Clone Dmel\GM12116

General Information
Symbol	Dmel\GM12116	Species	D. melanogaster
Name	GM12116	FlyBase ID	FBcl0144312
Feature type	cDNA_clone	Created / Updated	2005-11-01/2006-08-19
Computed gene(s)	Dmel\armi

Collection Status	N/A
Known Problems	chimeric clone
Library	GM_pOT2
Strain	Oregon R
Tissue Source	oogenesis stage S1-S6 ovary
Vector	pOT2
Sequence Data of the Insert
3' Sequence
Total bases
GenBank
3' sequence data
3' Sequence
Total bases	689
GenBank	CK131170
3' sequence data	CGGTGTNAGGAGCAATCTGAAACGGTGCATATAGGTTTTAACGCTTAGGG GTTCCTTNGAGGAATGAATAGTGCTGCTCGATGGCCTTGATCATCTCTTG TCGCCGTTCACTAGTCAAACAAATTTGGCGCAATTCTTTGGGCACTTCAA AGCAACCCAAACGCAGAGCAACAAATCTGCGCTTGGTAACAATTTGTTGT CCCGGGATTACATCCACCTTGTTGCACCAAACTTGGTTGGCATAGAACCG GGACCTCTCCTGGCTGTTACGACCGGGTGCCATCAAAGCTTCAGCTGCTA TCATACGTTTCTCAGTTTCAGCGACTTCCTCCTTGGTTTTGCACACGATC ACCGTGAAGACGCGCCTCACCTTGAGTAGATCGAAATTCAGCACGTACTT TTCGATTGCTTCCCCCAGGAACTGCGTATGGATCTCAAAGGTAACCGTTA TTTCGGAGCCGCTGGATATTTCCTTTGATTCGAGAGGGCTGACCACGCTC AACTGAGACTCACTGTCATTGCAGACGGTGATGCTCTCCAGGCGCAAAGT GCGGTTTAGATTGTTGGTCAGCTTTAAAGATACGCTCTCCTTTTGCCAGA GCTCTGCGGTGATGAAACGATTTACACCAGTCACGGTCACTGCTTTTCCA CTGCCGGATGATCCCATACTGCTCTGCTGAGTCAGCTTG
5' Sequence
Total bases
GenBank
5' sequence data
5' Sequence
Total bases	312
GenBank	AA736100
5' sequence data	TCACACTTAAAAGTTCGCCAAGACGCTTTGTGCTCAAAATTTTGCTCGAG AGTTTTTTCGAATACTATGACTATTTCCCATTGAACTCTTAAAACTCAAA GTGCAGTGCATCTACTAATTCAACAGCGTTAAAAATATTTCTTGATAACC AAGGTAAATCCGCGTGATTGGACAATGTCCAGCGAACGATCCGGAAGTAT AACCATCAAAGAAGAAGCATCTACAAGCGGTACGGCCACCAATATTCCCG TTGAATCCAGTGAAGCAGAGGCTAAGAAACCCGAGATCGGAGCTGACAAG GCGGAATCGCGA
Library Information
Comments	mRNA source--ovaries, stage 1-6 of oogenesis, from a non-isogenic Oregon R strain P2 population cage, polyA+ selected once, RNA provided by Allan Spradling. cDNA made using Stratagene ZAP-cDNA synthesis kit; oligo(dT) primed with XhoI site at end of primer for first strand synthesis; EcoRI adapter on 5' ends of clones; size fractionated on Sephacryl S-500--approximately 1-6kb. For clones GM01001-GM11496, cDNA directionally cloned into EcoRI/XhoI-digested Stratagene Uni-Zap XR vector, which allows in vivo excision and recircularization of pBluescript SK(+/-) plasmid For clones GM12101 and higher, cDNA directionally cloned into EcoRI/XhoI-digested pOT2 plasmid
Library Synonyms
Reported As
Symbol Synonym	GM (Rubin et al., 2000) GM_pOT2 (Rubin et al., 2000)
Library References
Review	Rubin et al., 2000, Science 287(5461): 2222--2224 A Drosophila complementary DNA resource. [FBrf0127297]
Synonyms & Secondary IDs ( 0 )
Reported As
Symbol Synonym
Secondary FlyBase IDs

References ( 1 )
Review	Rubin et al., 2000, Science 287(5461): 2222--2224 A Drosophila complementary DNA resource. [FBrf0127297]