Clone Dmel\bs14a05

General Information
Symbol	Dmel\bs14a05	Species	D. melanogaster
Name	bs14a05	FlyBase ID	FBcl0057225
Feature type	cDNA_clone	Created / Updated	2005-11-01/2006-08-19
Computed gene(s)	Dmel\CG4691

Collection Status	N/A
Known Problems	none
Library	BS
Strain	y w [67cl]
Tissue Source	adult stage testis & seminal vesicle & ejaculatory duct
Vector	pBluescript_SK(-)
Sequence Data of the Insert
Full Length Sequence
Total bases	319
GenBank
Full Length sequence data	GCACCAGGGGAGCACATCTAAAACAAAAAAAATGCGGTGCGGTCAGTAGT TAAATTGCGAAATTGAAATCGAAGTAAGCTCGTGGCGTCTTACCAGTTTT ACCCAAAATTATCTCGTTTTGATTTGAAGACTCGCTGCAAAATAATTTTA ATCAAACAAAATTAAAATGCTTGCCAAAAGCAAACCGCTGATTGGCATGT CTCACTTGCTGCAAAAGCAGGTTTTGGGCTTTCTGCCTCCGTCTTCGTTT CGCCACTTTAACTCCGAGGGCAATTTGTACTCCCAGGATGGCTGGGAGAG TGGCTATCCGGCGCCCTTG
Library Information
Comments	A testis Drosophila cDNA library was constructed by Justen Andrews in Brian Oliver's laboratory at the National Institute of Diabetes and Digestive and Kidney Diseases (NIDDK) which is part of the National Institutes of Health (NIH) at Bethesda, MD, USA. Poly (A) + RNA was prepared from dissected testes from 1-5 day old y w [67cl] Drosophila melanogaster flies, after removal of the paragonia and inclusion of the ejaculatory ducts and seminal vesicles. The size fractionated cDNA (1-6kb) was directionally cloned in the Stratagene Uni-Zap XR vector using EcoRI and XhoI restriction sites. pBluescript SK (-) is produced after excision. The host strain is E.coli SOLR.
Library Synonyms
Reported As
Symbol Synonym	BS
Library References
Research paper	Andrews et al., 2000, Genome Res. 10(12): 2030--2043 Gene discovery using computational and microarray analysis of transcription in the Drosophila melanogaster testis. [FBrf0132348]
External Crossreferences & Linkouts
Linkouts

Synonyms & Secondary IDs ( 0 )
Reported As
Symbol Synonym
Secondary FlyBase IDs

References ( 0 )
Research paper	Andrews et al., 2000, Genome Res. 10(12): 2030--2043 Gene discovery using computational and microarray analysis of transcription in the Drosophila melanogaster testis. [FBrf0132348]