Clone Dmel\GH11392

General Information
Symbol	Dmel\GH11392	Species	D. melanogaster
Name	GH11392	FlyBase ID	FBcl0111243
Feature type	cDNA_clone	Created / Updated	2005-11-01/2006-08-19
Computed gene(s)	Dmel\pcm

Collection Status	BDGP
Known Problems	none
Library	GH
Strain	y; cn bw sp
Tissue Source	adult stage head
Vector	pOT2
Sequence Data of the Insert
3' Sequence
Total bases	606
GenBank	AI134050
3' sequence data	ACATTTGAAATTAGCCACAGATTGCGTGGCACTGACAGCTTTATTCAAAA CAAAATACGTCTTATAGGACAGATCCTTCTTAAAAGCTAATTTACACAAT GCAGCTTGGCATTCTATCGAGTAACAACAAAATATGGGTAAAGTCCAGTT TATTGAAAAAATATAAAGACGAGTCAGTCTCATTTTAGTATTCACATAAT CCGGAACTCTAAAAAAAAAATGATCTATCGTGTGCGGCAATTCTAGTGCC TCGCACAGACTAGGAGAGATAGAAATAAGGGGATATAAGATCCAAATAGC GTTGCATTTCTTCAGCTAGGTGGGATTTGAACCTAAAAATACCCATTTGT TCGCTCTTAAGTTGGATGCGGGGAGTCGGGCAGTATGTATTCCAAGTCGA ACTTGGCTCCGATGCGAGGCACTCGATGCTTCCTTCGGTTGACCGCCTGG GATGATGTCGTTGCTGTGGGCGCGTTCGCACTGCCTGTTGAGACCCCATC AGATTCGGACTGCGACGCAGCCGGCTTAATTTCAAGCGTTTGCATCAGTC CAATCACCTGTTCGCCTGCTGATTGTACCGGAAGACTAGAACTGGCCTTT GTGTCT
Library Information
Comments	The GH library is separate from the HL library. Since many of the HL clones were not full length and others lacked inserts, we chose to remake the head library. mRNA source--adult heads, from an isogenic y; cn bw sp strain, polyA+ selected once. Oligo(dT) primed with XhoI site at end of primer for first strand synthesis; EcoRI adapter on 5' ends of clones; size fractionated on Sephacryl S-500--approximately 1-6kb. cDNA directionally cloned into EcoRI/XhoI-digested Stratagene Uni-Zap XR vector, which allows in vivo excision and recircularization of pBluescript SK(+/-) plasmid.
Library Synonyms
Reported As
Symbol Synonym	GH (Rubin et al., 2000)
Library References
Review	Rubin et al., 2000, Science 287(5461): 2222--2224 A Drosophila complementary DNA resource. [FBrf0127297]
Synonyms & Secondary IDs ( 0 )
Reported As
Symbol Synonym
Secondary FlyBase IDs

References ( 1 )
Review	Rubin et al., 2000, Science 287(5461): 2222--2224 A Drosophila complementary DNA resource. [FBrf0127297]