Gene Dmel\Abd-B

General Information
Symbol	Dmel\Abd-B	Species	D. melanogaster
Name	Abdominal B	Annotation symbol	CG11648
Feature type	protein_coding_gene	FlyBase ID	FBgn0000015
Created / Updated	2003-12-01/2003-12-01
Genomic Location
Chromosome (arm)	3R	Recombination map	3-58.8
Cytogenetic map	89E4-89E5	Sequence location	3R:12,752,932..12,797,958 [-]
Map ( GBrowse )
Summary Information
Automatically generated summary See sections below for more information	The gene Abdominal B is referred to in FlyBase by the symbol Abd-B (CG11648, FBgn0000015). It has the cytological map location 89E4-89E5. Its sequence location is 3R:12752932..12797958. Its molecular function is described as: specific RNA polymerase II transcription factor activity; transcription factor activity; sequence-specific DNA binding. It is involved in the biological processes described with 29 unique terms, many of which group under: anatomical structure development; organ development; regulation of developmental process; genital disc development; cell motility; open tracheal system development; development of primary male sexual characteristics; gamete generation; organ morphogenesis; segment specification; gonadal mesoderm development; regulation of metabolic process; anterior/posterior axis specification. 203 alleles are reported. The phenotypes of these alleles are annotated with 132 unique terms, many of which group under: adult segment; adult; organ system; thoracic segment; abdominal ventral denticle belt; blastoderm embryo; peripheral nervous system; spiracle; larval abdominal segment; adult mesothoracic segment. It has 5 annotated transcripts and 5 annotated polypeptides.
External Summaries

Phenotypic Description from the Red Book (Lindsley & Zimm 1992)
Gene/Allele symbols may differ from current usage	Abd-B: Abdominal-B (I. Duncan and S. Celniker) Heterozygotes for null alleles show weak anteriorly-directed transformations of A5, A6, and A7. In the male, this results in the presence of a tiny extra tergite in A7 and a loss of pigmentation on the A5 tergite. Heterozygotes are partially to completely sterile in both sexes, but are fertile if a duplication for the BXC [such as Dp(3;5)P5 or Dp(3;1)P68] is present. Hemizygotes and homozygotes are lethal; embryos lack posterior spiracles and filzkorper, have the ventral setal bands of A6, A7 and A8 transformed toward A5 or A4, and develop rudimentary chitinized plates in posterior A8. iab5: infra-abdominal 5 (I. Duncan) Hemizygotes show strong transformation of A5 toward A4, resulting in a loss of black pigment in the A5 tergite of the male. In addition, A6 may be weakly transformed toward A4. When homozygous, iab5301 causes a weak transformation of A3 toward A4 as well as a transformation of A5 to A4. iab6: infra-abdominal 6 (I. Duncan) Hemizygotes show strong transformations of both A5 and A6 toward A4. Males show a loss of pigment on the A5 and A6 tergites and show the development of bristles on the A6 sternite. Some alleles cause weak transformations of A4 toward A5. iab7: infra-abdominal 7 (I. Duncan) Hemizygotes show strong transformations of A5, A6, and A7 toward A4. In males, the A5 and A6 tergites show a loss of pigmentation and an unpigmented A4-type tergite develops in A7. Both A6 and A7 show the development of sternites with bristles. Heterozygotes show a small A7 tergite in the male. Two gain-of-function