Clone Dmel\GM03859

General Information
Symbol	Dmel\GM03859	Species	D. melanogaster
Name	GM03859	FlyBase ID	FBcl0143663
Feature type	cDNA_clone	Created / Updated	2005-11-01/2006-08-19
Computed gene(s)	Dmel\CG9925

Collection Status	BDGP
Known Problems	none
Library	GM_pBS
Strain	Oregon R
Tissue Source	oogenesis stage S1-S6 ovary
Vector	pBluescript_SK(-)
Sequence Data of the Insert
Full Length Sequence
Total bases	3182
GenBank	AY094745
Full Length sequence data	CCCAGCGAGCTTCGAATTTAAACGCGTTTAGTGAACGTATCGCATGTTTG TGAGTTGTGAATCGCGCACTTAAATTCTAAAGGATTCCACCTTTTTCAAA ACGAAAACATAGAAAGTTCTCGTCCAGCGTAAATAGATTTTCACTTTGTA TTTCATCGCGTGCTAAAAGTTCTGTTAGCGCCATTTTGCTGCGTTTCCTT TAACGCGCCGTTCTTTCATTCACACGTTCTTGCGTTGTTACTTTGAATTG AAGAAATGGAAAAGTCGGAGGAAAAGACATGCGTCGTCTGTGGCACTCCG ACCAGATTGATGTGCCAGCGGTGTGGAGAACCGTACTGCAATGAAAAGTG CCAGAAATTGGATTGGCAGAGGCACAGACAGGTGTGCATCATTATGCCAC CCTTGGTGGAGTGCCGGCCTCTTCAACCGGCGTTGAATCCAATCCAGAGC GTAGAGAACCAAGTTGCTCTGGCTAAAACTCGCACTAAATCGCCAATCCC ATGTGTGGAGCCCACAGTTGCTGTGCCGAACAAGCCGGATCTATCGGAAA ACTGGCGCAAACATTTGCTGCCGTCGGGCATGGAGTTTTTCAAGTGCCGC GTGACTTTCATGGAGAACGATGGACCCATTTGGGTGGTGCACGTGGCGAA TGTGGAGGCCATTGAGCGAATGACGGTCAACATGCAGCGCTGCATGCAGA ATAAGAAGATGATTCGTATGGAAGGAGTGCGGGAGGACACCCTCGTGGCC ATCAGTGTGGGCGACAAGGTCCACCGCGGTCATGTTTTGACGGTTTGCCA AAAGAAGCAAGAAGCCAATGTCCGTATGATTGACCACGGTCAGATAGTAG CTACACCGTTTCGGGATATCTACACCATTGTGCCCAAGATGGCCGAAAGC AAGGCGTTCGCTTTCCGTGTTCAATTGCCCACGAACACTGGAGTTCAGGA CATTAAGAACTTGACGCTCCGTATGTTGGGAACCAAAACCAGCGACGGAG CTTATCACGTCCATCTAAAGCCCAAGATGATAATACCACTAAGTTTGCCC TTGGAGATGCTGCAGCTTAATCCAGAAGTCAAAGTCATCCGGGTCTTTAA GGCAAATCCAAAGCATAACGAACCGCAGCTGGCCCTACTCCAGATCAACG TGATGGGCCATGTCAACGCAGACTTAAACAACCGCATAGCCGAGAAGCCA AGACAGCCATTCACGCTGCCTTTCCCCGAGCAGAAATCCATCTTCTTTGC GGCCACCCGCACTAAAAATGGTTATCGGCGAGCTTTCCTATTGGATCATA TCGTGAAACCGAGGCCAACCTATTTGGTTTACGAAATGGACGAGGGTAGA GTGTCCATAGCCACCGATCTGAGTCGTATTCCCAGCGAACTACTTGGCCT GCCCAGTCGAGTGTTTGCCGCTCAGCTGGAGGACTCCGTACCCGAGGAGC TTGAGACTCAAGGTGCAGAACTCACGGTCAAGTTTAAGCTGGATAATAAG CCGCCCAAGGAAAAGTTACGCGCGGCGAATGCCGCCTTGAATTCCAAGGG CGAGCAGATATGTATGGCCCGTCTGATCACTTTCTTGGGTCAGATTTCAT ATTTAGGCCACAAGTACTGGCGTGAACCCATCGTCCATGATTCCATCGTA TTCATTTCCCACTTGGTCAGCTTTAAGGAGGTATACATCAGCACTCCAGA CGCCAAGCAGTATGCGGAAATTTTCAAGCGCCTGGAGTACAAGTGCGCTA CCATTACCAAATCTAGTGATGTTTCCGTTGGCAGCATCGTGCTCGTTGTC TCCAAGCAGATGGGTCACTTCCGGGGCGAGATATTGAGTAAGGATAGTGG ATTGTTCGAAGTAATGAACGTTGATACTGGTGCCACCCAAAAGGTGGAGT TGGCGGAAATACGCAGCTCCTGTCGCTTCCTGGAGAATATGCCCGTATGT CTGATGCGTGTACAGTTGCAGAATGTCTGCAATATTCCGGATGCAGCTGT