Clone Dmel\LD22664

General Information
Symbol	Dmel\LD22664	Species	D. melanogaster
Name	LD22664	FlyBase ID	FBcl0167155
Feature type	cDNA_clone	Created / Updated	2005-11-01/2006-08-19
Computed gene(s)	Dmel\pcm

Collection Status	DGC gold BDGP
Known Problems	none
Library	LD_pOT2
Strain	y; cn bw sp
Tissue Source	embryonic stage whole organism
Vector	pOT2
Sequence Data of the Insert
Full Length Sequence
Total bases	5220
GenBank	AY051675
Full Length sequence data	CCTCGCCTACTTGCAACTTTTGTGTCAATCAATACATGTTGAAAACAGAT AAAGACACAAATTTTTCAGTTCATCTAAATTGCCACTGATCCAGGCAGAA CAGCACGGCGGCAGAACAGTCCCATCCAGAACAGCGACATGGGCGTTCCC AAGTTCTTTCGCTACATCAGCGAGCGATATCCGTGCCTCAGCGAATTGGC GCGGGAGCACTGCATACCCGAGTTCGACAATCTGTACTTGGACATGAACG GCATTGTCCACAACTGCTCGCATCCGGACGACAACAACATACACTTTCAC CTGGAGGAGGAGCAGATATTCCAGGAAATCTTCAACTACGTAGACAAGCT GTTCTACCTGATCAAGCCGCAGCGCCTCTTCTTCCTTTCAGTTGACGGGG TGGCTCCACGGGCCAAGATGAACCAGCAGCGCAGCCGGCGATTCCGGACG GCGCGCGAGGCGGAGCAGCAGGAGGCCAAGGCGGCCCAGCGAGGCGAGTT GCGAGAGCACGAGCGCTTCGACAGCAACTGCATTACGCCGGGCACCGAAT TCATGGTGCGTCTGCAGGAGGGACTGCGCGCCTTTCTCAAAACGAAGATT TCGACGGATCCGCTGTGGCAGCGTTGCACCGTAATCCTAAGTGGCCAGGA GGCGCCCGGCGAGGGCGAGCACAAGATCATGGACTACATTCGGTACATGA AGACGCAGCCGGACTATGATCCCAATACACGACACTGCCTTTACGGCCTG GACGCCGATCTGATCATTCTGGGCCTCTGCACCCACGAGCTGCACTTCGT GGTGCTGCGCGAGGAGGTCAAGTTCGGGCGCAACGTCAAGCGCACCTCGG TGGAGGAGACGCGCTTCTTCCTGCTGCACCTGGGTCTGCTGCGCGAGTAC CTCGAGCTGGAGTTCGACGCCCTGAGAACAGACGAGCACAAATTGGACAT TGCGCAGCTTATCGACGACTGGGTGCTGATGGGCTTTTTGGTCGGCAACG ACTTCATTCCCCACCTGCCGTGCCTGCACATTTCATCAAATGCACTGCCG CTGCTCTACCGCACCTACATCGGCATTTACCCGACCCTCGGCGGCAACAT CAACGAGAACGGCAAGCTCAACCTGCGCCGCTTGCAGATTTTCATATCGG CCCTCACCGAGGTGGAGCTGGATCACTTCAAGGAGCACGCCGACGACTTG AAGTACATGAACAACAAATCAGAGGCGTTCGACATGGACGTCGGCGAGAT CACCGAGTCTCAAAACCTCGACTCCGATCTCGGAGCCCTCATCAACAAGA GCATGCTGCTCTACGATGACGACAGCGAGGAGGACTGCTCCGACGAGAAC GCGGTTCTTCTGAAGGAGTTCCAGAACTACAAGCGAAACTTCTACCGCAA CAAGTTCAAGCGTGATCCGAATGATGAACTGATCGAGGAACTGTGCCACC ACTACGTCAATGCGCTGCAGTGGGTGCTCGACTACTACTACCGTGGCGTG CAGTCCTGGGACTGGTACTATCCCTTCCACTACACTCCATTCATCAGCGA CCTCAAGAACATCGAGCAGGTGGAGATCGCCTTCCACATGGGCACGCCCT TTCTGCCATTCCAGCAGCTTCTGGCGGTACTGCCGGCGGCCAGTGCAAAG CTTCTCCCTGTCGCCTACCACGACCTCATGCTGCTGCCGACCAGCCCGCT GGCCGAGTTCTATCCACTGGAGTTCGAGAGCGACCTCAATGGCAAGAAAC ACGATTGGGAGGCGGTTGTGCTGATACCCTTCATCGATGAGGGTCGGCTG