alleles recorded. iab7Spth (split thorax) heterozygotes display a longitudinal furrow in the mesothorax; iab7SGA heterozygotes causes abdominal structures to develop in the back of the head (Awad, Gausz, Gyurkovics, and Parducz, 1981, Acta Biol. Acad. Sci. Hung. 32: 219-28; Kuhn and Packert, 1988, Dev. Biol. 125: 8-18). iab8: infra-abdominal 8 (I. Duncan and S. Celniker) Hemizygous adult males show strong transformation of A5, A6, A7 toward A4. In addition, an A8 tergite develops which is half the size of a normal tergite. In these males, A5 and A6 tergites show a loss of pigmentation and an unpigmented A4-type tergite develops in A7. A6, A7, and A8 all development sternites, the first two with bristles. iab9: infra-abdominal 9 (I. Duncan and S. Celniker) Adult homozygotes or hemizygotes show absent or defective genitalia and analia in both sexes. Adults homozygous or heterozygous for iab965 show in addition a partial transformation of A6 toward A7. Embryos hemizygous for iab9 mutations show the development of a zone of naked cuticle and a rudimentary ninth abdominal setal belt posterior to the eighth abdominal setal belt. Posterior spiracles are absent in iab965 and iab948 hemizygotes and are defective in iab9Uab1, iab9tuh-3, and iab9Tab hemizygotes. iab965 and iab948, but not the other iab9 mutations, cause transformations of the A8 setal belt (located in anterior A8) toward A7. Mcp: Miscadastral pigmentation (E.B. Lewis) Mcp homozygotes have the fourth (A4) and fifth (A5) abdominal segments transformed to a state intermediate between A5 and A6. Similar, but weaker, tranformations occur in Mcp/+ heterozygotes. Mcp1 can be scored in males by dark pigmentation of the A4 tergite and in females by an effect on the orientation of the lateral bristles of the A4 tergite. Sab: Superabdominal (E.B. Lewis) Sab/+ adults show patchy transformations of A3 and A4 to A5. Homozygote viable and more extreme than heterozygote. Mcp1 Sab1 homozygotes show transformations of A3, A4, and A5 to a state intermediate between A5 and A6. Tab: Transabdominal (E.B. Lewis) Heterozygous adults have a pair of laterally disposed longitudinal stripes of tissue on the second thoracic segment (T2) probably corresponding to tissue from the dorsal sixth (A6) and/or seventh (A7) abdominal segments. These ectopic stripes of tissue are entirely black in males but only partially pigmented in females. Tab/+ flies are virtually sterile, and have a thin seventh tergite in males. Fertility is partially restored in the presence of a duplication for BXC, such as Dp(3;1)P68. When hemizygous, Tab embryos have a reduction of the posterior spiracles and filzkorper, and a tiny ninth abdominal ventral setal belt that appears as a small row of denticles posterior to the A8 setal belt. Mc: Microcephalus Eyes of heterozygote small or absent. Scutellars curve upward. Viability and fertility good. Homozygote usually more extreme than heterozygote but not reliably distinguishable. ale Mc homozygotes completely eyeless; fertile except when crossed to each other (Golubovsky and Zakarov, 1972, DIS 49: 112). Viability of homozygote varies from 100 down to 40%. RK1A.
Detailed Mapping Data
FlyBase Computed Cytological Location
Cytogenetic map Evidence for location 89E4-89E5 Limits computationally determined from genome sequence between P{lacW}CSN5^L4032 and P{EP}MESK4^EP1015
Experimentally Determined Cytological Location
