Clone Dmel\GH05133

General Information
Symbol	Dmel\GH05133	Species	D. melanogaster
Name	GH05133	FlyBase ID	FBcl0107061
Feature type	cDNA_clone	Created / Updated	2005-11-01/2006-08-19
Computed gene(s)	Dmel\Dcp2

Collection Status	N/A
Known Problems	none
Library	GH
Strain	y; cn bw sp
Tissue Source	adult stage head
Vector	pOT2
Sequence Data of the Insert
5' Sequence
Total bases
GenBank
5' sequence data
5' Sequence
Total bases	646
GenBank	AI107445
5' sequence data	AGAGAGACGCGAATGTGAATTAACAAACAAACAAAAATATTTTGCGAAAA AGACAAACACAAAAAGTAAAGCCAATAAAGTGTATTACATAAACAAACGG AGCTCCGATATCTAAATAAATATTATGGAAATCGCACCACTGATCAATAA CGCCGTCGCTGTCGTCACAGCCTCTGCCTCTGCCGCCGTCTCTGCCTCTG CTAGCGTCGGCAGTAGCAGCAAGGATGATAACGTCAACAATCTAGCGTTG CTCGCGTCGCTGACTTTACAAAAAGTGCTAAATATCAACAATAACAATAA CCAGCACAGCAAGAACAACAACCACAGCGACAATAGCAATAGCAAACAAC ACGAGCAACAAAAACAGCAGCAGCAACCACAACGACGAAACCTCGACATT AAAAGCGAGTATGATGTACTAAACCAATTGCTAAAAACTGGAGCTGTTTT CACCGCCACGCCCACAGCGCCAATTGCCATCGAAAGGAGGAAGCAGACGC AGCAGCAGCAACGTCCATATGCACAACAGGCACATCAACAGCAGCAGCAG AAGCAACGCCTAAACTCATCCATATCATCGACCCACTCATCGACATCGAC AAACTCCTCCTACGGCAGCTCATCATCGGAGGATGCTGCAAGCACA
3' Sequence
Total bases
GenBank
3' sequence data
3' Sequence
Total bases	709
GenBank	BG632211
3' sequence data	CTTTTTGGTATGAAAATCTTGTTCTTTCTCGTATTAAAAATGTCCTATAC AATTGATCGCATTCAATTTTCGAGTTAAGGTGTGTTTGTGGTTCCCTAAC GTAAAATATATATATATGTCTGGAAAAGCTTGATCTCCAGTACAGAGATC GAGAAACTTTACTTATATACATAGTCTCCAAGGCCTAGGACTAGACATGA TTAGTTGTCTATATAGTGTCAGTAGTTGTAGTGTTAGTTTTATAACAAAT GAACTTGTTAAATGCAATTGGCATGCTTTCATTTACTGGCAAGTCTCCAA AGTTTCTGTTACCCAAAGCGCGAGTCCTTAAACTCCACAGAGCCTCCTAT ACTATTCGGTGTTCCCGGTAACCGGGCCTTTTGCGCGGAAATGCATTAAT TGAGAGTTTTAAAAGGAAGTGTGTGTGATTGACTCAAAGAAAAGTTTAGC AAAACACATTTGCTATGAAGTTCTTGGTAAAGGAGAAATTCGTCCACGAA TTCAAATTTGGCGTAAAGTCGATTCCCANCTGCTGCTGCTGCTGTTGTTG CTGTTCTATTTGCAACTGTTGCTGATGGTGCTGCTGCGGCTGCTGCGACT GTGCCCGCTTCAGAATCCTCAAATTTGTGCCCGATGCCATGCGCTGCAGC CTGTGCATGTACTGGGCATCCGGAGCTGATGTTGTTGCTCCTGTGGCACT GCCTGCTCC
Library Information
Comments	The GH library is separate from the HL library. Since many of the HL clones were not full length and others lacked inserts, we chose to remake the head library. mRNA source--adult heads, from an isogenic y; cn bw sp strain, polyA+ selected once. Oligo(dT) primed with XhoI site at end of primer for first strand synthesis; EcoRI adapter on 5' ends of clones; size fractionated on Sephacryl S-500--approximately 1-6kb. cDNA directionally cloned into EcoRI/XhoI-digested Stratagene Uni-Zap XR vector, which allows in vivo excision and recircularization of pBluescript SK(+/-) plasmid.
Library Synonyms
Reported As
Symbol Synonym	GH (Rubin et al., 2000)
Library References
Review	Rubin et al., 2000, Science 287(5461): 2222--2224 A Drosophila complementary DNA resource. [FBrf0127297]
Synonyms & Secondary IDs ( 0 )
Reported As
Symbol Synonym
Secondary FlyBase IDs

References ( 1 )
Review	Rubin et al., 2000, Science 287(5461): 2222--2224 A Drosophila complementary DNA resource. [FBrf0127297]