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Gene Dmel\acj6

General Information
Symbol	Dmel\acj6	Species	D. melanogaster
Name	abnormal chemosensory jump 6	Annotation symbol	CG9151
Feature type	protein_coding_gene	FlyBase ID	FBgn0000028
Created / Updated	2005-01-10/2005-01-10
Genomic Location
Chromosome (arm)	X	Recombination map	1-49.4
Cytogenetic map	13B8-13C1	Sequence location	X:15,246,390..15,272,487 [-]
Map ( GBrowse )	Decorated FastA GenBankGFF Gene region Extended Gene region CDSIntronsExonsTranslationsTranscripts3' UTR5' UTR
Summary Information
Automatically generated summary See sections below for more information	The gene abnormal chemosensory jump 6 is referred to in FlyBase by the symbol acj6 (CG9151, FBgn0000028). It has the cytological map location 13B8-13C1. Its sequence location is X:15246390..15272487. Its molecular function is described as: protein serine/threonine phosphatase activity; DNA binding; transcription corepressor activity; RNA polymerase II transcription factor activity; sequence-specific DNA binding; transcription factor activity. It is involved in the biological processes: chemosensory jump behavior; response to chemical stimulus; synaptic target recognition; dendrite morphogenesis; axon guidance; regulation of transcription, DNA-dependent. 13 alleles are reported. The phenotypes of these alleles are annotated with: lamina plexus; antennal sensillum; maxillary palp sense organ; monopolar laminar cell L1; monopolar laminar cell L3; maxillary palp sensillum basiconicum; antennal glomerulus; neuromuscular junction; bouton. It has 5 annotated transcripts and 5 annotated polypeptides.
External Summaries
Detailed Mapping Data
FlyBase Computed Cytological Location
Cytogenetic map Evidence for location 13B8-13C1   Limits computationally determined from genome sequence between P{EP}hiwEP1308 and P{EP}Ahcy13EP1007
Experimentally Determined Cytological Location
Cytogenetic map Notes References 13C1-13C1   (Clyne, 1998.8.25)
Experimentally Determined Recombination Data
Location	1-49.4 (Carlson, 1993)
Left of (cM)
Right of (cM)
Notes
Molecular Map Data
Gene Order (in direction of increasing cytology) References Gene Order (overall orientation not stated) References
Gene Model & Products
Please see the GBrowse view of Dmel\acj6 for information on other features
Comments on Gene Model
Transcript Data
Annotated Transcripts
Name FlyBase ID RefSeq ID Length (nt) Associated CDS (aa) acj6-RA FBtr0074015  NM_080137   2080   396 acj6-RC FBtr0100326  NM_001014744   1996   368 acj6-RD FBtr0100328  NM_001014743   2074   394 acj6-RE FBtr0290063     2017   375 acj6-RF FBtr0290064     1984   364
Additional Transcript Data & Comments
Reported size (kB)	1.7 (northern blot) (Treacy et al., 1991)
Comments	Alternative splicing of the acj6 gene results in transcripts which encode two forms of the acj6 protein that differ by two basic amino acid residues within the N-terminus of the POU homeodomain. Both types of mRNA produced by alternative splicing were found to be present throughout development with no apparent developmental regulation of splicing. (Treacy et al., 1992, Treacy et al., 1992)
External Data
Crossreferences
Polypeptide Data
Annotated Polypeptides
Name FlyBase ID Predicted MW (kD) Length (aa) Theoretical pI RefSeq ID GenBank protein acj6-PA   FBpp0073832   43.7   396   9.54     NP_524876   AAF48447 acj6-PC   FBpp0099732   40.4   368   9.36     NP_001014744   AAX52497 acj6-PD   FBpp0099734   43.4   394   9.19     NP_001014743   AAX52498 acj6-PE   FBpp0288502   41.3   375   9.36       acj6-PF   FBpp0288503   40.1   364   9.81
Additional Polypeptide Data & Comments
Reported size (kD)	369 (aa) (Treacy et al., 1992) 367 (aa); 44 (kD) (Treacy et al., 1991)