CTGGCCGCTATGCTGCCATGCGAGGCTCAGCTGTCGCTGGAGGAGCGCGA ACGCAACCGACACGGTCCCATGTATGTGTACAAATACAGTACGGTGGCGC AGGGTCCCATGCCAGCGTATCCACCGCTGAGGGCTCTTCCCGTGCTCTAC TGCACGGAGGTGGCCAAGTGGTCGCACGAAATCGCAGTCAATCTGCCGTA CAGCGTGTGCATCGAGCTACCAAATGCGGCGAGAACCGTTTTCTTCCCGG GATTTCCCACCATGCAGCATCTGCCCTTCGATTTCGAGCTGCGCAACGAC CGCGTCAAGGTGTTCGAACAGGTCAGTCGCAACCAGAACATTGTGCTAAA ACCACGCAAGCGCCAGCTGGAGGACACACTGACGGCGGTGGCCTCCCAGT ACCTCGGGAAGGTGATCCACGTCGGCTGGCCGCATCTCGTCAAGGCGATA GTGGTGCGCGTGGCCACGCGAGATCAGCGCGTCGACAGCGAGGGAATCAC GCTAAACGATTCGCGGCGTTTCGACTCGGAATGCAAGGCACTGCAGGAAC ACTTTATCAATCGTATGGGAATACAGTTTGCCAACTATGATGTGCTCGTC TATGTGCGCACCTTTGCCGGCAATTCGACGGAGTTCCGCGACAAGGGGGC ATTGATGGTGCGCGACTCGTGGAGCAGCTCCGTCACCGGCTATCCAGCCC AGGGCGTGGTGGCCGATCTGACTGTGTGGGAAAGAATGCGCAAGAATTTC CTCAACGTGGAGCACTACTTCCCCGTTGGCAGCACGATCTTCTTGATCAC GGATCCGTATTACGGCAGCGAGGGAACTGTGCAGGACCCACGGCTGGCCT ACACCAACGGCCGGATTCAAGTCAGCATCATGGTGCGACCGGAACCGAAA GTAAATGCGGCACGTCAGCTGCAGGAAGAGCGGGACCGGGACTATCTGAG CACCTTCCAGGTCTGCAACTTGCTCCGAATCAGTGGCCGCACCCTGGGAC GCCTAAGTGGCACCGTGTGGGTGGTGCTCGGTCCGCGGCGTCAGAAGATG GAAAACGTGACCAAGCACAACATTGGCCTGCAGCTCAAGTATCCGCGGCA GAACGAGGAGCGTGCCGGATACTGCTTCCGCACGAACAACCAGTGGTACT ACTCCAGCCTGGCCGTGGATCTGATGCGTAACTATTGCCAGCGTTACCCA GACGTTATCGACTTCTTTGGCGACTCCAACGATCGCGCCGAGTTCGTATT CGAACAGGACGTGTTCCCCAATGCGGTTGGCCATCGCCGCGTCGAGGAAC TGGCCAACTGGGTGCGCCAGCAGCCGCACATGAAAGTGGAGCGCATCTCT TGCGGCTCCAAGACGGTCTGCCGCGAGACCATCGAACTGCTGATAGCCGC CGTCGACGACCTGCGCTCACTGCCCGTCAAGCACGTTAAACTGCAGGTGA AGCCGCACTTGCTCATCAAGCCCAATGTCACACTGCCAGATGTCTACCGA TCAAAGCGCCCGGTGCGTCTCTTCGATCGCGTCGTAATCGTACGCACCAT TTACATGGTGCCCGTGGGCACTAAGGGCACCGTGATTGGCATTCATCCCG TTACGGACCCCAACCCGGTCCGCCTGGAGTGCGTCCATGCTGTCGACACG TTCTGCAAGGTGCTGTTCGACAGCCCCGTTCCGAACTGCAACAACATCCA TGGCATCGCCGAAGACCGAGTTTACAAGGTGCCCGAAATTGCCCTGGTGA TCATCAAGACGGATGAGGAAGGAAAGAAGCAGAACGACTGCGAACTGCCG GTGAGGGATCCTCAGCCGAACCAGGCACAGGACGAGCCAGTCCGCGCGAC CAGCAGTCGCTATGTGACCGCCGCAGGGTCCACCTCGGTCCCGATCACGA TGAAGACCCAAATTAGCGACGAATTCGTCAAGACGCGTAGCGATCCGATA GCCCGTACGGATTCGTACAAGCCATCGTCGGAGCCAAAACCGGTGCCGGT GCCGGAGCAGATTACCAACTGGCGGGAACGCGTCAGTACGCCAACAAACA AACCGCAGCCTGCGCCAAATAATTGGCGCATAAATCGGTCTTCATCACGT CAACAGGGCGGTAGTATTTTTGTTGCTCCGCCCACAAAAACTCCTGATGC AGCTGCAAGCACTGCTTCCACTGCATTCACCGCAGCCTCGTCCGCAACGC TGACTCCTCTGGATCAGACCTTGGCGCTCATGTCGGTTTTGGGCGTGGGT