Clone Dmel\GM20958

General Information
Symbol	Dmel\GM20958	Species	D. melanogaster
Name	GM20958	FlyBase ID	FBcl0146217
Feature type	cDNA_clone	Created / Updated	2005-11-01/2006-08-19
Computed gene(s)

Collection Status	BDGP
Known Problems	none
Library	GM_pOT2
Strain	Oregon R
Tissue Source	oogenesis stage S1-S6 ovary
Vector	pOT2
Sequence Data of the Insert
Full Length Sequence
Total bases	2308
GenBank	BT003595
Full Length sequence data	AATCACATTTCGGGTAAAAACAAATTGAAATATCCACCGAATTTAAATAT TAAAGTTAGAGAAGCTGCCAAATATCTGGAGTTACAATCAGGGACAAGGA CAAGGACTACTACACTAGAGGAGAGCTTTTACAAGCAATCCGGTGAAACC ACATATAATGACAAGTCCGTCGCGGGATATAAACGATCTTTTTGACGATA TTGTCCTGACCGAGGAGAAGGAGGCACGTTTGGGCTACGAGGAGGGACTG AAGGACGGCCAGGAGCAGGGCAACGAAGAGGGCTATAAATTGGGCTACGC CCAGGGAGTATCCCTAGGCGAGGAGCTCGGCAAGATTCTGGGCCAGGTGG TGGCCCAGCAGCAGCTGAAACACACGGACAAGGTGCGTCGCAGCTTGGAA CAGTTGCGTTCGCTCATCGAGGAGTTTCCCCGCACCAACGATCCGCAGGC GGACATAGTGGGTGCTGTGCAGGACATTCGCTCCTCCCATCGTCGCCTTC GCGCCCAGCTGGGATCCAAAAAAACGCCTGGTGCAGCGCCTGCAGAGACC AATTCTGTCGAGCACAAGGACTACAGCTTCTAGCCTTATAGCACCATGTC CAAGGTGGGAGTTGTGCAGTTGCAGAGGCGTCTGGATGGCTTACTGGCCT TCCTCAATCCGCACTGGGATTTTGTCAATTGCCACATGGTGAACTACTTG ACGGACCAGCACTGGGACGGCTTTTTATCCGAAACGCTGAAAAGTGAGAT CGCCGGCAAGGAAGATGTGGCTCTGGCCATAGAAGACCTATTTTGGAAAA CTGATGAATCAGAAAGGTTTCCGGCATGGCGAGAATTCCTAGGCAAGAGC AAACAAGAACGCCTGTCGCTTCATCCGAAACTCCTGACCAGCGTGGAGGA ACTTATCGAGGGTCAGGGGAACTCCACGCAGCTAAGCATTCGGGAGTTTA TGAGCGCCAAAAAGTGCCATGAGGTGGATCTAACAGCCGTTCTCGTGGAC CAGCTTGTGAAGAACTCCGGTCAAGATTGCTTCATTGTAGACGCGGGCGA TGGCAAAGGGTATTTATCCTCGCGCTTGGCGCTCCAGTATGGTCACCGCG TGCTCGGCATCGATGCGAACGCGGCAAACACCGAGAACGCATTGAATAGG AATCGCAAATTGCAGCGCGCTTGGAATGGCCTTACGGAACGAGCGGAGCT GCAAGTCCAGGGAATCACGCCCAAGAGACGTAGTAAAAAGTCGCCAGCCA GGGAGAGCACCAAGAGTGCGCCTGCCTTGGAAAACTACAAGACAACGGCA AAGTTTATAACCACCGAACTTAACTTTGGTGCCCTGCTGGCGGAGAATTT CACTCAGTTTAGACCGGAAGACAGTCCAAACATTTGCCTTACTGGATTGC ATACCTGTGGCAACCTGGCCGCCACCTGTCTTCAGGTTTTCCACGCCCAA ACGGACTGCCGGTTGCTTTGTAATGTTGGCTGCTGCTATCATTTGCTGCG GGAGCGCTATTCGCAGCAGGAGTTCTTTGGCAATAAGTCCCTGATGGAGC TGCAAACAGACTATGGATTCCCATTGAGTCAGTACCTGCGGGAGCGACAA GTGCGAATGGGTCGCAATGCGCGGATGCTGGCGGCCCAGTCAATTGAACG CACCTTGGACACCAAGGAGCTGCCTAATGTAACGCTCTACTATCGAGCTC TCTTGGAGATTCTGGTTTGCCGCCATGCGCCGGAGCTGAAAAACGAACTG CAGGTGGGCAAGGTACGGAAATTCGAGAGTTTCGAGGAGTACATTCAAAA GTGTGCCACCAAATTGGATGCTCCCTGGTTGGCAGCTGTTGAGAAAGAAG AACTTCAATCCCTGCTCCAGGAATATGCCGTGCATAAATACTTCTTGGAT CTGTTCTATCTGCTGCGCATGTCCTTTGCTCCGGTGCTGGAGAGTCTAAT TCTCCTGGATCGCCTGCTCTACCTCAAGGAACTTGGCTATGAACGCAGCT ATCTGATAGATTTATTTGATCCCGTCATTTCGCCCAGGCATTTTGCAATT GTTTCTATAAAGCAGCAAACGCAACAATAATGAAATTCACAAATGCATCA AAAGCTTGACGAGAAAGCCGGTTGTGTTTAATTATTTATAGATTATAGCG TGCAATACTTTTCATATCGTATATGTATTGCTTATGTATGTAACGCTTTT AAAAATCTAACTAAACCATAGCACACAAAAAGTAAATAAGGTTGTTAAAA CTAAGAATCATTATAATAAATGTAATCATGACTTGTAAAAAAAAAAAAAA AAAAAAAA
Library Information
Comments	mRNA source--ovaries, stage 1-6 of oogenesis, from a non-isogenic Oregon R strain P2 population cage, polyA+ selected once, RNA provided by Allan Spradling. cDNA made using Stratagene ZAP-cDNA synthesis kit; oligo(dT) primed with XhoI site at end of primer for first strand synthesis; EcoRI adapter on 5' ends of clones; size fractionated on Sephacryl S-500--approximately 1-6kb. For clones GM01001-GM11496, cDNA directionally cloned into EcoRI/XhoI-digested Stratagene Uni-Zap XR vector, which allows in vivo excision and recircularization of pBluescript SK(+/-) plasmid For clones GM12101 and higher, cDNA directionally cloned into EcoRI/XhoI-digested pOT2 plasmid
Library Synonyms
Reported As
Symbol Synonym	GM (Rubin et al., 2000) GM_pOT2 (Rubin et al., 2000)
Library References
Review	Rubin et al., 2000, Science 287(5461): 2222--2224 A Drosophila complementary DNA resource. [FBrf0127297]
Synonyms & Secondary IDs ( 0 )
Reported As
Symbol Synonym
Secondary FlyBase IDs

References ( 1 )
Review	Rubin et al., 2000, Science 287(5461): 2222--2224 A Drosophila complementary DNA resource. [FBrf0127297]