Clone Dmel\GH27691

General Information
Symbol	Dmel\GH27691	Species	D. melanogaster
Name	GH27691	FlyBase ID	FBcl0121561
Feature type	cDNA_clone	Created / Updated	2005-11-01/2006-08-19
Computed gene(s)	Dmel\CG31781

Collection Status	DGC gold BDGP
Known Problems	none
Library	GH
Strain	y; cn bw sp
Tissue Source	adult stage head
Vector	pOT2
Sequence Data of the Insert
Full Length Sequence
Total bases	2341
GenBank	AY075352
Full Length sequence data	TGGTAGCGTCTGGTGTGGCGAGCCAACAAAAGGCTGGCAGAGCGGCCAAC ACCGGACCCGGCCCGAAACTGAATCCGGGTCCGGGTCTGGGTCCGTGTCC GTGTCCGGGTTGGTTTCGCAGCATCTACGGCATCACGGCTGCCAATTGAG CGGAGTTTGGGTCTCAATTTGACGCGTAGCCAGCCAAGAAGAGTGGCCGG CCAACAACAAAAACAGGTGACGCAGGATGTGGCCAACTGAGTAGGGAGAT GGCGGAAGAGCAGGAGGCGCAGGAGGAGGAGGAGGAGGAGGAGGAGGAGG AAACGCAGCACACTTTTTTGGCCACGGTCCGCGTTTGATTAGCCAACTCA AACATCAGGGCCAAGATCAGGGCAATTGACAGCTCTGCTCCCTCAGTTTC TGCTTCCGCTTGGCACACAGTCACAGAGACACACATTGCCTTATGGCCAC TGGACGATGGACCACAGTGGAGCCTGGGCAGTACGCAACACCTGACGCTG CCCGCCAGGATACTCCCGTTCGTCTCGATCATACAGCAAATAAGAAACAT TAAATAAACAATCAAAGAAACGGAGTATTGCTCCGCAACTGCCAACAACA TTCAACGACAACCAGTTGCTCTGTGGTGCTAGAAAGTGAGTGTAAACAGT GTGTGCCTTTGTGAACTGAACTGACCAACGTGCCAAGAACGAAGGGACGA GGAGGTGGGCACTCTGGCTCGGACCCAAGCGATTTGGCAGGAGCTGATTG TTTCATCTGGCCAGGCTTAATGAAATAACTCGTCCTGGCCGCGGCTCCAT GTCAAACGCAGCAGGGCAATCGCCAGCGAAGCCCCAAAGGATAAGCATCG TCCAGAACATCGGAATCTCCAGCGAAGGATGCACCAAAAACATTATCCAC AGGACTAAGATGCCAAAGGAAACTTTCAAGATCCGTTTCCAAAACAAAAC ATAAAATATATTCAATAAAAATGAAGTGGAAATGAAATGATACATAATAA ATACCCTAATACATAACAGAAACAAAAGCAAGTCACTAAACTTGACTGCA AATATAATACGATGACACTTAATAGAAACATTGTAATAAAATTGAAAAGA AGTAGACAAGAATAACCACTCATAAAACTAAAATACCCAGCCTATAACTA TTTTTCAAATAGTTGGCATAGTGCTTACTATGAACCATAATCGCATATGA TATAACCAATATTGAGCACTTAATATAGCCCAATATTATATAGACCCAAA TATGAAATAGTCACAAACAATAGTGCAATACAATACGTTTATAAAACAAC GTCAAAAGTTTTATAACGAAAATATATTTCTTTATAATGAGCACTTTCGA ACAACTACATTTAATATAAAATAGCGCGGGAAGTCTTCGGCGTGGTGAAA GTATAAGGTGTAAGCACAGCTGAGAAGAAAGGAATTTACAAAATCGAAGG AAGAAGTGAAAGAAGGAGTGAAAAAACATCTAAGCGCGGAAAAGTGCTAC AAAATGAAGCTGACATCCTAAAGGAAACCTTGTTGGCCAATTGGGGAGGA GGCCCAGAAGGTCGGGCCGATTTTCCGCTGCAACCGACGAAGGTCTGACC AACCAATCGCAACGCACAGCGGCGACGAAGCCCGGCCGAATGGGAGCATC TCGGGCAAACGGAGGGACAGTCAGCCGCCCTGCAATGCATTTCTTTTGGC AACGAGTCAAGCGGCCTTTCACACACACACACACACACATTAGGATTCCA TAAGGATACTCTGCCTCGCTCACATGCGCATCGAGTGGTATACGTAATAT ATCAAGTATACGCCTGTTGGCCGGGCAGAAAGTCTGTGAGTGTGTGCGAA TGGCCAAACAGAGTGTTGCCAACTGCATTGCCGGGCTGCCAACGACTCAA CCCCCGACCCGAGGACACCACCCTCCTCCCGGCCAGGAGGCCAACGACGA GTCCTTTTTGGAGGCAGCGCATTGGCTGCAGGACGACCGGCGACAGGACG ACCAATCACCAATCAAATCGCGGCTTGTGCGCACTATCACGGCCGTACGA AGGGCCAAAGGCACGGCATCCAGGACCTCAGCCGGAGACCCCAAGCCGAT TGTCAATCTCTTAGACATATTATTATGCCAACGTCTCCTATATTATAGTT CTCTAGTCGTAAGTCACATGCCCAAGCCATCGCCCCAGAACATCGGGGAC ATGAAACATTAGTTTTATTGATAGAGTTCTTAGTTTCGAGCAGAAGCTAT ACGATGATGATTTCAAGAATGAGAAAACGGAATAAAAACTGTAAAGCTTC AGAGCGGAACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA
Library Information
Comments	The GH library is separate from the HL library. Since many of the HL clones were not full length and others lacked inserts, we chose to remake the head library. mRNA source--adult heads, from an isogenic y; cn bw sp strain, polyA+ selected once. Oligo(dT) primed with XhoI site at end of primer for first strand synthesis; EcoRI adapter on 5' ends of clones; size fractionated on Sephacryl S-500--approximately 1-6kb. cDNA directionally cloned into EcoRI/XhoI-digested Stratagene Uni-Zap XR vector, which allows in vivo excision and recircularization of pBluescript SK(+/-) plasmid.
Library Synonyms
Reported As
Symbol Synonym	GH (Rubin et al., 2000, )
Library References
Review	Rubin et al., 2000, Science 287(5461): 2222--2224 A Drosophila complementary DNA resource. [FBrf0127297]
External Crossreferences & Linkouts
Linkouts
	DGRC - Stock center for Drosophila cDNAs, vectors, and cell lines • FBcl0121561
Synonyms & Secondary IDs ( 0 )
Reported As
Symbol Synonym
Secondary FlyBase IDs

References ( 1 )
Review	Rubin et al., 2000, Science 287(5461): 2222--2224 A Drosophila complementary DNA resource. [FBrf0127297]