GAGGACCAGAGCTCGCCGCCACTCCAGGAGGCGGTGCAGCAGCAACGTCC GCCACTACTTCAACAGCAAAGAGCTCCGTTTCCCGGACAAATGCCCAATC TACCCAAGCCACCGCTCTTCTGGCAGCAAGAGGCGCAGAAGCAGGAGGCG CTGCAGCAGGAGGCGCAGCAGCAGGAGGCGCAGAAGAAGCAGCAGCAGGC GCATGCGCAAATGGAGCCTGAACGTATCAATAGCCAGCATTTCTACAGAA GCGGCCAGACAGGAGCGGCGTTGAATCAGCCGCCATTGGGTGCTCCATCA AAAAGGCAGTGGCATGAGTGGGTGCACCCGCGCATGCAGCACGCCAACGC ATTCCACGCTGGCGTCAACAATGGATATCAGATGCGTCCCAAAAAGAATA TAGCTGCGCAGAGCACCTTTAACAACAACGTGCACATGCACCTCCAGCAA CCATATTACCCGAATCAGCAGCAGCAGCAGCAGCAGCAGCAGCCACTCCA GCTGACGGAGATCAACAATGCGCCACCGCGATACTCAACGATCCAAGATT TTGTGCCCATACAGGCGTACCGTCCCAAGAAATTAAATCGCGTCCAACCA GCCGGTCGTCAGGACGTCGATGCCACCAAGAATCCTAGTCGTTCGCCTGT GTTGCAGCAACCGACGAACGAGACTATAGACACAAAGGCCAGTTCTAGTC TTCCGGTACAATCAGCAGGCGAACAGGTGATTGGACTGATGCAAACGCTT GAAATTAAGCCGGCTGCGTCGCAGTCCGAATCTGATGGGGTCTCAACAGG CAGTGCGAACGCGCCCACAGCAACGACATCATCCCAGGCGGTCAACCGAA GGAAGCATCGAGTGCCTCGCATCGGAGCCAAGTTCGACTTGGAATACATA CTGCCCGACTCCCCGCATCCAACTTAAGAGCGAACAAATGGGTATTTTTA GGTTCAAATCCCACCTAGCTGAAGAAATGCAACGCTATTTGGATCTTATA TCACCTTATTTCTATCTCTCCTAGTCTGTGCGAGGCACTAGAATTGCCGC ACACGATAGATCATTTTTTTTTTAGAGTTGCGGATTATGTGAATACTAAA ATGAGACTGACTCGTCTTTATATTTTTTCAATAAACTGGACTTTACCCAT AAAAAAAAAAAAAAAAAAAA
Library Information
Comments	mRNA source--0-22hr embryos, from an isogenic y; cn bw sp strain, polyA+ selected twice. cDNA made using Stratagene ZAP-cDNA synthesis kit; oligo(dT) primed with XhoI site at end of primer for first strand synthesis; EcoRI adapter on 5' ends of clones; size fractionated on Sephacryl S-500--approximately 1-6kb. For clones LD01001-LD21096, cDNA directionally cloned into EcoRI/XhoI-digested Stratagene Uni-Zap XR vector, which allows in vivo excision and recircularization of pBluescript SK(+/-) plasmid. For clones LD21101 and higher, cDNA directionally cloned into EcoRI/XhoI-digested pOT2 plasmid.
Library Synonyms
Reported As
Symbol Synonym	LD (Rubin et al., 2000) LD_pOT2 (Rubin et al., 2000)
Library References
Review	Rubin et al., 2000, Science 287(5461): 2222--2224 A Drosophila complementary DNA resource. [FBrf0127297]
Synonyms & Secondary IDs ( 0 )
Reported As
Symbol Synonym
Secondary FlyBase IDs

References ( 1 )
Review	Rubin et al., 2000, Science 287(5461): 2222--2224 A Drosophila complementary DNA resource. [FBrf0127297]