Clone Dmel\GM05518

General Information
Symbol	Dmel\GM05518	Species	D. melanogaster
Name	GM05518	FlyBase ID	FBcl0139269
Feature type	cDNA_clone	Created / Updated	2005-11-01/2006-08-19
Computed gene(s)	Dmel\Dcp2

Collection Status	BDGP
Known Problems	none
Library	GM_pBS
Strain	Oregon R
Tissue Source	oogenesis stage S1-S6 ovary
Vector	pBluescript_SK(-)
Sequence Data of the Insert
5' Sequence
Total bases	503
GenBank	AA802413
5' sequence data	AGAACGAAGACCCAGCCCATTGTGCACGAGAGAGGTTTATGAGGAGACCG GGTTCGATATCACGGACCTAATCGATGCCAACGACTACATCGAGGCGTTC ATCAACTACCAGTACACGCGGCTGTACGTGGTGCGAAACATACCGATGGA CACGCAGTTCGCGCCCCGCACTCGCAACGAGATCAAGTGCTGCGACTGGT TCCGTATCGATGCGCTGCCGGTGAACAAGAACGATGCCATATCAAAGGCC AAGTTGGGCAAGACCTCCAACTCCTTCTTCATGATTATGCCGTTTGTGAA GCGCCTGAAGAAGTGGGTGAACGATCGGAAGGCTGGAATCGAGCCGCGTC GCCGCAAATTCTCTGGTCAGCAATCGCCAAAAGCGGCCTCGCCCACCAAT ATGAACGGAAATTGCCAGAAGGACGTCACAGGAGTGCGAAATGAATCGCA ACGACAGAGGGCACAGTCCATGGGCGACTTGGATGGCGTTCAATTGANTA ATC
Library Information
Comments	mRNA source--ovaries, stage 1-6 of oogenesis, from a non-isogenic Oregon R strain P2 population cage, polyA+ selected once, RNA provided by Allan Spradling. cDNA made using Stratagene ZAP-cDNA synthesis kit; oligo(dT) primed with XhoI site at end of primer for first strand synthesis; EcoRI adapter on 5' ends of clones; size fractionated on Sephacryl S-500--approximately 1-6kb. For clones GM01001-GM11496, cDNA directionally cloned into EcoRI/XhoI-digested Stratagene Uni-Zap XR vector, which allows in vivo excision and recircularization of pBluescript SK(+/-) plasmid For clones GM12101 and higher, cDNA directionally cloned into EcoRI/XhoI-digested pOT2 plasmid
Library Synonyms
Reported As
Symbol Synonym	GM (Rubin et al., 2000) GM_pBS (Rubin et al., 2000)
Library References
Review	Rubin et al., 2000, Science 287(5461): 2222--2224 A Drosophila complementary DNA resource. [FBrf0127297]
Synonyms & Secondary IDs ( 0 )
Reported As
Symbol Synonym
Secondary FlyBase IDs

References ( 1 )
Review	Rubin et al., 2000, Science 287(5461): 2222--2224 A Drosophila complementary DNA resource. [FBrf0127297]