Clone Dmel\LD47929

General Information
Symbol	Dmel\LD47929	Species	D. melanogaster
Name	LD47929	FlyBase ID	FBcl0174728
Feature type	cDNA_clone	Created / Updated	2005-11-01/2006-08-19
Computed gene(s)	Dmel\Sra-1

Collection Status	BDGP
Known Problems	none
Library	LD_pOT2
Strain	y; cn bw sp
Tissue Source	embryonic stage whole organism
Vector	pOT2
Sequence Data of the Insert
Full Length Sequence
Total bases	3615
GenBank	AY122203
Full Length sequence data	AAAAGTCGTGTTGTATTTGTGGCGAAAAAAATTTGTGTCGATTGGATTGG GGCCCCGCCTTGTTAGAATTTAACCATTTCGCGCTGCTGGAGCATGATAA GCAGCCCAGCATGACGGAGAAGATTACGCTAGCCGACGCGCTGTCCAACG TGGAGGTGTTGGACGAGCTATCCCTGCCGGACGAGCAGCCCTGCATCGAG GCGCAGCCCTGCTCGATTATCTACAAGGCAAACTTCGATACGAATTTCGA GGATCGCAATGGATTTGTCACGGGTATCGCCAAGTACATCGAGGAGGCCA CCACCCATGCCAACTTGAATGTGCTCCTGGACGAGGGGCAGAAACACGCA GTAATGCTCTACACCTGGCGCTGCTGCTCGCGCGCCATTCCGCAGCCCAA GTCCAATGAGCAGCCGAATCGCGTGGAGATCTACGAGAAGACGGTCGAGG TGCTAGCCCCGGAGGTGAACAAGCTGCTCAACTTCATGTACTTCCAACGC AAGGCCATCGAGGCCTTCTCCGGCGAGGTGAAGCGCTTGTGCCACGCCGA GAAGCGCAAGGACTTCGTGTCCGAGGCGTACCTATTGACGCTAGGCAAGT TTATCAACATGTTTGCCGTGCTGGACGAGCTGAAGAACATGAAGTCCAGC GTGAAGAACGATTACTCCACCTACAGGCGAGCTGCGCAGTTCCTCAAAGT GATGTCGGACTCGCACACCTTGCAGGAGTCGCAGAATCTGTCCATGTTCC TGGCCACGCAGAACAAGATTCGCGACACGGTCAAGGACACGCTGGAGAAG ATCGTGGGCTACGAGGATCTGCTCTCAGATGTGGTCAACATTTGCGTGCA TATGTTCGAGACAAAGATGTACTTGACCCCTGAGGAGAAGCACATGCTGG TAAAAGTTATGGGCTTTGGCCTGTTCCTTATGGACAGCGATGCGTGCAAT ATCAACAAACTTGATCAAAAGAAGAAGATACGGCTGGATCGCATCGATCG CATCTTTAAGAACCTGGAAGTGGTGCCCCTCTTCGGCGACATGCAGATTG CTCCCTTCAACTACATAAAGCGCAGCAAGCACTTCGATTCCAGCAAGTGG CCGCTGTCCAGCTCAAACGCCATCAGCCCACAGGCGGATCTAATGGTGCA TTTGCCGCAGATACGCGAGGATCACGTCAAGTACATTTCGGAGCTGGCGC GATACACAAACGAGGTGACCACCACCGTCAAGGAGAATCCCTCTGATGCC GAGAACCGGATTACGGCCGACCTGGCTCTGCGCGGCCTTCAATTGCTGTC CGAGTGGACCAGTGTGGTCACCGAGTTGTACTCCTGGAAGCTCCTGCATC CGACTGATCATCACCAGAACAAGGAGTGCCCCGTGGAGGCTGAGGAGTAC GAGCGGGCCACCCGCTACAATTACACCTCGGAAGAGAAGTTCGCGCTGAT CGAGGTCATCGCCATGATAAAGGGACTGCAGGTGCTAATGGCGCGCATTG AAACCGTATTGTGCGAGGCTATCCGGCGTAACATCTATTCCGAGCTGCAG GACTTCGTGCAGCTTTCGCTTCGTGAACCGCTGCGAAAGGCCGTAAAAAA TAAGAAGGATCTTATCCGGAGCATCATCATGTCGGTGCGAGAGACATCGG CGGACTGGCAAAAGGGCTATGAGCCCACCGACGATCCAGTGGCCAAGGGC AAGAAGGATCCCGACGGCGGCTTCCGCATCCAGGTGCCTCGCCTCAATGT GGGACCTTCCTCCACTCAGCTATACATGGTGCGCACCATGCTGGAGTCTC TGATTGCCGACAAAAGCGGCGGCAAGCGCACTTTGCGCAAGGACATCGAC GGAAACTGTTTGCTGCAGATCGACACGTTCCACAAGACATCGTTCTACTG GAGCTACTTGCTCAACTTCAGCGACACGCTTCAGAAGTGCTGCGATCTCT CGCAGCTGTGGTATCGCGAGTTCTACTTGGAGATGACCATGGGTCGTAAG GTTAACAAGTGCTTGGTGCGGCATCAGCACAACGAGGAGTGCAAGGATCT GATCACCATGGAGAAGCGCATTCAGTTTCCGATTGAAATGTCCATGCCCT GGATCCTAACCGATCACATTCTGCAAACGAAGGAGCCCTCAATGATGGAA