Clone Dmel\GM10845

General Information
Symbol	Dmel\GM10845	Species	D. melanogaster
Name	GM10845	FlyBase ID	FBcl0138251
Feature type	cDNA_clone	Created / Updated	2005-11-01/2006-08-19
Computed gene(s)	Dmel\armi

Collection Status	BDGP
Known Problems	none
Library	GM_pBS
Strain	Oregon R
Tissue Source	oogenesis stage S1-S6 ovary
Vector	pBluescript_SK(-)
Sequence Data of the Insert
5' Sequence
Total bases	288
GenBank	AA803921
5' sequence data	ACGCTTTGTGCTCAAAATTTTGCTCGAGAGTTTTTTCGAATACTATGACT ATTTCCCATTGAACTCTTAAAACTCAAAGTGCAGTGCATCTACTAATTCA ACAGCGTTAAAAATATTTCTTGATAACCAAGGTAAATCCGCGTGATTGGA CAATGTCAGCGAACATCCGGAAGTATAACCATCAAAGAAGAAGCATCTAC AAGCGGTACGGCCACCAATATTCCCGTTGAATCCAGTGAAGCAGAGGCTA AGAAACCCGAGATCGGAGCTGACAAGGCGGAATCCAGA
Library Information
Comments	mRNA source--ovaries, stage 1-6 of oogenesis, from a non-isogenic Oregon R strain P2 population cage, polyA+ selected once, RNA provided by Allan Spradling. cDNA made using Stratagene ZAP-cDNA synthesis kit; oligo(dT) primed with XhoI site at end of primer for first strand synthesis; EcoRI adapter on 5' ends of clones; size fractionated on Sephacryl S-500--approximately 1-6kb. For clones GM01001-GM11496, cDNA directionally cloned into EcoRI/XhoI-digested Stratagene Uni-Zap XR vector, which allows in vivo excision and recircularization of pBluescript SK(+/-) plasmid For clones GM12101 and higher, cDNA directionally cloned into EcoRI/XhoI-digested pOT2 plasmid
Library Synonyms
Reported As
Symbol Synonym	GM (Rubin et al., 2000) GM_pBS (Rubin et al., 2000)
Library References
Review	Rubin et al., 2000, Science 287(5461): 2222--2224 A Drosophila complementary DNA resource. [FBrf0127297]
Synonyms & Secondary IDs ( 0 )
Reported As
Symbol Synonym
Secondary FlyBase IDs

References ( 1 )
Review	Rubin et al., 2000, Science 287(5461): 2222--2224 A Drosophila complementary DNA resource. [FBrf0127297]