Cytogenetic map Notes References 89E7-89E8 (determined by in situ hybridisation) (Ranz et al., 2001) 89E1-89E8 (determined by in situ hybridisation) (Spradling et al., 1999) 89E1-89E8 (BDGP Project Members, 1994-1999) 89E1-89E2 (determined by in situ hybridisation) (Thali et al., 1988) 89E1-89E4 (determined by in situ hybridisation) (Kuziora and McGinnis, 1988) 89E-89E (determined by in situ hybridisation) (Regulski et al., 1985)
Experimentally Determined Recombination Data
Location	3-58.8 3-58.7 (Kuhn and Woods, 1982)
Left of (cM)	fl (Kuhn et al., 1984)
Right of (cM)	Ubx (Kuhn et al., 1984)
Notes	Abd-B does not map between Sb and Ubx. (Kuhn and Woods, 1982) Abd-B maps closer to Ubx than to fl. (Kuhn et al., 1984)
Molecular Map Data
Gene Order (in direction of increasing cytology) References In direction of increasing cytology: Ubx- abd-A- Abd-B- (Tillib et al., 1999) In direction of increasing cytology: Fas1+ nonA-l+ Cctγ+ CG14906- CG31217- CG31217- Ubx- bxd- Glut3+ abd-A- iab-4+ CG10349+ Abd-B- Actn3- Ahcy89E- (Martin et al., 1995) Gene Order (overall orientation not stated) References
Gene Model & Products
Please see the GBrowse view of Dmel\Abd-B for information on other features
Comments on Gene Model

Transcript Data
Annotated Transcripts
Name FlyBase ID RefSeq ID Length (nt) Associated CDS (aa) Abd-B-RA FBtr0083382 NM_142320 3297 270 Abd-B-RB FBtr0083384 NM_080157 4743 493 Abd-B-RC FBtr0083381 NM_169735 3549 270 Abd-B-RD FBtr0083383 NM_169736 3250 270 Abd-B-RE FBtr0083385 NM_206498 3703 270
Additional Transcript Data & Comments
Reported size (kB)	7.8, 4.7, 4.3, 3.7, 3.3 (northern blot) (Celniker et al., 1989)
Comments
External Data
Crossreferences
Polypeptide Data
Annotated Polypeptides
Name FlyBase ID Predicted MW (kD) Length (aa) Theoretical pI RefSeq ID GenBank protein Abd-B-PA FBpp0082824 30.3 270 10.04 NP_650577 AAF55363 Abd-B-PB FBpp0082826 55.1 493 9.54 NP_524896 AAF55362 Abd-B-PC FBpp0082823 30.3 270 10.04 NP_732180 AAF55364 Abd-B-PD FBpp0082825 30.3 270 10.04 NP_732181 AAN13723 Abd-B-PE FBpp0089276 30.3 270 10.04 NP_996220 AAS65159
Additional Polypeptide Data & Comments
Reported size (kD)	270 (aa) (Ali and Bienz, 1991) 491 (aa); 54, 36 (kD) (Celniker et al., 1989) 55, 30 (kD) (Boulet et al., 1991)
Comments	Antibodies that recognize both the 272aa and 493aa Abd-B proteins were made as well as an antibody specific to the 493aa form of the protein. (Boulet et al., 1991) The expression pattern is a composite pattern for the ABD-BI and ABD-BII proteins. The Abd-B protein homeodomain is no more than 55% identical to any other Drosophila homeodomain. (Celniker et al., 1989, Celniker et al., 1989) Antibodies that recognize both the 272aa and 493aa Abd-B proteins were made. (Boulet et al., 1991) The ABD-BI protein corresponds to ABD-BII with a large N-terminal extension; the two proteins contain the same homeodomain. There are two strong transcriptional activation domains in the common part of the two proteins. The ABD-BI-specific exon contains additional transcriptional activation potential, although it is a weaker transcriptional activator than the ABD-BII, apparently due to inhibitory sequences in the ABD-BI-specific exon. (Ali and Bienz, 1991) The expression pattern is a composite pattern for the ABD-BI and ABD-BII proteins. (Celniker et al., 1989) ABD-BI corresponds to ABD-BII with a large N-terminal extension; the two proteins contain the same homeodomain. There are two strong transcriptional activation domains in the common part of the two proteins. The ABD-BI-specific exon contains additional transcriptional activation potential, although it is a weaker transcriptional activator than the ABD-BII, apparently due to inhibitory sequences in the ABD-BI-specific exon. (Ali and Bienz, 1991) The antibody reported in this paper recognizes both the Abd-B-m and Abd-B-r proteins. (de Lorenzi and Bienz, 1990)