Comments	A splice variant of the acj6 gene (previously called Twin of I-POU), has 2 additional basic residues (RK) with respect to other isoforms which allows it to specifically bind DNA. This form of the acj6 protein does not interact with vvl protein but can act as a positive transcription factor on targets distinct from those regulated by vvl protein. Two different isoforms of acj6 protein appear to exert complementary functions, simultaneously activating and inhibiting two distinct sets of transcription units. (Treacy et al., 1992) A partial peptide which results from alternative splicing and gives rise to a homeodomain that is two amino acids longer than its alternative Ipou protein. (FlyBase, 1992-) acj6 protein cannot bind DNA due to the lack of two basic amino acids in the N-terminal region of its POU homeodomain that are present in other POU doman genes. Immunoprecipitation and crosslinking studies show that acj6 protein associates with vvl protein in vivo. When bound by acj6 protein as a heterodimer, vvl protein no longer binds the enhancer site of the Ddc gene. Transfection experiments in CV-1 cells demonstrate that vvl protein activates transcription of a reporter gene driven by the Ddc promoter or a heterologous promoter preceded by the vvl element. This activation does not occur in the presence of acj6 protein. (Treacy et al., 1991)
External Data
Linkouts	PANTHER - Protein classification by function, families, and pathways • FBgn0000028
Crossreferences	InterPro domains - A database of protein families, domains, and functional sites • POU (IPR013847) POU-specific (IPR000327) Homeobox (IPR001356) Homeodomain-like (IPR009057) Lambda repressor-like, DNA-binding (IPR010982) Homeodomain-related (IPR012287) TRANSFAC - Eukaryotic transcription factors, their genomic binding sites, and DNA-binding profiles • T01898 T01899
Sequences Consistent with the Gene Model
DDBJ / EMBL / GenBank	DNA sequence Protein sequence Name AE003498 AAF48447   AE003498 AAN09663   AF086816 AAC35369   AI945848   AY060272   BF486783   BF502015   BI627120   BT028815   BT028848   CO327308   CO327740   CO341205   CO342904   CZ490303   CZ490304   EC074795   EC235975   EC254147   EC258253   EC258784   EL870865   EL870941   EL871023   ES688842   ES688995   ES691442   ES691459   ES691469   ES691470   ES691476   ES691479   ES691510   ES691513   ES691517   ES691529   ES691976   ES691977   ES691981   ES694691   ES694725   ES694735   S82267   S82271 AAB21441   X58436 CAA41342
UniProtKB/Swiss-Prot	P24350
UniProtKB/TrEMBL
Maps to
Does NOT map to
Identified with	D1371 (BDGP Project Members, 1994-1999)
Mapped Features & Mutations
Please see GBrowse or insertion reports for information on insertions of transgenic constructs and features not listed here Type Symbol & Location Additional Notes References
External Data
Linkouts	DEDB - Drosophila exon database: splicing graphs • 2875
Crossreferences
Expression Data
FlyBase-Curated Data
Transcript and Protein data	Please see the FlyBase Gene Expression Report for details of gene expression from the literature.
Summary of Transcript Expression
Stage Tissue/Position Reference  embryonic stage  ventral nerve cord (Treacy et al., 1991)  embryonic stage  EL neuron (Treacy et al., 1991)  embryonic stage  supraoesophageal ganglion (Treacy et al., 1991)  embryonic stage  RP1 neuron (Treacy et al., 1991)  embryonic stage  anterior corner cell (Treacy et al., 1991)  embryonic stage  posterior corner cell (Treacy et al., 1991)  embryonic stage  embryonic central nervous system (Treacy et al., 1991)  embryonic stage  CQ neuron (Treacy et al., 1991)
Marker for
Subcellular Localization
CV Term
Summary of Polypeptide Expression
Stage Tissue/Position Reference
Marker for
Subcellular Localization
CV Term
External Data & Images
Linkouts	FLIGHT - Cell culture data for RNAi and other high-throughput technologies • FBgn0000028 FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array • FBgn0000028 GEO (NCBI) - Gene expression data: microarray and other high-throughput technologies • FBgn0000028
Alleles & Phenotypes
Summary of Allele Phenotypes
Lethality Allele viable acj62 acj63 acj65 acj66 acj6f07555 lethal | recessive acj6PG63 lethal | larval stage, with Scer\GAL4Cha.7.4 acj61,4.Scer\UAS lethal | pupal stage, with Scer\GAL4Cha.7.4 acj61,4.Scer\UAS Other Phenotypes Allele behavior defective | recessive acj61 acj62 acj63 acj65 acj66 behavior defective | larval stage acj61 chemosensitive behavior defective | recessive acj61 acj6n olfaction defective acj61 olfaction defective | recessive acj61 acj62 acj63 acj65 acj66 locomotor behavior defective | larval stage | recessive acj61 acj66 locomotor behavior defective | adult stage (with acj61) acj66 locomotor behavior defective | adult stage (with