Clone Dmel\LP11751

General Information
Symbol	Dmel\LP11751	Species	D. melanogaster
Name	LP11751	FlyBase ID	FBcl0187539
Feature type	cDNA_clone	Created / Updated	2005-11-01/2006-08-19
Computed gene(s)

Collection Status	BDGP
Known Problems	incomplete CDS
Library	LP
Strain	y; cn bw sp
Tissue Source	larval stage whole organism & pupal stage whole organism
Vector	pOT2
Sequence Data of the Insert
Full Length Sequence
Total bases	1989
GenBank	AY061566
Full Length sequence data	CTCAACGAGGGCAACATCTGCTTTGTGAACTCCCACCTGGCCGCCCACAT GGGCTACGTGGAGGAGCGGAATCAGGACTACAACGCTATCGTGGAGGGCA TACGCTTCGATGATGGCCGAACAATCAGTGACCATGATCACATCTTCTGG GTCGGCGATTTGAATTACCGCATCCAGGAGCCGCCCGGCCAACAGCGACC AGGACCACTGAGCGATGCCCAGACATACGAGCTGCTACTGCAGTACGACC AGCTACGTCAGGAGATGCGGCGTGGAAAGTGCTTTGAGGGCTACACCGAG GGCGAGATCAAGTTCCGGCCGACGTACAAGTATGATCCGGGCACGGACAA CTACGACAGCAGCGAGAAGCAGCGGGCGCCGGCCTACTGCGATCGAGTGC TCTGGAAGGGCACGCGCATCGAGCAGCTGGCCTACAACAGCATCATGGAG ATTCGGCAGAGCGATCACAAGCCCGTGTACGCCGTGTTCCAGGTCAAGGT CAAGACTCGCGATGAGGTCAAGTACAAACGGGTGCAGGAGGAGGTGCTGA AGGCAGTGGACAAGCGGGAGAACGACAACCAGCCACAGATCAATGTCGAT AAGACGGTCATCGATTTCGGGACTGTGCGATTTAACGAGCCGAGCACACG GGACTTCAACGTCTATAACAACTGCCCACTACCTGTGGACTTCAGTTTCA AAGAGAAGGATATACACGCCATCTGCGAGCCATGGCTGCACGTCGATCCG CGCCAAGACTCGCTGCTGATCGACTCCGCCCGAAGCATTCGGCTGAAAAT GAACGCCAATGTGCGCACCATTGCCGGCTTGCTTCGCAAGATCCGTGCTA GCGACAACTTCGATATTCTCATCCTGCACGTGGAGAACGGGCGTGACATC TTCATCACGGTTACGGGCGACTATCAGCCCAGTTGCTTTGGCCTTTCCAT GGAGACGATGTGCCGCACCGACCGTCCATTAAGCGAGTATTCCCAAGATC AGATTAAGCAACTCATGAATGACGAATCACCGGAATACCGTGTGACGATG CCCCGGGAATTCTTTCTGCTGATCGACTATCTGTACAGGCAGGGCTCCAA GCAGGTGGGCGCCTTCCCGTCCTACGACTCGCGTCTCTCCCTCGGCGCTC AATTTAACTCGGTGCGCGATTGGTTGGACACCTGGTCGGATGATCCGTTC CCTGCCAACGCAGAGACGGCTGCGCAGGCGCTGCTGCTTTTGCTGGATCT GCCGGAGCATGCTCTGCTGGAGCCGGTGGTCGAGAATCTGCTGGAGTGCA CCAACAAGTCGCAGGCTATGGACTACATTTCGCTGTTAAGTCCCCCCAAG CGCAATGTTTTCATGCACCTTTGTATGTTCCTGCGCGCGGGCATCGAGAG TCAGTTCTATGATCTGCATCAAGTCGCATCCACCTTTGGCCGCATTCTGT TGCGAAGCACCGAGCGTGCCGCCTGGATGGACTACCACAGCCGATGCATA CAGTTCATGCGGCTCTTCATCGACACCGACGTCGAGGCAATGGGCAACGG CAATGAGGGCGCCGGAACTGGAACAGGCTCGGGATCTGGGACCCGGGCGG GTCTGCAGGCATAGTCGCCGCAGCTGTGCCGCCGAATCGGAAGCGAGCGA TAGATGTACTACTCTGTTGATAGTTACGCATAGTGTTAAGCTATTTATTT TATTTGTTGGTACTTTGTTGGCACTTAGCATTTATATCTTCTCTGTTTTA ACTGTTAATTGTTTGTGCCCGCCCTCCGTTCATTCCAACTCGGTTGTCCC CGCCTGCATCACAAATCCATGGAGACACGCAACCAGCAACGCAAAGATAA ATGAATTGTGTAAATAAGTAATTCGTTTAGCACGATTGTTTGTTAGTTTA AATTAATGAAGTGACCCTCTTACATCCCCGTTTCAGTTGCAACTGAAAAT AAATAACACCGTTACATACAGTAAAAAAAAAAAAAAAAA
Library Information
Comments	mRNA source--varying stages of larvae and early pupae, from an isogenic y; cn bw sp strain, polyA+ selected once. Oligo(dT) primed with XhoI site at end of primer for first strand synthesis; EcoRI adapter on 5' ends of clones; size fractionated on Sephacryl S-500--approximately 1-6kb. cDNA directionally cloned into EcoRI/XhoI-digested pOT2 plasmid. ~500,000 colonies were plated out and a mass plasmid DNA prep was done using Qiagen Plasmids containing the cDNA inserts were size selected on Sephacryl S-500 column in order to exclude plasmids with no insert. Early elutions were collected and transformed into DH5 alpha strain (Gibco/BRL).
Library Synonyms
Reported As
Symbol Synonym	LP (Rubin et al., 2000, )
Library References
Review	Rubin et al., 2000, Science 287(5461): 2222--2224 A Drosophila complementary DNA resource. [FBrf0127297]
External Crossreferences & Linkouts
Linkouts
	DGRC - Stock center for Drosophila cDNAs, vectors, and cell lines • FBcl0187539
Synonyms & Secondary IDs ( 0 )
Reported As
Symbol Synonym
Secondary FlyBase IDs

References ( 1 )
Review	Rubin et al., 2000, Science 287(5461): 2222--2224 A Drosophila complementary DNA resource. [FBrf0127297]