External Data
Linkouts	PANTHER - Protein classification by function, families, and pathways • FBgn0000015
Crossreferences	InterPro domains - A database of protein families, domains, and functional sites • Helix-turn-helix motif, lambda-like repressor (IPR000047) Homeobox (IPR001356) Homeodomain-like (IPR009057) Homeodomain-related (IPR012287) TRANSFAC - Eukaryotic transcription factors, their genomic binding sites, and DNA-binding profiles • T01476
Sequences Consistent with the Gene Model
DDBJ / EMBL / GenBank	DNA sequence Protein sequence Name AE003715 AAF55362 AE003715 AAF55363 AE003715 AAF55364 AE003715 AAN13723 AE003715 AAS65159 AF067865 AF067866 AQ025575 BI233131 BI362519 BI565017 BP540589 BP547015 BP553947 BP556554 BT003498 AAO39502 CO262230 CO275627 CO279732 CO283988 CO291273 CO307767 CO307913 CO308838 CO315042 CO315464 CO324475 CO341540 EC057146 EC060697 EC061182 EC064327 EC066177 EC070506 EC071365 EC072969 EC080348 EC200837 EC203021 EC221120 EC238114 EC238351 EC242419 EL874740 EL874858 EL874986 L07835 M21173 AAA28400 U31961 AAA84402 U31961 AAA84403 X15065 CAA33164 X15080 CAA33186 X15080 CAA33187 X16134 CAA34260 X51663 CAB57859 X51669 X51670 X78982 X78983

UniProtKB/Swiss-Prot	P09087
UniProtKB/TrEMBL	Q86P38 Q9VEQ7 A4V304

Maps to	DS07696 (Gemkow et al., 1998)
Does NOT map to
Identified with	RE47096 (BDGP Project Members, 2000-)
Mapped Features & Mutations
Please see GBrowse or insertion reports for information on insertions of transgenic constructs and features not listed here Type Symbol & Location Additional Notes References point mutation Abd-B[M5] 3R:12,758,399..12,758,399 comment=Site of nucleotide substitution in mutant inferred by FlyBase based on reported amino acid change. The amino acid substitution to a stop codon produces an Abd-B M protein of 118 amino acids instead of the normal 493. evidence=experimental na_change=C12758399T pr_change=Q119@\|Abd-B-PB reported_pr_change=?119@ (Sanchez-Herrero et al., 1985, de Lorenzi and Bienz, 1990, Sanchez-Herrero, 1991, Hendrickson and Sakonju, 1995, Foronda et al., 2005)
External Data
Linkouts	DEDB - Drosophila exon database: splicing graphs • 9720
Crossreferences
Expression Data
FlyBase-Curated Data
Transcript and Protein data	Please see the FlyBase Gene Expression Report for details of gene expression from the literature.
Summary of Transcript Expression
Stage Tissue/Position Reference larval stage (Celniker et al., 1989) embryonic stage \| cellular blastoderm parasegment 13 (Boulet et al., 1991) embryonic stage \| extended germ band stage parasegment 14 (Boulet et al., 1991) adult stage (Celniker et al., 1989) embryonic stage \| 4-10 hr (Celniker et al., 1989) embryonic stage \| 4-7 hr (Celniker et al., 1989) embryonic stage \| extended germ band stage parasegment 14 (Boulet et al., 1991) embryonic stage \| 4-7 hr (Celniker et al., 1989) embryonic stage \| late parasegment 14 <of> embryonic