acj66) acj61 locomotor behavior defective | adult stage | recessive acj61 acj66 neurophysiology defective | recessive acj61 acj62 acj63 acj65 acj66 neurophysiology defective | somatic clone acj66 neurophysiology defective, with Scer\GAL4GH146 acj61,4.Scer\UAS neuroanatomy defective acj66 neuroanatomy defective | cell autonomous | male acj66 neuroanatomy defective | cell autonomous | somatic clone acj66 neuroanatomy defective | cell non-autonomous | male acj66 neuroanatomy defective | cell non-autonomous | somatic clone acj66 neuroanatomy defective | recessive acj66 neuroanatomy defective, with Scer\GAL4sca-537.4 acj61,3,4.Scer\UAS chemical sensitive acj61 Sterility Allele fertile acj6f07555 Phenotype manifest in Allele lamina plexus (with acj66) acj61 antennal sensillum acj61 maxillary palp sense organ acj61 anterior fascicle & motor neuron, with Scer\GAL4sca-537.4 acj61,3,4.Scer\UAS abdominal ventral longitudinal muscle 3 & neuromuscular junction, with Scer\GAL4sca-537.4 acj61,3,4.Scer\UAS abdominal ventral longitudinal muscle 4 & neuromuscular junction, with Scer\GAL4sca-537.4 acj61,3,4.Scer\UAS abdominal ventral longitudinal muscle 1 & neuromuscular junction, with Scer\GAL4sca-537.4 acj61,3,4.Scer\UAS abdominal ventral longitudinal muscle 2 & neuromuscular junction, with Scer\GAL4sca-537.4 acj61,3,4.Scer\UAS neuromuscular junction | ectopic, with Scer\GAL4sca-537.4 acj61,3,4.Scer\UAS bouton | ectopic, with Scer\GAL4sca-537.4 acj61,3,4.Scer\UAS motor neuron & embryo, with Scer\GAL4sca-537.4 acj61,4.Scer\UAS antennal glomerulus & neuron, with Scer\GAL4GH146 acj61,4.Scer\UAS olfactory sensory organ & neuron & dendrite, with Scer\GAL4GH146 acj61,4.Scer\UAS lamina plexus (with acj61) acj66 lamina plexus acj66 monopolar laminar cell L1 acj66 monopolar laminar cell L3 acj66 monopolar laminar cell L1 & synapse acj66 monopolar laminar cell L3 & synapse acj66 antennal glomerulus & neuron | somatic clone acj66 antennal glomerulus VA3 & neuron | somatic clone acj66 antennal glomerulus VM2 & neuron | somatic clone acj66 antennal glomerulus DM6 & neuron | somatic clone acj66 antennal glomerulus DL1 & neuron | somatic clone acj66 antennal glomerulus D & neuron | somatic clone acj66 antennal glomerulus VA1 & neuron | somatic clone acj66 olfactory sensory organ & neuron & dendrite | somatic clone acj66 maxillary palp sensillum basiconicum acj66 antennal glomerulus | male acj66 axon & olfactory neuron & antennal glomerulus DA4 | male | cell autonomous acj66 axon & olfactory neuron & antennal glomerulus DM3 | male | cell autonomous acj66 axon & olfactory neuron & antennal glomerulus VA6 | male | cell autonomous acj66 axon & olfactory neuron & antennal glomerulus VC2 | male | cell non-autonomous acj66 axon & olfactory neuron & antennal glomerulus V | male | cell non-autonomous acj66 axon & olfactory neuron | male | ectopic acj66 axon & olfactory neuron & antennal glomerulus DA4 | somatic clone | cell autonomous acj66 axon & olfactory neuron & antennal glomerulus DM3 | somatic clone | cell autonomous acj66 axon & olfactory neuron & antennal glomerulus VA6 | somatic clone | cell autonomous acj66 axon & olfactory neuron & antennal glomerulus VC2 | somatic clone | cell non-autonomous acj66 axon & olfactory neuron & antennal glomerulus V | somatic clone | cell non-autonomous acj66 axon & olfactory neuron | somatic clone | ectopic acj66 olfactory neuron & maxillary palp sense organ acj66
Classical Alleles ( 9 )
For All Classical Alleles Show
Allele of acj6 Class Mutagen Stocks Known lesion acj61 loss of function, hypomorph ethyl methanesulfonate 0 -- acj62 loss of function X ray 0 Yes acj63 X ray 0 Yes acj65 X ray 0 Yes acj66 loss of function ethyl methanesulfonate 0 -- acj6PG63 Δ2-3 0 -- acj6f07555 piggyBac transposase 2 -- acj6n amorph 0 -- acj6unspecified 0 --
Alleles Carried on Transgenic Constructs ( 4 )
For All Alleles Carried on Transgenic Constructs Show
Allele of acj6 Class Mutagen Stocks Known lesion acj61,3,4.Scer\UAS in vitro construct | regulatory fusion 0 Yes acj61,4.Scer\UAS in vitro construct | regulatory fusion 0 Yes acj6GD17029 in vitro construct | RNAi in vitro construct | regulatory fusion 1 Yes acj6GD4681 in vitro construct | RNAi in vitro construct | regulatory fusion 1 Yes