GCCAGTTAACAATGCGGCCATTCAAATGCTGCACAAGCTCTGCGCCCAAA AAGAACTGCTAAAATTGAACATGGTAGATGCAACTACATCGACCGTGGAC CTGCTGTTCAGCTCCGGCGATTCGCGCTCCTTGACGACCCAAATGTTGCC TCTTATATTTACACCGGTACAGGAAAAAGCAGAAGTTGCACCACCGAAGG CTGCTTCTACTCCGAGTCAATTGCCTGTTGTAACTCACAAACAGCAGAAG GTGTCTCCACAGTCTCTACTTCTGCTGGATCTGCCTCCACTGCCGCCCTC TCCACCGGAGTCGCCTTCTCCTGCCGACGTGCCAACTAAAAAGTCAGCTT CCCATGTTCTGCCCATTAAACGCTTCCTTTTCGACGCTTTGCCCAAGAAT CTAGCTCCTTTAGGCGACAAGGTCAACCTAATCCTTATGAACGCTGACGG ATTGCCGCAAACTGGCTACATCACCGCCGCCTACTTCAAGGACGAAAAGG CGGCCAAGGAATTCGAAAAAATCCTTAGCTTGACATCAAGTCAGGGAGCA TGTGACCACAATGTTGTTCCGGGTTACGTACCAAACGTTGGTGAATTGTG CCTGGCGATCTACAGCGAAGATAAGAATTGGTATCGCGGAGTCTGCCAGG AGGTTAAGGACAATATGGTCAAAATTCTATTCTGCGATTTTGGAAATACG GAATATGTGGCCGTGAGACACGTGAAACCGATTTCACAGGATTTGCTAAT TGCCGTCAATGTCACAAAGTGCTATATAAATGGCTTCGACAAATCAAAGA ATTTTGCGGCTCTGGAGCAGTTTCTTGTGCGTAAGATTCGATTCTTATGC GGAGTTAAGAAAGGCCCTGAACCCGATACTCGTCTCATTACAATTCCGGA TATGGAGACTATTCTAAACACACCAGTTGCATAATTTAACTTAAGAACTC GCATTCCTTCATATGACTTTAGCATTAAGTGTACACCAAAAGTGTATGTT CAAACATTATTTCGATATTCGATATAAGTTTCGACCACAGTGTATTTTCG AACATGATTTTGACAATTATTCGCTTACATTTATTCCAGCACAGAGTGAT TCGACTAAGAGACTTGACACCATATAAAGATGTTGATCGTTCATACAGAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA
Library Information
Comments	mRNA source--ovaries, stage 1-6 of oogenesis, from a non-isogenic Oregon R strain P2 population cage, polyA+ selected once, RNA provided by Allan Spradling. cDNA made using Stratagene ZAP-cDNA synthesis kit; oligo(dT) primed with XhoI site at end of primer for first strand synthesis; EcoRI adapter on 5' ends of clones; size fractionated on Sephacryl S-500--approximately 1-6kb. For clones GM01001-GM11496, cDNA directionally cloned into EcoRI/XhoI-digested Stratagene Uni-Zap XR vector, which allows in vivo excision and recircularization of pBluescript SK(+/-) plasmid For clones GM12101 and higher, cDNA directionally cloned into EcoRI/XhoI-digested pOT2 plasmid
Library Synonyms
Reported As
Symbol Synonym	GM (Rubin et al., 2000) GM_pBS (Rubin et al., 2000, )
Library References
Review	Rubin et al., 2000, Science 287(5461): 2222--2224 A Drosophila complementary DNA resource. [FBrf0127297]
External Crossreferences & Linkouts
Linkouts
	DGRC - Stock center for Drosophila cDNAs, vectors, and cell lines • FBcl0143663
Synonyms & Secondary IDs ( 0 )
Reported As
Symbol Synonym
Secondary FlyBase IDs

References ( 1 )
Review	Rubin et al., 2000, Science 287(5461): 2222--2224 A Drosophila complementary DNA resource. [FBrf0127297]