central nervous system (Boulet et al., 1991) embryonic stage \| 10-13 hr (Celniker et al., 1989) embryonic stage \| late parasegment 14 <of> embryonic central nervous system (Boulet et al., 1991) embryonic stage \| late (Celniker et al., 1989) embryonic stage \| cellular blastoderm parasegment 13 (Boulet et al., 1991) embryonic stage \| late (Celniker et al., 1989) embryonic stage \| late epidermis <of> abdominal segment 9 (Boulet et al., 1991) embryonic stage parasegment 10..14 (Boulet et al., 1991) embryonic stage \| late parasegment 11..13 <of> embryonic central nervous system (Boulet et al., 1991) embryonic stage \| late parasegment 14 <of> epidermis (Boulet et al., 1991) embryonic stage \| extended germ band stage parasegment 14 (Boulet et al., 1991) embryonic stage \| cellular blastoderm parasegment 14 (Boulet et al., 1991) embryonic stage parasegment 10..14 (Boulet et al., 1991) larval stage (Celniker et al., 1989) embryonic stage \| late parasegment 11..13 <of> embryonic central nervous system (Boulet et al., 1991) embryonic stage \| 10-13 hr (Celniker et al., 1989) embryonic stage \| late epidermis <of> abdominal segment 9 (Boulet et al., 1991) pupal stage (Celniker et al., 1989) embryonic stage (Roder et al., 1992) embryonic stage \| late parasegment 14 <of> embryonic central nervous system (Boulet et al., 1991) adult stage (Celniker et al., 1989) pupal stage (Celniker et al., 1989)
Marker for

Subcellular Localization
CV Term
Summary of Polypeptide Expression
Stage Tissue/Position Reference embryonic stage \| mid-late proctodeum (Celniker et al., 1989) embryonic stage \| mid-late parasegment 13..15 <of> ectoderm (Celniker et al., 1989) embryonic stage \| mid-late parasegment 13..15 <of> ectoderm (Celniker et al., 1989) embryonic stage \| stage 13 ectoderm <of> parasegment 10-13 (de Lorenzi and Bienz, 1990) embryonic stage \| mid-late visceral mesoderm (Celniker et al., 1989) embryonic stage \| stage 12 parasegment 11-14 (Bachiller et al., 1994) embryonic stage ectoderm (Celniker et al., 1989) embryonic stage \| mid-late parasegment 11..15 <of> mesoderm (Celniker et al., 1989) embryonic stage \| stage 12 parasegment 10-14 (Hendrickson and Sakonju, 1995) embryonic stage \| mid-late Malpighian tubule (Celniker et al., 1989) larval stage \| third instar male genital disc \| restricted (Freeland and Kuhn, 1996) embryonic stage \| mid-late Malpighian tubule (Celniker et al., 1989) larval stage \| third instar female genital disc \| restricted (Freeland and Kuhn, 1996) embryonic stage ectoderm (Celniker et al., 1989) embryonic stage \| stage 12 parasegment 13 (Boulet et al., 1991) embryonic stage \| mid-late parasegment 12..14 <of> embryonic central nervous system \| presumptive (Celniker et al., 1989) embryonic stage \| stage 13-14 visceral mesoderm <of> embryonic/larval hindgut <of>parasegment 13-15 (Tremml and Bienz, 1989) embryonic stage \| stage 11-13 parasegment 11-15 (de Lorenzi and Bienz, 1990) embryonic stage \| stage 11 visceral mesoderm <of> parasegment 11-14 (de Lorenzi and Bienz, 1990) embryonic stage \| late (Lo et al., 2002) embryonic stage \| stage >=12 parasegment 14 (Boulet et al., 1991) embryonic stage \| stage 10 parasegment 13-15 (de Lorenzi and Bienz, 1990) larval stage larval fat body \| posterior (Marchetti et al., 2003) embryonic stage \| stage 13-14 visceral mesoderm <of> embryonic/larval midgut <of>parasegment 11-13 (Tremml and Bienz, 1989) embryonic stage \| mid-late spiracle (Celniker et al., 1989) embryonic stage \| stage >12 parasegment 11..13 (Boulet et al., 1991) embryonic stage \| mid-late spiracle (Celniker et al., 1989) embryonic stage \| mid-late visceral mesoderm (Celniker et al., 1989) embryonic stage \| mid-late parasegment 12..14 <of> embryonic central nervous system \| presumptive (Celniker et al., 1989) embryonic stage central nervous system (Celniker et al., 1989) embryonic stage \| mid-late proctodeum (Celniker et al., 1989) embryonic stage \| mid-late parasegment 11..15 <of> mesoderm (Celniker et al., 1989) embryonic stage central nervous system (Celniker et al., 1989)
Marker for

Subcellular Localization
CV Term	nucleus (Celniker et al., 1989) nucleus (Celniker et al., 1989) nucleus (de Lorenzi and Bienz, 1990)
External Data & Images
Linkouts	FLIGHT - Cell culture data for RNAi and other high-throughput technologies • FBgn0000015 GEO (NCBI) - Gene expression data: microarray and other high-throughput technologies • FBgn0000015 FlyExpress - Embryonic expression images (BDGP data) • Show all FlyExpress processed images for Abd-B Stage(s) 7-8 Stage(s) 9-10 Stage(s) 11-12 Stage(s) 13-16
Alleles & Phenotypes
Summary of Allele Phenotypes
Lethality Allele viable Abd-B^{Fab7:iab8-Plac159Δ} Abd-B^S12 Abd-B^S6 Abd-B^StP5 Abd-B^Uab-1 Abd-B^iab8-rv100 Abd-B^iab8-rv107 Abd-B^iab8-rv111 Abd-B^iab8-rv114 Abd-B^iab8-rv175 Abd-B^iab8-rv185 Abd-B^iab8-rv96 Abd-B^iab9-Tab Abd-B^iab9-tuh-3 Abd-B^iab9-x23-1 viable \| good Abd-B^Mc lethal Abd-B^m.hs Abd-B^r.hs lethal (with Df(3R)P9) Abd-B^Cst Abd-B^iab8-D16 lethal (with Df(3R)StP234) Abd-B^Cst lethal \| recessive Abd-B²⁹⁷ Abd-B³⁸⁰ Abd-B^{HCJ199.Scer\FRT} Abd-B^HCJ199 Abd-B^M3 Abd-B^S11 Abd-B^S1 Abd-B^S2 Abd-B^S3 Abd-B^S4 Abd-B^S7 Abd-B^S8 Abd-B^S9 Abd-B^iab8-D14 Abd-B^iab8-D16 Abd-B^iab8-D3 Abd-B^iab8-D6 Abd-B^iab9-65 lethal \| homeotic Abd-B²⁹⁷ Abd-B^M8 Abd-B^S11 Abd-B^S4 Abd-B^S7 Abd-B^iab8-D16 Abd-B^iab8-D3 Abd-B^iab8-rv10 Abd-B^iab8-rv5 Abd-B^iab8-rv75 lethal \| homeotic \| recessive Abd-B^M1 Abd-B^iab9-Tab lethal \| homeotic \| larval stage \| recessive Abd-B^iab8-Plac159 Abd-B^{iab8-Plac159Δ} lethal \| partially \| recessive Abd-B^S5 Abd-B^iab7-SGA lethal \| larval stage \| recessive Abd-B^Uab-1 Abd-B^iab9-tuh-3 lethal \| larval stage, with Scer\GAL4^pnr-MD237 Abd-B^Scer\UAS.cCa Other Phenotypes Allele visible Abd-B^Fab7-12 Abd-B^Fab7-2 Abd-B^S1 Abd-B^S3 Abd-B^S4 Abd-B^iab8-D16 visible (with Df(3R)P9) Abd-B^iab8,9-T1J visible \| cell autonomous \| homeotic \| recessive \| somatic clone Abd-B^unspecified visible \| recessive Abd-B^Cst Abd-B^{Fab7-2.prom-scs} Abd-B^M5 Abd-B^Uab-1 Abd-B^blt Abd-B^iab7-PRE-135 Abd-B^iab7-PRE-246 Abd-B^iab7-PRE-300 Abd-B^iab7-PRE-316 Abd-B^iab7-PRE-56 Abd-B^iab7-PRE-83 Abd-B^iab8-D14 Abd-B^iab9-48 Abd-B^iab9-65 Abd-B^iab9-Tab Abd-B^iab9-tuh-3 Abd-B^iab9-x23-1 visible \| dominant Abd-B^Fab7-1 Abd-B^{Fab7-2.scs-prom} Abd-B^Fab7-2 Abd-B^Fab7-3 Abd-B^{Fab7:iab8-D14} Abd-B^M1 Abd-B^Mc Abd-B^Mcp-1 Abd-B^{Mcp-B116:Sz:iab8-D16} Abd-B^Mcp-B116 Abd-B^Mcp-H27 Abd-B^PSz-1 Abd-B^PSz-2 Abd-B^Sz Abd-B^iab7-Plac132 Abd-B^iab7-SGA Abd-B^iab8-D14 Abd-B^iab9-65 Abd-B^iab9-Tab Abd-B^iab9-x23-1 Abd-B^lac1 visible \| dominant \| homeotic Abd-B^iab6-G Abd-B^iab7-Spth Abd-B^iab9-Tab visible \| dominant \| male Abd-B^Cst visible \| homeotic Abd-B^F Abd-B^Fab-3,5 Abd-B^Fab-6,7.1