Aneuploid Aberrations
Useful deficiency	T(1;3)143-3
Useful duplication	Dp(1;1)M48.6
Disrupted in	Df(1)M34-1Cs (Clyne et al., 1999)
Duplicated in	Dp(1;2)eagx6 (Clyne et al., 1999)
Transgenic Constructs & Insertions
Transgenic Constructs
	Type of construct Name Expression data UAS construct P{GD17029} NA P{GD4681} NA P{UAS-acj6.1,3,4} NA P{UAS-acj6.1,4} NA
Insertions
	Type of insertions Name Expression data miscellaneous insertions PBac{WH}acj6f07555 NA insertion of enhancer trap binary system P{GawB}acj6PG63 No
Related Comments
	Please look at the allele reports for the complete phenotype data Mutants have an abnormal jump response and an abnormal electroantennagram.   Mutants have an abnormal jump response. (McKenna et al., 1989) Ipou gene product cannot bind DNA as it lacks two basic amino acids in the N-terminal region of its POU homeodomain that are present in other POU doman genes. Ipou is coexpressed in subsets of neurons with Cf1a during development. Ipou forms a highly stable heterodimeric complex with Cf1a and inhibits its ability to bind DNA and activate transcription of Ddc. (Treacy et al., 1991) Using the electroantennogram (EAG) to measure antennal physiology, an adult antennal defect in the olfactory behaviour was found in acj61 flies. (Ayer and Carlson, 1991) An alternative transcript of Ipou is incapable of dimerizing with Cf1a but can act as a positive transcription factor on targets distinct from those regulated by Cf1a. This suggests that the Ipou locus simultaneously generates both a specific activator and inhibitor of gene transcription. (Treacy et al., 1992) acj6 protein does not affect DNA binding by the vvl protein, and no interaction between the acj6 and vvl proteins can be detected. (Turner, 1996) Loss of acj6 function in the larval optic lobe neurons results in disorganised retinal axon targeting and synapse selection. (Certel et al., 2000)
Gene Ontology: Function, Process & Cellular Component ( 13 )
Molecular Function
CV term Evidence References
NOT DNA binding inferred from direct assay (Treacy et al., 1991)
RNA polymerase II transcription factor activity non-traceable author statement (Swiss-Prot Project Members, 1992.3.1)
protein serine/threonine phosphatase activity inferred from sequence or structural similarity with EMBL:U53276 (FlyBase, 1992-)
sequence-specific DNA binding inferred from electronic annotation with InterPro:IPR001356 (FlyBase Curators et al., 2004-)
transcription corepressor activity inferred from genetic interaction with FLYBASE:vvl; FB:FBgn0003995 AND inferred from physical interaction with FLYBASE:vvl; FB:FBgn0003995 (Treacy et al., 1991)
transcription factor activity inferred from electronic annotation with InterPro:IPR000327, InterPro:IPR001356 (FlyBase Curators et al., 2004-)
Biological Process
CV term Evidence References
axon guidance inferred from mutant phenotype (Komiyama et al., 2004)
chemosensory jump behavior inferred from mutant phenotype (McKenna et al., 1989)
dendrite morphogenesis inferred from mutant phenotype (Komiyama et al., 2003) traceable author statement (Grueber and Jan, 2004)
regulation of transcription, DNA-dependent inferred from electronic annotation with InterPro:IPR000327, InterPro:IPR001356 (FlyBase Curators et al., 2004-)
response to chemical stimulus inferred from mutant phenotype (McKenna et al., 1989)
synaptic target recognition inferred from mutant phenotype (Certel et al., 2000)
Cellular Component
CV term Evidence References
nucleus inferred from sequence or structural similarity with WB:unc-86; WP:CE00099 (Treacy et al., 1991) non-traceable author statement (Swiss-Prot Project Members, 1992.3.1)
Sequence Ontology: Class of Gene
	protein_coding_gene   nuclear_gene
Interactions & Pathways
Summary of Genetic Interactions
Interacts with	Please look at the allele data for full details of the genetic interactions acj6 allele Gene References acj66 vvl (Komiyama et al., 2003)
External Data
Linkouts	BioGRID - Interaction data, including yeast 2-hybrid and genetic interactions • 70572 Drosophila PIMRider - The Drosophila Protein Interaction map • 522312
Orthologs
Genome-wide drosophilid orthologs	Dere\GG19406 Dvir\GJ16375
Curated drosophilid orthologs
Linkouts	InParanoid orthologs - Eukaryotic orthologs