Clone Dmel\LD03603

General Information
Symbol	Dmel\LD03603	Species	D. melanogaster
Name	LD03603	FlyBase ID	FBcl0153938
Feature type	cDNA_clone	Created / Updated	2005-11-01/2006-08-19
Computed gene(s)	Dmel\lark

Collection Status	BDGP
Known Problems	none
Library	LD_pBS
Strain	y; cn bw sp
Tissue Source	embryonic stage whole organism
Vector	pBluescript_SK(-)
Sequence Data of the Insert
5' Sequence
Total bases	436
GenBank	AA203099
5' sequence data	GACGAAGTGCGAATCGATTTGGAAAAATCTGCTGCTCGGCCTGGAAAGTA GAAAGTATAAAGCTCTGCGTTTATAATTTAATTTGCAGCCAAAGCCCGCT GCCGATCAGCCGCCGCCGCCGCCGCCGCGTAGATATCCATTTTCCGGGAT TACCCCACCCAGAGTGATATAAACGATATAACCGTGGACGACCACGCAGC GGAACCCGCTGCCGCCATGCCCGGAGCCGGCACGTTCAAGTTATTCATCG GGAATCTTGACGAGAAGACACAGGCCACCGAGCTGCGTGCACTGTTCGAG AAGTACGGTACCGTCGTCGAGTGCGACGTGGTGAAGAACTATGGCTTCGT TCACATGGAGACGGAGCAGCAGGGTCGCGATGCCATACAGAATCTGAACG GTTATACGCTGAACGAGTTCGCCATCAAGGTGGAGG
Library Information
Comments	mRNA source--0-22hr embryos, from an isogenic y; cn bw sp strain, polyA+ selected twice. cDNA made using Stratagene ZAP-cDNA synthesis kit; oligo(dT) primed with XhoI site at end of primer for first strand synthesis; EcoRI adapter on 5' ends of clones; size fractionated on Sephacryl S-500--approximately 1-6kb. For clones LD01001-LD21096, cDNA directionally cloned into EcoRI/XhoI-digested Stratagene Uni-Zap XR vector, which allows in vivo excision and recircularization of pBluescript SK(+/-) plasmid. For clones LD21101 and higher, cDNA directionally cloned into EcoRI/XhoI-digested pOT2 plasmid.
Library Synonyms
Reported As
Symbol Synonym	LD (Rubin et al., 2000) LD_pBS (Rubin et al., 2000, )
Library References
Review	Rubin et al., 2000, Science 287(5461): 2222--2224 A Drosophila complementary DNA resource. [FBrf0127297]
External Crossreferences & Linkouts
Linkouts
	DGRC - Stock center for Drosophila cDNAs, vectors, and cell lines • FBcl0153938
Synonyms & Secondary IDs ( 0 )
Reported As
Symbol Synonym
Secondary FlyBase IDs

References ( 1 )
Review	Rubin et al., 2000, Science 287(5461): 2222--2224 A Drosophila complementary DNA resource. [FBrf0127297]