TTTGTCTTGTATCCCCTGGACTTGTACAACGACTCGGCGTACTACGCACT CACTGTCTTCCGAAAGCAGTTCCTGTACGACGAAGTGGAGGCCGAAGTCA ATCTGTGCTTCGATCAGTTTGTCTACAAGCTGAGCGAGCAGATCTTTGCC CATTACAAGCAATTGGCGGGCAGCATCTTCTTGGACAAGCGATTCCGTTT GGAGTGCGAGGTACTCGGTTTCAACTTCCAGTCGTATCCCCGCAATAATC GTTATGAGACCTTGTTAAAACAACGACATGTCCAACTTTTGGGCCGTTCT ATTGACTTGAACAAACTGATCACACAACGCATAAACGCCAACATGCACAA GAGCATTGAGTTGGCCATCAGTCGTTTCGAGGGCAACGATATAACCGGCA TTGTGGAACTCGAAGGCCTGCTAGAGGCGAATCGAATTTGCCACAAGTTG CTAAGCAAGTACTTGGCCCTGGATAACTTTGACGGCATGGTAAAGGAGGC TAACCACAATGTACTGGCGCCTTACGGCAGAATAACACTGCACGTCTTTG TGGAATTGAACTACGACTTTCTGGTCAATTACTGCTATAATGCAGCCACG AATCGTTTCATTCGAACCAAAGTGAATCTTTCGTCGTCACAGGCTATTCA GCGTGAGAAGCCGCCACAAATGTCCCATTACTATCTGTGGGGTTCCAAGC AGCTGAATGCTGCCTATTCCACACAGTATGGCCAGTACACTGGCTTTGTA GGTTCACCGCATTTCCATGCCATGTGCCGCCTGCTGGGCTATCAGGGCAT AGCAGTCGTCATGGACATTATACTGAAGGACATTGTTAAGCCACTGATCC AGGGCTCGCTGCTGCAGTTCACCAAGACGCTGATGATTGCCATGCCCAAG TCATGCAAACTACCCCGTTGCGAATATGGTTCGCCTGGAGTGCTCAGCTA CTATCAGGCTCATCTTACAGACATCGTGCAATATCCGGACGCGAAGACAG AGCTATTTCAGTCTTTCCGGGAGTTTGGCAACAGCATTATCTTTTGCCTG CTGATCGAACAGGCGCTCTCGCAGGAGGAGGTGTGCGATCTACTGCACGC CGCCCTCTTCCAGAACATCTTTCCCAGACCTTTTTGCAAAGAGAATGAAA AACCGGAGGCTAAGCAAAAGCGTCTTGAAGCACAGTTTGCCAATCTGCAG ATAGTTTCAAACGTGGAAAAGATTGGCACTGCAAAGCAAGCAATGATAGC ACGAGAGGGAGATTTGCTGACTCGTGAGCGTCTTTGCTGTGGCCTCAGCA TTTTTGAAGTTATTCTCAACCGCGTGAAGAGCTATTTGGACGACCCTGTG TGGTGTGGTCCTCCACCGGCCAACGGGATTATCCACGTGGATGAGTGCTC GGAGTTCCATCGCCTTTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAA
Library Information
Comments	mRNA source--0-22hr embryos, from an isogenic y; cn bw sp strain, polyA+ selected twice. cDNA made using Stratagene ZAP-cDNA synthesis kit; oligo(dT) primed with XhoI site at end of primer for first strand synthesis; EcoRI adapter on 5' ends of clones; size fractionated on Sephacryl S-500--approximately 1-6kb. For clones LD01001-LD21096, cDNA directionally cloned into EcoRI/XhoI-digested Stratagene Uni-Zap XR vector, which allows in vivo excision and recircularization of pBluescript SK(+/-) plasmid. For clones LD21101 and higher, cDNA directionally cloned into EcoRI/XhoI-digested pOT2 plasmid.
Library Synonyms
Reported As
Symbol Synonym	LD (Rubin et al., 2000) LD_pOT2 (Rubin et al., 2000, )
Library References
Review	Rubin et al., 2000, Science 287(5461): 2222--2224 A Drosophila complementary DNA resource. [FBrf0127297]
External Crossreferences & Linkouts
Linkouts
	DGRC - Stock center for Drosophila cDNAs, vectors, and cell lines • FBcl0174728
Synonyms & Secondary IDs ( 0 )
Reported As
Symbol Synonym
Secondary FlyBase IDs

References ( 1 )
Review	Rubin et al., 2000, Science 287(5461): 2222--2224 A Drosophila complementary DNA resource. [FBrf0127297]