical pI

RefSeq ID

GenBank protein

Act42A-PA

FBpp0085365

41.8

376

5.16

NP_523625

AAF57294

Additional Polypeptide Data & Comments

Reported size (kD)

Comments

External Data

(BDGP Project Members, 2000-)

PANTHER - Protein classification by function, families, and pathways

•

FBgn0000043

Crossreferences

InterPro domains - A database of protein families, domains, and functional sites

•

Actin/actin-like (IPR004000)

Actin, conserved site (IPR004001)

Sequences Consistent with the Gene Model

DDBJ /
EMBL /
GenBank

DNA sequence

Protein sequence

Name

P02572

UniProtKB/TrEMBL

Maps to

Does NOT map to

Identified with

(Oliver, 2001.8.16)

LD18090

D1366

(BDGP Project Members, 1994-1999)

Mapped Features & Mutations

Please see GBrowse or insertion reports for information on insertions of transgenic constructs and features not listed here

Type

Symbol & Location

Additional Notes

References

External Data

DEDB - Drosophila exon database: splicing graphs

•

11601

Crossreferences

EPD - Eukarytoic Promoter Database, an annotated collection of POL II promoters

•

15007

Expression Data

FlyBase-Curated Data

Transcript and
Protein data

Please see the FlyBase Gene Expression Report for details of gene expression from the literature.

Summary of Transcript Expression

Stage

Tissue/Position

Reference

embryonic stage | blastoderm

embryonic stage

embryonic/larval midgut

embryonic stage

gonad

embryonic stage

supraoesophageal ganglion

embryonic stage

ventral nerve cord

embryonic stage

embryonic/larval proventriculus

Marker for

Subcellular Localization

CV Term

Summary of Polypeptide Expression

Stage

Tissue/Position

Reference

Marker for

Subcellular Localization

CV Term

External Data & Images

(Stroschein-Stevenson et al., 2006)

FLIGHT - Cell culture data for RNAi and other high-throughput technologies

•

FBgn0000043

FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array

•

FBgn0000043

GEO (NCBI) - Gene expression data: microarray and other high-throughput technologies

•

FBgn0000043

Alleles & Phenotypes

Summary of Allele Phenotypes

Phenotype manifest in

Allele

Classical Alleles ( 4 )

For All Classical Alleles Show

Allele of Act42A	Mutagen	Stocks	Known lesion
Act42A^DG03205		1	--
Act42A^EP2096	P-element activity	1	--
Act42A^EY05608	P-element activity	1	--
Act42A^d06237	P-element activity	1	--

Alleles Carried on Transgenic Constructs ( 3 )

For All Alleles Carried on Transgenic Constructs Show

Allele of Act42A	Mutagen	Stocks	Known lesion
Act42A^Act5C.PW	in vitro construct \| regulatory fusion	0	Yes
Act42A^GD1350	in vitro construct \| RNAi in vitro construct \| regulatory fusion	1	Yes
Act42A^{Scer\UAS.P\T.T:Avic\GFP}	in vitro construct \| regulatory fusion in vitro construct \| coding region fusion	2	Yes

Aneuploid Aberrations

Useful deficiency

Df(2R)nap1

Useful duplication

Dp(2;2)BG

Disrupted in

Df(2R)Exel7085

(Ryder, 2004.4.26)

Transgenic Constructs & Insertions

Transgenic Constructs

Type of construct

Name

Expression data

UAS construct

P{GD1350}

P{UASp-GFP.Act42A}

characterization construct

P{Act42A::Act88F^42A}

P{Act42A::Act88F^cFa}

P{Act5C-Act42A.W}

Insertions

Type of insertions

Name

Expression data

miscellaneous insertions

P{wHy}Act42A^DG03205

insertion of mobile activating element

P{EPgy2}Act42A^EY05608

P{EP}Act42A^EP2096

P{GSV1}EP-319^EP-319

P{XP}Act42A^d06237

Related Comments

Please look at the allele reports for the complete phenotype data

S2 cells treated with dsRNA generated against this gene show reduced phagocytosis of Candida albicans compared to untreated cells.

The expression of HIS-C genes during oogenesis has been studied, and compared to periods of DNA synthesis and actin expression during this developmental stage.

(Ruddell and Jacobs-Lorena, 1985)

Translation of actin RNA in early embryos injected with initiation factors has been studied.

(Hongo and Jacobs-Lorena, 1991)

dsRNA directed against this gene causes defects in cytokinesis when tested in an RNAi screen in S2 cells.

(Echard et al., 2004)

Gene Ontology: Function, Process & Cellular Component ( 7 )

Molecular Function

CV term

Evidence

References

ATP binding

inferred from electronic annotation with InterPro:IPR004001

(FlyBase Curators et al., 2004-)

protein binding

inferred from electronic annotation with InterPro:IPR004000

(FlyBase Curators et al., 2004-)

structural constituent of cytoskeleton

inferred from sequence or structural similarity

(Goldstein and Gunawardena, 2000)

inferred from sequence or structural similarity

(FlyBase, 1992-)

Biological Process

CV term

Evidence

References

cytokinesis

inferred from mutant phenotype

(Echard et al., 2004)

cytoskeleton organization and biogenesis

inferred from sequence or structural similarity

(Goldstein and Gunawardena, 2000)

phagocytosis, engulfment

inferred from mutant phenotype

(Stroschein-Stevenson et al., 2006)

Cellular Component

CV term

Evidence

References

actin filament

inferred from sequence or structural similarity

(Goldstein and Gunawardena, 2000)

non-traceable author statement

(Swiss-Prot Project Members, 1986.7.21)

Sequence Ontology: Class of Gene

protein_coding_gene

nuclear_gene

Interactions & Pathways

Summary of Genetic Interactions

Interacts with

Please look at the allele data for full details of the genetic interactions

Act42A allele

Gene

References

Act42A^EP2096

Syx1A

unspecified

CG9894

MESR4

Pdk

EP2

EP364

EP1244

External Data

BioGRID - Interaction data, including yeast 2-hybrid and genetic interactions

•

61432

Orthologs

Genome-wide drosophilid orthologs

Dana\GF13827

Dere\GG10840

Dgri\GH20408

Dmoj\GI19595

Dper\GL17787

Dpse\GA24908

Dsec\GM16492

Dsim\GD10342

Dvir\GJ18395

Dwil\GK21751

Dyak\GE19434

Curated drosophilid orthologs

Dbuz\Act42A

(Laayouni et al., 2000)

Dpse\GA11361

Dvir\ActC1